Experiment: CDM1 without Adenine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12355 and BBR_RS12360 are separated by 170 nucleotides BBR_RS12360 and BBR_RS12365 are separated by 89 nucleotides BBR_RS12365 and BBR_RS12370 are separated by 115 nucleotides BBR_RS12370 and BBR_RS12375 are separated by 5 nucleotides
BBR_RS12355: BBR_RS12355 - ribonuclease III, at 524,160 to 524,903
_RS12355
BBR_RS12360: BBR_RS12360 - 50S ribosomal protein L32, at 525,074 to 525,268
_RS12360
BBR_RS12365: BBR_RS12365 - DUF177 domain-containing protein, at 525,358 to 525,978
_RS12365
BBR_RS12370: BBR_RS12370 - cell division protein, at 526,094 to 526,879
_RS12370
BBR_RS12375: BBR_RS12375 - pantetheine-phosphate adenylyltransferase, at 526,885 to 527,385
_RS12375
Position (kb)
525
526 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 524.465 kb on + strand, within BBR_RS12355 at 524.468 kb on - strand, within BBR_RS12355 at 524.552 kb on - strand, within BBR_RS12355 at 524.554 kb on + strand, within BBR_RS12355 at 524.621 kb on - strand, within BBR_RS12355 at 524.654 kb on - strand, within BBR_RS12355 at 524.701 kb on - strand, within BBR_RS12355 at 524.789 kb on + strand, within BBR_RS12355 at 524.810 kb on + strand, within BBR_RS12355 at 524.836 kb on + strand at 524.838 kb on + strand at 524.932 kb on - strand at 524.936 kb on + strand at 524.943 kb on + strand at 524.944 kb on - strand at 524.944 kb on - strand at 524.948 kb on + strand at 524.948 kb on + strand at 524.963 kb on - strand at 524.973 kb on - strand at 524.975 kb on - strand at 524.977 kb on + strand at 524.982 kb on - strand at 524.983 kb on + strand at 525.379 kb on + strand at 525.382 kb on - strand at 525.388 kb on - strand at 525.394 kb on - strand at 525.456 kb on + strand, within BBR_RS12365 at 525.456 kb on + strand, within BBR_RS12365 at 525.458 kb on - strand, within BBR_RS12365 at 525.461 kb on + strand, within BBR_RS12365 at 525.464 kb on - strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.475 kb on + strand, within BBR_RS12365 at 525.476 kb on - strand, within BBR_RS12365 at 525.478 kb on - strand, within BBR_RS12365 at 525.480 kb on + strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.488 kb on - strand, within BBR_RS12365 at 525.502 kb on - strand, within BBR_RS12365 at 525.528 kb on - strand, within BBR_RS12365 at 525.529 kb on - strand, within BBR_RS12365 at 525.533 kb on - strand, within BBR_RS12365 at 525.602 kb on - strand, within BBR_RS12365 at 525.612 kb on - strand, within BBR_RS12365 at 525.662 kb on + strand, within BBR_RS12365 at 525.667 kb on + strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.675 kb on - strand, within BBR_RS12365 at 525.676 kb on - strand, within BBR_RS12365 at 525.716 kb on + strand, within BBR_RS12365 at 525.721 kb on + strand, within BBR_RS12365 at 525.735 kb on + strand, within BBR_RS12365 at 525.743 kb on - strand, within BBR_RS12365 at 525.844 kb on - strand, within BBR_RS12365 at 525.850 kb on - strand, within BBR_RS12365 at 525.854 kb on - strand, within BBR_RS12365 at 525.862 kb on - strand, within BBR_RS12365 at 525.890 kb on - strand, within BBR_RS12365 at 525.896 kb on + strand, within BBR_RS12365 at 525.905 kb on + strand, within BBR_RS12365 at 525.939 kb on - strand at 526.027 kb on - strand at 526.034 kb on + strand at 526.065 kb on + strand at 526.070 kb on + strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.078 kb on - strand at 526.078 kb on - strand at 526.082 kb on + strand at 526.082 kb on + strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.110 kb on - strand at 526.131 kb on + strand at 526.733 kb on - strand, within BBR_RS12370 at 526.891 kb on - strand at 526.896 kb on - strand at 526.898 kb on + strand at 526.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Adenine remove 524,465 + BBR_RS12355 0.41 -1.4 524,468 - BBR_RS12355 0.41 -0.3 524,552 - BBR_RS12355 0.53 -1.4 524,554 + BBR_RS12355 0.53 +2.7 524,621 - BBR_RS12355 0.62 +0.9 524,654 - BBR_RS12355 0.66 -0.7 524,701 - BBR_RS12355 0.73 +0.7 524,789 + BBR_RS12355 0.85 -1.6 524,810 + BBR_RS12355 0.87 -2.0 524,836 + +1.0 524,838 + -0.8 524,932 - +0.4 524,936 + +0.1 524,943 + -2.7 524,944 - -0.7 524,944 - -1.4 524,948 + -0.8 524,948 + -0.2 524,963 - +0.7 524,973 - -2.0 524,975 - -0.4 524,977 + +0.1 524,982 - +0.8 524,983 + -2.4 525,379 + -3.2 525,382 - -0.5 525,388 - +1.2 525,394 - +1.2 525,456 + BBR_RS12365 0.16 -2.9 525,456 + BBR_RS12365 0.16 -3.1 525,458 - BBR_RS12365 0.16 -2.1 525,461 + BBR_RS12365 0.17 -2.0 525,464 - BBR_RS12365 0.17 +0.1 525,475 + BBR_RS12365 0.19 -1.5 525,475 + BBR_RS12365 0.19 -2.8 525,476 - BBR_RS12365 0.19 -1.0 525,478 - BBR_RS12365 0.19 -2.8 525,480 + BBR_RS12365 0.20 -2.0 525,488 - BBR_RS12365 0.21 +0.7 525,488 - BBR_RS12365 0.21 -1.0 525,502 - BBR_RS12365 0.23 +0.2 525,528 - BBR_RS12365 0.27 -2.2 525,529 - BBR_RS12365 0.28 -2.2 525,533 - BBR_RS12365 0.28 -2.8 525,602 - BBR_RS12365 0.39 -3.0 525,612 - BBR_RS12365 0.41 -2.2 525,662 + BBR_RS12365 0.49 -3.3 525,667 + BBR_RS12365 0.50 -3.1 525,675 - BBR_RS12365 0.51 -1.7 525,675 - BBR_RS12365 0.51 -0.3 525,676 - BBR_RS12365 0.51 -2.5 525,716 + BBR_RS12365 0.58 -1.3 525,721 + BBR_RS12365 0.58 -2.0 525,735 + BBR_RS12365 0.61 -2.0 525,743 - BBR_RS12365 0.62 -2.6 525,844 - BBR_RS12365 0.78 -2.7 525,850 - BBR_RS12365 0.79 -2.4 525,854 - BBR_RS12365 0.80 -3.1 525,862 - BBR_RS12365 0.81 -3.9 525,890 - BBR_RS12365 0.86 -0.4 525,896 + BBR_RS12365 0.87 -2.1 525,905 + BBR_RS12365 0.88 -2.9 525,939 - -0.7 526,027 - -2.0 526,034 + +0.4 526,065 + +0.7 526,070 + +0.2 526,073 - -0.0 526,073 - +1.6 526,078 - +0.2 526,078 - +0.2 526,082 + +0.4 526,082 + -0.3 526,090 - +1.5 526,090 - +0.2 526,090 - +2.0 526,110 - +1.8 526,131 + -3.1 526,733 - BBR_RS12370 0.81 -1.5 526,891 - -4.9 526,896 - -0.1 526,898 + +0.8 526,916 + -0.9
Or see this region's nucleotide sequence