Experiment: R2A with arsenate 15.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS06865 and LRK54_RS06870 are separated by 55 nucleotides LRK54_RS06870 and LRK54_RS06875 are separated by 89 nucleotides LRK54_RS06875 and LRK54_RS06880 are separated by 58 nucleotides LRK54_RS06880 and LRK54_RS06885 are separated by 77 nucleotides
LRK54_RS06865: LRK54_RS06865 - uroporphyrinogen-III C-methyltransferase, at 1,478,839 to 1,479,987
_RS06865
LRK54_RS06870: LRK54_RS06870 - uroporphyrinogen-III synthase, at 1,480,043 to 1,480,813
_RS06870
LRK54_RS06875: LRK54_RS06875 - YiiD C-terminal domain-containing protein, at 1,480,903 to 1,481,376
_RS06875
LRK54_RS06880: LRK54_RS06880 - hypothetical protein, at 1,481,435 to 1,481,848
_RS06880
LRK54_RS06885: LRK54_RS06885 - rhodanese-like domain-containing protein, at 1,481,926 to 1,482,372
_RS06885
Position (kb) 1480
1481
1482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.998 kb on + strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1479.999 kb on - strand at 1480.022 kb on + strand at 1480.022 kb on + strand at 1480.023 kb on - strand at 1480.023 kb on - strand at 1480.023 kb on - strand at 1480.851 kb on + strand at 1480.852 kb on - strand at 1480.852 kb on - strand at 1480.852 kb on - strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.863 kb on + strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1480.864 kb on - strand at 1481.185 kb on + strand, within LRK54_RS06875 at 1481.186 kb on - strand, within LRK54_RS06875 at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.412 kb on + strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.413 kb on - strand at 1481.443 kb on + strand at 1481.444 kb on - strand at 1481.444 kb on - strand at 1481.537 kb on + strand, within LRK54_RS06880 at 1481.537 kb on + strand, within LRK54_RS06880 at 1481.537 kb on + strand, within LRK54_RS06880 at 1481.538 kb on - strand, within LRK54_RS06880 at 1481.538 kb on - strand, within LRK54_RS06880 at 1481.538 kb on - strand, within LRK54_RS06880 at 1481.538 kb on - strand, within LRK54_RS06880 at 1481.663 kb on + strand, within LRK54_RS06880 at 1481.663 kb on + strand, within LRK54_RS06880 at 1481.663 kb on + strand, within LRK54_RS06880 at 1481.663 kb on + strand, within LRK54_RS06880 at 1481.664 kb on - strand, within LRK54_RS06880 at 1481.725 kb on + strand, within LRK54_RS06880 at 1481.726 kb on - strand, within LRK54_RS06880 at 1481.726 kb on - strand, within LRK54_RS06880 at 1481.786 kb on + strand, within LRK54_RS06880 at 1481.786 kb on + strand, within LRK54_RS06880 at 1481.786 kb on + strand, within LRK54_RS06880 at 1481.786 kb on + strand, within LRK54_RS06880 at 1481.787 kb on - strand, within LRK54_RS06880 at 1481.787 kb on - strand, within LRK54_RS06880 at 1481.787 kb on - strand, within LRK54_RS06880 at 1481.787 kb on - strand, within LRK54_RS06880 at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.867 kb on + strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.868 kb on - strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.873 kb on + strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.874 kb on - strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.875 kb on + strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.876 kb on - strand at 1481.887 kb on + strand at 1481.888 kb on - strand at 1482.303 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.304 kb on + strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.305 kb on - strand, within LRK54_RS06885 at 1482.370 kb on + strand at 1482.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with arsenate 15.625 mM remove 1,479,998 + -1.6 1,479,998 + -0.1 1,479,998 + -2.4 1,479,998 + +0.2 1,479,998 + +0.9 1,479,998 + +0.1 1,479,998 + +0.6 1,479,998 + -0.0 1,479,998 + +0.5 1,479,998 + +0.1 1,479,998 + -1.7 1,479,998 + -1.8 1,479,998 + -0.4 1,479,998 + +0.7 1,479,998 + +0.4 1,479,998 + -0.6 1,479,998 + +1.2 1,479,998 + +0.6 1,479,999 - -0.3 1,479,999 - +0.3 1,479,999 - -1.6 1,479,999 - -0.7 1,479,999 - -1.2 1,479,999 - -1.1 1,479,999 - -0.5 1,479,999 - -1.3 1,479,999 - -0.9 1,479,999 - +0.6 1,479,999 - +1.7 1,479,999 - +1.6 1,479,999 - +1.6 1,479,999 - -1.7 1,479,999 - +0.7 1,480,022 + -0.5 1,480,022 + -1.0 1,480,023 - -1.6 1,480,023 - -1.1 1,480,023 - -2.2 1,480,851 + +0.4 1,480,852 - +0.7 1,480,852 - +0.8 1,480,852 - -1.1 1,480,863 + +0.2 1,480,863 + +0.1 1,480,863 + -0.1 1,480,863 + +0.5 1,480,863 + +0.4 1,480,863 + -0.9 1,480,863 + -0.0 1,480,863 + +0.1 1,480,863 + +0.3 1,480,863 + -1.0 1,480,863 + -1.5 1,480,863 + -0.7 1,480,863 + -3.8 1,480,863 + +0.8 1,480,863 + -2.3 1,480,863 + -0.0 1,480,863 + -1.8 1,480,864 - -0.4 1,480,864 - +0.9 1,480,864 - -0.4 1,480,864 - -0.3 1,480,864 - -0.5 1,480,864 - +0.3 1,480,864 - -0.8 1,481,185 + LRK54_RS06875 0.59 -1.1 1,481,186 - LRK54_RS06875 0.60 -2.5 1,481,412 + +2.8 1,481,412 + +1.0 1,481,412 + -2.2 1,481,412 + +0.7 1,481,412 + -0.3 1,481,412 + -0.7 1,481,412 + -0.6 1,481,412 + -0.0 1,481,412 + +0.5 1,481,412 + +0.8 1,481,412 + -0.0 1,481,412 + -1.5 1,481,412 + -2.3 1,481,412 + -0.3 1,481,413 - -0.8 1,481,413 - +0.2 1,481,413 - -2.2 1,481,413 - -0.4 1,481,413 - -1.3 1,481,413 - -1.3 1,481,413 - -0.4 1,481,413 - -1.3 1,481,413 - -0.8 1,481,413 - -0.8 1,481,413 - +1.4 1,481,413 - -3.7 1,481,413 - +1.0 1,481,413 - -0.4 1,481,443 + +1.4 1,481,444 - +1.7 1,481,444 - +0.9 1,481,537 + LRK54_RS06880 0.25 +0.7 1,481,537 + LRK54_RS06880 0.25 +0.4 1,481,537 + LRK54_RS06880 0.25 -0.0 1,481,538 - LRK54_RS06880 0.25 -0.5 1,481,538 - LRK54_RS06880 0.25 +1.7 1,481,538 - LRK54_RS06880 0.25 +0.7 1,481,538 - LRK54_RS06880 0.25 -0.4 1,481,663 + LRK54_RS06880 0.55 -0.1 1,481,663 + LRK54_RS06880 0.55 -0.6 1,481,663 + LRK54_RS06880 0.55 +0.9 1,481,663 + LRK54_RS06880 0.55 +0.6 1,481,664 - LRK54_RS06880 0.55 -0.5 1,481,725 + LRK54_RS06880 0.70 -0.2 1,481,726 - LRK54_RS06880 0.70 +1.6 1,481,726 - LRK54_RS06880 0.70 +0.4 1,481,786 + LRK54_RS06880 0.85 -0.0 1,481,786 + LRK54_RS06880 0.85 +0.1 1,481,786 + LRK54_RS06880 0.85 -1.2 1,481,786 + LRK54_RS06880 0.85 -1.3 1,481,787 - LRK54_RS06880 0.85 -2.8 1,481,787 - LRK54_RS06880 0.85 -0.4 1,481,787 - LRK54_RS06880 0.85 -1.8 1,481,787 - LRK54_RS06880 0.85 +0.7 1,481,867 + -0.2 1,481,867 + -0.4 1,481,867 + -1.7 1,481,867 + +1.0 1,481,867 + +1.3 1,481,867 + -1.4 1,481,867 + +0.3 1,481,867 + -0.6 1,481,867 + +0.5 1,481,867 + -0.7 1,481,867 + +0.0 1,481,867 + +0.7 1,481,867 + -1.4 1,481,867 + -1.1 1,481,867 + +0.1 1,481,867 + +0.7 1,481,867 + +0.4 1,481,867 + -0.5 1,481,867 + -0.5 1,481,867 + -0.0 1,481,867 + +0.5 1,481,867 + +1.1 1,481,867 + +0.5 1,481,867 + -0.7 1,481,867 + +0.5 1,481,867 + -0.1 1,481,867 + -0.4 1,481,867 + +0.5 1,481,868 - -0.3 1,481,868 - -0.7 1,481,868 - -0.5 1,481,868 - +1.7 1,481,868 - -0.5 1,481,868 - -0.9 1,481,868 - +1.7 1,481,868 - -2.1 1,481,868 - +1.7 1,481,868 - +3.9 1,481,868 - +0.1 1,481,868 - +0.0 1,481,868 - -2.0 1,481,868 - -1.0 1,481,868 - -2.9 1,481,868 - +0.1 1,481,868 - -0.5 1,481,868 - -2.1 1,481,868 - -1.6 1,481,868 - -0.2 1,481,873 + -0.2 1,481,873 + +0.7 1,481,873 + -0.1 1,481,873 + -0.5 1,481,873 + -1.2 1,481,873 + +1.0 1,481,873 + -1.2 1,481,873 + -1.9 1,481,873 + -0.4 1,481,873 + +0.8 1,481,873 + -0.6 1,481,873 + +2.0 1,481,873 + -1.6 1,481,874 - -1.9 1,481,874 - -1.0 1,481,874 - +0.1 1,481,874 - -0.3 1,481,874 - -0.9 1,481,874 - -1.0 1,481,874 - -1.1 1,481,874 - +0.1 1,481,874 - -0.6 1,481,874 - -1.6 1,481,874 - -1.4 1,481,874 - -0.4 1,481,874 - -0.0 1,481,874 - -1.0 1,481,874 - +1.3 1,481,874 - -1.1 1,481,874 - -2.4 1,481,874 - -0.4 1,481,874 - -0.6 1,481,874 - -1.2 1,481,874 - -0.4 1,481,874 - -0.3 1,481,874 - -0.1 1,481,874 - -0.2 1,481,874 - -2.2 1,481,874 - -0.3 1,481,874 - +1.4 1,481,874 - -0.7 1,481,874 - +3.3 1,481,874 - -2.0 1,481,874 - -0.6 1,481,875 + +0.2 1,481,875 + -0.3 1,481,875 + +0.5 1,481,875 + -2.1 1,481,875 + -0.3 1,481,875 + -1.1 1,481,875 + +0.3 1,481,875 + +0.5 1,481,875 + +0.2 1,481,875 + -1.7 1,481,876 - -0.5 1,481,876 - -0.3 1,481,876 - -1.5 1,481,876 - -0.0 1,481,876 - -3.0 1,481,876 - -1.5 1,481,876 - -2.3 1,481,887 + -2.7 1,481,888 - -1.4 1,482,303 + LRK54_RS06885 0.84 +0.7 1,482,304 + LRK54_RS06885 0.85 -1.0 1,482,304 + LRK54_RS06885 0.85 -1.4 1,482,304 + LRK54_RS06885 0.85 -2.4 1,482,304 + LRK54_RS06885 0.85 -0.0 1,482,304 + LRK54_RS06885 0.85 -0.9 1,482,304 + LRK54_RS06885 0.85 -0.2 1,482,304 + LRK54_RS06885 0.85 +0.7 1,482,304 + LRK54_RS06885 0.85 +0.5 1,482,304 + LRK54_RS06885 0.85 +0.7 1,482,304 + LRK54_RS06885 0.85 -0.5 1,482,304 + LRK54_RS06885 0.85 +0.3 1,482,304 + LRK54_RS06885 0.85 -1.3 1,482,304 + LRK54_RS06885 0.85 -2.1 1,482,304 + LRK54_RS06885 0.85 -1.3 1,482,304 + LRK54_RS06885 0.85 -3.0 1,482,304 + LRK54_RS06885 0.85 -0.3 1,482,304 + LRK54_RS06885 0.85 +0.6 1,482,304 + LRK54_RS06885 0.85 -0.9 1,482,304 + LRK54_RS06885 0.85 -0.7 1,482,304 + LRK54_RS06885 0.85 -1.1 1,482,304 + LRK54_RS06885 0.85 -2.0 1,482,304 + LRK54_RS06885 0.85 -1.5 1,482,304 + LRK54_RS06885 0.85 -1.0 1,482,304 + LRK54_RS06885 0.85 -2.0 1,482,304 + LRK54_RS06885 0.85 -2.7 1,482,304 + LRK54_RS06885 0.85 -3.5 1,482,304 + LRK54_RS06885 0.85 +0.3 1,482,305 - LRK54_RS06885 0.85 -2.9 1,482,305 - LRK54_RS06885 0.85 +2.3 1,482,305 - LRK54_RS06885 0.85 -1.1 1,482,305 - LRK54_RS06885 0.85 -1.6 1,482,305 - LRK54_RS06885 0.85 -1.1 1,482,305 - LRK54_RS06885 0.85 +0.3 1,482,305 - LRK54_RS06885 0.85 -0.9 1,482,305 - LRK54_RS06885 0.85 -0.9 1,482,305 - LRK54_RS06885 0.85 -1.0 1,482,305 - LRK54_RS06885 0.85 -0.1 1,482,305 - LRK54_RS06885 0.85 -3.1 1,482,305 - LRK54_RS06885 0.85 -1.1 1,482,305 - LRK54_RS06885 0.85 -0.9 1,482,305 - LRK54_RS06885 0.85 -2.4 1,482,305 - LRK54_RS06885 0.85 -1.9 1,482,305 - LRK54_RS06885 0.85 +0.7 1,482,305 - LRK54_RS06885 0.85 +1.3 1,482,370 + -0.2 1,482,370 + +0.9
Or see this region's nucleotide sequence