Experiment: CDM1 without Guanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15825 and BBR_RS15830 are separated by 132 nucleotides BBR_RS15830 and BBR_RS20925 are separated by 5 nucleotides BBR_RS20925 and BBR_RS15835 are separated by 201 nucleotides
BBR_RS15825: BBR_RS15825 - deoxyribonuclease IV, at 1,315,849 to 1,316,703
_RS15825
BBR_RS15830: BBR_RS15830 - amino acid permease, at 1,316,836 to 1,318,236
_RS15830
BBR_RS20925: BBR_RS20925 - amino acid permease, at 1,318,242 to 1,318,397
_RS20925
BBR_RS15835: BBR_RS15835 - MFS transporter, at 1,318,599 to 1,319,207
_RS15835
Position (kb)
1316
1317
1318
1319 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1315.857 kb on + strand at 1315.928 kb on + strand at 1315.935 kb on - strand, within BBR_RS15825 at 1315.947 kb on - strand, within BBR_RS15825 at 1315.948 kb on - strand, within BBR_RS15825 at 1316.002 kb on - strand, within BBR_RS15825 at 1316.002 kb on - strand, within BBR_RS15825 at 1316.025 kb on + strand, within BBR_RS15825 at 1316.038 kb on - strand, within BBR_RS15825 at 1316.060 kb on + strand, within BBR_RS15825 at 1316.066 kb on + strand, within BBR_RS15825 at 1316.068 kb on - strand, within BBR_RS15825 at 1316.068 kb on - strand, within BBR_RS15825 at 1316.068 kb on - strand, within BBR_RS15825 at 1316.078 kb on - strand, within BBR_RS15825 at 1316.188 kb on - strand, within BBR_RS15825 at 1316.271 kb on + strand, within BBR_RS15825 at 1316.271 kb on + strand, within BBR_RS15825 at 1316.279 kb on - strand, within BBR_RS15825 at 1316.279 kb on - strand, within BBR_RS15825 at 1316.302 kb on + strand, within BBR_RS15825 at 1316.310 kb on - strand, within BBR_RS15825 at 1316.341 kb on + strand, within BBR_RS15825 at 1316.347 kb on + strand, within BBR_RS15825 at 1316.349 kb on - strand, within BBR_RS15825 at 1316.355 kb on - strand, within BBR_RS15825 at 1316.382 kb on + strand, within BBR_RS15825 at 1316.382 kb on + strand, within BBR_RS15825 at 1316.405 kb on + strand, within BBR_RS15825 at 1316.423 kb on + strand, within BBR_RS15825 at 1316.436 kb on - strand, within BBR_RS15825 at 1316.453 kb on + strand, within BBR_RS15825 at 1316.459 kb on + strand, within BBR_RS15825 at 1316.459 kb on + strand, within BBR_RS15825 at 1316.467 kb on - strand, within BBR_RS15825 at 1316.525 kb on + strand, within BBR_RS15825 at 1316.533 kb on - strand, within BBR_RS15825 at 1316.533 kb on - strand, within BBR_RS15825 at 1316.533 kb on - strand, within BBR_RS15825 at 1316.570 kb on + strand, within BBR_RS15825 at 1316.571 kb on - strand, within BBR_RS15825 at 1316.634 kb on - strand at 1316.636 kb on - strand at 1316.636 kb on - strand at 1316.636 kb on - strand at 1316.636 kb on - strand at 1316.698 kb on - strand at 1316.725 kb on - strand at 1316.795 kb on - strand at 1316.803 kb on - strand at 1316.803 kb on - strand at 1316.804 kb on - strand at 1316.804 kb on - strand at 1316.804 kb on - strand at 1316.829 kb on + strand at 1316.859 kb on + strand at 1316.938 kb on - strand at 1316.970 kb on + strand at 1316.978 kb on - strand, within BBR_RS15830 at 1317.028 kb on + strand, within BBR_RS15830 at 1317.051 kb on + strand, within BBR_RS15830 at 1317.051 kb on + strand, within BBR_RS15830 at 1317.058 kb on + strand, within BBR_RS15830 at 1317.079 kb on + strand, within BBR_RS15830 at 1317.094 kb on + strand, within BBR_RS15830 at 1317.118 kb on + strand, within BBR_RS15830 at 1317.118 kb on + strand, within BBR_RS15830 at 1317.136 kb on - strand, within BBR_RS15830 at 1317.174 kb on + strand, within BBR_RS15830 at 1317.278 kb on - strand, within BBR_RS15830 at 1317.293 kb on - strand, within BBR_RS15830 at 1317.293 kb on - strand, within BBR_RS15830 at 1317.311 kb on - strand, within BBR_RS15830 at 1317.311 kb on - strand, within BBR_RS15830 at 1317.369 kb on + strand, within BBR_RS15830 at 1317.374 kb on + strand, within BBR_RS15830 at 1317.570 kb on - strand, within BBR_RS15830 at 1317.650 kb on + strand, within BBR_RS15830 at 1317.737 kb on - strand, within BBR_RS15830 at 1317.749 kb on - strand, within BBR_RS15830 at 1317.763 kb on + strand, within BBR_RS15830 at 1317.852 kb on + strand, within BBR_RS15830 at 1317.860 kb on - strand, within BBR_RS15830 at 1317.862 kb on + strand, within BBR_RS15830 at 1317.862 kb on + strand, within BBR_RS15830 at 1317.865 kb on + strand, within BBR_RS15830 at 1317.865 kb on - strand, within BBR_RS15830 at 1317.865 kb on - strand, within BBR_RS15830 at 1317.875 kb on - strand, within BBR_RS15830 at 1317.876 kb on + strand, within BBR_RS15830 at 1317.934 kb on - strand, within BBR_RS15830 at 1317.970 kb on + strand, within BBR_RS15830 at 1317.970 kb on - strand, within BBR_RS15830 at 1317.972 kb on + strand, within BBR_RS15830 at 1318.072 kb on - strand, within BBR_RS15830 at 1318.077 kb on + strand, within BBR_RS15830 at 1318.077 kb on + strand, within BBR_RS15830 at 1318.083 kb on - strand, within BBR_RS15830 at 1318.142 kb on + strand at 1318.142 kb on + strand at 1318.147 kb on + strand at 1318.219 kb on + strand at 1318.305 kb on + strand, within BBR_RS20925 at 1318.317 kb on + strand, within BBR_RS20925 at 1318.356 kb on - strand, within BBR_RS20925 at 1318.534 kb on - strand at 1318.542 kb on - strand at 1318.542 kb on - strand at 1318.552 kb on - strand at 1318.554 kb on - strand at 1318.556 kb on + strand at 1318.566 kb on + strand at 1318.659 kb on + strand at 1318.667 kb on - strand, within BBR_RS15835 at 1318.669 kb on - strand, within BBR_RS15835 at 1318.743 kb on - strand, within BBR_RS15835 at 1318.745 kb on + strand, within BBR_RS15835 at 1318.753 kb on - strand, within BBR_RS15835 at 1318.770 kb on + strand, within BBR_RS15835 at 1318.861 kb on + strand, within BBR_RS15835 at 1318.889 kb on - strand, within BBR_RS15835 at 1318.994 kb on + strand, within BBR_RS15835 at 1319.002 kb on - strand, within BBR_RS15835 at 1319.013 kb on + strand, within BBR_RS15835 at 1319.070 kb on + strand, within BBR_RS15835 at 1319.085 kb on - strand, within BBR_RS15835 at 1319.091 kb on - strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.112 kb on + strand, within BBR_RS15835 at 1319.117 kb on + strand, within BBR_RS15835 at 1319.120 kb on - strand, within BBR_RS15835 at 1319.120 kb on - strand, within BBR_RS15835 at 1319.129 kb on + strand, within BBR_RS15835 at 1319.150 kb on + strand at 1319.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Guanine remove 1,315,857 + -0.3 1,315,928 + -0.8 1,315,935 - BBR_RS15825 0.10 +0.2 1,315,947 - BBR_RS15825 0.11 -0.6 1,315,948 - BBR_RS15825 0.12 +0.6 1,316,002 - BBR_RS15825 0.18 +0.9 1,316,002 - BBR_RS15825 0.18 +0.6 1,316,025 + BBR_RS15825 0.21 -1.1 1,316,038 - BBR_RS15825 0.22 -1.1 1,316,060 + BBR_RS15825 0.25 -0.8 1,316,066 + BBR_RS15825 0.25 +0.8 1,316,068 - BBR_RS15825 0.26 -0.6 1,316,068 - BBR_RS15825 0.26 -0.1 1,316,068 - BBR_RS15825 0.26 -0.5 1,316,078 - BBR_RS15825 0.27 -0.8 1,316,188 - BBR_RS15825 0.40 +0.8 1,316,271 + BBR_RS15825 0.49 -3.0 1,316,271 + BBR_RS15825 0.49 -0.3 1,316,279 - BBR_RS15825 0.50 +0.4 1,316,279 - BBR_RS15825 0.50 +0.5 1,316,302 + BBR_RS15825 0.53 -0.7 1,316,310 - BBR_RS15825 0.54 -0.7 1,316,341 + BBR_RS15825 0.58 -0.9 1,316,347 + BBR_RS15825 0.58 +0.3 1,316,349 - BBR_RS15825 0.58 +0.7 1,316,355 - BBR_RS15825 0.59 -0.0 1,316,382 + BBR_RS15825 0.62 +0.0 1,316,382 + BBR_RS15825 0.62 -2.4 1,316,405 + BBR_RS15825 0.65 -0.6 1,316,423 + BBR_RS15825 0.67 -1.7 1,316,436 - BBR_RS15825 0.69 -0.6 1,316,453 + BBR_RS15825 0.71 -0.2 1,316,459 + BBR_RS15825 0.71 -1.5 1,316,459 + BBR_RS15825 0.71 +0.2 1,316,467 - BBR_RS15825 0.72 -1.5 1,316,525 + BBR_RS15825 0.79 -1.8 1,316,533 - BBR_RS15825 0.80 -0.6 1,316,533 - BBR_RS15825 0.80 -0.8 1,316,533 - BBR_RS15825 0.80 -1.1 1,316,570 + BBR_RS15825 0.84 -0.4 1,316,571 - BBR_RS15825 0.84 -1.0 1,316,634 - +0.7 1,316,636 - -1.9 1,316,636 - -0.3 1,316,636 - +0.8 1,316,636 - +0.8 1,316,698 - -1.8 1,316,725 - +1.6 1,316,795 - -1.7 1,316,803 - +0.2 1,316,803 - +0.3 1,316,804 - -0.7 1,316,804 - +0.0 1,316,804 - -4.1 1,316,829 + -0.5 1,316,859 + -0.2 1,316,938 - -0.9 1,316,970 + +1.2 1,316,978 - BBR_RS15830 0.10 +1.8 1,317,028 + BBR_RS15830 0.14 -1.3 1,317,051 + BBR_RS15830 0.15 +0.6 1,317,051 + BBR_RS15830 0.15 +1.6 1,317,058 + BBR_RS15830 0.16 -0.5 1,317,079 + BBR_RS15830 0.17 -0.2 1,317,094 + BBR_RS15830 0.18 +0.6 1,317,118 + BBR_RS15830 0.20 -0.3 1,317,118 + BBR_RS15830 0.20 +0.2 1,317,136 - BBR_RS15830 0.21 +0.5 1,317,174 + BBR_RS15830 0.24 +0.3 1,317,278 - BBR_RS15830 0.32 -1.9 1,317,293 - BBR_RS15830 0.33 +1.1 1,317,293 - BBR_RS15830 0.33 -0.6 1,317,311 - BBR_RS15830 0.34 +1.2 1,317,311 - BBR_RS15830 0.34 +0.7 1,317,369 + BBR_RS15830 0.38 +0.2 1,317,374 + BBR_RS15830 0.38 +0.2 1,317,570 - BBR_RS15830 0.52 -1.5 1,317,650 + BBR_RS15830 0.58 +1.7 1,317,737 - BBR_RS15830 0.64 +1.9 1,317,749 - BBR_RS15830 0.65 +1.4 1,317,763 + BBR_RS15830 0.66 +1.7 1,317,852 + BBR_RS15830 0.73 -0.8 1,317,860 - BBR_RS15830 0.73 +0.2 1,317,862 + BBR_RS15830 0.73 +0.6 1,317,862 + BBR_RS15830 0.73 +0.4 1,317,865 + BBR_RS15830 0.73 +1.5 1,317,865 - BBR_RS15830 0.73 -0.8 1,317,865 - BBR_RS15830 0.73 -2.2 1,317,875 - BBR_RS15830 0.74 -1.1 1,317,876 + BBR_RS15830 0.74 +0.5 1,317,934 - BBR_RS15830 0.78 +1.6 1,317,970 + BBR_RS15830 0.81 -1.0 1,317,970 - BBR_RS15830 0.81 -0.3 1,317,972 + BBR_RS15830 0.81 +0.1 1,318,072 - BBR_RS15830 0.88 +0.7 1,318,077 + BBR_RS15830 0.89 +1.0 1,318,077 + BBR_RS15830 0.89 -0.6 1,318,083 - BBR_RS15830 0.89 +0.7 1,318,142 + -0.4 1,318,142 + +0.6 1,318,147 + +0.7 1,318,219 + +0.5 1,318,305 + BBR_RS20925 0.40 -3.1 1,318,317 + BBR_RS20925 0.48 -2.5 1,318,356 - BBR_RS20925 0.73 +0.9 1,318,534 - +1.7 1,318,542 - +0.4 1,318,542 - -0.1 1,318,552 - -0.7 1,318,554 - +0.5 1,318,556 + +0.6 1,318,566 + +0.3 1,318,659 + +0.5 1,318,667 - BBR_RS15835 0.11 +2.1 1,318,669 - BBR_RS15835 0.11 -2.1 1,318,743 - BBR_RS15835 0.24 -0.5 1,318,745 + BBR_RS15835 0.24 -1.0 1,318,753 - BBR_RS15835 0.25 -3.0 1,318,770 + BBR_RS15835 0.28 +0.3 1,318,861 + BBR_RS15835 0.43 -1.1 1,318,889 - BBR_RS15835 0.48 -2.0 1,318,994 + BBR_RS15835 0.65 -0.6 1,319,002 - BBR_RS15835 0.66 -0.3 1,319,013 + BBR_RS15835 0.68 +0.9 1,319,070 + BBR_RS15835 0.77 +0.9 1,319,085 - BBR_RS15835 0.80 -0.4 1,319,091 - BBR_RS15835 0.81 +0.3 1,319,112 + BBR_RS15835 0.84 -0.6 1,319,112 + BBR_RS15835 0.84 +0.2 1,319,112 + BBR_RS15835 0.84 +0.6 1,319,112 + BBR_RS15835 0.84 -0.6 1,319,112 + BBR_RS15835 0.84 -0.6 1,319,112 + BBR_RS15835 0.84 +0.5 1,319,117 + BBR_RS15835 0.85 +0.4 1,319,120 - BBR_RS15835 0.86 +0.5 1,319,120 - BBR_RS15835 0.86 -0.9 1,319,129 + BBR_RS15835 0.87 -0.5 1,319,150 + +0.5 1,319,224 - -0.8
Or see this region's nucleotide sequence