Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12050 and RR42_RS12055 overlap by 4 nucleotides RR42_RS12055 and RR42_RS12060 are separated by 92 nucleotides RR42_RS12060 and RR42_RS12065 are separated by 195 nucleotides RR42_RS12065 and RR42_RS12070 are separated by 47 nucleotides
RR42_RS12050: RR42_RS12050 - DNA polymerase, at 2,583,750 to 2,584,703
_RS12050
RR42_RS12055: RR42_RS12055 - acyltransferase, at 2,584,700 to 2,585,194
_RS12055
RR42_RS12060: RR42_RS12060 - O-sialoglycoprotein endopeptidase, at 2,585,287 to 2,586,054
_RS12060
RR42_RS12065: RR42_RS12065 - acyl-CoA-binding protein, at 2,586,250 to 2,586,519
_RS12065
RR42_RS12070: RR42_RS12070 - MFS transporter, at 2,586,567 to 2,588,036
_RS12070
Position (kb)
2585
2586
2587 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2584.344 kb on - strand, within RR42_RS12050 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.777 kb on - strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.779 kb on + strand, within RR42_RS12055 at 2584.780 kb on - strand, within RR42_RS12055 at 2584.822 kb on - strand, within RR42_RS12055 at 2584.960 kb on + strand, within RR42_RS12055 at 2585.101 kb on - strand, within RR42_RS12055 at 2586.176 kb on + strand at 2586.176 kb on + strand at 2586.177 kb on - strand at 2586.177 kb on - strand at 2586.340 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.415 kb on + strand, within RR42_RS12065 at 2586.475 kb on + strand, within RR42_RS12065 at 2586.476 kb on - strand, within RR42_RS12065 at 2586.817 kb on + strand, within RR42_RS12070 at 2586.910 kb on + strand, within RR42_RS12070
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 2,584,344 - RR42_RS12050 0.62 +0.5 2,584,777 - RR42_RS12055 0.16 -1.3 2,584,777 - RR42_RS12055 0.16 -0.6 2,584,779 + RR42_RS12055 0.16 -1.1 2,584,779 + RR42_RS12055 0.16 -1.4 2,584,780 - RR42_RS12055 0.16 -0.2 2,584,822 - RR42_RS12055 0.25 -0.1 2,584,960 + RR42_RS12055 0.53 -3.5 2,585,101 - RR42_RS12055 0.81 -2.6 2,586,176 + -1.2 2,586,176 + -0.5 2,586,177 - +0.8 2,586,177 - -0.8 2,586,340 + RR42_RS12065 0.33 +2.7 2,586,415 + RR42_RS12065 0.61 +0.2 2,586,415 + RR42_RS12065 0.61 +0.4 2,586,475 + RR42_RS12065 0.83 +0.1 2,586,476 - RR42_RS12065 0.84 -0.9 2,586,817 + RR42_RS12070 0.17 +0.1 2,586,910 + RR42_RS12070 0.23 -0.3
Or see this region's nucleotide sequence