Experiment: NLDM_metabolites (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23440 and EX31_RS23445 are separated by 46 nucleotides EX31_RS23445 and EX31_RS23450 are separated by 92 nucleotides
EX31_RS23440: EX31_RS23440 - 2-oxoglutarate dehydrogenase E1 component, at 454,376 to 457,183
_RS23440
EX31_RS23445: EX31_RS23445 - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 457,230 to 458,459
_RS23445
EX31_RS23450: EX31_RS23450 - ADP-forming succinate--CoA ligase subunit beta, at 458,552 to 459,718
_RS23450
Position (kb)
457
458
459 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 456.313 kb on + strand, within EX31_RS23440 at 456.653 kb on + strand, within EX31_RS23440 at 457.077 kb on + strand at 457.204 kb on + strand at 458.346 kb on + strand at 458.346 kb on + strand at 458.457 kb on + strand at 458.457 kb on + strand at 458.458 kb on - strand at 458.534 kb on - strand at 458.802 kb on - strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.930 kb on - strand, within EX31_RS23450 at 459.305 kb on + strand, within EX31_RS23450 at 459.305 kb on + strand, within EX31_RS23450 at 459.306 kb on - strand, within EX31_RS23450 at 459.306 kb on - strand, within EX31_RS23450
Per-strain Table
Position Strand Gene LocusTag Fraction NLDM_metabolites (C) remove 456,313 + EX31_RS23440 0.69 -0.3 456,653 + EX31_RS23440 0.81 +1.2 457,077 + +0.2 457,204 + -1.3 458,346 + +3.4 458,346 + +2.2 458,457 + -1.6 458,457 + +0.7 458,458 - +1.5 458,534 - +2.0 458,802 - EX31_RS23450 0.21 +0.2 458,929 + EX31_RS23450 0.32 +5.1 458,929 + EX31_RS23450 0.32 +2.1 458,929 + EX31_RS23450 0.32 +0.9 458,930 - EX31_RS23450 0.32 -0.3 459,305 + EX31_RS23450 0.65 -0.7 459,305 + EX31_RS23450 0.65 -0.3 459,306 - EX31_RS23450 0.65 +0.2 459,306 - EX31_RS23450 0.65 -2.1
Or see this region's nucleotide sequence