Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS31530

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS31520 and RR42_RS31525 are separated by 125 nucleotidesRR42_RS31525 and RR42_RS31530 overlap by 20 nucleotidesRR42_RS31530 and RR42_RS31535 overlap by 1 nucleotidesRR42_RS31535 and RR42_RS31540 are separated by 218 nucleotides RR42_RS31520: RR42_RS31520 - 2-nitropropane dioxygenase, at 2,449,716 to 2,450,834 _RS31520 RR42_RS31525: RR42_RS31525 - threonine transporter, at 2,450,960 to 2,451,601 _RS31525 RR42_RS31530: RR42_RS31530 - histidine kinase, at 2,451,582 to 2,453,042 _RS31530 RR42_RS31535: RR42_RS31535 - transcriptional regulator, at 2,453,042 to 2,453,716 _RS31535 RR42_RS31540: RR42_RS31540 - RND transporter, at 2,453,935 to 2,457,180 _RS31540 Position (kb) 2451 2452 2453 2454Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2450.646 kb on + strand, within RR42_RS31520at 2450.647 kb on - strand, within RR42_RS31520at 2450.647 kb on - strand, within RR42_RS31520at 2450.700 kb on + strand, within RR42_RS31520at 2450.875 kb on + strandat 2450.875 kb on + strandat 2450.876 kb on - strandat 2450.876 kb on - strandat 2450.888 kb on + strandat 2450.888 kb on + strandat 2450.889 kb on - strandat 2450.889 kb on - strandat 2450.889 kb on - strandat 2451.083 kb on + strand, within RR42_RS31525at 2451.083 kb on + strand, within RR42_RS31525at 2451.084 kb on - strand, within RR42_RS31525at 2451.084 kb on - strand, within RR42_RS31525at 2451.176 kb on + strand, within RR42_RS31525at 2451.176 kb on + strand, within RR42_RS31525at 2451.176 kb on + strand, within RR42_RS31525at 2451.176 kb on + strand, within RR42_RS31525at 2451.177 kb on - strand, within RR42_RS31525at 2451.206 kb on + strand, within RR42_RS31525at 2451.206 kb on + strand, within RR42_RS31525at 2451.207 kb on - strand, within RR42_RS31525at 2451.207 kb on - strand, within RR42_RS31525at 2451.215 kb on + strand, within RR42_RS31525at 2451.216 kb on - strand, within RR42_RS31525at 2451.216 kb on - strand, within RR42_RS31525at 2451.369 kb on - strand, within RR42_RS31525at 2451.513 kb on - strand, within RR42_RS31525at 2451.555 kb on + strandat 2451.556 kb on - strandat 2451.599 kb on + strandat 2451.599 kb on + strandat 2452.475 kb on - strand, within RR42_RS31530at 2452.619 kb on + strand, within RR42_RS31530at 2452.960 kb on + strandat 2452.961 kb on - strandat 2453.583 kb on + strand, within RR42_RS31535at 2453.584 kb on - strand, within RR42_RS31535at 2453.584 kb on - strand, within RR42_RS31535at 2453.584 kb on - strand, within RR42_RS31535at 2453.674 kb on - strandat 2453.811 kb on + strandat 2453.853 kb on + strandat 2453.853 kb on + strandat 2453.854 kb on - strandat 2453.854 kb on - strandat 2453.968 kb on + strandat 2454.039 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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2,450,646 + RR42_RS31520 0.83 -2.5
2,450,647 - RR42_RS31520 0.83 -0.2
2,450,647 - RR42_RS31520 0.83 -0.2
2,450,700 + RR42_RS31520 0.88 +3.5
2,450,875 + -0.9
2,450,875 + +0.3
2,450,876 - +0.1
2,450,876 - -0.2
2,450,888 + -0.4
2,450,888 + -0.4
2,450,889 - -0.4
2,450,889 - +0.3
2,450,889 - +0.5
2,451,083 + RR42_RS31525 0.19 -0.6
2,451,083 + RR42_RS31525 0.19 -0.5
2,451,084 - RR42_RS31525 0.19 -0.4
2,451,084 - RR42_RS31525 0.19 +0.2
2,451,176 + RR42_RS31525 0.34 -0.8
2,451,176 + RR42_RS31525 0.34 -0.6
2,451,176 + RR42_RS31525 0.34 -0.3
2,451,176 + RR42_RS31525 0.34 -0.3
2,451,177 - RR42_RS31525 0.34 -0.6
2,451,206 + RR42_RS31525 0.38 +0.4
2,451,206 + RR42_RS31525 0.38 -0.5
2,451,207 - RR42_RS31525 0.38 -0.0
2,451,207 - RR42_RS31525 0.38 -0.5
2,451,215 + RR42_RS31525 0.40 -0.0
2,451,216 - RR42_RS31525 0.40 -0.2
2,451,216 - RR42_RS31525 0.40 -0.5
2,451,369 - RR42_RS31525 0.64 -3.7
2,451,513 - RR42_RS31525 0.86 +0.0
2,451,555 + -0.3
2,451,556 - +0.2
2,451,599 + +0.5
2,451,599 + -0.9
2,452,475 - RR42_RS31530 0.61 -1.5
2,452,619 + RR42_RS31530 0.71 -0.8
2,452,960 + +0.2
2,452,961 - -0.8
2,453,583 + RR42_RS31535 0.80 -0.5
2,453,584 - RR42_RS31535 0.80 -0.4
2,453,584 - RR42_RS31535 0.80 -0.1
2,453,584 - RR42_RS31535 0.80 +0.0
2,453,674 - -0.2
2,453,811 + +0.1
2,453,853 + -0.4
2,453,853 + -1.5
2,453,854 - +0.2
2,453,854 - +1.4
2,453,968 + +0.6
2,454,039 + -1.3

Or see this region's nucleotide sequence