Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09525 and BPHYT_RS09530 are separated by 75 nucleotides BPHYT_RS09530 and BPHYT_RS09535 are separated by 11 nucleotides BPHYT_RS09535 and BPHYT_RS09540 are separated by 1 nucleotides BPHYT_RS09540 and BPHYT_RS09545 are separated by 163 nucleotides
BPHYT_RS09525: BPHYT_RS09525 - toxin HipA, at 2,161,711 to 2,163,003
_RS09525
BPHYT_RS09530: BPHYT_RS09530 - peptidyl-prolyl cis-trans isomerase, at 2,163,079 to 2,163,858
_RS09530
BPHYT_RS09535: BPHYT_RS09535 - BolA family transcriptional regulator, at 2,163,870 to 2,164,190
_RS09535
BPHYT_RS09540: BPHYT_RS09540 - intracellular septation protein A, at 2,164,192 to 2,164,722
_RS09540
BPHYT_RS09545: BPHYT_RS09545 - peptide methionine sulfoxide reductase, at 2,164,886 to 2,165,308
_RS09545
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-1
0
1 at 2163.905 kb on - strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.961 kb on + strand, within BPHYT_RS09535 at 2163.962 kb on - strand, within BPHYT_RS09535 at 2164.171 kb on - strand at 2164.171 kb on - strand at 2164.207 kb on - strand at 2164.888 kb on - strand at 2164.904 kb on + strand at 2164.962 kb on - strand, within BPHYT_RS09545 at 2165.145 kb on + strand, within BPHYT_RS09545
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 2,163,905 - BPHYT_RS09535 0.11 -0.7 2,163,961 + BPHYT_RS09535 0.28 -0.2 2,163,961 + BPHYT_RS09535 0.28 -1.6 2,163,962 - BPHYT_RS09535 0.29 -0.5 2,164,171 - -1.7 2,164,171 - -1.0 2,164,207 - +0.3 2,164,888 - +0.4 2,164,904 + +0.3 2,164,962 - BPHYT_RS09545 0.18 +1.0 2,165,145 + BPHYT_RS09545 0.61 -1.2
Or see this region's nucleotide sequence