Experiment: L-Valine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14530 and CA265_RS14535 are separated by 138 nucleotides CA265_RS14535 and CA265_RS14540 are separated by 173 nucleotides CA265_RS14540 and CA265_RS14545 overlap by 1 nucleotides CA265_RS14545 and CA265_RS14550 are separated by 53 nucleotides
CA265_RS14530: CA265_RS14530 - nicotinate-nucleotide diphosphorylase (carboxylating), at 3,660,836 to 3,661,678
_RS14530
CA265_RS14535: CA265_RS14535 - DUF4783 domain-containing protein, at 3,661,817 to 3,662,215
_RS14535
CA265_RS14540: CA265_RS14540 - phosphoglycerate mutase (2,3-diphosphoglycerate-independent), at 3,662,389 to 3,663,912
_RS14540
CA265_RS14545: CA265_RS14545 - hypothetical protein, at 3,663,912 to 3,664,442
_RS14545
CA265_RS14550: CA265_RS14550 - hypothetical protein, at 3,664,496 to 3,665,116
_RS14550
Position (kb)
3662
3663
3664 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3661.414 kb on + strand, within CA265_RS14530 at 3661.475 kb on + strand, within CA265_RS14530 at 3661.691 kb on - strand at 3661.718 kb on - strand at 3661.730 kb on - strand at 3661.753 kb on - strand at 3661.757 kb on + strand at 3661.757 kb on + strand at 3661.759 kb on + strand at 3661.913 kb on - strand, within CA265_RS14535 at 3662.170 kb on - strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.186 kb on - strand at 3662.202 kb on - strand at 3662.239 kb on - strand at 3662.245 kb on - strand at 3662.307 kb on + strand at 3662.318 kb on + strand at 3662.318 kb on + strand at 3662.344 kb on + strand at 3664.002 kb on + strand, within CA265_RS14545 at 3664.003 kb on - strand, within CA265_RS14545 at 3664.012 kb on + strand, within CA265_RS14545 at 3664.196 kb on - strand, within CA265_RS14545 at 3664.217 kb on + strand, within CA265_RS14545 at 3664.218 kb on - strand, within CA265_RS14545 at 3664.218 kb on - strand, within CA265_RS14545 at 3664.223 kb on + strand, within CA265_RS14545 at 3664.224 kb on - strand, within CA265_RS14545 at 3664.301 kb on + strand, within CA265_RS14545 at 3664.301 kb on + strand, within CA265_RS14545 at 3664.326 kb on + strand, within CA265_RS14545 at 3664.345 kb on + strand, within CA265_RS14545 at 3664.351 kb on - strand, within CA265_RS14545 at 3664.369 kb on - strand, within CA265_RS14545 at 3664.369 kb on - strand, within CA265_RS14545 at 3664.503 kb on - strand at 3664.547 kb on - strand at 3664.548 kb on + strand at 3664.557 kb on - strand at 3664.571 kb on + strand, within CA265_RS14550 at 3664.595 kb on - strand at 3664.677 kb on + strand, within CA265_RS14550 at 3664.682 kb on + strand, within CA265_RS14550 at 3664.724 kb on - strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.783 kb on + strand, within CA265_RS14550 at 3664.784 kb on - strand, within CA265_RS14550 at 3664.839 kb on + strand, within CA265_RS14550 at 3664.894 kb on + strand, within CA265_RS14550 at 3664.894 kb on + strand, within CA265_RS14550
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (N) remove 3,661,414 + CA265_RS14530 0.69 +1.5 3,661,475 + CA265_RS14530 0.76 -0.8 3,661,691 - -1.2 3,661,718 - -2.6 3,661,730 - -0.9 3,661,753 - -1.5 3,661,757 + -0.2 3,661,757 + +1.0 3,661,759 + -0.0 3,661,913 - CA265_RS14535 0.24 +0.5 3,662,170 - CA265_RS14535 0.88 +0.4 3,662,171 + CA265_RS14535 0.89 +1.3 3,662,171 + CA265_RS14535 0.89 -0.2 3,662,186 - +1.9 3,662,202 - +1.3 3,662,239 - +1.2 3,662,245 - -0.1 3,662,307 + -0.5 3,662,318 + -0.0 3,662,318 + -1.2 3,662,344 + +0.4 3,664,002 + CA265_RS14545 0.17 +0.0 3,664,003 - CA265_RS14545 0.17 +1.5 3,664,012 + CA265_RS14545 0.19 +0.2 3,664,196 - CA265_RS14545 0.53 -0.7 3,664,217 + CA265_RS14545 0.57 +1.7 3,664,218 - CA265_RS14545 0.58 -0.2 3,664,218 - CA265_RS14545 0.58 +0.4 3,664,223 + CA265_RS14545 0.59 +0.7 3,664,224 - CA265_RS14545 0.59 +0.5 3,664,301 + CA265_RS14545 0.73 +0.1 3,664,301 + CA265_RS14545 0.73 +1.7 3,664,326 + CA265_RS14545 0.78 +0.8 3,664,345 + CA265_RS14545 0.82 +2.7 3,664,351 - CA265_RS14545 0.83 +1.1 3,664,369 - CA265_RS14545 0.86 +1.1 3,664,369 - CA265_RS14545 0.86 +0.8 3,664,503 - +0.3 3,664,547 - -1.9 3,664,548 + +1.3 3,664,557 - -0.2 3,664,571 + CA265_RS14550 0.12 -1.1 3,664,595 - +2.3 3,664,677 + CA265_RS14550 0.29 -0.3 3,664,682 + CA265_RS14550 0.30 -1.2 3,664,724 - CA265_RS14550 0.37 -0.9 3,664,783 + CA265_RS14550 0.46 -2.5 3,664,783 + CA265_RS14550 0.46 -1.0 3,664,783 + CA265_RS14550 0.46 -0.3 3,664,783 + CA265_RS14550 0.46 -1.5 3,664,784 - CA265_RS14550 0.46 +0.7 3,664,839 + CA265_RS14550 0.55 +0.3 3,664,894 + CA265_RS14550 0.64 +0.1 3,664,894 + CA265_RS14550 0.64 -1.9
Or see this region's nucleotide sequence