Experiment: CDM1 without Guanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15685 and BBR_RS15690 overlap by 53 nucleotides BBR_RS15690 and BBR_RS20920 are separated by 154 nucleotides BBR_RS20920 and BBR_RS15695 overlap by 102 nucleotides
BBR_RS15685: BBR_RS15685 - hypothetical protein, at 1,287,549 to 1,288,934
_RS15685
BBR_RS15690: BBR_RS15690 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH, at 1,288,882 to 1,290,519
_RS15690
BBR_RS20920: BBR_RS20920 - hypothetical protein, at 1,290,674 to 1,290,976
_RS20920
BBR_RS15695: BBR_RS15695 - membrane channel protein, at 1,290,875 to 1,291,813
_RS15695
Position (kb)
1288
1289
1290
1291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1287.922 kb on - strand, within BBR_RS15685 at 1287.927 kb on - strand, within BBR_RS15685 at 1287.953 kb on + strand, within BBR_RS15685 at 1287.961 kb on - strand, within BBR_RS15685 at 1287.997 kb on + strand, within BBR_RS15685 at 1288.005 kb on + strand, within BBR_RS15685 at 1288.007 kb on + strand, within BBR_RS15685 at 1288.007 kb on + strand, within BBR_RS15685 at 1288.015 kb on - strand, within BBR_RS15685 at 1288.015 kb on - strand, within BBR_RS15685 at 1288.024 kb on - strand, within BBR_RS15685 at 1288.069 kb on - strand, within BBR_RS15685 at 1288.111 kb on + strand, within BBR_RS15685 at 1288.119 kb on - strand, within BBR_RS15685 at 1288.174 kb on - strand, within BBR_RS15685 at 1288.236 kb on - strand, within BBR_RS15685 at 1288.281 kb on - strand, within BBR_RS15685 at 1288.288 kb on + strand, within BBR_RS15685 at 1288.301 kb on - strand, within BBR_RS15685 at 1288.326 kb on - strand, within BBR_RS15685 at 1288.330 kb on + strand, within BBR_RS15685 at 1288.330 kb on + strand, within BBR_RS15685 at 1288.335 kb on + strand, within BBR_RS15685 at 1288.335 kb on + strand, within BBR_RS15685 at 1288.338 kb on - strand, within BBR_RS15685 at 1288.340 kb on + strand, within BBR_RS15685 at 1288.340 kb on + strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.348 kb on - strand, within BBR_RS15685 at 1288.367 kb on - strand, within BBR_RS15685 at 1288.387 kb on - strand, within BBR_RS15685 at 1288.387 kb on - strand, within BBR_RS15685 at 1288.393 kb on - strand, within BBR_RS15685 at 1288.401 kb on + strand, within BBR_RS15685 at 1288.421 kb on - strand, within BBR_RS15685 at 1288.470 kb on - strand, within BBR_RS15685 at 1288.470 kb on - strand, within BBR_RS15685 at 1288.494 kb on + strand, within BBR_RS15685 at 1288.507 kb on - strand, within BBR_RS15685 at 1288.507 kb on - strand, within BBR_RS15685 at 1288.539 kb on + strand, within BBR_RS15685 at 1288.612 kb on - strand, within BBR_RS15685 at 1288.657 kb on + strand, within BBR_RS15685 at 1288.700 kb on + strand, within BBR_RS15685 at 1288.708 kb on - strand, within BBR_RS15685 at 1288.823 kb on + strand at 1288.842 kb on - strand at 1290.643 kb on - strand at 1290.643 kb on - strand at 1290.677 kb on - strand at 1290.709 kb on + strand, within BBR_RS20920 at 1290.710 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.715 kb on + strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.723 kb on - strand, within BBR_RS20920 at 1290.729 kb on + strand, within BBR_RS20920 at 1290.763 kb on + strand, within BBR_RS20920 at 1290.775 kb on + strand, within BBR_RS20920 at 1290.861 kb on - strand, within BBR_RS20920 at 1290.965 kb on + strand at 1290.972 kb on + strand at 1290.992 kb on - strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.041 kb on + strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.127 kb on - strand, within BBR_RS15695 at 1291.198 kb on - strand, within BBR_RS15695 at 1291.250 kb on + strand, within BBR_RS15695 at 1291.258 kb on - strand, within BBR_RS15695 at 1291.373 kb on - strand, within BBR_RS15695 at 1291.379 kb on - strand, within BBR_RS15695 at 1291.446 kb on + strand, within BBR_RS15695 at 1291.510 kb on - strand, within BBR_RS15695
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Guanine remove 1,287,922 - BBR_RS15685 0.27 -1.2 1,287,927 - BBR_RS15685 0.27 +0.1 1,287,953 + BBR_RS15685 0.29 +0.9 1,287,961 - BBR_RS15685 0.30 +1.0 1,287,997 + BBR_RS15685 0.32 -0.2 1,288,005 + BBR_RS15685 0.33 +0.4 1,288,007 + BBR_RS15685 0.33 +1.0 1,288,007 + BBR_RS15685 0.33 -0.3 1,288,015 - BBR_RS15685 0.34 -0.2 1,288,015 - BBR_RS15685 0.34 -0.6 1,288,024 - BBR_RS15685 0.34 -1.5 1,288,069 - BBR_RS15685 0.38 -2.1 1,288,111 + BBR_RS15685 0.41 +0.9 1,288,119 - BBR_RS15685 0.41 -0.9 1,288,174 - BBR_RS15685 0.45 -1.1 1,288,236 - BBR_RS15685 0.50 -2.1 1,288,281 - BBR_RS15685 0.53 -0.0 1,288,288 + BBR_RS15685 0.53 -0.4 1,288,301 - BBR_RS15685 0.54 -1.3 1,288,326 - BBR_RS15685 0.56 -0.1 1,288,330 + BBR_RS15685 0.56 -0.4 1,288,330 + BBR_RS15685 0.56 +0.5 1,288,335 + BBR_RS15685 0.57 -0.6 1,288,335 + BBR_RS15685 0.57 +1.1 1,288,338 - BBR_RS15685 0.57 +0.5 1,288,340 + BBR_RS15685 0.57 +0.8 1,288,340 + BBR_RS15685 0.57 -2.4 1,288,348 - BBR_RS15685 0.58 -0.6 1,288,348 - BBR_RS15685 0.58 -1.1 1,288,348 - BBR_RS15685 0.58 +1.1 1,288,348 - BBR_RS15685 0.58 -0.4 1,288,348 - BBR_RS15685 0.58 +0.1 1,288,348 - BBR_RS15685 0.58 -0.6 1,288,348 - BBR_RS15685 0.58 -0.5 1,288,367 - BBR_RS15685 0.59 -1.7 1,288,387 - BBR_RS15685 0.60 -0.9 1,288,387 - BBR_RS15685 0.60 -1.4 1,288,393 - BBR_RS15685 0.61 -0.6 1,288,401 + BBR_RS15685 0.61 -0.0 1,288,421 - BBR_RS15685 0.63 +0.7 1,288,470 - BBR_RS15685 0.66 +0.0 1,288,470 - BBR_RS15685 0.66 -1.0 1,288,494 + BBR_RS15685 0.68 +0.7 1,288,507 - BBR_RS15685 0.69 -1.6 1,288,507 - BBR_RS15685 0.69 -0.2 1,288,539 + BBR_RS15685 0.71 -1.5 1,288,612 - BBR_RS15685 0.77 +0.3 1,288,657 + BBR_RS15685 0.80 +0.1 1,288,700 + BBR_RS15685 0.83 +1.1 1,288,708 - BBR_RS15685 0.84 -0.7 1,288,823 + +0.1 1,288,842 - -1.8 1,290,643 - +0.1 1,290,643 - +0.3 1,290,677 - -1.8 1,290,709 + BBR_RS20920 0.12 -4.3 1,290,710 + BBR_RS20920 0.12 -2.1 1,290,715 + BBR_RS20920 0.14 -0.4 1,290,715 + BBR_RS20920 0.14 -2.5 1,290,723 - BBR_RS20920 0.16 -0.7 1,290,723 - BBR_RS20920 0.16 -0.9 1,290,729 + BBR_RS20920 0.18 -2.1 1,290,763 + BBR_RS20920 0.29 -1.1 1,290,775 + BBR_RS20920 0.33 -1.6 1,290,861 - BBR_RS20920 0.62 -2.6 1,290,965 + -1.8 1,290,972 + +1.1 1,290,992 - BBR_RS15695 0.12 +0.6 1,291,041 + BBR_RS15695 0.18 -0.7 1,291,041 + BBR_RS15695 0.18 -2.5 1,291,041 + BBR_RS15695 0.18 +0.4 1,291,041 + BBR_RS15695 0.18 +0.2 1,291,041 + BBR_RS15695 0.18 -0.3 1,291,041 + BBR_RS15695 0.18 +0.8 1,291,041 + BBR_RS15695 0.18 -0.2 1,291,127 - BBR_RS15695 0.27 +0.3 1,291,127 - BBR_RS15695 0.27 -0.2 1,291,198 - BBR_RS15695 0.34 +0.0 1,291,250 + BBR_RS15695 0.40 -1.2 1,291,258 - BBR_RS15695 0.41 +0.6 1,291,373 - BBR_RS15695 0.53 +0.0 1,291,379 - BBR_RS15695 0.54 +0.2 1,291,446 + BBR_RS15695 0.61 -1.6 1,291,510 - BBR_RS15695 0.68 -0.3
Or see this region's nucleotide sequence