Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02255 and BPHYT_RS02260 are separated by 3 nucleotides BPHYT_RS02260 and BPHYT_RS02265 are separated by 243 nucleotides BPHYT_RS02265 and BPHYT_RS02270 are separated by 266 nucleotides
BPHYT_RS02255: BPHYT_RS02255 - hypothetical protein, at 517,824 to 518,288
_RS02255
BPHYT_RS02260: BPHYT_RS02260 - hypothetical protein, at 518,292 to 518,660
_RS02260
BPHYT_RS02265: BPHYT_RS02265 - peptide ABC transporter substrate-binding protein, at 518,904 to 520,490
_RS02265
BPHYT_RS02270: BPHYT_RS02270 - periplasmic binding protein, at 520,757 to 521,575
_RS02270
Position (kb) 518
519
520
521 Strain fitness (log2 ratio) -2
-1
0
1
2 at 518.003 kb on - strand, within BPHYT_RS02255 at 518.054 kb on + strand, within BPHYT_RS02255 at 518.056 kb on + strand, within BPHYT_RS02255 at 518.057 kb on - strand, within BPHYT_RS02255 at 518.091 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.101 kb on + strand, within BPHYT_RS02255 at 518.102 kb on - strand, within BPHYT_RS02255 at 518.162 kb on + strand at 518.162 kb on + strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.165 kb on - strand, within BPHYT_RS02255 at 518.265 kb on - strand at 518.359 kb on + strand, within BPHYT_RS02260 at 518.360 kb on - strand, within BPHYT_RS02260 at 518.399 kb on - strand, within BPHYT_RS02260 at 518.443 kb on - strand, within BPHYT_RS02260 at 518.479 kb on - strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.485 kb on + strand, within BPHYT_RS02260 at 518.515 kb on + strand, within BPHYT_RS02260 at 518.531 kb on - strand, within BPHYT_RS02260 at 518.646 kb on - strand at 519.185 kb on - strand, within BPHYT_RS02265 at 519.547 kb on + strand, within BPHYT_RS02265 at 519.548 kb on - strand, within BPHYT_RS02265 at 519.835 kb on + strand, within BPHYT_RS02265 at 519.857 kb on + strand, within BPHYT_RS02265 at 519.857 kb on + strand, within BPHYT_RS02265 at 519.858 kb on - strand, within BPHYT_RS02265 at 519.859 kb on + strand, within BPHYT_RS02265 at 519.860 kb on - strand, within BPHYT_RS02265 at 519.943 kb on + strand, within BPHYT_RS02265 at 519.944 kb on - strand, within BPHYT_RS02265 at 519.955 kb on + strand, within BPHYT_RS02265 at 519.955 kb on + strand, within BPHYT_RS02265 at 520.243 kb on + strand, within BPHYT_RS02265 at 520.315 kb on + strand, within BPHYT_RS02265 at 520.316 kb on - strand, within BPHYT_RS02265 at 520.461 kb on + strand at 520.461 kb on + strand at 520.462 kb on - strand at 520.540 kb on + strand at 520.599 kb on - strand at 520.599 kb on - strand at 520.665 kb on + strand at 520.695 kb on + strand at 520.785 kb on + strand at 521.060 kb on - strand, within BPHYT_RS02270 at 521.060 kb on - strand, within BPHYT_RS02270 at 521.062 kb on - strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.106 kb on + strand, within BPHYT_RS02270 at 521.223 kb on + strand, within BPHYT_RS02270 at 521.224 kb on - strand, within BPHYT_RS02270 at 521.270 kb on - strand, within BPHYT_RS02270 at 521.450 kb on + strand, within BPHYT_RS02270 at 521.450 kb on + strand, within BPHYT_RS02270
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 518,003 - BPHYT_RS02255 0.38 +0.7 518,054 + BPHYT_RS02255 0.49 +1.5 518,056 + BPHYT_RS02255 0.50 -0.1 518,057 - BPHYT_RS02255 0.50 -1.1 518,091 + BPHYT_RS02255 0.57 +0.3 518,101 + BPHYT_RS02255 0.60 -0.0 518,101 + BPHYT_RS02255 0.60 +0.4 518,102 - BPHYT_RS02255 0.60 -0.8 518,162 + +2.0 518,162 + BPHYT_RS02255 0.73 -0.8 518,165 - BPHYT_RS02255 0.73 +0.8 518,165 - BPHYT_RS02255 0.73 +0.3 518,265 - -0.1 518,359 + BPHYT_RS02260 0.18 -0.2 518,360 - BPHYT_RS02260 0.18 +0.7 518,399 - BPHYT_RS02260 0.29 -0.3 518,443 - BPHYT_RS02260 0.41 +0.2 518,479 - BPHYT_RS02260 0.51 +0.5 518,485 + BPHYT_RS02260 0.52 +0.3 518,485 + BPHYT_RS02260 0.52 +0.2 518,515 + BPHYT_RS02260 0.60 -1.0 518,531 - BPHYT_RS02260 0.65 +0.7 518,646 - +1.6 519,185 - BPHYT_RS02265 0.18 +1.4 519,547 + BPHYT_RS02265 0.41 +0.1 519,548 - BPHYT_RS02265 0.41 -0.1 519,835 + BPHYT_RS02265 0.59 +0.2 519,857 + BPHYT_RS02265 0.60 -0.7 519,857 + BPHYT_RS02265 0.60 +0.1 519,858 - BPHYT_RS02265 0.60 -0.1 519,859 + BPHYT_RS02265 0.60 -0.2 519,860 - BPHYT_RS02265 0.60 +0.1 519,943 + BPHYT_RS02265 0.65 -0.0 519,944 - BPHYT_RS02265 0.66 +0.6 519,955 + BPHYT_RS02265 0.66 +0.3 519,955 + BPHYT_RS02265 0.66 -0.2 520,243 + BPHYT_RS02265 0.84 +0.3 520,315 + BPHYT_RS02265 0.89 +0.0 520,316 - BPHYT_RS02265 0.89 -0.2 520,461 + -0.8 520,461 + -0.3 520,462 - +0.5 520,540 + -0.1 520,599 - +1.3 520,599 - -2.3 520,665 + +0.9 520,695 + +0.3 520,785 + +0.3 521,060 - BPHYT_RS02270 0.37 -0.2 521,060 - BPHYT_RS02270 0.37 -0.6 521,062 - BPHYT_RS02270 0.37 -0.3 521,106 + BPHYT_RS02270 0.43 +0.2 521,106 + BPHYT_RS02270 0.43 -0.6 521,106 + BPHYT_RS02270 0.43 +0.0 521,223 + BPHYT_RS02270 0.57 -0.0 521,224 - BPHYT_RS02270 0.57 +0.6 521,270 - BPHYT_RS02270 0.63 -0.1 521,450 + BPHYT_RS02270 0.85 -0.1 521,450 + BPHYT_RS02270 0.85 +0.5
Or see this region's nucleotide sequence