Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20060 and RR42_RS20065 are separated by 102 nucleotides RR42_RS20065 and RR42_RS20070 are separated by 101 nucleotides RR42_RS20070 and RR42_RS20075 are separated by 113 nucleotides RR42_RS20075 and RR42_RS20080 are separated by 31 nucleotides
RR42_RS20060: RR42_RS20060 - glycine cleavage system protein T, at 4,334,296 to 4,335,435
_RS20060
RR42_RS20065: RR42_RS20065 - glycine cleavage system protein H, at 4,335,538 to 4,335,918
_RS20065
RR42_RS20070: RR42_RS20070 - glycine dehydrogenase, at 4,336,020 to 4,338,941
_RS20070
RR42_RS20075: RR42_RS20075 - hypothetical protein, at 4,339,055 to 4,339,597
_RS20075
RR42_RS20080: RR42_RS20080 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 4,339,629 to 4,341,005
_RS20080
Position (kb)
4336
4337
4338
4339 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4335.047 kb on - strand, within RR42_RS20060 at 4335.394 kb on - strand at 4335.562 kb on + strand at 4335.562 kb on + strand at 4335.563 kb on - strand at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.745 kb on + strand, within RR42_RS20065 at 4335.746 kb on - strand, within RR42_RS20065 at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.895 kb on + strand at 4335.896 kb on - strand at 4335.897 kb on + strand at 4335.897 kb on + strand at 4335.897 kb on + strand at 4336.330 kb on - strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.332 kb on + strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.333 kb on - strand, within RR42_RS20070 at 4336.390 kb on - strand, within RR42_RS20070 at 4336.399 kb on - strand, within RR42_RS20070 at 4336.581 kb on + strand, within RR42_RS20070 at 4336.581 kb on + strand, within RR42_RS20070 at 4336.582 kb on - strand, within RR42_RS20070 at 4336.621 kb on + strand, within RR42_RS20070 at 4336.914 kb on + strand, within RR42_RS20070 at 4336.915 kb on - strand, within RR42_RS20070 at 4336.915 kb on - strand, within RR42_RS20070 at 4336.998 kb on + strand, within RR42_RS20070 at 4337.030 kb on + strand, within RR42_RS20070 at 4337.030 kb on + strand, within RR42_RS20070 at 4337.031 kb on - strand, within RR42_RS20070 at 4337.063 kb on + strand, within RR42_RS20070 at 4337.063 kb on + strand, within RR42_RS20070 at 4337.064 kb on - strand, within RR42_RS20070 at 4337.064 kb on - strand, within RR42_RS20070 at 4337.064 kb on - strand, within RR42_RS20070 at 4337.100 kb on + strand, within RR42_RS20070 at 4337.100 kb on + strand, within RR42_RS20070 at 4337.100 kb on + strand, within RR42_RS20070 at 4337.101 kb on - strand, within RR42_RS20070 at 4337.109 kb on + strand, within RR42_RS20070 at 4337.109 kb on + strand, within RR42_RS20070 at 4337.110 kb on - strand, within RR42_RS20070 at 4337.110 kb on - strand, within RR42_RS20070 at 4337.196 kb on + strand, within RR42_RS20070 at 4337.197 kb on - strand, within RR42_RS20070 at 4337.470 kb on - strand, within RR42_RS20070 at 4337.470 kb on - strand, within RR42_RS20070 at 4337.523 kb on + strand, within RR42_RS20070 at 4337.523 kb on + strand, within RR42_RS20070 at 4338.009 kb on + strand, within RR42_RS20070 at 4338.009 kb on + strand, within RR42_RS20070 at 4338.010 kb on - strand, within RR42_RS20070 at 4338.010 kb on - strand, within RR42_RS20070 at 4338.010 kb on - strand, within RR42_RS20070 at 4338.017 kb on + strand, within RR42_RS20070 at 4338.029 kb on - strand, within RR42_RS20070 at 4338.029 kb on - strand, within RR42_RS20070 at 4338.087 kb on + strand, within RR42_RS20070 at 4338.088 kb on - strand, within RR42_RS20070 at 4338.357 kb on + strand, within RR42_RS20070 at 4338.357 kb on + strand, within RR42_RS20070 at 4338.358 kb on - strand, within RR42_RS20070 at 4338.358 kb on - strand, within RR42_RS20070 at 4338.358 kb on - strand, within RR42_RS20070 at 4338.358 kb on - strand, within RR42_RS20070 at 4338.358 kb on - strand, within RR42_RS20070 at 4338.423 kb on + strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.424 kb on - strand, within RR42_RS20070 at 4338.508 kb on + strand, within RR42_RS20070 at 4338.508 kb on + strand, within RR42_RS20070 at 4338.570 kb on + strand, within RR42_RS20070 at 4338.570 kb on + strand, within RR42_RS20070 at 4338.665 kb on + strand at 4338.798 kb on + strand at 4338.798 kb on + strand at 4338.798 kb on + strand at 4338.798 kb on + strand at 4338.798 kb on + strand at 4338.799 kb on - strand at 4338.799 kb on - strand at 4338.850 kb on - strand at 4338.882 kb on + strand at 4338.927 kb on + strand at 4338.927 kb on + strand at 4338.928 kb on - strand at 4338.939 kb on + strand at 4338.939 kb on + strand at 4338.940 kb on - strand at 4339.165 kb on + strand, within RR42_RS20075 at 4339.166 kb on - strand, within RR42_RS20075 at 4339.166 kb on - strand, within RR42_RS20075 at 4339.166 kb on - strand, within RR42_RS20075 at 4339.166 kb on - strand, within RR42_RS20075 at 4339.353 kb on + strand, within RR42_RS20075 at 4339.353 kb on + strand, within RR42_RS20075 at 4339.354 kb on - strand, within RR42_RS20075 at 4339.625 kb on + strand at 4339.779 kb on + strand, within RR42_RS20080 at 4339.779 kb on + strand, within RR42_RS20080 at 4339.779 kb on + strand, within RR42_RS20080 at 4339.780 kb on - strand, within RR42_RS20080 at 4339.780 kb on - strand, within RR42_RS20080 at 4339.780 kb on - strand, within RR42_RS20080
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 4,335,047 - RR42_RS20060 0.66 -3.6 4,335,394 - -1.1 4,335,562 + -0.2 4,335,562 + +0.8 4,335,563 - -0.5 4,335,745 + RR42_RS20065 0.54 +0.9 4,335,745 + RR42_RS20065 0.54 +0.7 4,335,745 + RR42_RS20065 0.54 +0.6 4,335,745 + RR42_RS20065 0.54 +2.1 4,335,745 + RR42_RS20065 0.54 +0.6 4,335,746 - RR42_RS20065 0.55 -2.6 4,335,895 + -0.1 4,335,895 + +1.5 4,335,895 + +1.7 4,335,895 + +0.6 4,335,896 - -1.1 4,335,897 + +0.6 4,335,897 + -0.5 4,335,897 + +1.2 4,336,330 - RR42_RS20070 0.11 -2.3 4,336,332 + RR42_RS20070 0.11 -1.1 4,336,332 + RR42_RS20070 0.11 -1.9 4,336,332 + RR42_RS20070 0.11 -1.0 4,336,332 + RR42_RS20070 0.11 -2.7 4,336,332 + RR42_RS20070 0.11 -1.7 4,336,332 + RR42_RS20070 0.11 -3.8 4,336,333 - RR42_RS20070 0.11 -2.4 4,336,333 - RR42_RS20070 0.11 +1.2 4,336,333 - RR42_RS20070 0.11 -1.7 4,336,390 - RR42_RS20070 0.13 -2.2 4,336,399 - RR42_RS20070 0.13 -3.3 4,336,581 + RR42_RS20070 0.19 -1.5 4,336,581 + RR42_RS20070 0.19 -3.9 4,336,582 - RR42_RS20070 0.19 -0.7 4,336,621 + RR42_RS20070 0.21 -2.5 4,336,914 + RR42_RS20070 0.31 -1.7 4,336,915 - RR42_RS20070 0.31 -1.9 4,336,915 - RR42_RS20070 0.31 +0.2 4,336,998 + RR42_RS20070 0.33 -3.2 4,337,030 + RR42_RS20070 0.35 -1.5 4,337,030 + RR42_RS20070 0.35 -0.9 4,337,031 - RR42_RS20070 0.35 -3.4 4,337,063 + RR42_RS20070 0.36 -0.8 4,337,063 + RR42_RS20070 0.36 -1.5 4,337,064 - RR42_RS20070 0.36 -1.1 4,337,064 - RR42_RS20070 0.36 -0.6 4,337,064 - RR42_RS20070 0.36 -2.5 4,337,100 + RR42_RS20070 0.37 -1.2 4,337,100 + RR42_RS20070 0.37 -0.6 4,337,100 + RR42_RS20070 0.37 -2.7 4,337,101 - RR42_RS20070 0.37 -0.6 4,337,109 + RR42_RS20070 0.37 +0.1 4,337,109 + RR42_RS20070 0.37 -1.6 4,337,110 - RR42_RS20070 0.37 -1.2 4,337,110 - RR42_RS20070 0.37 -2.7 4,337,196 + RR42_RS20070 0.40 -0.2 4,337,197 - RR42_RS20070 0.40 -1.6 4,337,470 - RR42_RS20070 0.50 -2.6 4,337,470 - RR42_RS20070 0.50 -3.2 4,337,523 + RR42_RS20070 0.51 -2.1 4,337,523 + RR42_RS20070 0.51 -2.1 4,338,009 + RR42_RS20070 0.68 -0.1 4,338,009 + RR42_RS20070 0.68 -2.6 4,338,010 - RR42_RS20070 0.68 -0.4 4,338,010 - RR42_RS20070 0.68 -3.3 4,338,010 - RR42_RS20070 0.68 +0.5 4,338,017 + RR42_RS20070 0.68 -1.8 4,338,029 - RR42_RS20070 0.69 -1.4 4,338,029 - RR42_RS20070 0.69 -1.4 4,338,087 + RR42_RS20070 0.71 -0.4 4,338,088 - RR42_RS20070 0.71 -1.0 4,338,357 + RR42_RS20070 0.80 -0.0 4,338,357 + RR42_RS20070 0.80 -0.7 4,338,358 - RR42_RS20070 0.80 -0.8 4,338,358 - RR42_RS20070 0.80 -1.9 4,338,358 - RR42_RS20070 0.80 -2.0 4,338,358 - RR42_RS20070 0.80 -0.8 4,338,358 - RR42_RS20070 0.80 -1.6 4,338,423 + RR42_RS20070 0.82 -0.5 4,338,424 - RR42_RS20070 0.82 -2.9 4,338,424 - RR42_RS20070 0.82 +0.2 4,338,424 - RR42_RS20070 0.82 -2.9 4,338,424 - RR42_RS20070 0.82 -1.9 4,338,424 - RR42_RS20070 0.82 -2.4 4,338,424 - RR42_RS20070 0.82 +0.1 4,338,508 + RR42_RS20070 0.85 +0.9 4,338,508 + RR42_RS20070 0.85 +0.2 4,338,570 + RR42_RS20070 0.87 +2.4 4,338,570 + RR42_RS20070 0.87 +1.7 4,338,665 + +0.8 4,338,798 + +1.2 4,338,798 + +2.3 4,338,798 + +1.9 4,338,798 + +2.9 4,338,798 + +1.9 4,338,799 - +0.1 4,338,799 - -0.9 4,338,850 - -2.8 4,338,882 + +2.8 4,338,927 + +3.1 4,338,927 + +2.8 4,338,928 - -2.1 4,338,939 + +3.2 4,338,939 + +3.0 4,338,940 - -1.1 4,339,165 + RR42_RS20075 0.20 +2.4 4,339,166 - RR42_RS20075 0.20 -2.5 4,339,166 - RR42_RS20075 0.20 -1.8 4,339,166 - RR42_RS20075 0.20 -2.5 4,339,166 - RR42_RS20075 0.20 -2.9 4,339,353 + RR42_RS20075 0.55 +2.8 4,339,353 + RR42_RS20075 0.55 +1.6 4,339,354 - RR42_RS20075 0.55 -1.9 4,339,625 + -1.8 4,339,779 + RR42_RS20080 0.11 -2.3 4,339,779 + RR42_RS20080 0.11 -3.6 4,339,779 + RR42_RS20080 0.11 -3.3 4,339,780 - RR42_RS20080 0.11 -4.1 4,339,780 - RR42_RS20080 0.11 -3.5 4,339,780 - RR42_RS20080 0.11 -3.8
Or see this region's nucleotide sequence