Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04805 and MMJJ_RS04810 are separated by 133 nucleotides MMJJ_RS04810 and MMJJ_RS04815 are separated by 168 nucleotides MMJJ_RS04815 and MMJJ_RS04820 are separated by 33 nucleotides
MMJJ_RS04805: MMJJ_RS04805 - N-acetyl-gamma-glutamyl-phosphate reductase, at 900,427 to 901,458
_RS04805
MMJJ_RS04810: MMJJ_RS04810 - nitroreductase family protein, at 901,592 to 902,113
_RS04810
MMJJ_RS04815: MMJJ_RS04815 - TIM barrel protein, at 902,282 to 902,938
_RS04815
MMJJ_RS04820: MMJJ_RS04820 - hypothetical protein, at 902,972 to 903,781
_RS04820
Position (kb) 901
902
903 Strain fitness (log2 ratio) -2
-1
0
1
2 at 900.874 kb on - strand, within MMJJ_RS04805 at 901.178 kb on + strand, within MMJJ_RS04805 at 901.293 kb on - strand, within MMJJ_RS04805 at 901.499 kb on + strand at 901.526 kb on - strand at 901.527 kb on + strand at 901.533 kb on - strand at 901.563 kb on + strand at 901.599 kb on - strand at 901.620 kb on - strand at 901.625 kb on - strand at 901.686 kb on + strand, within MMJJ_RS04810 at 901.754 kb on - strand, within MMJJ_RS04810 at 901.755 kb on + strand, within MMJJ_RS04810 at 901.761 kb on - strand, within MMJJ_RS04810 at 901.762 kb on + strand, within MMJJ_RS04810 at 901.776 kb on + strand, within MMJJ_RS04810 at 901.805 kb on - strand, within MMJJ_RS04810 at 901.805 kb on - strand at 901.805 kb on - strand, within MMJJ_RS04810 at 901.805 kb on - strand, within MMJJ_RS04810 at 901.808 kb on - strand, within MMJJ_RS04810 at 901.809 kb on + strand, within MMJJ_RS04810 at 901.811 kb on - strand, within MMJJ_RS04810 at 901.811 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.889 kb on - strand, within MMJJ_RS04810 at 901.895 kb on - strand, within MMJJ_RS04810 at 901.896 kb on + strand, within MMJJ_RS04810 at 901.896 kb on + strand, within MMJJ_RS04810 at 901.977 kb on + strand, within MMJJ_RS04810 at 902.048 kb on - strand, within MMJJ_RS04810 at 902.076 kb on - strand at 902.076 kb on - strand at 902.077 kb on + strand at 902.102 kb on - strand at 902.113 kb on - strand at 902.113 kb on - strand at 902.114 kb on + strand at 902.114 kb on + strand at 902.115 kb on - strand at 902.115 kb on - strand at 902.143 kb on + strand at 902.147 kb on + strand at 902.170 kb on - strand at 902.171 kb on + strand at 902.171 kb on + strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.196 kb on - strand at 902.198 kb on - strand at 902.198 kb on - strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.247 kb on + strand at 902.281 kb on - strand at 902.282 kb on + strand at 902.287 kb on + strand at 902.332 kb on - strand at 902.413 kb on + strand, within MMJJ_RS04815 at 902.471 kb on + strand, within MMJJ_RS04815 at 902.479 kb on - strand, within MMJJ_RS04815 at 902.494 kb on + strand, within MMJJ_RS04815 at 902.498 kb on + strand, within MMJJ_RS04815 at 902.567 kb on - strand, within MMJJ_RS04815 at 902.641 kb on + strand, within MMJJ_RS04815 at 902.686 kb on + strand, within MMJJ_RS04815 at 902.759 kb on - strand, within MMJJ_RS04815 at 902.759 kb on - strand, within MMJJ_RS04815 at 902.839 kb on + strand, within MMJJ_RS04815 at 902.872 kb on - strand, within MMJJ_RS04815 at 902.914 kb on - strand at 902.915 kb on + strand at 902.915 kb on + strand at 902.918 kb on - strand at 902.936 kb on - strand at 902.937 kb on + strand at 902.946 kb on - strand at 902.946 kb on - strand at 902.947 kb on + strand at 902.947 kb on + strand at 902.947 kb on + strand at 902.953 kb on + strand at 902.957 kb on - strand at 902.957 kb on - strand at 902.961 kb on - strand at 902.961 kb on - strand at 902.961 kb on - strand at 902.962 kb on + strand at 902.962 kb on + strand at 902.962 kb on + strand at 903.006 kb on - strand at 903.109 kb on - strand, within MMJJ_RS04820
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 900,874 - MMJJ_RS04805 0.43 -1.7 901,178 + MMJJ_RS04805 0.73 -1.2 901,293 - MMJJ_RS04805 0.84 -2.3 901,499 + -1.5 901,526 - -0.9 901,527 + -0.1 901,533 - +0.4 901,563 + -0.1 901,599 - -1.1 901,620 - -0.2 901,625 - -0.2 901,686 + MMJJ_RS04810 0.18 +0.8 901,754 - MMJJ_RS04810 0.31 +0.9 901,755 + MMJJ_RS04810 0.31 -0.2 901,761 - MMJJ_RS04810 0.32 -1.0 901,762 + MMJJ_RS04810 0.33 +0.6 901,776 + MMJJ_RS04810 0.35 +1.1 901,805 - MMJJ_RS04810 0.41 +0.6 901,805 - +0.5 901,805 - MMJJ_RS04810 0.41 -0.4 901,805 - MMJJ_RS04810 0.41 +0.9 901,808 - MMJJ_RS04810 0.41 +0.5 901,809 + MMJJ_RS04810 0.42 +0.8 901,811 - MMJJ_RS04810 0.42 +0.2 901,811 - MMJJ_RS04810 0.42 -0.6 901,889 - MMJJ_RS04810 0.57 -1.5 901,889 - MMJJ_RS04810 0.57 +1.8 901,889 - MMJJ_RS04810 0.57 +0.0 901,895 - MMJJ_RS04810 0.58 -0.1 901,896 + MMJJ_RS04810 0.58 -0.9 901,896 + MMJJ_RS04810 0.58 -0.3 901,977 + MMJJ_RS04810 0.74 +0.1 902,048 - MMJJ_RS04810 0.87 +0.7 902,076 - -0.8 902,076 - -0.2 902,077 + +0.2 902,102 - +1.1 902,113 - -0.7 902,113 - +0.4 902,114 + -0.0 902,114 + +0.4 902,115 - +1.1 902,115 - -0.3 902,143 + -0.9 902,147 + +1.0 902,170 - +0.8 902,171 + +0.6 902,171 + +0.5 902,172 - -1.4 902,172 - -0.8 902,172 - +0.5 902,196 - +0.7 902,198 - +0.6 902,198 - +0.7 902,199 + +1.8 902,199 + +1.2 902,199 + -0.9 902,199 + -0.4 902,247 + +0.5 902,281 - +0.1 902,282 + -0.1 902,287 + +0.3 902,332 - -0.1 902,413 + MMJJ_RS04815 0.20 +0.5 902,471 + MMJJ_RS04815 0.29 -0.0 902,479 - MMJJ_RS04815 0.30 -1.5 902,494 + MMJJ_RS04815 0.32 -0.7 902,498 + MMJJ_RS04815 0.33 -0.1 902,567 - MMJJ_RS04815 0.43 +0.8 902,641 + MMJJ_RS04815 0.55 +0.6 902,686 + MMJJ_RS04815 0.61 -1.1 902,759 - MMJJ_RS04815 0.73 -1.0 902,759 - MMJJ_RS04815 0.73 +0.5 902,839 + MMJJ_RS04815 0.85 -0.0 902,872 - MMJJ_RS04815 0.90 +0.2 902,914 - +0.4 902,915 + +0.1 902,915 + -0.3 902,918 - -2.2 902,936 - -1.4 902,937 + -0.9 902,946 - -1.1 902,946 - -0.1 902,947 + -0.0 902,947 + +0.8 902,947 + -1.0 902,953 + +0.1 902,957 - -0.6 902,957 - -0.8 902,961 - -0.6 902,961 - -0.2 902,961 - +0.4 902,962 + -0.7 902,962 + -0.5 902,962 + +0.9 903,006 - +0.8 903,109 - MMJJ_RS04820 0.17 +0.4
Or see this region's nucleotide sequence