Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10120

Experiment: inner cut, LB soft agar motility assay

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBPHYT_RS10115 and BPHYT_RS10120 are separated by 275 nucleotidesBPHYT_RS10120 and BPHYT_RS10125 are separated by 160 nucleotides BPHYT_RS10115: BPHYT_RS10115 - hypothetical protein, at 2,304,178 to 2,304,921 _RS10115 BPHYT_RS10120: BPHYT_RS10120 - membrane protein, at 2,305,197 to 2,305,748 _RS10120 BPHYT_RS10125: BPHYT_RS10125 - hypothetical protein, at 2,305,909 to 2,306,361 _RS10125 Position (kb) 2305 2306Strain fitness (log2 ratio) -1 0 1 2at 2304.676 kb on + strand, within BPHYT_RS10115at 2304.765 kb on + strand, within BPHYT_RS10115at 2304.852 kb on + strandat 2304.852 kb on + strandat 2304.852 kb on + strandat 2304.864 kb on + strandat 2304.998 kb on + strandat 2304.998 kb on + strandat 2304.999 kb on - strandat 2305.102 kb on + strandat 2305.102 kb on + strandat 2305.196 kb on + strandat 2305.261 kb on + strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.262 kb on - strand, within BPHYT_RS10120at 2305.806 kb on + strandat 2306.194 kb on + strand, within BPHYT_RS10125at 2306.478 kb on - strandat 2306.478 kb on - strandat 2306.702 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
remove
2,304,676 + BPHYT_RS10115 0.67 +0.5
2,304,765 + BPHYT_RS10115 0.79 +1.0
2,304,852 + +0.4
2,304,852 + -0.2
2,304,852 + +0.4
2,304,864 + +0.0
2,304,998 + +0.7
2,304,998 + -0.4
2,304,999 - -0.8
2,305,102 + -0.1
2,305,102 + +0.0
2,305,196 + -0.1
2,305,261 + BPHYT_RS10120 0.12 +0.0
2,305,262 - BPHYT_RS10120 0.12 +1.7
2,305,262 - BPHYT_RS10120 0.12 +1.9
2,305,262 - BPHYT_RS10120 0.12 +0.1
2,305,806 + -0.6
2,306,194 + BPHYT_RS10125 0.63 -0.7
2,306,478 - -0.5
2,306,478 - +0.8
2,306,702 - -0.0

Or see this region's nucleotide sequence