Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_18010 and AO356_18015 are separated by 152 nucleotides AO356_18015 and AO356_18020 are separated by 114 nucleotides AO356_18020 and AO356_18025 are separated by 0 nucleotides
AO356_18010: AO356_18010 - hypothetical protein, at 4,063,609 to 4,064,355
_18010
AO356_18015: AO356_18015 - crossover junction endodeoxyribonuclease RuvC, at 4,064,508 to 4,065,032
_18015
AO356_18020: AO356_18020 - ATP-dependent DNA helicase RuvA, at 4,065,147 to 4,065,755
_18020
AO356_18025: AO356_18025 - ATP-dependent DNA helicase RuvB, at 4,065,756 to 4,066,817
_18025
Position (kb)
4065
4066 Strain fitness (log2 ratio)
-2
-1
0
1 at 4064.149 kb on + strand, within AO356_18010 at 4064.149 kb on + strand, within AO356_18010 at 4064.149 kb on + strand, within AO356_18010 at 4064.149 kb on + strand, within AO356_18010 at 4064.149 kb on + strand, within AO356_18010 at 4064.150 kb on - strand, within AO356_18010 at 4064.150 kb on - strand, within AO356_18010 at 4064.150 kb on - strand, within AO356_18010 at 4064.150 kb on - strand, within AO356_18010 at 4064.150 kb on - strand, within AO356_18010 at 4064.308 kb on + strand at 4064.309 kb on - strand at 4064.367 kb on + strand at 4064.367 kb on + strand at 4064.368 kb on - strand at 4064.368 kb on - strand at 4064.414 kb on + strand at 4064.414 kb on + strand at 4064.414 kb on + strand at 4064.415 kb on - strand at 4064.415 kb on - strand at 4064.415 kb on - strand at 4064.516 kb on - strand at 4064.516 kb on - strand at 4064.590 kb on - strand, within AO356_18015 at 4064.590 kb on - strand, within AO356_18015 at 4064.683 kb on - strand, within AO356_18015 at 4065.033 kb on + strand at 4065.034 kb on - strand at 4065.103 kb on + strand at 4065.208 kb on + strand, within AO356_18020 at 4065.222 kb on + strand, within AO356_18020 at 4065.222 kb on + strand, within AO356_18020 at 4065.322 kb on + strand, within AO356_18020 at 4065.322 kb on + strand, within AO356_18020 at 4065.322 kb on + strand, within AO356_18020 at 4065.322 kb on + strand, within AO356_18020 at 4065.323 kb on - strand, within AO356_18020 at 4065.657 kb on + strand, within AO356_18020 at 4065.658 kb on - strand, within AO356_18020 at 4065.753 kb on + strand at 4065.753 kb on + strand at 4065.754 kb on - strand at 4065.852 kb on + strand at 4066.087 kb on + strand, within AO356_18025 at 4066.311 kb on + strand, within AO356_18025 at 4066.390 kb on - strand, within AO356_18025 at 4066.390 kb on - strand, within AO356_18025 at 4066.690 kb on - strand, within AO356_18025 at 4066.734 kb on + strand at 4066.734 kb on + strand at 4066.734 kb on + strand at 4066.735 kb on - strand at 4066.735 kb on - strand at 4066.750 kb on + strand at 4066.751 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,064,149 + AO356_18010 0.72 -0.6 4,064,149 + AO356_18010 0.72 -0.3 4,064,149 + AO356_18010 0.72 -1.7 4,064,149 + AO356_18010 0.72 +0.2 4,064,149 + AO356_18010 0.72 +1.0 4,064,150 - AO356_18010 0.72 -0.3 4,064,150 - AO356_18010 0.72 -0.5 4,064,150 - AO356_18010 0.72 -0.6 4,064,150 - AO356_18010 0.72 -0.9 4,064,150 - AO356_18010 0.72 +0.4 4,064,308 + -1.9 4,064,309 - +0.5 4,064,367 + -0.7 4,064,367 + +0.4 4,064,368 - +0.8 4,064,368 - +1.1 4,064,414 + -0.3 4,064,414 + -0.1 4,064,414 + +0.1 4,064,415 - +0.6 4,064,415 - +0.3 4,064,415 - -0.7 4,064,516 - +0.1 4,064,516 - -0.0 4,064,590 - AO356_18015 0.16 -1.6 4,064,590 - AO356_18015 0.16 -1.3 4,064,683 - AO356_18015 0.33 -2.1 4,065,033 + +0.0 4,065,034 - -1.6 4,065,103 + +0.6 4,065,208 + AO356_18020 0.10 -1.8 4,065,222 + AO356_18020 0.12 -1.5 4,065,222 + AO356_18020 0.12 -0.5 4,065,322 + AO356_18020 0.29 -0.9 4,065,322 + AO356_18020 0.29 -0.5 4,065,322 + AO356_18020 0.29 +0.3 4,065,322 + AO356_18020 0.29 -1.6 4,065,323 - AO356_18020 0.29 +0.3 4,065,657 + AO356_18020 0.84 -0.1 4,065,658 - AO356_18020 0.84 -1.1 4,065,753 + -1.6 4,065,753 + -1.7 4,065,754 - -1.3 4,065,852 + -0.1 4,066,087 + AO356_18025 0.31 +0.5 4,066,311 + AO356_18025 0.52 -1.8 4,066,390 - AO356_18025 0.60 -2.1 4,066,390 - AO356_18025 0.60 -0.8 4,066,690 - AO356_18025 0.88 -1.9 4,066,734 + -0.9 4,066,734 + +0.3 4,066,734 + -1.3 4,066,735 - +0.4 4,066,735 - -1.7 4,066,750 + +0.2 4,066,751 - -0.1
Or see this region's nucleotide sequence