Experiment: CDM1 without Guanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16955 and BBR_RS16960 overlap by 1 nucleotides BBR_RS16960 and BBR_RS16965 are separated by 326 nucleotides BBR_RS16965 and BBR_RS16970 are separated by 10 nucleotides
BBR_RS16955: BBR_RS16955 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 1,576,322 to 1,577,374
_RS16955
BBR_RS16960: BBR_RS16960 - transcriptional regulator MraZ, at 1,577,374 to 1,577,895
_RS16960
BBR_RS16965: BBR_RS16965 - ATP-dependent DNA helicase, at 1,578,222 to 1,580,501
_RS16965
BBR_RS16970: BBR_RS16970 - phosphoglycerate dehydrogenase, at 1,580,512 to 1,581,711
_RS16970
Position (kb)
1578
1579
1580
1581 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1577.284 kb on - strand at 1577.338 kb on - strand at 1577.349 kb on - strand at 1577.372 kb on + strand at 1577.429 kb on - strand, within BBR_RS16960 at 1577.477 kb on + strand, within BBR_RS16960 at 1577.494 kb on - strand, within BBR_RS16960 at 1577.705 kb on - strand, within BBR_RS16960 at 1577.914 kb on - strand at 1577.986 kb on - strand at 1578.114 kb on + strand at 1578.119 kb on + strand at 1578.143 kb on - strand at 1578.152 kb on + strand at 1578.160 kb on - strand at 1578.179 kb on + strand at 1578.199 kb on + strand at 1578.209 kb on + strand at 1578.227 kb on + strand at 1578.227 kb on + strand at 1578.228 kb on + strand at 1578.836 kb on - strand, within BBR_RS16965 at 1578.847 kb on - strand, within BBR_RS16965 at 1578.995 kb on - strand, within BBR_RS16965 at 1579.012 kb on - strand, within BBR_RS16965 at 1579.136 kb on + strand, within BBR_RS16965 at 1579.529 kb on - strand, within BBR_RS16965 at 1579.529 kb on - strand, within BBR_RS16965 at 1579.564 kb on - strand, within BBR_RS16965 at 1579.568 kb on + strand, within BBR_RS16965 at 1580.013 kb on - strand, within BBR_RS16965 at 1580.475 kb on - strand at 1580.495 kb on + strand at 1580.503 kb on - strand at 1580.602 kb on + strand at 1580.620 kb on - strand at 1580.625 kb on - strand at 1580.638 kb on + strand, within BBR_RS16970 at 1580.638 kb on - strand, within BBR_RS16970 at 1580.695 kb on - strand, within BBR_RS16970 at 1580.715 kb on - strand, within BBR_RS16970 at 1580.749 kb on + strand, within BBR_RS16970 at 1580.784 kb on + strand, within BBR_RS16970 at 1580.784 kb on + strand, within BBR_RS16970 at 1580.813 kb on - strand, within BBR_RS16970 at 1580.840 kb on + strand, within BBR_RS16970 at 1580.840 kb on + strand, within BBR_RS16970 at 1580.880 kb on + strand, within BBR_RS16970 at 1580.918 kb on + strand, within BBR_RS16970 at 1580.920 kb on + strand, within BBR_RS16970 at 1580.926 kb on - strand, within BBR_RS16970 at 1580.995 kb on + strand, within BBR_RS16970 at 1581.008 kb on - strand, within BBR_RS16970 at 1581.013 kb on + strand, within BBR_RS16970 at 1581.035 kb on + strand, within BBR_RS16970 at 1581.035 kb on + strand, within BBR_RS16970 at 1581.043 kb on - strand, within BBR_RS16970 at 1581.067 kb on + strand, within BBR_RS16970 at 1581.067 kb on + strand, within BBR_RS16970 at 1581.067 kb on + strand, within BBR_RS16970 at 1581.080 kb on + strand, within BBR_RS16970 at 1581.088 kb on - strand, within BBR_RS16970 at 1581.090 kb on + strand, within BBR_RS16970 at 1581.098 kb on - strand, within BBR_RS16970 at 1581.098 kb on - strand, within BBR_RS16970 at 1581.190 kb on + strand, within BBR_RS16970 at 1581.195 kb on + strand, within BBR_RS16970 at 1581.250 kb on + strand, within BBR_RS16970 at 1581.255 kb on + strand, within BBR_RS16970 at 1581.266 kb on + strand, within BBR_RS16970 at 1581.379 kb on + strand, within BBR_RS16970 at 1581.379 kb on + strand, within BBR_RS16970 at 1581.379 kb on + strand, within BBR_RS16970 at 1581.387 kb on - strand, within BBR_RS16970 at 1581.490 kb on + strand, within BBR_RS16970 at 1581.490 kb on + strand, within BBR_RS16970 at 1581.498 kb on - strand, within BBR_RS16970
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Guanine remove 1,577,284 - -1.4 1,577,338 - -2.1 1,577,349 - -0.9 1,577,372 + -1.5 1,577,429 - BBR_RS16960 0.11 -0.4 1,577,477 + BBR_RS16960 0.20 -3.7 1,577,494 - BBR_RS16960 0.23 +0.1 1,577,705 - BBR_RS16960 0.63 +0.1 1,577,914 - +0.3 1,577,986 - -0.1 1,578,114 + -0.9 1,578,119 + +0.1 1,578,143 - +0.0 1,578,152 + +0.4 1,578,160 - -1.3 1,578,179 + -0.9 1,578,199 + -0.7 1,578,209 + -1.8 1,578,227 + +0.8 1,578,227 + +1.0 1,578,228 + -1.8 1,578,836 - BBR_RS16965 0.27 +0.0 1,578,847 - BBR_RS16965 0.27 -1.8 1,578,995 - BBR_RS16965 0.34 -2.5 1,579,012 - BBR_RS16965 0.35 -1.4 1,579,136 + BBR_RS16965 0.40 -2.0 1,579,529 - BBR_RS16965 0.57 -2.3 1,579,529 - BBR_RS16965 0.57 -0.7 1,579,564 - BBR_RS16965 0.59 -0.8 1,579,568 + BBR_RS16965 0.59 -0.5 1,580,013 - BBR_RS16965 0.79 -1.5 1,580,475 - -2.2 1,580,495 + +0.7 1,580,503 - -3.2 1,580,602 + -2.2 1,580,620 - -3.0 1,580,625 - -4.1 1,580,638 + BBR_RS16970 0.10 -2.9 1,580,638 - BBR_RS16970 0.10 -1.8 1,580,695 - BBR_RS16970 0.15 -1.3 1,580,715 - BBR_RS16970 0.17 -2.5 1,580,749 + BBR_RS16970 0.20 -3.8 1,580,784 + BBR_RS16970 0.23 -2.6 1,580,784 + BBR_RS16970 0.23 -2.2 1,580,813 - BBR_RS16970 0.25 -2.6 1,580,840 + BBR_RS16970 0.27 -3.4 1,580,840 + BBR_RS16970 0.27 -2.9 1,580,880 + BBR_RS16970 0.31 -2.1 1,580,918 + BBR_RS16970 0.34 -2.4 1,580,920 + BBR_RS16970 0.34 -1.1 1,580,926 - BBR_RS16970 0.34 -1.4 1,580,995 + BBR_RS16970 0.40 -3.3 1,581,008 - BBR_RS16970 0.41 -4.0 1,581,013 + BBR_RS16970 0.42 -1.7 1,581,035 + BBR_RS16970 0.44 -2.9 1,581,035 + BBR_RS16970 0.44 -2.5 1,581,043 - BBR_RS16970 0.44 -3.9 1,581,067 + BBR_RS16970 0.46 -1.8 1,581,067 + BBR_RS16970 0.46 -3.1 1,581,067 + BBR_RS16970 0.46 -3.2 1,581,080 + BBR_RS16970 0.47 -1.8 1,581,088 - BBR_RS16970 0.48 +0.6 1,581,090 + BBR_RS16970 0.48 -2.4 1,581,098 - BBR_RS16970 0.49 -0.8 1,581,098 - BBR_RS16970 0.49 -3.6 1,581,190 + BBR_RS16970 0.56 -2.3 1,581,195 + BBR_RS16970 0.57 -1.5 1,581,250 + BBR_RS16970 0.61 -3.0 1,581,255 + BBR_RS16970 0.62 -1.5 1,581,266 + BBR_RS16970 0.63 -4.1 1,581,379 + BBR_RS16970 0.72 -2.0 1,581,379 + BBR_RS16970 0.72 -3.7 1,581,379 + BBR_RS16970 0.72 -2.8 1,581,387 - BBR_RS16970 0.73 -4.0 1,581,490 + BBR_RS16970 0.81 -3.5 1,581,490 + BBR_RS16970 0.81 -1.9 1,581,498 - BBR_RS16970 0.82 -3.1
Or see this region's nucleotide sequence