Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03470 and MMJJ_RS03475 are separated by 21 nucleotides MMJJ_RS03475 and MMJJ_RS03480 are separated by 8 nucleotides MMJJ_RS03480 and MMJJ_RS03485 are separated by 213 nucleotides
MMJJ_RS03470: MMJJ_RS03470 - N-6 DNA methylase, at 650,469 to 651,977
_RS03470
MMJJ_RS03475: MMJJ_RS03475 - tyrosine-type recombinase/integrase, at 651,999 to 652,886
_RS03475
MMJJ_RS03480: MMJJ_RS03480 - hypothetical protein, at 652,895 to 653,443
_RS03480
MMJJ_RS03485: MMJJ_RS03485 - radical SAM protein, at 653,657 to 655,030
_RS03485
Position (kb) 652
653
654 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 652.007 kb on - strand at 652.007 kb on - strand at 652.016 kb on - strand at 652.027 kb on - strand at 652.064 kb on + strand at 652.076 kb on - strand at 652.077 kb on + strand at 652.155 kb on + strand, within MMJJ_RS03475 at 652.175 kb on - strand, within MMJJ_RS03475 at 652.212 kb on - strand, within MMJJ_RS03475 at 652.216 kb on - strand, within MMJJ_RS03475 at 652.233 kb on - strand, within MMJJ_RS03475 at 652.318 kb on - strand, within MMJJ_RS03475 at 652.318 kb on - strand, within MMJJ_RS03475 at 652.335 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.356 kb on - strand, within MMJJ_RS03475 at 652.357 kb on + strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.360 kb on - strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.361 kb on + strand, within MMJJ_RS03475 at 652.364 kb on - strand, within MMJJ_RS03475 at 652.417 kb on + strand, within MMJJ_RS03475 at 652.425 kb on - strand, within MMJJ_RS03475 at 652.425 kb on - strand, within MMJJ_RS03475 at 652.426 kb on + strand, within MMJJ_RS03475 at 652.431 kb on - strand, within MMJJ_RS03475 at 652.476 kb on - strand, within MMJJ_RS03475 at 652.483 kb on - strand, within MMJJ_RS03475 at 652.484 kb on + strand, within MMJJ_RS03475 at 652.489 kb on - strand, within MMJJ_RS03475 at 652.492 kb on - strand, within MMJJ_RS03475 at 652.492 kb on - strand, within MMJJ_RS03475 at 652.498 kb on - strand, within MMJJ_RS03475 at 652.507 kb on + strand, within MMJJ_RS03475 at 652.637 kb on + strand, within MMJJ_RS03475 at 652.709 kb on - strand, within MMJJ_RS03475 at 652.709 kb on - strand, within MMJJ_RS03475 at 652.710 kb on + strand, within MMJJ_RS03475 at 652.711 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.720 kb on - strand, within MMJJ_RS03475 at 652.721 kb on + strand, within MMJJ_RS03475 at 652.721 kb on + strand, within MMJJ_RS03475 at 652.728 kb on - strand, within MMJJ_RS03475 at 652.740 kb on - strand, within MMJJ_RS03475 at 652.741 kb on + strand, within MMJJ_RS03475 at 652.749 kb on + strand, within MMJJ_RS03475 at 652.805 kb on - strand at 652.806 kb on + strand at 652.810 kb on - strand at 652.816 kb on + strand at 652.816 kb on + strand at 652.889 kb on - strand at 652.910 kb on - strand at 652.910 kb on - strand at 652.910 kb on - strand at 652.940 kb on - strand at 652.940 kb on - strand at 652.940 kb on - strand at 652.941 kb on + strand at 652.941 kb on + strand at 652.941 kb on + strand at 652.948 kb on - strand at 652.954 kb on - strand, within MMJJ_RS03480 at 652.961 kb on - strand, within MMJJ_RS03480 at 652.961 kb on - strand, within MMJJ_RS03480 at 652.962 kb on + strand, within MMJJ_RS03480 at 652.988 kb on - strand, within MMJJ_RS03480 at 652.988 kb on - strand, within MMJJ_RS03480 at 652.989 kb on + strand, within MMJJ_RS03480 at 653.008 kb on - strand, within MMJJ_RS03480 at 653.009 kb on + strand, within MMJJ_RS03480 at 653.009 kb on + strand, within MMJJ_RS03480 at 653.023 kb on - strand, within MMJJ_RS03480 at 653.024 kb on + strand, within MMJJ_RS03480 at 653.024 kb on + strand, within MMJJ_RS03480 at 653.025 kb on - strand, within MMJJ_RS03480 at 653.066 kb on - strand, within MMJJ_RS03480 at 653.067 kb on + strand, within MMJJ_RS03480 at 653.069 kb on - strand, within MMJJ_RS03480 at 653.070 kb on + strand, within MMJJ_RS03480 at 653.099 kb on - strand, within MMJJ_RS03480 at 653.099 kb on - strand, within MMJJ_RS03480 at 653.100 kb on + strand, within MMJJ_RS03480 at 653.100 kb on + strand, within MMJJ_RS03480 at 653.113 kb on - strand, within MMJJ_RS03480 at 653.114 kb on + strand, within MMJJ_RS03480 at 653.121 kb on + strand, within MMJJ_RS03480 at 653.124 kb on - strand, within MMJJ_RS03480 at 653.142 kb on - strand, within MMJJ_RS03480 at 653.142 kb on - strand, within MMJJ_RS03480 at 653.142 kb on - strand, within MMJJ_RS03480 at 653.142 kb on - strand, within MMJJ_RS03480 at 653.143 kb on + strand, within MMJJ_RS03480 at 653.143 kb on + strand, within MMJJ_RS03480 at 653.146 kb on - strand, within MMJJ_RS03480 at 653.146 kb on - strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.147 kb on + strand, within MMJJ_RS03480 at 653.169 kb on - strand, within MMJJ_RS03480 at 653.170 kb on + strand, within MMJJ_RS03480 at 653.180 kb on - strand, within MMJJ_RS03480 at 653.181 kb on + strand, within MMJJ_RS03480 at 653.181 kb on + strand, within MMJJ_RS03480 at 653.184 kb on - strand, within MMJJ_RS03480 at 653.192 kb on - strand, within MMJJ_RS03480 at 653.192 kb on - strand, within MMJJ_RS03480 at 653.192 kb on - strand, within MMJJ_RS03480 at 653.193 kb on + strand, within MMJJ_RS03480 at 653.201 kb on - strand, within MMJJ_RS03480 at 653.212 kb on - strand, within MMJJ_RS03480 at 653.212 kb on - strand, within MMJJ_RS03480 at 653.212 kb on - strand, within MMJJ_RS03480 at 653.212 kb on - strand, within MMJJ_RS03480 at 653.212 kb on - strand, within MMJJ_RS03480 at 653.317 kb on - strand, within MMJJ_RS03480 at 653.318 kb on + strand, within MMJJ_RS03480 at 653.321 kb on - strand, within MMJJ_RS03480 at 653.321 kb on - strand, within MMJJ_RS03480 at 653.322 kb on + strand, within MMJJ_RS03480 at 653.322 kb on + strand, within MMJJ_RS03480 at 653.322 kb on + strand, within MMJJ_RS03480 at 653.398 kb on - strand at 653.398 kb on - strand at 653.398 kb on - strand at 653.399 kb on + strand at 653.416 kb on - strand at 653.417 kb on + strand at 653.417 kb on + strand at 653.417 kb on + strand at 653.417 kb on + strand at 653.421 kb on - strand at 653.421 kb on - strand at 653.421 kb on - strand at 653.421 kb on - strand at 653.422 kb on + strand at 653.423 kb on - strand at 653.424 kb on + strand at 653.424 kb on + strand at 653.424 kb on + strand at 653.457 kb on - strand at 653.457 kb on - strand at 653.458 kb on + strand at 653.459 kb on - strand at 653.459 kb on - strand at 653.459 kb on - strand at 653.459 kb on - strand at 653.460 kb on + strand at 653.460 kb on + strand at 653.460 kb on + strand at 653.475 kb on - strand at 653.479 kb on + strand at 653.481 kb on + strand at 653.497 kb on + strand at 653.504 kb on - strand at 653.506 kb on - strand at 653.506 kb on - strand at 653.519 kb on - strand at 653.520 kb on + strand at 653.521 kb on - strand at 653.521 kb on - strand at 653.523 kb on - strand at 653.567 kb on - strand at 653.568 kb on + strand at 653.571 kb on + strand at 653.574 kb on + strand at 653.574 kb on + strand at 653.600 kb on + strand at 653.622 kb on - strand at 653.634 kb on - strand at 653.634 kb on - strand at 653.635 kb on + strand at 653.637 kb on - strand at 653.663 kb on - strand at 653.694 kb on + strand at 653.714 kb on - strand at 653.749 kb on - strand at 653.750 kb on + strand at 653.760 kb on + strand at 653.789 kb on - strand at 653.789 kb on - strand at 653.790 kb on + strand at 653.790 kb on + strand at 653.790 kb on + strand at 653.852 kb on + strand, within MMJJ_RS03485 at 653.912 kb on - strand, within MMJJ_RS03485 at 653.947 kb on - strand, within MMJJ_RS03485 at 654.030 kb on + strand, within MMJJ_RS03485 at 654.030 kb on + strand, within MMJJ_RS03485 at 654.035 kb on - strand, within MMJJ_RS03485 at 654.048 kb on + strand, within MMJJ_RS03485 at 654.153 kb on - strand, within MMJJ_RS03485 at 654.154 kb on + strand, within MMJJ_RS03485 at 654.164 kb on - strand, within MMJJ_RS03485 at 654.165 kb on + strand, within MMJJ_RS03485 at 654.204 kb on + strand, within MMJJ_RS03485 at 654.207 kb on - strand, within MMJJ_RS03485 at 654.207 kb on - strand, within MMJJ_RS03485 at 654.208 kb on + strand, within MMJJ_RS03485 at 654.401 kb on - strand, within MMJJ_RS03485 at 654.423 kb on + strand, within MMJJ_RS03485 at 654.423 kb on + strand, within MMJJ_RS03485 at 654.441 kb on - strand, within MMJJ_RS03485 at 654.441 kb on - strand, within MMJJ_RS03485 at 654.442 kb on + strand, within MMJJ_RS03485 at 654.442 kb on + strand, within MMJJ_RS03485 at 654.443 kb on - strand, within MMJJ_RS03485 at 654.443 kb on - strand, within MMJJ_RS03485
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 652,007 - +0.5 652,007 - -0.2 652,016 - +0.1 652,027 - +0.2 652,064 + -0.7 652,076 - +1.5 652,077 + +0.5 652,155 + MMJJ_RS03475 0.18 +0.3 652,175 - MMJJ_RS03475 0.20 +1.0 652,212 - MMJJ_RS03475 0.24 +0.9 652,216 - MMJJ_RS03475 0.24 +0.7 652,233 - MMJJ_RS03475 0.26 +0.2 652,318 - MMJJ_RS03475 0.36 +0.7 652,318 - MMJJ_RS03475 0.36 +0.1 652,335 - MMJJ_RS03475 0.38 -0.2 652,356 - MMJJ_RS03475 0.40 -0.3 652,356 - MMJJ_RS03475 0.40 +0.4 652,356 - MMJJ_RS03475 0.40 -0.1 652,357 + MMJJ_RS03475 0.40 -0.9 652,360 - MMJJ_RS03475 0.41 +0.8 652,360 - MMJJ_RS03475 0.41 +0.8 652,360 - MMJJ_RS03475 0.41 +1.2 652,360 - MMJJ_RS03475 0.41 +0.3 652,361 + MMJJ_RS03475 0.41 -0.5 652,361 + MMJJ_RS03475 0.41 -1.0 652,361 + MMJJ_RS03475 0.41 -0.0 652,361 + MMJJ_RS03475 0.41 +0.2 652,361 + MMJJ_RS03475 0.41 +0.8 652,361 + MMJJ_RS03475 0.41 -0.6 652,361 + MMJJ_RS03475 0.41 +1.0 652,364 - MMJJ_RS03475 0.41 +0.5 652,417 + MMJJ_RS03475 0.47 -0.1 652,425 - MMJJ_RS03475 0.48 +0.4 652,425 - MMJJ_RS03475 0.48 +1.0 652,426 + MMJJ_RS03475 0.48 +1.3 652,431 - MMJJ_RS03475 0.49 +1.0 652,476 - MMJJ_RS03475 0.54 -0.7 652,483 - MMJJ_RS03475 0.55 +0.0 652,484 + MMJJ_RS03475 0.55 -0.0 652,489 - MMJJ_RS03475 0.55 +0.4 652,492 - MMJJ_RS03475 0.56 -1.0 652,492 - MMJJ_RS03475 0.56 +0.6 652,498 - MMJJ_RS03475 0.56 -0.7 652,507 + MMJJ_RS03475 0.57 -0.6 652,637 + MMJJ_RS03475 0.72 -0.4 652,709 - MMJJ_RS03475 0.80 +0.0 652,709 - MMJJ_RS03475 0.80 +1.1 652,710 + MMJJ_RS03475 0.80 -1.1 652,711 - MMJJ_RS03475 0.80 +1.2 652,720 - MMJJ_RS03475 0.81 -0.7 652,720 - MMJJ_RS03475 0.81 +0.1 652,720 - MMJJ_RS03475 0.81 +0.7 652,721 + MMJJ_RS03475 0.81 -0.1 652,721 + MMJJ_RS03475 0.81 -0.5 652,728 - MMJJ_RS03475 0.82 -0.6 652,740 - MMJJ_RS03475 0.83 -2.4 652,741 + MMJJ_RS03475 0.84 -0.9 652,749 + MMJJ_RS03475 0.84 +1.0 652,805 - +0.2 652,806 + +1.0 652,810 - +0.3 652,816 + -0.5 652,816 + +0.4 652,889 - +1.2 652,910 - -0.1 652,910 - -0.1 652,910 - +0.1 652,940 - +0.4 652,940 - +1.4 652,940 - +1.1 652,941 + +0.5 652,941 + -0.1 652,941 + +0.9 652,948 - -0.1 652,954 - MMJJ_RS03480 0.11 +0.7 652,961 - MMJJ_RS03480 0.12 -1.5 652,961 - MMJJ_RS03480 0.12 -0.4 652,962 + MMJJ_RS03480 0.12 +0.2 652,988 - MMJJ_RS03480 0.17 +0.1 652,988 - MMJJ_RS03480 0.17 -0.4 652,989 + MMJJ_RS03480 0.17 -0.3 653,008 - MMJJ_RS03480 0.21 -0.3 653,009 + MMJJ_RS03480 0.21 -0.1 653,009 + MMJJ_RS03480 0.21 -0.1 653,023 - MMJJ_RS03480 0.23 +0.2 653,024 + MMJJ_RS03480 0.23 +0.2 653,024 + MMJJ_RS03480 0.23 +0.4 653,025 - MMJJ_RS03480 0.24 +1.2 653,066 - MMJJ_RS03480 0.31 +1.1 653,067 + MMJJ_RS03480 0.31 +0.6 653,069 - MMJJ_RS03480 0.32 +0.2 653,070 + MMJJ_RS03480 0.32 -0.2 653,099 - MMJJ_RS03480 0.37 +0.5 653,099 - MMJJ_RS03480 0.37 +0.9 653,100 + MMJJ_RS03480 0.37 -0.5 653,100 + MMJJ_RS03480 0.37 -0.2 653,113 - MMJJ_RS03480 0.40 +0.1 653,114 + MMJJ_RS03480 0.40 +1.1 653,121 + MMJJ_RS03480 0.41 -0.5 653,124 - MMJJ_RS03480 0.42 +0.5 653,142 - MMJJ_RS03480 0.45 +1.3 653,142 - MMJJ_RS03480 0.45 -0.3 653,142 - MMJJ_RS03480 0.45 +1.3 653,142 - MMJJ_RS03480 0.45 +0.3 653,143 + MMJJ_RS03480 0.45 +1.0 653,143 + MMJJ_RS03480 0.45 +1.4 653,146 - MMJJ_RS03480 0.46 -0.5 653,146 - MMJJ_RS03480 0.46 -2.0 653,147 + MMJJ_RS03480 0.46 +0.4 653,147 + MMJJ_RS03480 0.46 +0.4 653,147 + MMJJ_RS03480 0.46 -0.9 653,147 + MMJJ_RS03480 0.46 +0.2 653,147 + MMJJ_RS03480 0.46 -0.6 653,147 + MMJJ_RS03480 0.46 +0.0 653,147 + MMJJ_RS03480 0.46 +0.6 653,169 - MMJJ_RS03480 0.50 +0.0 653,170 + MMJJ_RS03480 0.50 +1.5 653,180 - MMJJ_RS03480 0.52 -0.8 653,181 + MMJJ_RS03480 0.52 +0.1 653,181 + MMJJ_RS03480 0.52 -0.4 653,184 - MMJJ_RS03480 0.53 -1.1 653,192 - MMJJ_RS03480 0.54 +0.0 653,192 - MMJJ_RS03480 0.54 -0.2 653,192 - MMJJ_RS03480 0.54 +0.3 653,193 + MMJJ_RS03480 0.54 -0.6 653,201 - MMJJ_RS03480 0.56 -0.2 653,212 - MMJJ_RS03480 0.58 -0.7 653,212 - MMJJ_RS03480 0.58 +0.4 653,212 - MMJJ_RS03480 0.58 -1.2 653,212 - MMJJ_RS03480 0.58 +0.5 653,212 - MMJJ_RS03480 0.58 +1.1 653,317 - MMJJ_RS03480 0.77 -0.1 653,318 + MMJJ_RS03480 0.77 +1.0 653,321 - MMJJ_RS03480 0.78 +0.1 653,321 - MMJJ_RS03480 0.78 -0.1 653,322 + MMJJ_RS03480 0.78 -0.3 653,322 + MMJJ_RS03480 0.78 +0.1 653,322 + MMJJ_RS03480 0.78 +1.3 653,398 - -1.0 653,398 - +0.3 653,398 - +0.6 653,399 + +1.8 653,416 - -0.8 653,417 + +0.3 653,417 + -0.5 653,417 + -1.2 653,417 + -0.6 653,421 - +1.1 653,421 - +0.7 653,421 - +0.1 653,421 - -1.2 653,422 + +0.8 653,423 - -0.1 653,424 + -0.9 653,424 + +0.6 653,424 + -0.4 653,457 - -3.0 653,457 - +0.7 653,458 + -0.1 653,459 - +0.1 653,459 - -0.3 653,459 - +1.3 653,459 - +0.3 653,460 + -0.0 653,460 + -0.1 653,460 + -1.0 653,475 - +0.7 653,479 + +0.9 653,481 + -0.3 653,497 + +0.6 653,504 - +0.3 653,506 - -0.1 653,506 - -1.1 653,519 - +0.7 653,520 + +0.9 653,521 - -0.2 653,521 - +0.2 653,523 - -0.3 653,567 - -0.6 653,568 + -0.1 653,571 + -0.8 653,574 + +0.7 653,574 + -0.3 653,600 + -0.1 653,622 - +1.3 653,634 - +0.1 653,634 - +1.0 653,635 + -0.4 653,637 - +1.1 653,663 - +0.2 653,694 + -0.7 653,714 - -0.4 653,749 - -0.1 653,750 + +0.4 653,760 + +0.0 653,789 - -0.2 653,789 - +0.5 653,790 + -0.6 653,790 + +1.5 653,790 + +0.8 653,852 + MMJJ_RS03485 0.14 -0.8 653,912 - MMJJ_RS03485 0.19 +0.8 653,947 - MMJJ_RS03485 0.21 -0.1 654,030 + MMJJ_RS03485 0.27 +0.4 654,030 + MMJJ_RS03485 0.27 +0.6 654,035 - MMJJ_RS03485 0.28 +0.4 654,048 + MMJJ_RS03485 0.28 +1.3 654,153 - MMJJ_RS03485 0.36 +0.7 654,154 + MMJJ_RS03485 0.36 +0.1 654,164 - MMJJ_RS03485 0.37 +0.4 654,165 + MMJJ_RS03485 0.37 -0.5 654,204 + MMJJ_RS03485 0.40 +0.2 654,207 - MMJJ_RS03485 0.40 -0.7 654,207 - MMJJ_RS03485 0.40 -1.4 654,208 + MMJJ_RS03485 0.40 -0.6 654,401 - MMJJ_RS03485 0.54 +0.3 654,423 + MMJJ_RS03485 0.56 +0.3 654,423 + MMJJ_RS03485 0.56 -0.2 654,441 - MMJJ_RS03485 0.57 -1.2 654,441 - MMJJ_RS03485 0.57 +0.7 654,442 + MMJJ_RS03485 0.57 +0.1 654,442 + MMJJ_RS03485 0.57 -0.2 654,443 - MMJJ_RS03485 0.57 +0.1 654,443 - MMJJ_RS03485 0.57 +0.6
Or see this region's nucleotide sequence