Strain Fitness in Rahnella sp. WP5 around EX31_RS16680

Experiment: NLDM_metabolites (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS16670 and EX31_RS16675 are separated by 99 nucleotidesEX31_RS16675 and EX31_RS16680 are separated by 55 nucleotidesEX31_RS16680 and EX31_RS16685 overlap by 4 nucleotidesEX31_RS16685 and EX31_RS16690 are separated by 15 nucleotides EX31_RS16670: EX31_RS16670 - NAD(P)-dependent alcohol dehydrogenase, at 16,779 to 17,837 _RS16670 EX31_RS16675: EX31_RS16675 - histidine utilization repressor, at 17,937 to 18,686 _RS16675 EX31_RS16680: EX31_RS16680 - enoyl-CoA hydratase, at 18,742 to 19,512 _RS16680 EX31_RS16685: EX31_RS16685 - 3-hydroxyacyl-CoA dehydrogenase family protein, at 19,509 to 20,402 _RS16685 EX31_RS16690: EX31_RS16690 - CoA transferase, at 20,418 to 21,620 _RS16690 Position (kb) 18 19 20Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 17.756 kb on + strandat 17.756 kb on + strandat 17.813 kb on - strandat 17.869 kb on - strandat 17.873 kb on - strandat 17.874 kb on + strandat 17.875 kb on - strandat 17.945 kb on + strandat 17.980 kb on + strandat 18.110 kb on + strand, within EX31_RS16675at 18.110 kb on + strand, within EX31_RS16675at 18.111 kb on - strand, within EX31_RS16675at 18.111 kb on - strand, within EX31_RS16675at 18.184 kb on - strand, within EX31_RS16675at 18.470 kb on - strand, within EX31_RS16675at 18.504 kb on + strand, within EX31_RS16675at 18.621 kb on - strandat 18.622 kb on + strandat 18.622 kb on + strandat 18.623 kb on - strandat 18.623 kb on - strandat 18.623 kb on - strandat 18.700 kb on + strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.701 kb on - strandat 18.743 kb on + strandat 18.843 kb on - strand, within EX31_RS16680at 19.280 kb on + strand, within EX31_RS16680at 19.281 kb on - strand, within EX31_RS16680at 19.281 kb on - strand, within EX31_RS16680at 19.378 kb on - strand, within EX31_RS16680at 19.778 kb on + strand, within EX31_RS16685at 19.785 kb on + strand, within EX31_RS16685at 19.858 kb on + strand, within EX31_RS16685at 19.859 kb on - strand, within EX31_RS16685at 19.859 kb on - strand, within EX31_RS16685at 19.896 kb on - strand, within EX31_RS16685at 19.956 kb on - strand, within EX31_RS16685at 20.340 kb on + strandat 20.405 kb on + strandat 20.406 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NLDM_metabolites (C)
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17,756 + +0.9
17,756 + -1.1
17,813 - -1.7
17,869 - -1.4
17,873 - +0.6
17,874 + +1.1
17,875 - +1.7
17,945 + -2.8
17,980 + -1.7
18,110 + EX31_RS16675 0.23 +0.8
18,110 + EX31_RS16675 0.23 +1.4
18,111 - EX31_RS16675 0.23 +1.4
18,111 - EX31_RS16675 0.23 +1.5
18,184 - EX31_RS16675 0.33 -2.1
18,470 - EX31_RS16675 0.71 +2.5
18,504 + EX31_RS16675 0.76 +0.7
18,621 - +2.0
18,622 + -0.7
18,622 + +1.7
18,623 - -0.7
18,623 - +0.9
18,623 - -0.1
18,700 + +0.9
18,701 - -1.7
18,701 - +0.9
18,701 - -2.3
18,701 - +2.5
18,743 + +3.7
18,843 - EX31_RS16680 0.13 +3.1
19,280 + EX31_RS16680 0.70 -1.7
19,281 - EX31_RS16680 0.70 -2.5
19,281 - EX31_RS16680 0.70 -1.7
19,378 - EX31_RS16680 0.82 +0.9
19,778 + EX31_RS16685 0.30 -2.1
19,785 + EX31_RS16685 0.31 -1.1
19,858 + EX31_RS16685 0.39 +1.7
19,859 - EX31_RS16685 0.39 +1.1
19,859 - EX31_RS16685 0.39 -0.1
19,896 - EX31_RS16685 0.43 +1.1
19,956 - EX31_RS16685 0.50 -1.1
20,340 + -2.4
20,405 + -2.5
20,406 - +0.9

Or see this region's nucleotide sequence