Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22490 and BPHYT_RS22495 are separated by 222 nucleotides BPHYT_RS22495 and BPHYT_RS22500 are separated by 234 nucleotides BPHYT_RS22500 and BPHYT_RS22505 are separated by 183 nucleotides
BPHYT_RS22490: BPHYT_RS22490 - hypothetical protein, at 604,628 to 605,701
_RS22490
BPHYT_RS22495: BPHYT_RS22495 - hypothetical protein, at 605,924 to 606,232
_RS22495
BPHYT_RS22500: BPHYT_RS22500 - transcriptional regulator, at 606,467 to 607,471
_RS22500
BPHYT_RS22505: BPHYT_RS22505 - alpha/beta hydrolase, at 607,655 to 608,518
_RS22505
Position (kb) 606
607
608 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 605.484 kb on + strand, within BPHYT_RS22490 at 605.485 kb on - strand, within BPHYT_RS22490 at 605.603 kb on - strand at 606.392 kb on - strand at 606.629 kb on - strand, within BPHYT_RS22500 at 606.655 kb on - strand, within BPHYT_RS22500 at 606.897 kb on + strand, within BPHYT_RS22500 at 606.898 kb on - strand, within BPHYT_RS22500 at 607.787 kb on - strand, within BPHYT_RS22505 at 607.884 kb on - strand, within BPHYT_RS22505 at 608.255 kb on - strand, within BPHYT_RS22505 at 608.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 605,484 + BPHYT_RS22490 0.80 -0.7 605,485 - BPHYT_RS22490 0.80 +0.2 605,603 - +0.2 606,392 - +0.2 606,629 - BPHYT_RS22500 0.16 +0.4 606,655 - BPHYT_RS22500 0.19 +0.5 606,897 + BPHYT_RS22500 0.43 -1.7 606,898 - BPHYT_RS22500 0.43 -0.7 607,787 - BPHYT_RS22505 0.15 -2.9 607,884 - BPHYT_RS22505 0.27 -4.6 608,255 - BPHYT_RS22505 0.69 -2.5 608,361 - -1.2
Or see this region's nucleotide sequence