Experiment: CDM1 without Adenine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13415 and BBR_RS20775 are separated by 157 nucleotides BBR_RS20775 and BBR_RS13420 are separated by 9 nucleotides BBR_RS13420 and BBR_RS13425 are separated by 126 nucleotides BBR_RS13425 and BBR_RS13430 are separated by 83 nucleotides BBR_RS13430 and BBR_RS13435 overlap by 4 nucleotides
BBR_RS13415: BBR_RS13415 - hypothetical protein, at 781,069 to 781,701
_RS13415
BBR_RS20775: BBR_RS20775 - DUF4230 domain-containing protein, at 781,859 to 781,969
_RS20775
BBR_RS13420: BBR_RS13420 - DUF4230 domain-containing protein, at 781,979 to 782,266
_RS13420
BBR_RS13425: BBR_RS13425 - hypothetical protein, at 782,393 to 783,205
_RS13425
BBR_RS13430: BBR_RS13430 - hypothetical protein, at 783,289 to 783,948
_RS13430
BBR_RS13435: BBR_RS13435 - hypothetical protein, at 783,945 to 785,315
_RS13435
Position (kb) 782
783
784 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 781.408 kb on + strand, within BBR_RS13415 at 781.408 kb on + strand, within BBR_RS13415 at 781.408 kb on + strand, within BBR_RS13415 at 781.413 kb on - strand, within BBR_RS13415 at 781.416 kb on - strand, within BBR_RS13415 at 781.416 kb on - strand, within BBR_RS13415 at 781.572 kb on + strand, within BBR_RS13415 at 781.597 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.599 kb on - strand, within BBR_RS13415 at 781.628 kb on - strand, within BBR_RS13415 at 781.681 kb on + strand at 781.681 kb on - strand at 781.683 kb on - strand at 781.701 kb on - strand at 781.713 kb on - strand at 781.728 kb on - strand at 781.737 kb on - strand at 781.763 kb on - strand at 781.765 kb on - strand at 781.778 kb on + strand at 781.791 kb on + strand at 781.791 kb on + strand at 781.817 kb on + strand at 781.830 kb on - strand at 781.861 kb on + strand at 781.900 kb on - strand, within BBR_RS20775 at 781.941 kb on + strand, within BBR_RS20775 at 781.941 kb on + strand, within BBR_RS20775 at 782.070 kb on + strand, within BBR_RS13420 at 782.139 kb on - strand, within BBR_RS13420 at 782.155 kb on + strand, within BBR_RS13420 at 782.179 kb on + strand, within BBR_RS13420 at 782.210 kb on - strand, within BBR_RS13420 at 782.242 kb on + strand at 782.247 kb on - strand at 782.260 kb on - strand at 782.339 kb on - strand at 782.408 kb on - strand at 782.417 kb on + strand at 782.424 kb on - strand at 782.445 kb on + strand at 782.499 kb on + strand, within BBR_RS13425 at 782.499 kb on + strand, within BBR_RS13425 at 782.556 kb on + strand, within BBR_RS13425 at 782.676 kb on - strand, within BBR_RS13425 at 782.676 kb on - strand, within BBR_RS13425 at 782.765 kb on + strand, within BBR_RS13425 at 782.788 kb on + strand, within BBR_RS13425 at 782.789 kb on - strand, within BBR_RS13425 at 782.802 kb on - strand, within BBR_RS13425 at 782.919 kb on + strand, within BBR_RS13425 at 782.925 kb on - strand, within BBR_RS13425 at 782.978 kb on + strand, within BBR_RS13425 at 782.989 kb on - strand, within BBR_RS13425 at 783.079 kb on - strand, within BBR_RS13425 at 783.089 kb on + strand, within BBR_RS13425 at 783.094 kb on + strand, within BBR_RS13425 at 783.106 kb on + strand, within BBR_RS13425 at 783.166 kb on + strand at 783.174 kb on - strand at 783.180 kb on - strand at 783.180 kb on - strand at 783.187 kb on + strand at 783.199 kb on + strand at 783.199 kb on + strand at 783.258 kb on - strand at 783.385 kb on - strand, within BBR_RS13430 at 783.391 kb on - strand, within BBR_RS13430 at 783.428 kb on - strand, within BBR_RS13430 at 783.434 kb on + strand, within BBR_RS13430 at 783.444 kb on - strand, within BBR_RS13430 at 783.488 kb on + strand, within BBR_RS13430 at 783.488 kb on + strand, within BBR_RS13430 at 783.497 kb on - strand, within BBR_RS13430 at 783.513 kb on + strand, within BBR_RS13430 at 783.530 kb on + strand, within BBR_RS13430 at 783.533 kb on - strand, within BBR_RS13430 at 783.544 kb on + strand, within BBR_RS13430 at 783.544 kb on + strand, within BBR_RS13430 at 783.580 kb on - strand, within BBR_RS13430 at 783.593 kb on + strand, within BBR_RS13430 at 783.595 kb on + strand, within BBR_RS13430 at 783.598 kb on + strand, within BBR_RS13430 at 783.601 kb on - strand, within BBR_RS13430 at 783.601 kb on - strand, within BBR_RS13430 at 783.605 kb on + strand, within BBR_RS13430 at 783.700 kb on - strand, within BBR_RS13430 at 783.700 kb on - strand, within BBR_RS13430 at 783.751 kb on + strand, within BBR_RS13430 at 783.840 kb on - strand, within BBR_RS13430 at 783.857 kb on + strand, within BBR_RS13430 at 783.900 kb on + strand at 783.900 kb on + strand at 783.942 kb on + strand at 783.942 kb on + strand at 783.960 kb on + strand at 784.007 kb on + strand at 784.009 kb on - strand at 784.090 kb on + strand, within BBR_RS13435 at 784.174 kb on - strand, within BBR_RS13435 at 784.187 kb on + strand, within BBR_RS13435 at 784.195 kb on - strand, within BBR_RS13435
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Adenine remove 781,408 + BBR_RS13415 0.54 -3.2 781,408 + BBR_RS13415 0.54 -0.6 781,408 + BBR_RS13415 0.54 -2.5 781,413 - BBR_RS13415 0.54 +1.4 781,416 - BBR_RS13415 0.55 -1.7 781,416 - BBR_RS13415 0.55 -0.6 781,572 + BBR_RS13415 0.79 -1.0 781,597 - BBR_RS13415 0.83 +0.2 781,599 - BBR_RS13415 0.84 -2.3 781,599 - BBR_RS13415 0.84 +2.1 781,599 - BBR_RS13415 0.84 +0.6 781,628 - BBR_RS13415 0.88 +1.4 781,681 + -0.7 781,681 - +1.5 781,683 - +0.5 781,701 - +0.5 781,713 - -0.0 781,728 - +0.3 781,737 - +0.4 781,763 - -2.6 781,765 - +1.1 781,778 + -2.6 781,791 + -2.4 781,791 + -3.0 781,817 + -3.0 781,830 - -2.6 781,861 + -2.6 781,900 - BBR_RS20775 0.37 -0.2 781,941 + BBR_RS20775 0.74 -2.7 781,941 + BBR_RS20775 0.74 -1.9 782,070 + BBR_RS13420 0.32 -4.4 782,139 - BBR_RS13420 0.56 +0.4 782,155 + BBR_RS13420 0.61 -1.8 782,179 + BBR_RS13420 0.69 -0.8 782,210 - BBR_RS13420 0.80 -3.1 782,242 + -1.4 782,247 - -3.5 782,260 - -3.2 782,339 - -0.7 782,408 - +1.3 782,417 + -2.0 782,424 - +0.2 782,445 + -0.2 782,499 + BBR_RS13425 0.13 -3.2 782,499 + BBR_RS13425 0.13 -0.9 782,556 + BBR_RS13425 0.20 -3.0 782,676 - BBR_RS13425 0.35 -2.1 782,676 - BBR_RS13425 0.35 +0.3 782,765 + BBR_RS13425 0.46 -2.7 782,788 + BBR_RS13425 0.49 +0.6 782,789 - BBR_RS13425 0.49 -2.2 782,802 - BBR_RS13425 0.50 -3.4 782,919 + BBR_RS13425 0.65 -3.3 782,925 - BBR_RS13425 0.65 +0.2 782,978 + BBR_RS13425 0.72 +0.7 782,989 - BBR_RS13425 0.73 -0.4 783,079 - BBR_RS13425 0.84 -2.1 783,089 + BBR_RS13425 0.86 -2.7 783,094 + BBR_RS13425 0.86 -2.4 783,106 + BBR_RS13425 0.88 +0.7 783,166 + +1.5 783,174 - -1.8 783,180 - -2.6 783,180 - -3.1 783,187 + -1.5 783,199 + -1.8 783,199 + +0.2 783,258 - +0.7 783,385 - BBR_RS13430 0.15 -1.2 783,391 - BBR_RS13430 0.15 -1.1 783,428 - BBR_RS13430 0.21 +0.9 783,434 + BBR_RS13430 0.22 -2.1 783,444 - BBR_RS13430 0.23 +0.1 783,488 + BBR_RS13430 0.30 -0.6 783,488 + BBR_RS13430 0.30 -1.3 783,497 - BBR_RS13430 0.32 -0.2 783,513 + BBR_RS13430 0.34 -0.7 783,530 + BBR_RS13430 0.37 -0.6 783,533 - BBR_RS13430 0.37 -1.5 783,544 + BBR_RS13430 0.39 -0.1 783,544 + BBR_RS13430 0.39 -0.1 783,580 - BBR_RS13430 0.44 -1.1 783,593 + BBR_RS13430 0.46 -1.6 783,595 + BBR_RS13430 0.46 +0.1 783,598 + BBR_RS13430 0.47 +0.3 783,601 - BBR_RS13430 0.47 +0.6 783,601 - BBR_RS13430 0.47 -1.1 783,605 + BBR_RS13430 0.48 -1.0 783,700 - BBR_RS13430 0.62 -2.1 783,700 - BBR_RS13430 0.62 -0.3 783,751 + BBR_RS13430 0.70 -2.0 783,840 - BBR_RS13430 0.83 -2.2 783,857 + BBR_RS13430 0.86 +1.1 783,900 + +0.1 783,900 + +0.4 783,942 + -2.3 783,942 + -2.2 783,960 + -0.1 784,007 + -1.4 784,009 - -0.6 784,090 + BBR_RS13435 0.11 -0.3 784,174 - BBR_RS13435 0.17 +0.5 784,187 + BBR_RS13435 0.18 -0.1 784,195 - BBR_RS13435 0.18 +2.5
Or see this region's nucleotide sequence