Experiment: 1648 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.1048.1 and Xcc-8004.1049.1 are separated by 85 nucleotides Xcc-8004.1049.1 and Xcc-8004.1050.1 overlap by 38 nucleotides Xcc-8004.1050.1 and Xcc-8004.1052.1 are separated by 32 nucleotides
Xcc-8004.1048.1: Xcc-8004.1048.1 - Transcriptional regulator lysR family, at 1,001,091 to 1,001,993
Xcc-8004.1048.1
Xcc-8004.1049.1: Xcc-8004.1049.1 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 1,002,079 to 1,003,557
Xcc-8004.1049.1
Xcc-8004.1050.1: Xcc-8004.1050.1 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 1,003,520 to 1,004,269
Xcc-8004.1050.1
Xcc-8004.1052.1: Xcc-8004.1052.1 - hypothetical protein, at 1,004,302 to 1,005,051
Xcc-8004.1052.1
Position (kb)
1002
1003
1004 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1001.135 kb on + strand at 1001.136 kb on - strand at 1001.136 kb on - strand at 1001.136 kb on - strand at 1001.136 kb on - strand at 1001.323 kb on + strand, within Xcc-8004.1048.1 at 1001.323 kb on + strand, within Xcc-8004.1048.1 at 1001.324 kb on - strand, within Xcc-8004.1048.1 at 1001.324 kb on - strand, within Xcc-8004.1048.1 at 1001.880 kb on + strand, within Xcc-8004.1048.1 at 1002.020 kb on + strand at 1002.057 kb on + strand at 1002.057 kb on + strand at 1002.057 kb on + strand at 1002.057 kb on + strand at 1002.057 kb on + strand at 1002.057 kb on + strand at 1002.058 kb on - strand at 1002.058 kb on - strand at 1002.137 kb on - strand at 1002.196 kb on + strand at 1002.196 kb on + strand at 1002.196 kb on + strand at 1002.197 kb on - strand at 1002.197 kb on - strand at 1002.197 kb on - strand at 1002.197 kb on - strand at 1002.197 kb on - strand at 1002.197 kb on - strand at 1002.531 kb on + strand, within Xcc-8004.1049.1 at 1002.531 kb on + strand, within Xcc-8004.1049.1 at 1002.531 kb on + strand, within Xcc-8004.1049.1 at 1002.531 kb on + strand, within Xcc-8004.1049.1 at 1002.531 kb on + strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.532 kb on - strand, within Xcc-8004.1049.1 at 1002.859 kb on + strand, within Xcc-8004.1049.1 at 1003.008 kb on + strand, within Xcc-8004.1049.1 at 1003.009 kb on - strand, within Xcc-8004.1049.1 at 1003.108 kb on + strand, within Xcc-8004.1049.1 at 1003.109 kb on - strand, within Xcc-8004.1049.1 at 1003.109 kb on - strand, within Xcc-8004.1049.1 at 1003.109 kb on - strand, within Xcc-8004.1049.1 at 1003.109 kb on - strand, within Xcc-8004.1049.1 at 1003.109 kb on - strand, within Xcc-8004.1049.1 at 1003.556 kb on - strand at 1003.599 kb on + strand, within Xcc-8004.1050.1 at 1003.600 kb on - strand, within Xcc-8004.1050.1 at 1003.633 kb on + strand, within Xcc-8004.1050.1 at 1003.634 kb on - strand, within Xcc-8004.1050.1 at 1003.763 kb on + strand, within Xcc-8004.1050.1 at 1003.763 kb on + strand, within Xcc-8004.1050.1 at 1003.970 kb on + strand, within Xcc-8004.1050.1 at 1003.971 kb on - strand, within Xcc-8004.1050.1 at 1004.121 kb on - strand, within Xcc-8004.1050.1 at 1004.274 kb on + strand at 1004.274 kb on + strand at 1004.275 kb on - strand at 1004.347 kb on + strand at 1004.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1648 hydathodes 6 dpi (Dna extract from 8 * 200 hydathodes and pooled together) remove 1,001,135 + +0.2 1,001,136 - +1.4 1,001,136 - +1.8 1,001,136 - +2.3 1,001,136 - +0.9 1,001,323 + Xcc-8004.1048.1 0.26 -0.7 1,001,323 + Xcc-8004.1048.1 0.26 +3.1 1,001,324 - Xcc-8004.1048.1 0.26 -1.1 1,001,324 - Xcc-8004.1048.1 0.26 -1.8 1,001,880 + Xcc-8004.1048.1 0.87 -1.7 1,002,020 + +0.3 1,002,057 + +1.0 1,002,057 + -0.5 1,002,057 + -1.8 1,002,057 + -2.7 1,002,057 + +0.0 1,002,057 + -1.2 1,002,058 - +1.6 1,002,058 - +0.2 1,002,137 - -3.2 1,002,196 + -3.6 1,002,196 + -3.1 1,002,196 + -1.5 1,002,197 - -2.0 1,002,197 - -3.0 1,002,197 - -2.7 1,002,197 - -2.5 1,002,197 - -0.2 1,002,197 - -1.5 1,002,531 + Xcc-8004.1049.1 0.31 -2.8 1,002,531 + Xcc-8004.1049.1 0.31 -3.1 1,002,531 + Xcc-8004.1049.1 0.31 -2.4 1,002,531 + Xcc-8004.1049.1 0.31 +0.6 1,002,531 + Xcc-8004.1049.1 0.31 -1.8 1,002,532 - Xcc-8004.1049.1 0.31 -1.2 1,002,532 - Xcc-8004.1049.1 0.31 -0.4 1,002,532 - Xcc-8004.1049.1 0.31 -2.4 1,002,532 - Xcc-8004.1049.1 0.31 -2.3 1,002,532 - Xcc-8004.1049.1 0.31 -4.3 1,002,532 - Xcc-8004.1049.1 0.31 -2.1 1,002,532 - Xcc-8004.1049.1 0.31 -2.3 1,002,859 + Xcc-8004.1049.1 0.53 -2.5 1,003,008 + Xcc-8004.1049.1 0.63 -3.6 1,003,009 - Xcc-8004.1049.1 0.63 -1.8 1,003,108 + Xcc-8004.1049.1 0.70 -2.5 1,003,109 - Xcc-8004.1049.1 0.70 -2.2 1,003,109 - Xcc-8004.1049.1 0.70 -3.2 1,003,109 - Xcc-8004.1049.1 0.70 -3.5 1,003,109 - Xcc-8004.1049.1 0.70 -2.2 1,003,109 - Xcc-8004.1049.1 0.70 -3.3 1,003,556 - -0.4 1,003,599 + Xcc-8004.1050.1 0.11 -2.5 1,003,600 - Xcc-8004.1050.1 0.11 -3.7 1,003,633 + Xcc-8004.1050.1 0.15 -2.0 1,003,634 - Xcc-8004.1050.1 0.15 -2.9 1,003,763 + Xcc-8004.1050.1 0.32 -1.7 1,003,763 + Xcc-8004.1050.1 0.32 -1.2 1,003,970 + Xcc-8004.1050.1 0.60 -2.2 1,003,971 - Xcc-8004.1050.1 0.60 -2.8 1,004,121 - Xcc-8004.1050.1 0.80 -3.4 1,004,274 + -0.8 1,004,274 + +0.9 1,004,275 - +0.0 1,004,347 + -2.8 1,004,347 + -3.2
Or see this region's nucleotide sequence