Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22615 and EX31_RS22620 are separated by 155 nucleotides EX31_RS22620 and EX31_RS22625 overlap by 4 nucleotides EX31_RS22625 and EX31_RS22630 are separated by 180 nucleotides
EX31_RS22615: EX31_RS22615 - ABC transporter permease, at 277,611 to 280,043
_RS22615
EX31_RS22620: EX31_RS22620 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 280,199 to 281,263
_RS22620
EX31_RS22625: EX31_RS22625 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 281,260 to 281,769
_RS22625
EX31_RS22630: EX31_RS22630 - tRNA-(ms[2]io[6]A)-hydroxylase, at 281,950 to 282,582
_RS22630
Position (kb)
280
281
282 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 279.200 kb on + strand, within EX31_RS22615 at 279.222 kb on - strand, within EX31_RS22615 at 279.352 kb on + strand, within EX31_RS22615 at 279.581 kb on + strand, within EX31_RS22615 at 279.646 kb on + strand, within EX31_RS22615 at 279.647 kb on - strand, within EX31_RS22615 at 279.647 kb on - strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.649 kb on + strand, within EX31_RS22615 at 279.650 kb on - strand, within EX31_RS22615 at 279.650 kb on - strand, within EX31_RS22615 at 279.650 kb on - strand, within EX31_RS22615 at 279.650 kb on - strand, within EX31_RS22615 at 279.650 kb on - strand, within EX31_RS22615 at 279.773 kb on + strand, within EX31_RS22615 at 279.773 kb on + strand, within EX31_RS22615 at 279.773 kb on + strand, within EX31_RS22615 at 279.773 kb on + strand, within EX31_RS22615 at 279.774 kb on - strand, within EX31_RS22615 at 279.927 kb on + strand at 279.927 kb on + strand at 280.032 kb on + strand at 280.051 kb on - strand at 280.098 kb on + strand at 280.143 kb on + strand at 280.144 kb on - strand at 280.153 kb on + strand at 280.154 kb on - strand at 280.154 kb on - strand at 280.154 kb on - strand at 280.157 kb on + strand at 280.157 kb on + strand at 280.158 kb on - strand at 280.180 kb on + strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.181 kb on - strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.184 kb on - strand at 280.236 kb on + strand at 280.237 kb on - strand at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.497 kb on + strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.498 kb on - strand, within EX31_RS22620 at 280.608 kb on + strand, within EX31_RS22620 at 280.608 kb on + strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.609 kb on - strand, within EX31_RS22620 at 280.735 kb on - strand, within EX31_RS22620 at 280.747 kb on + strand, within EX31_RS22620 at 280.748 kb on - strand, within EX31_RS22620 at 280.824 kb on + strand, within EX31_RS22620 at 280.825 kb on - strand, within EX31_RS22620 at 280.843 kb on + strand, within EX31_RS22620 at 280.843 kb on + strand, within EX31_RS22620 at 280.844 kb on - strand, within EX31_RS22620 at 280.938 kb on - strand, within EX31_RS22620 at 281.182 kb on - strand at 281.404 kb on - strand, within EX31_RS22625 at 281.404 kb on - strand, within EX31_RS22625 at 281.456 kb on + strand, within EX31_RS22625 at 281.614 kb on - strand, within EX31_RS22625 at 281.667 kb on + strand, within EX31_RS22625 at 281.668 kb on - strand, within EX31_RS22625 at 281.668 kb on - strand, within EX31_RS22625 at 281.775 kb on + strand at 281.775 kb on + strand at 281.775 kb on + strand at 281.775 kb on + strand at 281.776 kb on - strand at 281.798 kb on - strand at 281.821 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.916 kb on + strand at 281.917 kb on - strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.918 kb on + strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.919 kb on - strand at 281.949 kb on + strand at 281.963 kb on + strand at 282.060 kb on + strand, within EX31_RS22630 at 282.109 kb on + strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.110 kb on - strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.207 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.210 kb on + strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.211 kb on - strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.214 kb on + strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.215 kb on - strand, within EX31_RS22630 at 282.216 kb on + strand, within EX31_RS22630 at 282.261 kb on + strand, within EX31_RS22630 at 282.261 kb on + strand, within EX31_RS22630
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 279,200 + EX31_RS22615 0.65 +0.9 279,222 - EX31_RS22615 0.66 +0.3 279,352 + EX31_RS22615 0.72 +0.1 279,581 + EX31_RS22615 0.81 +0.1 279,646 + EX31_RS22615 0.84 -0.1 279,647 - EX31_RS22615 0.84 +1.3 279,647 - EX31_RS22615 0.84 -1.7 279,649 + EX31_RS22615 0.84 +0.6 279,649 + EX31_RS22615 0.84 -0.1 279,649 + EX31_RS22615 0.84 -0.1 279,649 + EX31_RS22615 0.84 -1.4 279,649 + EX31_RS22615 0.84 +1.1 279,649 + EX31_RS22615 0.84 -0.7 279,649 + EX31_RS22615 0.84 -0.1 279,649 + EX31_RS22615 0.84 -1.2 279,649 + EX31_RS22615 0.84 +3.6 279,650 - EX31_RS22615 0.84 +2.5 279,650 - EX31_RS22615 0.84 +0.7 279,650 - EX31_RS22615 0.84 +1.5 279,650 - EX31_RS22615 0.84 -0.7 279,650 - EX31_RS22615 0.84 -1.0 279,773 + EX31_RS22615 0.89 -0.7 279,773 + EX31_RS22615 0.89 +0.2 279,773 + EX31_RS22615 0.89 -2.7 279,773 + EX31_RS22615 0.89 +0.2 279,774 - EX31_RS22615 0.89 -0.1 279,927 + -0.5 279,927 + -0.5 280,032 + -0.2 280,051 - -0.9 280,098 + -0.4 280,143 + +0.6 280,144 - +1.0 280,153 + -0.8 280,154 - +1.9 280,154 - -1.2 280,154 - -0.7 280,157 + +0.6 280,157 + -0.7 280,158 - +0.5 280,180 + +0.1 280,181 - +1.2 280,181 - +1.6 280,181 - -0.3 280,181 - +0.6 280,183 + -0.2 280,183 + -1.4 280,184 - +1.6 280,236 + -2.1 280,237 - -0.1 280,497 + EX31_RS22620 0.28 -1.9 280,497 + EX31_RS22620 0.28 -1.4 280,497 + EX31_RS22620 0.28 -3.2 280,497 + EX31_RS22620 0.28 -1.4 280,497 + EX31_RS22620 0.28 -1.1 280,498 - EX31_RS22620 0.28 -1.4 280,498 - EX31_RS22620 0.28 -3.7 280,498 - EX31_RS22620 0.28 -1.1 280,498 - EX31_RS22620 0.28 -2.7 280,608 + EX31_RS22620 0.38 -3.4 280,608 + EX31_RS22620 0.38 -3.8 280,609 - EX31_RS22620 0.38 -0.1 280,609 - EX31_RS22620 0.38 -2.9 280,609 - EX31_RS22620 0.38 -1.4 280,609 - EX31_RS22620 0.38 -2.4 280,609 - EX31_RS22620 0.38 -1.1 280,735 - EX31_RS22620 0.50 -2.8 280,747 + EX31_RS22620 0.51 -2.7 280,748 - EX31_RS22620 0.52 -2.6 280,824 + EX31_RS22620 0.59 -1.4 280,825 - EX31_RS22620 0.59 -2.7 280,843 + EX31_RS22620 0.60 -2.3 280,843 + EX31_RS22620 0.60 -1.1 280,844 - EX31_RS22620 0.61 -1.7 280,938 - EX31_RS22620 0.69 -2.4 281,182 - -2.9 281,404 - EX31_RS22625 0.28 -1.4 281,404 - EX31_RS22625 0.28 -1.9 281,456 + EX31_RS22625 0.38 -0.7 281,614 - EX31_RS22625 0.69 -1.7 281,667 + EX31_RS22625 0.80 -1.4 281,668 - EX31_RS22625 0.80 -2.1 281,668 - EX31_RS22625 0.80 -2.1 281,775 + -3.0 281,775 + -2.7 281,775 + -4.0 281,775 + -3.1 281,776 - -1.9 281,798 - -3.4 281,821 + -4.0 281,916 + -0.1 281,916 + +0.4 281,916 + +0.5 281,916 + +0.3 281,917 - -1.0 281,918 + +0.2 281,918 + -1.9 281,918 + +0.5 281,918 + -1.1 281,918 + -1.1 281,918 + -0.5 281,918 + +0.4 281,918 + -0.0 281,918 + +0.2 281,918 + -0.6 281,918 + +2.2 281,918 + -0.8 281,919 - +1.3 281,919 - -0.3 281,919 - +0.4 281,919 - +1.3 281,919 - +1.6 281,919 - +1.0 281,919 - +0.5 281,919 - +1.3 281,919 - -0.1 281,949 + -0.6 281,963 + -0.3 282,060 + EX31_RS22630 0.17 -0.3 282,109 + EX31_RS22630 0.25 +1.0 282,110 - EX31_RS22630 0.25 +0.9 282,110 - EX31_RS22630 0.25 -0.1 282,110 - EX31_RS22630 0.25 -1.4 282,207 + EX31_RS22630 0.41 +0.9 282,207 + EX31_RS22630 0.41 +1.9 282,207 + EX31_RS22630 0.41 +1.0 282,210 + EX31_RS22630 0.41 -0.7 282,210 + EX31_RS22630 0.41 +0.9 282,210 + EX31_RS22630 0.41 +1.6 282,210 + EX31_RS22630 0.41 +2.9 282,210 + EX31_RS22630 0.41 +1.2 282,210 + EX31_RS22630 0.41 +2.3 282,210 + EX31_RS22630 0.41 +0.8 282,210 + EX31_RS22630 0.41 +0.9 282,210 + EX31_RS22630 0.41 +0.6 282,210 + EX31_RS22630 0.41 +0.9 282,210 + EX31_RS22630 0.41 -2.6 282,210 + EX31_RS22630 0.41 -0.1 282,211 - EX31_RS22630 0.41 -0.0 282,211 - EX31_RS22630 0.41 +0.3 282,211 - EX31_RS22630 0.41 -0.1 282,211 - EX31_RS22630 0.41 +1.9 282,214 + EX31_RS22630 0.42 -0.4 282,214 + EX31_RS22630 0.42 -0.6 282,214 + EX31_RS22630 0.42 -1.8 282,214 + EX31_RS22630 0.42 +1.5 282,214 + EX31_RS22630 0.42 +1.2 282,215 - EX31_RS22630 0.42 +2.1 282,215 - EX31_RS22630 0.42 -0.7 282,215 - EX31_RS22630 0.42 +0.4 282,215 - EX31_RS22630 0.42 -1.1 282,216 + EX31_RS22630 0.42 +1.7 282,261 + EX31_RS22630 0.49 -0.1 282,261 + EX31_RS22630 0.49 -0.1
Or see this region's nucleotide sequence