Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01955 and BPHYT_RS01960 overlap by 1 nucleotides BPHYT_RS01960 and BPHYT_RS01965 are separated by 77 nucleotides BPHYT_RS01965 and BPHYT_RS01970 are separated by 149 nucleotides BPHYT_RS01970 and BPHYT_RS01975 are separated by 248 nucleotides BPHYT_RS01975 and BPHYT_RS01980 are separated by 165 nucleotides
BPHYT_RS01955: BPHYT_RS01955 - hypothetical protein, at 442,953 to 443,750
_RS01955
BPHYT_RS01960: BPHYT_RS01960 - cytochrome C, at 443,750 to 444,097
_RS01960
BPHYT_RS01965: BPHYT_RS01965 - tRNA-Ala, at 444,175 to 444,250
_RS01965
BPHYT_RS01970: BPHYT_RS01970 - methyl-accepting chemotaxis protein, at 444,400 to 446,157
_RS01970
BPHYT_RS01975: BPHYT_RS01975 - hypothetical protein, at 446,406 to 446,639
_RS01975
BPHYT_RS01980: BPHYT_RS01980 - diguanylate cyclase, at 446,805 to 448,337
_RS01980
Position (kb) 444
445
446
447 Strain fitness (log2 ratio) -2
-1
0
1
2 at 443.446 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.936 kb on + strand, within BPHYT_RS01960 at 443.937 kb on - strand, within BPHYT_RS01960 at 444.156 kb on + strand at 444.182 kb on + strand at 444.182 kb on + strand at 444.183 kb on - strand, within BPHYT_RS01965 at 444.281 kb on - strand at 444.321 kb on + strand at 445.086 kb on + strand, within BPHYT_RS01970 at 445.086 kb on + strand, within BPHYT_RS01970 at 445.087 kb on - strand, within BPHYT_RS01970 at 445.589 kb on + strand, within BPHYT_RS01970 at 445.601 kb on + strand, within BPHYT_RS01970 at 445.602 kb on - strand, within BPHYT_RS01970 at 445.629 kb on + strand, within BPHYT_RS01970 at 445.769 kb on + strand, within BPHYT_RS01970 at 445.769 kb on + strand, within BPHYT_RS01970 at 445.770 kb on - strand, within BPHYT_RS01970 at 445.909 kb on + strand, within BPHYT_RS01970 at 445.909 kb on + strand, within BPHYT_RS01970 at 445.948 kb on - strand, within BPHYT_RS01970 at 446.055 kb on + strand at 446.095 kb on - strand at 446.239 kb on + strand at 446.264 kb on + strand at 446.264 kb on + strand at 446.264 kb on + strand at 446.383 kb on + strand at 446.383 kb on + strand at 446.383 kb on + strand at 446.384 kb on - strand at 446.384 kb on - strand at 446.437 kb on + strand, within BPHYT_RS01975 at 446.776 kb on + strand at 446.843 kb on + strand at 446.844 kb on - strand at 446.985 kb on + strand, within BPHYT_RS01980 at 446.986 kb on - strand, within BPHYT_RS01980 at 446.986 kb on - strand, within BPHYT_RS01980
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 443,446 - BPHYT_RS01955 0.62 -0.3 443,542 - BPHYT_RS01955 0.74 -2.2 443,542 - BPHYT_RS01955 0.74 +0.5 443,542 - BPHYT_RS01955 0.74 -0.6 443,936 + BPHYT_RS01960 0.53 -1.4 443,936 + BPHYT_RS01960 0.53 +0.3 443,936 + BPHYT_RS01960 0.53 +2.2 443,937 - BPHYT_RS01960 0.54 -0.4 444,156 + -0.3 444,182 + +0.4 444,182 + -1.1 444,183 - BPHYT_RS01965 0.11 -0.4 444,281 - -0.4 444,321 + -1.0 445,086 + BPHYT_RS01970 0.39 -0.6 445,086 + BPHYT_RS01970 0.39 -0.9 445,087 - BPHYT_RS01970 0.39 +0.0 445,589 + BPHYT_RS01970 0.68 +0.4 445,601 + BPHYT_RS01970 0.68 -0.7 445,602 - BPHYT_RS01970 0.68 -0.3 445,629 + BPHYT_RS01970 0.70 -0.7 445,769 + BPHYT_RS01970 0.78 -0.1 445,769 + BPHYT_RS01970 0.78 +1.0 445,770 - BPHYT_RS01970 0.78 -0.3 445,909 + BPHYT_RS01970 0.86 +0.6 445,909 + BPHYT_RS01970 0.86 -0.7 445,948 - BPHYT_RS01970 0.88 +0.8 446,055 + -0.9 446,095 - -1.1 446,239 + -0.0 446,264 + -0.4 446,264 + -1.0 446,264 + -0.2 446,383 + -0.2 446,383 + -0.7 446,383 + +0.3 446,384 - -0.3 446,384 - +0.3 446,437 + BPHYT_RS01975 0.13 -1.1 446,776 + +0.1 446,843 + +0.9 446,844 - +1.5 446,985 + BPHYT_RS01980 0.12 +0.3 446,986 - BPHYT_RS01980 0.12 +1.4 446,986 - BPHYT_RS01980 0.12 +0.6
Or see this region's nucleotide sequence