Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02695 and BPHYT_RS02700 are separated by 67 nucleotides BPHYT_RS02700 and BPHYT_RS02705 are separated by 265 nucleotides BPHYT_RS02705 and BPHYT_RS02710 are separated by 52 nucleotides BPHYT_RS02710 and BPHYT_RS02715 are separated by 11 nucleotides
BPHYT_RS02695: BPHYT_RS02695 - photosynthetic protein synthase I, at 605,154 to 605,771
_RS02695
BPHYT_RS02700: BPHYT_RS02700 - hypothetical protein, at 605,839 to 606,129
_RS02700
BPHYT_RS02705: BPHYT_RS02705 - membrane protein, at 606,395 to 608,269
_RS02705
BPHYT_RS02710: BPHYT_RS02710 - hypothetical protein, at 608,322 to 608,507
_RS02710
BPHYT_RS02715: BPHYT_RS02715 - dioxygenase, at 608,519 to 609,331
_RS02715
Position (kb) 606
607
608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 605.526 kb on + strand, within BPHYT_RS02695 at 605.527 kb on - strand, within BPHYT_RS02695 at 605.527 kb on - strand, within BPHYT_RS02695 at 605.650 kb on - strand, within BPHYT_RS02695 at 605.650 kb on - strand, within BPHYT_RS02695 at 605.676 kb on + strand, within BPHYT_RS02695 at 605.676 kb on + strand, within BPHYT_RS02695 at 605.677 kb on - strand, within BPHYT_RS02695 at 605.677 kb on - strand, within BPHYT_RS02695 at 606.107 kb on + strand at 606.107 kb on + strand at 606.108 kb on - strand at 606.111 kb on + strand at 606.112 kb on - strand at 606.125 kb on + strand at 606.125 kb on + strand at 606.126 kb on - strand at 606.126 kb on - strand at 606.298 kb on - strand at 606.298 kb on - strand at 606.298 kb on - strand at 606.305 kb on + strand at 606.328 kb on - strand at 606.414 kb on + strand at 606.415 kb on - strand at 606.415 kb on - strand at 606.415 kb on - strand at 606.416 kb on - strand at 606.761 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.860 kb on + strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.861 kb on - strand, within BPHYT_RS02705 at 606.935 kb on + strand, within BPHYT_RS02705 at 606.936 kb on - strand, within BPHYT_RS02705 at 607.370 kb on - strand, within BPHYT_RS02705 at 608.333 kb on - strand at 608.339 kb on - strand at 608.352 kb on - strand, within BPHYT_RS02710 at 608.499 kb on + strand at 608.717 kb on + strand, within BPHYT_RS02715 at 608.718 kb on - strand, within BPHYT_RS02715 at 608.783 kb on + strand, within BPHYT_RS02715 at 608.784 kb on - strand, within BPHYT_RS02715 at 608.813 kb on + strand, within BPHYT_RS02715 at 608.813 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.840 kb on + strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.841 kb on - strand, within BPHYT_RS02715 at 608.861 kb on + strand, within BPHYT_RS02715 at 609.011 kb on - strand, within BPHYT_RS02715 at 609.187 kb on + strand, within BPHYT_RS02715 at 609.226 kb on + strand, within BPHYT_RS02715 at 609.227 kb on - strand, within BPHYT_RS02715 at 609.264 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 605,526 + BPHYT_RS02695 0.60 -2.5 605,527 - BPHYT_RS02695 0.60 -0.0 605,527 - BPHYT_RS02695 0.60 +1.4 605,650 - BPHYT_RS02695 0.80 -0.4 605,650 - BPHYT_RS02695 0.80 +0.1 605,676 + BPHYT_RS02695 0.84 +0.1 605,676 + BPHYT_RS02695 0.84 -1.9 605,677 - BPHYT_RS02695 0.85 -0.3 605,677 - BPHYT_RS02695 0.85 +0.3 606,107 + +0.0 606,107 + -1.2 606,108 - -0.3 606,111 + +0.0 606,112 - +0.5 606,125 + +0.5 606,125 + -0.4 606,126 - +0.8 606,126 - +0.3 606,298 - +1.2 606,298 - +0.5 606,298 - -0.4 606,305 + -0.1 606,328 - +0.1 606,414 + -0.1 606,415 - -0.6 606,415 - -0.7 606,415 - +0.3 606,416 - -0.9 606,761 + BPHYT_RS02705 0.20 -0.2 606,860 + BPHYT_RS02705 0.25 +0.6 606,860 + BPHYT_RS02705 0.25 -0.6 606,860 + BPHYT_RS02705 0.25 -0.0 606,861 - BPHYT_RS02705 0.25 -3.6 606,861 - BPHYT_RS02705 0.25 -0.3 606,861 - BPHYT_RS02705 0.25 +0.9 606,935 + BPHYT_RS02705 0.29 +0.2 606,936 - BPHYT_RS02705 0.29 -0.2 607,370 - BPHYT_RS02705 0.52 -0.7 608,333 - +0.1 608,339 - +0.9 608,352 - BPHYT_RS02710 0.16 -0.5 608,499 + +0.2 608,717 + BPHYT_RS02715 0.24 +0.3 608,718 - BPHYT_RS02715 0.24 +0.4 608,783 + BPHYT_RS02715 0.32 +0.3 608,784 - BPHYT_RS02715 0.33 +0.1 608,813 + BPHYT_RS02715 0.36 +0.4 608,813 + BPHYT_RS02715 0.36 +0.4 608,840 + BPHYT_RS02715 0.39 +0.4 608,840 + BPHYT_RS02715 0.39 -0.5 608,840 + BPHYT_RS02715 0.39 -1.0 608,840 + BPHYT_RS02715 0.39 +0.8 608,840 + BPHYT_RS02715 0.39 +0.0 608,841 - BPHYT_RS02715 0.40 -1.5 608,841 - BPHYT_RS02715 0.40 +0.6 608,841 - BPHYT_RS02715 0.40 -0.2 608,841 - BPHYT_RS02715 0.40 +0.4 608,841 - BPHYT_RS02715 0.40 -0.5 608,861 + BPHYT_RS02715 0.42 +0.6 609,011 - BPHYT_RS02715 0.61 -0.4 609,187 + BPHYT_RS02715 0.82 -0.2 609,226 + BPHYT_RS02715 0.87 +0.5 609,227 - BPHYT_RS02715 0.87 +0.3 609,264 - +0.3
Or see this region's nucleotide sequence