Experiment: 1648 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.2912.1 and Xcc-8004.2913.1 are separated by 57 nucleotides Xcc-8004.2913.1 and Xcc-8004.2914.1 overlap by 1 nucleotides Xcc-8004.2914.1 and Xcc-8004.2915.1 are separated by 191 nucleotides Xcc-8004.2915.1 and Xcc-8004.2916.1 are separated by 122 nucleotides
Xcc-8004.2912.1: Xcc-8004.2912.1 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 2,835,678 to 2,838,920
Xcc-8004.2912.1
Xcc-8004.2913.1: Xcc-8004.2913.1 - putative; ORF located using Glimmer/Genemark, at 2,838,978 to 2,839,529
Xcc-8004.2913.1
Xcc-8004.2914.1: Xcc-8004.2914.1 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 2,839,529 to 2,840,704
Xcc-8004.2914.1
Xcc-8004.2915.1: Xcc-8004.2915.1 - 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8), at 2,840,896 to 2,841,564
Xcc-8004.2915.1
Xcc-8004.2916.1: Xcc-8004.2916.1 - Uncharacterized protein conserved in bacteria, at 2,841,687 to 2,842,415
Xcc-8004.2916.1
Position (kb)
2839
2840
2841 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2838.931 kb on - strand at 2839.522 kb on - strand at 2840.851 kb on + strand at 2840.851 kb on + strand at 2840.851 kb on + strand at 2840.851 kb on + strand at 2840.852 kb on - strand at 2840.852 kb on - strand at 2840.852 kb on - strand at 2840.880 kb on + strand at 2840.881 kb on - strand at 2840.897 kb on + strand at 2840.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1648 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 2,838,931 - -0.3 2,839,522 - +1.4 2,840,851 + -2.6 2,840,851 + -1.0 2,840,851 + +1.0 2,840,851 + +1.1 2,840,852 - -0.2 2,840,852 - +0.7 2,840,852 - -2.8 2,840,880 + +0.0 2,840,881 - -1.1 2,840,897 + -1.2 2,840,898 - +1.7
Or see this region's nucleotide sequence