Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04185 and BPHYT_RS04190 are separated by 95 nucleotides BPHYT_RS04190 and BPHYT_RS04195 are separated by 416 nucleotides BPHYT_RS04195 and BPHYT_RS04200 are separated by 55 nucleotides
BPHYT_RS04185: BPHYT_RS04185 - diguanylate cyclase, at 959,872 to 961,116
_RS04185
BPHYT_RS04190: BPHYT_RS04190 - AraC family transcriptional regulator, at 961,212 to 962,168
_RS04190
BPHYT_RS04195: BPHYT_RS04195 - molecular chaperone GroES, at 962,585 to 962,875
_RS04195
BPHYT_RS04200: BPHYT_RS04200 - molecular chaperone GroEL, at 962,931 to 964,571
_RS04200
Position (kb) 961
962
963 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 960.276 kb on + strand, within BPHYT_RS04185 at 960.277 kb on - strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.278 kb on + strand, within BPHYT_RS04185 at 960.279 kb on - strand, within BPHYT_RS04185 at 960.380 kb on - strand, within BPHYT_RS04185 at 960.438 kb on - strand, within BPHYT_RS04185 at 960.470 kb on + strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.703 kb on - strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.710 kb on + strand, within BPHYT_RS04185 at 960.711 kb on - strand, within BPHYT_RS04185 at 960.756 kb on - strand, within BPHYT_RS04185 at 961.040 kb on + strand at 961.040 kb on + strand at 961.041 kb on - strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.224 kb on + strand at 961.225 kb on - strand at 961.225 kb on - strand at 961.267 kb on - strand at 961.356 kb on + strand, within BPHYT_RS04190 at 961.380 kb on + strand, within BPHYT_RS04190 at 961.381 kb on - strand, within BPHYT_RS04190 at 961.385 kb on - strand, within BPHYT_RS04190 at 961.672 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.766 kb on + strand, within BPHYT_RS04190 at 961.767 kb on - strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.905 kb on + strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.906 kb on - strand, within BPHYT_RS04190 at 961.907 kb on + strand, within BPHYT_RS04190 at 962.167 kb on - strand at 962.281 kb on - strand at 962.327 kb on - strand at 962.327 kb on - strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.395 kb on - strand at 962.395 kb on - strand at 962.403 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 960,276 + BPHYT_RS04185 0.32 +0.5 960,277 - BPHYT_RS04185 0.33 +0.1 960,278 + BPHYT_RS04185 0.33 -1.7 960,278 + BPHYT_RS04185 0.33 +0.3 960,278 + BPHYT_RS04185 0.33 +0.4 960,278 + BPHYT_RS04185 0.33 -3.8 960,278 + BPHYT_RS04185 0.33 +0.4 960,279 - BPHYT_RS04185 0.33 -0.1 960,380 - BPHYT_RS04185 0.41 +0.0 960,438 - BPHYT_RS04185 0.45 -0.6 960,470 + BPHYT_RS04185 0.48 -0.2 960,703 - BPHYT_RS04185 0.67 -0.6 960,703 - BPHYT_RS04185 0.67 -0.3 960,703 - BPHYT_RS04185 0.67 +0.4 960,703 - BPHYT_RS04185 0.67 -0.1 960,710 + BPHYT_RS04185 0.67 +0.5 960,710 + BPHYT_RS04185 0.67 +1.0 960,710 + BPHYT_RS04185 0.67 -1.1 960,711 - BPHYT_RS04185 0.67 +0.3 960,756 - BPHYT_RS04185 0.71 -0.7 961,040 + +1.7 961,040 + -0.4 961,041 - +1.2 961,224 + +0.2 961,224 + -1.1 961,224 + +0.3 961,225 - -0.9 961,225 - +0.1 961,267 - -0.0 961,356 + BPHYT_RS04190 0.15 -0.3 961,380 + BPHYT_RS04190 0.18 +1.4 961,381 - BPHYT_RS04190 0.18 -0.0 961,385 - BPHYT_RS04190 0.18 +0.1 961,672 + BPHYT_RS04190 0.48 +0.6 961,766 + BPHYT_RS04190 0.58 +0.0 961,766 + BPHYT_RS04190 0.58 +1.0 961,766 + BPHYT_RS04190 0.58 -0.1 961,767 - BPHYT_RS04190 0.58 +0.6 961,905 + BPHYT_RS04190 0.72 +0.8 961,905 + BPHYT_RS04190 0.72 +0.6 961,906 - BPHYT_RS04190 0.73 +0.3 961,906 - BPHYT_RS04190 0.73 +0.4 961,907 + BPHYT_RS04190 0.73 -0.9 962,167 - +0.1 962,281 - +0.1 962,327 - -0.6 962,327 - +0.7 962,394 + -0.8 962,394 + -1.7 962,394 + +0.5 962,395 - +0.1 962,395 - -0.8 962,403 + -0.3 962,434 + +0.3 962,434 + +0.7 962,434 + -1.8 962,434 + +0.2 962,435 - -0.4
Or see this region's nucleotide sequence