Strain Fitness in Rahnella sp. WP5 around EX31_RS25140

Experiment: D-Mannitol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS04340 and EX31_RS04345 are separated by 283 nucleotidesEX31_RS04345 and EX31_RS25140 are separated by 16 nucleotidesEX31_RS25140 and EX31_RS04355 are separated by 165 nucleotides EX31_RS04340: EX31_RS04340 - tyrosine-type recombinase/integrase, at 397,627 to 398,832 _RS04340 EX31_RS04345: EX31_RS04345 - hypothetical protein, at 399,116 to 399,430 _RS04345 EX31_RS25140: EX31_RS25140 - AlpA family transcriptional regulator, at 399,447 to 399,653 _RS25140 EX31_RS04355: EX31_RS04355 - hypothetical protein, at 399,819 to 400,628 _RS04355 Position (kb) 399 400Strain fitness (log2 ratio) -2 -1 0 1 2 3at 398.466 kb on + strand, within EX31_RS04340at 398.558 kb on + strand, within EX31_RS04340at 398.631 kb on + strand, within EX31_RS04340at 398.649 kb on + strand, within EX31_RS04340at 398.658 kb on + strand, within EX31_RS04340at 398.684 kb on - strand, within EX31_RS04340at 398.685 kb on + strand, within EX31_RS04340at 398.691 kb on + strand, within EX31_RS04340at 398.803 kb on + strandat 398.833 kb on - strandat 398.852 kb on - strandat 398.858 kb on - strandat 398.861 kb on + strandat 398.862 kb on - strandat 398.874 kb on + strandat 398.974 kb on + strandat 399.070 kb on - strandat 399.566 kb on + strand, within EX31_RS25140at 399.578 kb on - strand, within EX31_RS25140at 399.609 kb on + strand, within EX31_RS25140at 399.699 kb on - strandat 399.704 kb on + strandat 399.705 kb on - strandat 399.709 kb on - strandat 399.726 kb on - strandat 399.726 kb on - strandat 399.802 kb on - strandat 399.802 kb on - strandat 399.802 kb on - strandat 399.813 kb on + strandat 399.813 kb on + strandat 399.814 kb on - strandat 399.891 kb on - strandat 399.909 kb on + strand, within EX31_RS04355at 399.909 kb on + strand, within EX31_RS04355at 399.987 kb on + strand, within EX31_RS04355at 399.999 kb on + strand, within EX31_RS04355at 400.090 kb on - strand, within EX31_RS04355at 400.204 kb on - strand, within EX31_RS04355at 400.271 kb on + strand, within EX31_RS04355at 400.271 kb on + strand, within EX31_RS04355at 400.385 kb on + strand, within EX31_RS04355at 400.385 kb on + strand, within EX31_RS04355at 400.386 kb on - strand, within EX31_RS04355at 400.386 kb on - strand, within EX31_RS04355at 400.503 kb on - strand, within EX31_RS04355at 400.525 kb on - strand, within EX31_RS04355at 400.536 kb on - strand, within EX31_RS04355at 400.536 kb on - strand, within EX31_RS04355at 400.537 kb on + strand, within EX31_RS04355at 400.538 kb on - strand, within EX31_RS04355at 400.540 kb on - strand, within EX31_RS04355at 400.617 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannitol (C)
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398,466 + EX31_RS04340 0.70 -2.1
398,558 + EX31_RS04340 0.77 -0.8
398,631 + EX31_RS04340 0.83 +0.9
398,649 + EX31_RS04340 0.85 +0.1
398,658 + EX31_RS04340 0.85 -0.9
398,684 - EX31_RS04340 0.88 +0.6
398,685 + EX31_RS04340 0.88 +0.1
398,691 + EX31_RS04340 0.88 -1.7
398,803 + -0.4
398,833 - +1.9
398,852 - +1.7
398,858 - -0.4
398,861 + +0.2
398,862 - +0.7
398,874 + +0.5
398,974 + -1.5
399,070 - -0.0
399,566 + EX31_RS25140 0.57 -2.0
399,578 - EX31_RS25140 0.63 -0.6
399,609 + EX31_RS25140 0.78 -1.8
399,699 - -1.9
399,704 + +0.2
399,705 - +0.4
399,709 - -0.2
399,726 - +1.1
399,726 - +0.4
399,802 - -2.5
399,802 - +1.1
399,802 - -1.5
399,813 + +1.1
399,813 + +2.1
399,814 - +0.6
399,891 - -0.5
399,909 + EX31_RS04355 0.11 +0.4
399,909 + EX31_RS04355 0.11 -0.5
399,987 + EX31_RS04355 0.21 +0.1
399,999 + EX31_RS04355 0.22 -0.7
400,090 - EX31_RS04355 0.33 -0.1
400,204 - EX31_RS04355 0.48 -0.6
400,271 + EX31_RS04355 0.56 -0.5
400,271 + EX31_RS04355 0.56 +1.7
400,385 + EX31_RS04355 0.70 +1.1
400,385 + EX31_RS04355 0.70 +3.1
400,386 - EX31_RS04355 0.70 -0.3
400,386 - EX31_RS04355 0.70 -0.9
400,503 - EX31_RS04355 0.84 -1.3
400,525 - EX31_RS04355 0.87 +0.6
400,536 - EX31_RS04355 0.89 +2.1
400,536 - EX31_RS04355 0.89 -0.0
400,537 + EX31_RS04355 0.89 -0.5
400,538 - EX31_RS04355 0.89 +0.3
400,540 - EX31_RS04355 0.89 -0.9
400,617 + -0.9

Or see this region's nucleotide sequence