Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03215 and MMJJ_RS03220 are separated by 65 nucleotides MMJJ_RS03220 and MMJJ_RS03225 are separated by 86 nucleotides MMJJ_RS03225 and MMJJ_RS03230 are separated by 2 nucleotides MMJJ_RS03230 and MMJJ_RS03235 are separated by 32 nucleotides MMJJ_RS03235 and MMJJ_RS03240 are separated by 289 nucleotides
MMJJ_RS03215: MMJJ_RS03215 - alanine--glyoxylate aminotransferase family protein, at 609,411 to 610,559
_RS03215
MMJJ_RS03220: MMJJ_RS03220 - inosine/xanthosine triphosphatase, at 610,625 to 611,284
_RS03220
MMJJ_RS03225: MMJJ_RS03225 - 4Fe-4S binding protein, at 611,371 to 611,535
_RS03225
MMJJ_RS03230: MMJJ_RS03230 - NAD(P)/FAD-dependent oxidoreductase, at 611,538 to 612,710
_RS03230
MMJJ_RS03235: MMJJ_RS03235 - hypothetical protein, at 612,743 to 613,264
_RS03235
MMJJ_RS03240: MMJJ_RS03240 - histone family protein, at 613,554 to 613,757
_RS03240
Position (kb) 611
612
613 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 610.550 kb on + strand at 610.557 kb on - strand at 610.565 kb on + strand at 610.581 kb on - strand at 610.600 kb on + strand at 610.604 kb on - strand at 610.605 kb on + strand at 610.605 kb on + strand at 610.623 kb on - strand at 610.624 kb on + strand at 610.690 kb on + strand at 610.690 kb on + strand at 610.766 kb on + strand, within MMJJ_RS03220 at 610.845 kb on - strand, within MMJJ_RS03220 at 610.845 kb on - strand, within MMJJ_RS03220 at 610.846 kb on + strand, within MMJJ_RS03220 at 610.846 kb on + strand, within MMJJ_RS03220 at 610.907 kb on + strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.916 kb on - strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.917 kb on + strand, within MMJJ_RS03220 at 610.918 kb on - strand, within MMJJ_RS03220 at 610.948 kb on + strand, within MMJJ_RS03220 at 611.028 kb on - strand, within MMJJ_RS03220 at 611.028 kb on - strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.029 kb on + strand, within MMJJ_RS03220 at 611.030 kb on - strand, within MMJJ_RS03220 at 611.030 kb on - strand, within MMJJ_RS03220 at 611.031 kb on + strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.032 kb on - strand, within MMJJ_RS03220 at 611.033 kb on + strand, within MMJJ_RS03220 at 611.033 kb on + strand, within MMJJ_RS03220 at 611.086 kb on - strand, within MMJJ_RS03220 at 611.188 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.200 kb on - strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.201 kb on + strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.202 kb on - strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.203 kb on + strand, within MMJJ_RS03220 at 611.207 kb on + strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.217 kb on - strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.218 kb on + strand, within MMJJ_RS03220 at 611.221 kb on - strand at 611.235 kb on - strand at 611.235 kb on - strand at 611.236 kb on + strand at 611.255 kb on - strand at 611.256 kb on + strand at 611.275 kb on + strand at 611.282 kb on - strand at 611.292 kb on - strand at 611.295 kb on - strand at 611.295 kb on - strand at 611.296 kb on + strand at 611.296 kb on + strand at 611.317 kb on - strand at 611.318 kb on + strand at 611.318 kb on + strand at 611.321 kb on - strand at 611.321 kb on - strand at 611.321 kb on - strand at 611.324 kb on + strand at 611.324 kb on + strand at 611.324 kb on + strand at 611.342 kb on + strand at 611.420 kb on - strand, within MMJJ_RS03225 at 611.425 kb on + strand, within MMJJ_RS03225 at 611.499 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.507 kb on - strand, within MMJJ_RS03225 at 611.560 kb on + strand at 611.641 kb on - strand at 612.012 kb on + strand, within MMJJ_RS03230 at 612.153 kb on - strand, within MMJJ_RS03230 at 612.180 kb on + strand, within MMJJ_RS03230 at 612.191 kb on + strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.235 kb on - strand, within MMJJ_RS03230 at 612.236 kb on + strand, within MMJJ_RS03230 at 612.385 kb on - strand, within MMJJ_RS03230 at 612.410 kb on + strand, within MMJJ_RS03230 at 612.477 kb on - strand, within MMJJ_RS03230 at 612.634 kb on - strand at 612.635 kb on + strand at 612.635 kb on + strand at 612.635 kb on + strand at 612.657 kb on + strand at 612.661 kb on - strand at 612.704 kb on + strand at 612.721 kb on - strand at 612.721 kb on - strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.722 kb on + strand at 612.737 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.780 kb on - strand at 612.781 kb on + strand at 612.783 kb on - strand at 612.783 kb on - strand at 612.784 kb on + strand at 612.784 kb on + strand at 612.784 kb on + strand at 612.792 kb on + strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.794 kb on - strand at 612.812 kb on - strand, within MMJJ_RS03235 at 612.812 kb on - strand, within MMJJ_RS03235 at 612.813 kb on + strand, within MMJJ_RS03235 at 612.814 kb on - strand, within MMJJ_RS03235 at 612.815 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.819 kb on + strand, within MMJJ_RS03235 at 612.836 kb on - strand, within MMJJ_RS03235 at 612.864 kb on + strand, within MMJJ_RS03235 at 613.056 kb on - strand, within MMJJ_RS03235 at 613.348 kb on - strand at 613.400 kb on + strand at 613.403 kb on + strand at 613.405 kb on - strand at 613.406 kb on + strand at 613.408 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.410 kb on - strand at 613.411 kb on + strand at 613.411 kb on + strand at 613.415 kb on + strand at 613.415 kb on + strand at 613.420 kb on + strand at 613.422 kb on - strand at 613.425 kb on + strand at 613.425 kb on + strand at 613.429 kb on - strand at 613.430 kb on + strand at 613.430 kb on + strand at 613.449 kb on + strand at 613.449 kb on + strand at 613.469 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.507 kb on + strand at 613.553 kb on - strand at 613.565 kb on + strand at 613.582 kb on - strand, within MMJJ_RS03240 at 613.588 kb on - strand, within MMJJ_RS03240 at 613.616 kb on + strand, within MMJJ_RS03240
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 610,550 + -0.4 610,557 - +0.4 610,565 + -0.1 610,581 - -1.4 610,600 + -1.7 610,604 - +0.0 610,605 + +1.2 610,605 + +0.1 610,623 - -0.1 610,624 + +0.7 610,690 + +0.6 610,690 + -0.2 610,766 + MMJJ_RS03220 0.21 +0.6 610,845 - MMJJ_RS03220 0.33 -0.1 610,845 - MMJJ_RS03220 0.33 +0.1 610,846 + MMJJ_RS03220 0.33 +1.3 610,846 + MMJJ_RS03220 0.33 -0.1 610,907 + MMJJ_RS03220 0.43 -0.0 610,916 - MMJJ_RS03220 0.44 -0.2 610,916 - MMJJ_RS03220 0.44 +1.2 610,916 - MMJJ_RS03220 0.44 -0.6 610,916 - MMJJ_RS03220 0.44 +0.3 610,917 + MMJJ_RS03220 0.44 -0.2 610,917 + MMJJ_RS03220 0.44 -0.2 610,917 + MMJJ_RS03220 0.44 +0.8 610,918 - MMJJ_RS03220 0.44 -0.6 610,948 + MMJJ_RS03220 0.49 -0.4 611,028 - MMJJ_RS03220 0.61 -0.2 611,028 - MMJJ_RS03220 0.61 -0.8 611,029 + MMJJ_RS03220 0.61 +0.1 611,029 + MMJJ_RS03220 0.61 -0.2 611,029 + MMJJ_RS03220 0.61 -0.6 611,029 + MMJJ_RS03220 0.61 -0.3 611,030 - MMJJ_RS03220 0.61 -0.3 611,030 - MMJJ_RS03220 0.61 -0.1 611,031 + MMJJ_RS03220 0.62 -0.9 611,032 - MMJJ_RS03220 0.62 -1.1 611,032 - MMJJ_RS03220 0.62 -2.6 611,032 - MMJJ_RS03220 0.62 -0.5 611,033 + MMJJ_RS03220 0.62 +1.1 611,033 + MMJJ_RS03220 0.62 -0.1 611,086 - MMJJ_RS03220 0.70 -0.0 611,188 - MMJJ_RS03220 0.85 -0.4 611,200 - MMJJ_RS03220 0.87 -1.5 611,200 - MMJJ_RS03220 0.87 -0.4 611,200 - MMJJ_RS03220 0.87 -1.0 611,200 - MMJJ_RS03220 0.87 +1.0 611,201 + MMJJ_RS03220 0.87 +0.4 611,201 + MMJJ_RS03220 0.87 -0.7 611,201 + MMJJ_RS03220 0.87 +0.0 611,201 + MMJJ_RS03220 0.87 +1.0 611,201 + MMJJ_RS03220 0.87 +0.7 611,202 - MMJJ_RS03220 0.87 -0.2 611,202 - MMJJ_RS03220 0.87 -0.1 611,202 - MMJJ_RS03220 0.87 +1.0 611,202 - MMJJ_RS03220 0.87 +0.2 611,202 - MMJJ_RS03220 0.87 -0.1 611,203 + MMJJ_RS03220 0.88 -0.4 611,203 + MMJJ_RS03220 0.88 -0.4 611,203 + MMJJ_RS03220 0.88 +0.0 611,207 + MMJJ_RS03220 0.88 -1.3 611,217 - MMJJ_RS03220 0.90 -0.0 611,217 - MMJJ_RS03220 0.90 -0.6 611,217 - MMJJ_RS03220 0.90 -0.8 611,217 - MMJJ_RS03220 0.90 +0.4 611,218 + MMJJ_RS03220 0.90 +0.8 611,218 + MMJJ_RS03220 0.90 +1.1 611,218 + MMJJ_RS03220 0.90 -3.6 611,218 + MMJJ_RS03220 0.90 -0.4 611,221 - -0.4 611,235 - -0.6 611,235 - +0.7 611,236 + -0.1 611,255 - -0.2 611,256 + +0.2 611,275 + +0.2 611,282 - -0.9 611,292 - -0.5 611,295 - -0.9 611,295 - -0.6 611,296 + -1.0 611,296 + -1.9 611,317 - -1.9 611,318 + -1.3 611,318 + -1.3 611,321 - -1.9 611,321 - -1.9 611,321 - -1.3 611,324 + -3.1 611,324 + -2.6 611,324 + -2.6 611,342 + -2.4 611,420 - MMJJ_RS03225 0.30 -1.3 611,425 + MMJJ_RS03225 0.33 -2.3 611,499 - MMJJ_RS03225 0.78 -2.6 611,507 - MMJJ_RS03225 0.82 -0.9 611,507 - MMJJ_RS03225 0.82 -2.4 611,507 - MMJJ_RS03225 0.82 -0.5 611,560 + -1.7 611,641 - -0.5 612,012 + MMJJ_RS03230 0.40 -0.9 612,153 - MMJJ_RS03230 0.52 -1.7 612,180 + MMJJ_RS03230 0.55 -2.7 612,191 + MMJJ_RS03230 0.56 -1.7 612,235 - MMJJ_RS03230 0.59 -2.4 612,235 - MMJJ_RS03230 0.59 -2.3 612,236 + MMJJ_RS03230 0.60 -2.4 612,385 - MMJJ_RS03230 0.72 -3.3 612,410 + MMJJ_RS03230 0.74 -3.3 612,477 - MMJJ_RS03230 0.80 -4.3 612,634 - -2.3 612,635 + -3.8 612,635 + -3.3 612,635 + -2.3 612,657 + -3.4 612,661 - -1.7 612,704 + -0.6 612,721 - +1.1 612,721 - -0.1 612,722 + +0.1 612,722 + +0.8 612,722 + -1.2 612,722 + -0.2 612,737 - +0.7 612,780 - -0.0 612,780 - +0.5 612,780 - -1.2 612,780 - +0.1 612,781 + +0.6 612,783 - +0.0 612,783 - -0.5 612,784 + +0.4 612,784 + +0.3 612,784 + +0.1 612,792 + +0.7 612,794 - -0.5 612,794 - +0.4 612,794 - -0.3 612,794 - -0.4 612,812 - MMJJ_RS03235 0.13 +0.5 612,812 - MMJJ_RS03235 0.13 -0.5 612,813 + MMJJ_RS03235 0.13 +0.6 612,814 - MMJJ_RS03235 0.14 -1.0 612,815 + MMJJ_RS03235 0.14 +0.5 612,819 + MMJJ_RS03235 0.15 +0.6 612,819 + MMJJ_RS03235 0.15 +0.3 612,819 + MMJJ_RS03235 0.15 +0.5 612,836 - MMJJ_RS03235 0.18 +0.7 612,864 + MMJJ_RS03235 0.23 -1.3 613,056 - MMJJ_RS03235 0.60 -2.7 613,348 - +0.1 613,400 + +1.6 613,403 + -2.4 613,405 - +0.3 613,406 + +0.0 613,408 - -0.3 613,410 - -0.3 613,410 - -0.1 613,410 - -0.1 613,411 + -0.9 613,411 + -1.3 613,415 + -2.3 613,415 + -0.9 613,420 + -1.7 613,422 - -0.1 613,425 + -0.3 613,425 + -0.7 613,429 - -2.4 613,430 + -0.0 613,430 + +0.4 613,449 + +0.1 613,449 + +0.3 613,469 + +0.7 613,507 + -0.4 613,507 + +0.7 613,507 + +0.1 613,553 - +0.2 613,565 + +0.5 613,582 - MMJJ_RS03240 0.14 +0.2 613,588 - MMJJ_RS03240 0.17 -3.8 613,616 + MMJJ_RS03240 0.30 -0.1
Or see this region's nucleotide sequence