Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07360 and MMJJ_RS07365 are separated by 17 nucleotides MMJJ_RS07365 and MMJJ_RS07370 are separated by 15 nucleotides MMJJ_RS07370 and MMJJ_RS07375 are separated by 138 nucleotides
MMJJ_RS07360: MMJJ_RS07360 - TIGR00269 family protein, at 1,351,552 to 1,352,487
_RS07360
MMJJ_RS07365: MMJJ_RS07365 - CatB-related O-acetyltransferase, at 1,352,505 to 1,353,185
_RS07365
MMJJ_RS07370: MMJJ_RS07370 - tRNA 4-thiouridine(8) synthase ThiI, at 1,353,201 to 1,354,352
_RS07370
MMJJ_RS07375: MMJJ_RS07375 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,354,491 to 1,355,465
_RS07375
Position (kb)
1353
1354
1355 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1352.331 kb on + strand, within MMJJ_RS07360 at 1352.425 kb on + strand at 1352.485 kb on - strand at 1352.485 kb on - strand at 1352.493 kb on - strand at 1352.526 kb on - strand at 1352.526 kb on - strand at 1352.527 kb on + strand at 1352.556 kb on + strand at 1352.646 kb on + strand, within MMJJ_RS07365 at 1352.673 kb on - strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.674 kb on + strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.678 kb on - strand, within MMJJ_RS07365 at 1352.679 kb on + strand, within MMJJ_RS07365 at 1352.679 kb on + strand, within MMJJ_RS07365 at 1352.680 kb on - strand, within MMJJ_RS07365 at 1352.705 kb on - strand, within MMJJ_RS07365 at 1352.705 kb on - strand, within MMJJ_RS07365 at 1352.706 kb on + strand, within MMJJ_RS07365 at 1352.754 kb on + strand, within MMJJ_RS07365 at 1352.760 kb on - strand, within MMJJ_RS07365 at 1352.761 kb on + strand, within MMJJ_RS07365 at 1352.779 kb on + strand, within MMJJ_RS07365 at 1352.839 kb on + strand, within MMJJ_RS07365 at 1352.863 kb on + strand, within MMJJ_RS07365 at 1352.875 kb on - strand, within MMJJ_RS07365 at 1352.875 kb on - strand, within MMJJ_RS07365 at 1352.876 kb on + strand, within MMJJ_RS07365 at 1352.876 kb on + strand, within MMJJ_RS07365 at 1352.894 kb on + strand, within MMJJ_RS07365 at 1352.919 kb on - strand, within MMJJ_RS07365 at 1352.919 kb on - strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.920 kb on + strand, within MMJJ_RS07365 at 1352.961 kb on + strand, within MMJJ_RS07365 at 1352.964 kb on - strand, within MMJJ_RS07365 at 1353.004 kb on - strand, within MMJJ_RS07365 at 1353.004 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.011 kb on - strand, within MMJJ_RS07365 at 1353.012 kb on + strand, within MMJJ_RS07365 at 1353.053 kb on + strand, within MMJJ_RS07365 at 1353.056 kb on - strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.057 kb on + strand, within MMJJ_RS07365 at 1353.124 kb on - strand at 1353.149 kb on - strand at 1353.152 kb on - strand at 1353.161 kb on + strand at 1353.176 kb on - strand at 1353.188 kb on - strand at 1353.188 kb on - strand at 1353.188 kb on - strand at 1353.190 kb on - strand at 1353.197 kb on - strand at 1353.197 kb on - strand at 1353.285 kb on - strand at 1353.541 kb on - strand, within MMJJ_RS07370 at 1353.565 kb on - strand, within MMJJ_RS07370 at 1353.627 kb on - strand, within MMJJ_RS07370 at 1353.634 kb on + strand at 1353.663 kb on - strand, within MMJJ_RS07370 at 1353.726 kb on - strand at 1353.758 kb on + strand, within MMJJ_RS07370 at 1353.758 kb on + strand, within MMJJ_RS07370 at 1353.913 kb on + strand, within MMJJ_RS07370 at 1353.918 kb on - strand at 1354.110 kb on - strand, within MMJJ_RS07370 at 1354.111 kb on + strand, within MMJJ_RS07370 at 1354.111 kb on + strand, within MMJJ_RS07370 at 1354.115 kb on + strand, within MMJJ_RS07370 at 1354.117 kb on - strand at 1354.128 kb on + strand, within MMJJ_RS07370 at 1354.134 kb on - strand, within MMJJ_RS07370 at 1354.198 kb on + strand, within MMJJ_RS07370 at 1354.354 kb on + strand at 1354.386 kb on - strand at 1354.387 kb on + strand at 1354.396 kb on - strand at 1354.401 kb on + strand at 1354.406 kb on + strand at 1354.409 kb on - strand at 1354.409 kb on - strand at 1354.410 kb on + strand at 1354.410 kb on + strand at 1354.416 kb on - strand at 1354.420 kb on - strand at 1354.420 kb on - strand at 1354.421 kb on + strand at 1354.425 kb on + strand at 1354.425 kb on + strand at 1354.425 kb on + strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.438 kb on - strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.439 kb on + strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.445 kb on - strand at 1354.446 kb on + strand at 1354.447 kb on - strand at 1354.447 kb on - strand at 1354.448 kb on + strand at 1354.448 kb on + strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.449 kb on - strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.450 kb on + strand at 1354.457 kb on - strand at 1354.457 kb on - strand at 1354.457 kb on - strand at 1354.458 kb on + strand at 1354.458 kb on + strand at 1354.458 kb on + strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.459 kb on - strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.460 kb on + strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.463 kb on - strand at 1354.464 kb on + strand at 1354.464 kb on + strand at 1354.464 kb on + strand at 1354.474 kb on - strand at 1354.501 kb on - strand at 1354.502 kb on + strand at 1354.534 kb on + strand at 1354.538 kb on + strand at 1354.544 kb on + strand at 1354.560 kb on + strand at 1354.723 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.828 kb on - strand, within MMJJ_RS07375 at 1354.834 kb on - strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.835 kb on + strand, within MMJJ_RS07375 at 1354.903 kb on - strand, within MMJJ_RS07375 at 1354.907 kb on - strand, within MMJJ_RS07375 at 1355.129 kb on - strand at 1355.150 kb on + strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.191 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.212 kb on - strand, within MMJJ_RS07375 at 1355.213 kb on + strand, within MMJJ_RS07375 at 1355.231 kb on - strand, within MMJJ_RS07375 at 1355.232 kb on + strand, within MMJJ_RS07375 at 1355.250 kb on + strand, within MMJJ_RS07375 at 1355.281 kb on - strand, within MMJJ_RS07375 at 1355.301 kb on - strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.302 kb on + strand, within MMJJ_RS07375 at 1355.304 kb on - strand, within MMJJ_RS07375 at 1355.305 kb on + strand, within MMJJ_RS07375 at 1355.339 kb on - strand, within MMJJ_RS07375
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,352,331 + MMJJ_RS07360 0.83 -3.0 1,352,425 + +0.4 1,352,485 - +1.3 1,352,485 - +1.4 1,352,493 - -0.0 1,352,526 - -0.2 1,352,526 - -0.4 1,352,527 + +1.4 1,352,556 + -0.8 1,352,646 + MMJJ_RS07365 0.21 +0.1 1,352,673 - MMJJ_RS07365 0.25 +1.5 1,352,674 + MMJJ_RS07365 0.25 +1.7 1,352,674 + MMJJ_RS07365 0.25 -0.0 1,352,674 + MMJJ_RS07365 0.25 -0.6 1,352,674 + MMJJ_RS07365 0.25 +0.1 1,352,678 - MMJJ_RS07365 0.25 -0.3 1,352,678 - MMJJ_RS07365 0.25 +0.3 1,352,678 - MMJJ_RS07365 0.25 +0.3 1,352,678 - MMJJ_RS07365 0.25 -0.6 1,352,678 - MMJJ_RS07365 0.25 +1.6 1,352,679 + MMJJ_RS07365 0.26 -0.1 1,352,679 + MMJJ_RS07365 0.26 +0.1 1,352,680 - MMJJ_RS07365 0.26 +0.9 1,352,705 - MMJJ_RS07365 0.29 +0.3 1,352,705 - MMJJ_RS07365 0.29 -0.1 1,352,706 + MMJJ_RS07365 0.30 -2.7 1,352,754 + MMJJ_RS07365 0.37 -0.6 1,352,760 - MMJJ_RS07365 0.37 -0.4 1,352,761 + MMJJ_RS07365 0.38 +0.5 1,352,779 + MMJJ_RS07365 0.40 -0.4 1,352,839 + MMJJ_RS07365 0.49 +0.4 1,352,863 + MMJJ_RS07365 0.53 -0.5 1,352,875 - MMJJ_RS07365 0.54 +0.4 1,352,875 - MMJJ_RS07365 0.54 +1.4 1,352,876 + MMJJ_RS07365 0.54 +1.1 1,352,876 + MMJJ_RS07365 0.54 -0.9 1,352,894 + MMJJ_RS07365 0.57 +0.3 1,352,919 - MMJJ_RS07365 0.61 -0.2 1,352,919 - MMJJ_RS07365 0.61 +0.6 1,352,920 + MMJJ_RS07365 0.61 -0.2 1,352,920 + MMJJ_RS07365 0.61 +0.7 1,352,920 + MMJJ_RS07365 0.61 -0.5 1,352,961 + MMJJ_RS07365 0.67 +1.0 1,352,964 - MMJJ_RS07365 0.67 +1.4 1,353,004 - MMJJ_RS07365 0.73 +0.5 1,353,004 - MMJJ_RS07365 0.73 +1.1 1,353,011 - MMJJ_RS07365 0.74 +1.3 1,353,011 - MMJJ_RS07365 0.74 +0.5 1,353,011 - MMJJ_RS07365 0.74 +0.4 1,353,011 - MMJJ_RS07365 0.74 +0.7 1,353,012 + MMJJ_RS07365 0.74 +0.9 1,353,053 + MMJJ_RS07365 0.80 +0.1 1,353,056 - MMJJ_RS07365 0.81 -0.5 1,353,057 + MMJJ_RS07365 0.81 +0.2 1,353,057 + MMJJ_RS07365 0.81 +0.1 1,353,057 + MMJJ_RS07365 0.81 +0.8 1,353,057 + MMJJ_RS07365 0.81 +0.3 1,353,124 - +0.6 1,353,149 - +0.2 1,353,152 - +0.5 1,353,161 + +1.3 1,353,176 - -0.2 1,353,188 - +0.9 1,353,188 - -0.0 1,353,188 - +0.9 1,353,190 - +0.9 1,353,197 - +0.2 1,353,197 - +1.0 1,353,285 - -1.6 1,353,541 - MMJJ_RS07370 0.30 +0.4 1,353,565 - MMJJ_RS07370 0.32 -1.2 1,353,627 - MMJJ_RS07370 0.37 +0.4 1,353,634 + +1.4 1,353,663 - MMJJ_RS07370 0.40 -3.4 1,353,726 - -0.6 1,353,758 + MMJJ_RS07370 0.48 -0.2 1,353,758 + MMJJ_RS07370 0.48 -2.8 1,353,913 + MMJJ_RS07370 0.62 -1.4 1,353,918 - -0.6 1,354,110 - MMJJ_RS07370 0.79 -1.8 1,354,111 + MMJJ_RS07370 0.79 -1.6 1,354,111 + MMJJ_RS07370 0.79 -1.0 1,354,115 + MMJJ_RS07370 0.79 -2.6 1,354,117 - -0.6 1,354,128 + MMJJ_RS07370 0.80 -1.0 1,354,134 - MMJJ_RS07370 0.81 -3.7 1,354,198 + MMJJ_RS07370 0.87 -1.8 1,354,354 + -3.6 1,354,386 - +0.4 1,354,387 + -3.2 1,354,396 - +1.2 1,354,401 + -1.0 1,354,406 + -1.8 1,354,409 - -1.0 1,354,409 - -0.6 1,354,410 + -3.4 1,354,410 + -3.9 1,354,416 - -0.5 1,354,420 - +0.6 1,354,420 - -0.0 1,354,421 + -1.3 1,354,425 + +1.0 1,354,425 + -0.0 1,354,425 + -2.0 1,354,438 - +0.4 1,354,438 - -0.6 1,354,438 - -1.5 1,354,438 - +0.0 1,354,439 + +0.2 1,354,439 + -0.8 1,354,439 + +0.1 1,354,439 + +1.0 1,354,445 - +0.1 1,354,445 - +0.3 1,354,445 - +1.3 1,354,445 - +0.4 1,354,445 - -0.9 1,354,446 + +1.0 1,354,447 - +0.3 1,354,447 - +0.2 1,354,448 + +0.4 1,354,448 + +0.2 1,354,449 - -1.1 1,354,449 - -0.2 1,354,449 - +0.7 1,354,449 - +0.1 1,354,449 - -1.0 1,354,449 - +0.1 1,354,449 - -0.6 1,354,449 - -0.9 1,354,450 + +0.0 1,354,450 + +0.3 1,354,450 + -0.5 1,354,450 + +0.8 1,354,450 + -0.4 1,354,457 - -0.0 1,354,457 - -0.9 1,354,457 - -2.0 1,354,458 + +0.5 1,354,458 + -0.5 1,354,458 + +1.3 1,354,459 - -0.6 1,354,459 - -1.6 1,354,459 - -0.5 1,354,459 - -2.1 1,354,459 - -0.2 1,354,459 - -0.7 1,354,459 - -2.7 1,354,460 + +0.8 1,354,460 + -0.6 1,354,460 + +0.6 1,354,460 + +0.8 1,354,460 + +0.6 1,354,460 + +0.1 1,354,460 + +1.2 1,354,463 - -1.5 1,354,463 - -0.8 1,354,463 - -0.4 1,354,463 - -0.4 1,354,463 - -1.4 1,354,463 - -1.6 1,354,464 + -0.5 1,354,464 + -1.3 1,354,464 + +0.6 1,354,474 - -1.3 1,354,501 - -2.7 1,354,502 + -2.4 1,354,534 + -2.8 1,354,538 + -3.3 1,354,544 + -1.8 1,354,560 + -0.6 1,354,723 - MMJJ_RS07375 0.24 -2.3 1,354,828 - MMJJ_RS07375 0.35 -1.4 1,354,828 - MMJJ_RS07375 0.35 -3.1 1,354,834 - MMJJ_RS07375 0.35 -2.7 1,354,835 + MMJJ_RS07375 0.35 -0.6 1,354,835 + MMJJ_RS07375 0.35 -0.7 1,354,903 - MMJJ_RS07375 0.42 -1.6 1,354,907 - MMJJ_RS07375 0.43 -2.4 1,355,129 - -0.2 1,355,150 + MMJJ_RS07375 0.68 -3.3 1,355,191 - MMJJ_RS07375 0.72 -4.2 1,355,191 - MMJJ_RS07375 0.72 -3.1 1,355,212 - MMJJ_RS07375 0.74 -3.7 1,355,212 - MMJJ_RS07375 0.74 -3.3 1,355,213 + MMJJ_RS07375 0.74 -4.5 1,355,231 - MMJJ_RS07375 0.76 -1.2 1,355,232 + MMJJ_RS07375 0.76 -3.9 1,355,250 + MMJJ_RS07375 0.78 -1.6 1,355,281 - MMJJ_RS07375 0.81 -4.2 1,355,301 - MMJJ_RS07375 0.83 -3.9 1,355,302 + MMJJ_RS07375 0.83 -1.0 1,355,302 + MMJJ_RS07375 0.83 -2.1 1,355,302 + MMJJ_RS07375 0.83 -1.6 1,355,304 - MMJJ_RS07375 0.83 -2.8 1,355,305 + MMJJ_RS07375 0.83 -2.4 1,355,339 - MMJJ_RS07375 0.87 -3.7
Or see this region's nucleotide sequence