Strain Fitness in Rahnella sp. WP5 around EX31_RS10040

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS10035 and EX31_RS10040 are separated by 80 nucleotidesEX31_RS10040 and EX31_RS10045 are separated by 181 nucleotides EX31_RS10035: EX31_RS10035 - cardiolipin synthase, at 213,041 to 214,501 _RS10035 EX31_RS10040: EX31_RS10040 - YciU family protein, at 214,582 to 214,905 _RS10040 EX31_RS10045: EX31_RS10045 - ABC transporter ATP-binding protein, at 215,087 to 216,124 _RS10045 Position (kb) 214 215Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 213.737 kb on + strand, within EX31_RS10035at 213.737 kb on + strand, within EX31_RS10035at 214.000 kb on + strand, within EX31_RS10035at 214.067 kb on + strand, within EX31_RS10035at 214.500 kb on - strandat 214.522 kb on + strandat 214.522 kb on + strandat 214.522 kb on + strandat 214.525 kb on + strandat 214.525 kb on + strandat 214.525 kb on + strandat 214.581 kb on + strandat 214.582 kb on - strandat 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.724 kb on + strand, within EX31_RS10040at 214.726 kb on + strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.727 kb on - strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.827 kb on + strand, within EX31_RS10040at 214.828 kb on - strand, within EX31_RS10040at 214.828 kb on - strand, within EX31_RS10040at 214.836 kb on + strand, within EX31_RS10040at 214.903 kb on + strandat 214.904 kb on - strandat 214.984 kb on + strandat 214.985 kb on - strandat 214.985 kb on - strandat 214.995 kb on + strandat 214.995 kb on + strandat 214.995 kb on + strandat 215.082 kb on - strandat 215.216 kb on - strand, within EX31_RS10045at 215.229 kb on - strand, within EX31_RS10045at 215.314 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.347 kb on - strand, within EX31_RS10045at 215.376 kb on + strand, within EX31_RS10045at 215.376 kb on + strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.377 kb on - strand, within EX31_RS10045at 215.408 kb on + strand, within EX31_RS10045at 215.646 kb on - strand, within EX31_RS10045at 215.699 kb on - strand, within EX31_RS10045at 215.700 kb on + strand, within EX31_RS10045at 215.701 kb on - strand, within EX31_RS10045at 215.822 kb on - strand, within EX31_RS10045

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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213,737 + EX31_RS10035 0.48 -0.7
213,737 + EX31_RS10035 0.48 -1.7
214,000 + EX31_RS10035 0.66 +0.9
214,067 + EX31_RS10035 0.70 +1.4
214,500 - -0.9
214,522 + +2.5
214,522 + -1.6
214,522 + -2.8
214,525 + +0.9
214,525 + -0.4
214,525 + -1.9
214,581 + -0.5
214,582 - +1.4
214,724 + EX31_RS10040 0.44 +1.1
214,724 + EX31_RS10040 0.44 +0.1
214,724 + EX31_RS10040 0.44 -2.5
214,724 + EX31_RS10040 0.44 -0.3
214,724 + EX31_RS10040 0.44 -0.1
214,724 + EX31_RS10040 0.44 -0.5
214,726 + EX31_RS10040 0.44 +2.9
214,727 - EX31_RS10040 0.45 -2.1
214,727 - EX31_RS10040 0.45 -1.5
214,727 - EX31_RS10040 0.45 -0.7
214,827 + EX31_RS10040 0.76 +0.3
214,827 + EX31_RS10040 0.76 -1.7
214,827 + EX31_RS10040 0.76 -0.7
214,827 + EX31_RS10040 0.76 +1.3
214,828 - EX31_RS10040 0.76 -1.7
214,828 - EX31_RS10040 0.76 +2.2
214,836 + EX31_RS10040 0.78 +2.1
214,903 + -0.1
214,904 - -1.1
214,984 + +0.1
214,985 - +0.3
214,985 - -2.1
214,995 + -0.3
214,995 + -0.7
214,995 + -0.7
215,082 - +0.1
215,216 - EX31_RS10045 0.12 -2.9
215,229 - EX31_RS10045 0.14 -2.6
215,314 - EX31_RS10045 0.22 +1.0
215,347 - EX31_RS10045 0.25 -1.1
215,347 - EX31_RS10045 0.25 +0.9
215,347 - EX31_RS10045 0.25 +0.3
215,376 + EX31_RS10045 0.28 -1.5
215,376 + EX31_RS10045 0.28 +0.3
215,377 - EX31_RS10045 0.28 +0.5
215,377 - EX31_RS10045 0.28 +0.5
215,377 - EX31_RS10045 0.28 -0.4
215,408 + EX31_RS10045 0.31 -0.5
215,646 - EX31_RS10045 0.54 +0.4
215,699 - EX31_RS10045 0.59 -0.1
215,700 + EX31_RS10045 0.59 +1.5
215,701 - EX31_RS10045 0.59 -0.0
215,822 - EX31_RS10045 0.71 +0.5

Or see this region's nucleotide sequence