Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02275 and BPHYT_RS02280 are separated by 15 nucleotides BPHYT_RS02280 and BPHYT_RS02285 are separated by 172 nucleotides BPHYT_RS02285 and BPHYT_RS02290 are separated by 61 nucleotides
BPHYT_RS02275: BPHYT_RS02275 - short-chain dehydrogenase, at 521,579 to 522,358
_RS02275
BPHYT_RS02280: BPHYT_RS02280 - DSBA oxidoreductase, at 522,374 to 523,012
_RS02280
BPHYT_RS02285: BPHYT_RS02285 - cell division protein, at 523,185 to 524,009
_RS02285
BPHYT_RS02290: BPHYT_RS02290 - arginine--tRNA ligase, at 524,071 to 525,861
_RS02290
Position (kb) 523
524
525 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 522.192 kb on + strand, within BPHYT_RS02275 at 522.193 kb on - strand, within BPHYT_RS02275 at 522.193 kb on - strand, within BPHYT_RS02275 at 522.311 kb on + strand at 522.311 kb on + strand at 522.312 kb on - strand at 522.312 kb on - strand at 522.375 kb on + strand at 523.029 kb on + strand at 523.030 kb on - strand at 523.030 kb on - strand at 523.136 kb on - strand at 523.171 kb on + strand at 523.171 kb on + strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.175 kb on + strand at 523.176 kb on - strand at 523.176 kb on - strand at 523.187 kb on - strand at 523.187 kb on - strand at 523.294 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.298 kb on - strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 524.072 kb on + strand at 524.072 kb on + strand at 524.072 kb on + strand at 524.073 kb on - strand at 524.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 522,192 + BPHYT_RS02275 0.79 +0.3 522,193 - BPHYT_RS02275 0.79 -0.9 522,193 - BPHYT_RS02275 0.79 -0.2 522,311 + +1.4 522,311 + -1.3 522,312 - +0.1 522,312 - -0.6 522,375 + +1.6 523,029 + -2.5 523,030 - +0.5 523,030 - +0.2 523,136 - +0.5 523,171 + +0.0 523,171 + -0.0 523,172 - +0.5 523,172 - +0.5 523,172 - +0.4 523,175 + -1.1 523,176 - -0.5 523,176 - +0.5 523,187 - +0.6 523,187 - -0.1 523,294 + BPHYT_RS02285 0.13 -2.0 523,297 + BPHYT_RS02285 0.14 +1.3 523,297 + BPHYT_RS02285 0.14 -2.0 523,297 + BPHYT_RS02285 0.14 -2.0 523,298 - BPHYT_RS02285 0.14 -2.8 523,879 + BPHYT_RS02285 0.84 -2.4 523,879 + BPHYT_RS02285 0.84 -1.9 524,072 + -2.5 524,072 + +2.7 524,072 + -1.5 524,073 - +0.6 524,073 - -0.1
Or see this region's nucleotide sequence