Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25685 and EX31_RS24665 are separated by 41 nucleotides EX31_RS24665 and EX31_RS24670 are separated by 59 nucleotides EX31_RS24670 and EX31_RS24675 are separated by 146 nucleotides EX31_RS24675 and EX31_RS24680 overlap by 9 nucleotides
EX31_RS25685: EX31_RS25685 - DUF2627 domain-containing protein, at 702,305 to 702,421
_RS25685
EX31_RS24665: EX31_RS24665 - transcription antiterminator/RNA stability regulator CspE, at 702,463 to 702,672
_RS24665
EX31_RS24670: EX31_RS24670 - peptidoglycan editing factor PgeF, at 702,732 to 703,433
_RS24670
EX31_RS24675: EX31_RS24675 - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 703,580 to 704,401
_RS24675
EX31_RS24680: EX31_RS24680 - manganese efflux pump MntP, at 704,393 to 704,965
_RS24680
Position (kb) 702
703
704 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 701.778 kb on + strand at 701.779 kb on - strand at 701.793 kb on - strand at 701.858 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.900 kb on + strand at 701.901 kb on - strand at 701.903 kb on - strand at 701.972 kb on - strand at 701.972 kb on - strand at 701.980 kb on + strand at 701.980 kb on + strand at 702.045 kb on + strand at 702.046 kb on - strand at 702.077 kb on + strand at 702.088 kb on + strand at 702.165 kb on + strand at 702.165 kb on + strand at 702.210 kb on - strand at 702.262 kb on + strand at 702.262 kb on + strand at 702.262 kb on + strand at 702.263 kb on - strand at 702.263 kb on - strand at 702.302 kb on + strand at 702.302 kb on + strand at 702.303 kb on - strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.304 kb on + strand at 702.305 kb on - strand at 702.305 kb on - strand at 702.323 kb on - strand, within EX31_RS25685 at 702.324 kb on + strand, within EX31_RS25685 at 702.324 kb on + strand, within EX31_RS25685 at 702.376 kb on + strand, within EX31_RS25685 at 702.377 kb on - strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.406 kb on + strand, within EX31_RS25685 at 702.407 kb on - strand, within EX31_RS25685 at 702.407 kb on - strand, within EX31_RS25685 at 702.407 kb on - strand, within EX31_RS25685 at 702.407 kb on - strand, within EX31_RS25685 at 702.415 kb on + strand at 702.415 kb on + strand at 702.415 kb on + strand at 702.415 kb on + strand at 702.415 kb on + strand at 702.415 kb on + strand at 702.416 kb on - strand at 702.419 kb on + strand at 702.419 kb on + strand at 702.419 kb on + strand at 702.419 kb on + strand at 702.455 kb on + strand at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.486 kb on + strand, within EX31_RS24665 at 702.554 kb on + strand, within EX31_RS24665 at 702.554 kb on + strand, within EX31_RS24665 at 702.554 kb on + strand, within EX31_RS24665 at 702.554 kb on + strand, within EX31_RS24665 at 702.611 kb on + strand, within EX31_RS24665 at 702.611 kb on + strand, within EX31_RS24665 at 702.659 kb on + strand at 702.659 kb on + strand at 702.659 kb on + strand at 702.659 kb on + strand at 702.670 kb on + strand at 702.670 kb on + strand at 702.670 kb on + strand at 702.680 kb on + strand at 702.680 kb on + strand at 702.680 kb on + strand at 702.680 kb on + strand at 702.687 kb on - strand at 702.762 kb on - strand at 703.027 kb on + strand, within EX31_RS24670 at 703.077 kb on - strand, within EX31_RS24670 at 703.236 kb on - strand, within EX31_RS24670 at 703.308 kb on + strand, within EX31_RS24670 at 703.339 kb on + strand, within EX31_RS24670 at 703.340 kb on - strand, within EX31_RS24670 at 703.340 kb on - strand, within EX31_RS24670 at 703.414 kb on - strand at 703.414 kb on - strand at 703.457 kb on - strand at 703.470 kb on - strand at 703.523 kb on - strand at 703.528 kb on + strand at 703.529 kb on - strand at 703.529 kb on - strand at 703.534 kb on + strand at 703.534 kb on + strand at 703.586 kb on + strand at 703.586 kb on + strand at 703.586 kb on + strand at 703.587 kb on - strand at 703.587 kb on - strand at 703.615 kb on + strand at 703.615 kb on + strand at 703.679 kb on + strand, within EX31_RS24675 at 703.679 kb on + strand, within EX31_RS24675 at 703.699 kb on - strand, within EX31_RS24675 at 703.779 kb on - strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.807 kb on + strand, within EX31_RS24675 at 703.808 kb on - strand, within EX31_RS24675 at 703.871 kb on + strand, within EX31_RS24675 at 703.871 kb on + strand, within EX31_RS24675 at 703.871 kb on + strand, within EX31_RS24675 at 703.874 kb on + strand, within EX31_RS24675 at 703.874 kb on + strand, within EX31_RS24675 at 703.875 kb on - strand, within EX31_RS24675 at 704.050 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.054 kb on + strand, within EX31_RS24675 at 704.055 kb on - strand, within EX31_RS24675 at 704.055 kb on - strand, within EX31_RS24675 at 704.065 kb on + strand, within EX31_RS24675 at 704.139 kb on + strand, within EX31_RS24675 at 704.139 kb on + strand, within EX31_RS24675 at 704.140 kb on - strand, within EX31_RS24675 at 704.140 kb on - strand, within EX31_RS24675 at 704.142 kb on - strand, within EX31_RS24675 at 704.160 kb on + strand, within EX31_RS24675 at 704.160 kb on + strand, within EX31_RS24675 at 704.161 kb on - strand, within EX31_RS24675 at 704.162 kb on + strand, within EX31_RS24675 at 704.162 kb on + strand, within EX31_RS24675 at 704.173 kb on - strand, within EX31_RS24675 at 704.173 kb on - strand, within EX31_RS24675 at 704.240 kb on + strand, within EX31_RS24675 at 704.277 kb on + strand, within EX31_RS24675 at 704.277 kb on + strand, within EX31_RS24675 at 704.332 kb on - strand at 704.379 kb on + strand at 704.379 kb on + strand at 704.399 kb on + strand at 704.403 kb on + strand at 704.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 701,778 + -1.0 701,779 - -1.2 701,793 - -1.0 701,858 + +0.6 701,900 + -0.5 701,900 + -1.2 701,900 + +1.8 701,900 + -1.5 701,901 - +0.8 701,903 - -2.2 701,972 - -1.7 701,972 - +0.1 701,980 + -0.5 701,980 + +0.8 702,045 + -1.6 702,046 - +0.8 702,077 + +1.6 702,088 + -1.5 702,165 + -0.9 702,165 + -0.1 702,210 - +0.6 702,262 + +1.8 702,262 + +4.5 702,262 + +1.6 702,263 - -2.5 702,263 - -1.0 702,302 + -0.0 702,302 + +1.1 702,303 - +0.6 702,304 + +0.4 702,304 + +0.0 702,304 + -3.0 702,304 + -0.3 702,304 + -1.8 702,304 + -1.5 702,304 + -0.2 702,304 + +1.2 702,304 + -0.1 702,304 + +0.5 702,305 - -1.2 702,305 - +1.8 702,323 - EX31_RS25685 0.15 +0.7 702,324 + EX31_RS25685 0.16 +0.8 702,324 + EX31_RS25685 0.16 +1.2 702,376 + EX31_RS25685 0.61 -0.4 702,377 - EX31_RS25685 0.62 +0.3 702,406 + EX31_RS25685 0.86 -1.2 702,406 + EX31_RS25685 0.86 -0.2 702,406 + EX31_RS25685 0.86 +0.7 702,406 + EX31_RS25685 0.86 +0.8 702,406 + EX31_RS25685 0.86 -0.2 702,406 + EX31_RS25685 0.86 +0.3 702,406 + EX31_RS25685 0.86 +1.6 702,406 + EX31_RS25685 0.86 +0.5 702,407 - EX31_RS25685 0.87 +1.0 702,407 - EX31_RS25685 0.87 +1.8 702,407 - EX31_RS25685 0.87 -0.5 702,407 - EX31_RS25685 0.87 -0.3 702,415 + -0.8 702,415 + -0.8 702,415 + +0.7 702,415 + -1.2 702,415 + +0.7 702,415 + -0.5 702,416 - -2.0 702,419 + -0.5 702,419 + +0.7 702,419 + -0.8 702,419 + -0.2 702,455 + -0.0 702,486 + EX31_RS24665 0.11 +0.2 702,486 + EX31_RS24665 0.11 +0.1 702,486 + EX31_RS24665 0.11 -0.2 702,486 + EX31_RS24665 0.11 +0.1 702,486 + EX31_RS24665 0.11 +0.4 702,486 + EX31_RS24665 0.11 -1.6 702,486 + EX31_RS24665 0.11 +0.1 702,554 + EX31_RS24665 0.43 -0.5 702,554 + EX31_RS24665 0.43 -0.7 702,554 + EX31_RS24665 0.43 +0.0 702,554 + EX31_RS24665 0.43 -1.0 702,611 + EX31_RS24665 0.70 -0.5 702,611 + EX31_RS24665 0.70 +1.4 702,659 + +0.3 702,659 + -2.1 702,659 + -0.5 702,659 + -0.5 702,670 + +0.8 702,670 + +0.3 702,670 + +0.8 702,680 + -0.7 702,680 + +0.0 702,680 + -0.1 702,680 + +0.6 702,687 - +0.8 702,762 - +0.5 703,027 + EX31_RS24670 0.42 -1.2 703,077 - EX31_RS24670 0.49 +0.8 703,236 - EX31_RS24670 0.72 -3.4 703,308 + EX31_RS24670 0.82 -1.9 703,339 + EX31_RS24670 0.86 -0.7 703,340 - EX31_RS24670 0.87 +0.3 703,340 - EX31_RS24670 0.87 +0.5 703,414 - +0.6 703,414 - -1.2 703,457 - +0.3 703,470 - +0.8 703,523 - -0.7 703,528 + -0.2 703,529 - +0.8 703,529 - +0.4 703,534 + +1.4 703,534 + -1.2 703,586 + -1.3 703,586 + -0.7 703,586 + +0.8 703,587 - +1.3 703,587 - +2.4 703,615 + -1.7 703,615 + +2.4 703,679 + EX31_RS24675 0.12 -1.2 703,679 + EX31_RS24675 0.12 +1.2 703,699 - EX31_RS24675 0.14 +1.1 703,779 - EX31_RS24675 0.24 -0.4 703,807 + EX31_RS24675 0.28 -0.6 703,807 + EX31_RS24675 0.28 -0.7 703,807 + EX31_RS24675 0.28 -0.7 703,807 + EX31_RS24675 0.28 +1.1 703,807 + EX31_RS24675 0.28 -2.3 703,807 + EX31_RS24675 0.28 -0.5 703,808 - EX31_RS24675 0.28 -0.2 703,871 + EX31_RS24675 0.35 +0.6 703,871 + EX31_RS24675 0.35 +0.8 703,871 + EX31_RS24675 0.35 -1.1 703,874 + EX31_RS24675 0.36 +0.6 703,874 + EX31_RS24675 0.36 -0.7 703,875 - EX31_RS24675 0.36 +1.8 704,050 + EX31_RS24675 0.57 +0.8 704,054 + EX31_RS24675 0.58 -0.6 704,054 + EX31_RS24675 0.58 +0.2 704,054 + EX31_RS24675 0.58 -1.0 704,054 + EX31_RS24675 0.58 +1.6 704,054 + EX31_RS24675 0.58 -1.5 704,054 + EX31_RS24675 0.58 +0.8 704,054 + EX31_RS24675 0.58 -1.0 704,054 + EX31_RS24675 0.58 +0.3 704,055 - EX31_RS24675 0.58 +0.1 704,055 - EX31_RS24675 0.58 -1.0 704,065 + EX31_RS24675 0.59 +1.1 704,139 + EX31_RS24675 0.68 +1.8 704,139 + EX31_RS24675 0.68 -0.2 704,140 - EX31_RS24675 0.68 -0.7 704,140 - EX31_RS24675 0.68 -1.0 704,142 - EX31_RS24675 0.68 +0.8 704,160 + EX31_RS24675 0.71 -0.7 704,160 + EX31_RS24675 0.71 -0.5 704,161 - EX31_RS24675 0.71 -0.7 704,162 + EX31_RS24675 0.71 +0.6 704,162 + EX31_RS24675 0.71 -1.7 704,173 - EX31_RS24675 0.72 +0.8 704,173 - EX31_RS24675 0.72 -0.7 704,240 + EX31_RS24675 0.80 +0.6 704,277 + EX31_RS24675 0.85 +0.8 704,277 + EX31_RS24675 0.85 +1.8 704,332 - +0.3 704,379 + -2.0 704,379 + +0.3 704,399 + -1.0 704,403 + +0.0 704,404 - -0.7
Or see this region's nucleotide sequence