Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20195 and EX31_RS20200 overlap by 20 nucleotides EX31_RS20200 and EX31_RS20205 are separated by 102 nucleotides EX31_RS20205 and EX31_RS20210 are separated by 115 nucleotides
EX31_RS20195: EX31_RS20195 - HAMP domain-containing protein, at 667,456 to 668,805
_RS20195
EX31_RS20200: EX31_RS20200 - response regulator transcription factor, at 668,786 to 669,469
_RS20200
EX31_RS20205: EX31_RS20205 - cysteine synthase CysM, at 669,572 to 670,456
_RS20205
EX31_RS20210: EX31_RS20210 - sulfate/thiosulfate ABC transporter ATP-binding protein CysA, at 670,572 to 671,666
_RS20210
Position (kb) 669
670
671 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 668.631 kb on + strand, within EX31_RS20195 at 668.632 kb on - strand, within EX31_RS20195 at 668.690 kb on + strand at 668.690 kb on + strand at 668.690 kb on + strand at 668.691 kb on - strand at 668.691 kb on - strand at 668.737 kb on - strand at 668.755 kb on + strand at 668.756 kb on - strand at 668.879 kb on - strand, within EX31_RS20200 at 668.879 kb on - strand, within EX31_RS20200 at 668.891 kb on - strand, within EX31_RS20200 at 668.901 kb on + strand, within EX31_RS20200 at 668.901 kb on + strand, within EX31_RS20200 at 668.902 kb on - strand, within EX31_RS20200 at 668.973 kb on - strand, within EX31_RS20200 at 668.973 kb on - strand, within EX31_RS20200 at 668.973 kb on - strand, within EX31_RS20200 at 668.978 kb on + strand, within EX31_RS20200 at 668.979 kb on - strand, within EX31_RS20200 at 668.979 kb on - strand, within EX31_RS20200 at 668.979 kb on - strand, within EX31_RS20200 at 668.979 kb on - strand, within EX31_RS20200 at 669.072 kb on + strand, within EX31_RS20200 at 669.073 kb on - strand, within EX31_RS20200 at 669.073 kb on - strand, within EX31_RS20200 at 669.073 kb on - strand, within EX31_RS20200 at 669.073 kb on - strand, within EX31_RS20200 at 669.073 kb on - strand, within EX31_RS20200 at 669.180 kb on + strand, within EX31_RS20200 at 669.180 kb on + strand, within EX31_RS20200 at 669.180 kb on + strand, within EX31_RS20200 at 669.180 kb on + strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.181 kb on - strand, within EX31_RS20200 at 669.284 kb on + strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.285 kb on - strand, within EX31_RS20200 at 669.313 kb on + strand, within EX31_RS20200 at 669.313 kb on + strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.314 kb on - strand, within EX31_RS20200 at 669.334 kb on + strand, within EX31_RS20200 at 669.334 kb on + strand, within EX31_RS20200 at 669.336 kb on + strand, within EX31_RS20200 at 669.415 kb on - strand at 669.415 kb on - strand at 669.415 kb on - strand at 669.415 kb on - strand at 669.415 kb on - strand at 669.434 kb on + strand at 669.435 kb on - strand at 669.435 kb on - strand at 669.512 kb on + strand at 669.513 kb on - strand at 669.513 kb on - strand at 669.513 kb on - strand at 669.524 kb on + strand at 669.524 kb on + strand at 669.524 kb on + strand at 669.525 kb on - strand at 669.525 kb on - strand at 669.525 kb on - strand at 669.525 kb on - strand at 669.538 kb on - strand at 669.538 kb on - strand at 669.565 kb on + strand at 669.566 kb on - strand at 669.566 kb on - strand at 669.567 kb on + strand at 669.567 kb on + strand at 669.567 kb on + strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.568 kb on - strand at 669.600 kb on + strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.601 kb on - strand at 669.622 kb on + strand at 669.623 kb on - strand at 669.623 kb on - strand at 669.666 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.710 kb on - strand, within EX31_RS20205 at 669.810 kb on + strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 669.811 kb on - strand, within EX31_RS20205 at 670.074 kb on + strand, within EX31_RS20205 at 670.141 kb on - strand, within EX31_RS20205 at 670.141 kb on - strand, within EX31_RS20205 at 670.174 kb on + strand, within EX31_RS20205 at 670.175 kb on - strand, within EX31_RS20205 at 670.196 kb on - strand, within EX31_RS20205 at 670.196 kb on - strand, within EX31_RS20205 at 670.316 kb on - strand, within EX31_RS20205 at 670.355 kb on - strand, within EX31_RS20205 at 670.355 kb on - strand, within EX31_RS20205 at 670.409 kb on + strand at 670.409 kb on + strand at 670.409 kb on + strand at 670.409 kb on + strand at 670.410 kb on - strand at 670.410 kb on - strand at 670.410 kb on - strand at 670.427 kb on - strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.437 kb on + strand at 670.438 kb on - strand at 670.451 kb on - strand at 670.480 kb on + strand at 670.481 kb on - strand at 670.483 kb on - strand at 670.503 kb on + strand at 670.504 kb on - strand at 670.602 kb on + strand at 670.621 kb on + strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.622 kb on - strand at 670.648 kb on + strand at 670.649 kb on - strand at 670.649 kb on - strand at 670.677 kb on - strand at 670.743 kb on - strand, within EX31_RS20210 at 670.743 kb on - strand, within EX31_RS20210 at 670.897 kb on + strand, within EX31_RS20210 at 670.898 kb on - strand, within EX31_RS20210 at 670.898 kb on - strand, within EX31_RS20210 at 670.910 kb on - strand, within EX31_RS20210 at 670.944 kb on - strand, within EX31_RS20210 at 670.947 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.028 kb on - strand, within EX31_RS20210 at 671.150 kb on + strand, within EX31_RS20210 at 671.403 kb on - strand, within EX31_RS20210 at 671.419 kb on + strand, within EX31_RS20210 at 671.420 kb on - strand, within EX31_RS20210 at 671.420 kb on - strand, within EX31_RS20210
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 668,631 + EX31_RS20195 0.87 -2.2 668,632 - EX31_RS20195 0.87 +0.7 668,690 + +0.9 668,690 + -0.8 668,690 + -1.6 668,691 - +1.7 668,691 - -1.4 668,737 - -0.3 668,755 + -0.1 668,756 - +1.5 668,879 - EX31_RS20200 0.14 +0.6 668,879 - EX31_RS20200 0.14 -1.2 668,891 - EX31_RS20200 0.15 +0.4 668,901 + EX31_RS20200 0.17 -0.7 668,901 + EX31_RS20200 0.17 -2.1 668,902 - EX31_RS20200 0.17 +0.6 668,973 - EX31_RS20200 0.27 -2.1 668,973 - EX31_RS20200 0.27 -1.0 668,973 - EX31_RS20200 0.27 +0.5 668,978 + EX31_RS20200 0.28 +0.4 668,979 - EX31_RS20200 0.28 -0.1 668,979 - EX31_RS20200 0.28 +0.4 668,979 - EX31_RS20200 0.28 -0.3 668,979 - EX31_RS20200 0.28 +1.1 669,072 + EX31_RS20200 0.42 -0.6 669,073 - EX31_RS20200 0.42 +0.7 669,073 - EX31_RS20200 0.42 +0.3 669,073 - EX31_RS20200 0.42 +0.1 669,073 - EX31_RS20200 0.42 +0.9 669,073 - EX31_RS20200 0.42 -0.9 669,180 + EX31_RS20200 0.58 -0.7 669,180 + EX31_RS20200 0.58 +1.3 669,180 + EX31_RS20200 0.58 +0.7 669,180 + EX31_RS20200 0.58 +0.2 669,181 - EX31_RS20200 0.58 +0.1 669,181 - EX31_RS20200 0.58 +0.5 669,181 - EX31_RS20200 0.58 -1.3 669,181 - EX31_RS20200 0.58 -0.3 669,181 - EX31_RS20200 0.58 -0.8 669,181 - EX31_RS20200 0.58 -0.1 669,181 - EX31_RS20200 0.58 -0.6 669,181 - EX31_RS20200 0.58 +1.1 669,181 - EX31_RS20200 0.58 +0.5 669,181 - EX31_RS20200 0.58 -0.6 669,181 - EX31_RS20200 0.58 +1.4 669,284 + EX31_RS20200 0.73 +3.1 669,285 - EX31_RS20200 0.73 -0.6 669,285 - EX31_RS20200 0.73 -0.5 669,285 - EX31_RS20200 0.73 -1.9 669,285 - EX31_RS20200 0.73 -0.6 669,285 - EX31_RS20200 0.73 +0.7 669,285 - EX31_RS20200 0.73 +0.6 669,285 - EX31_RS20200 0.73 +1.9 669,285 - EX31_RS20200 0.73 -0.5 669,285 - EX31_RS20200 0.73 +0.9 669,285 - EX31_RS20200 0.73 +0.7 669,285 - EX31_RS20200 0.73 -0.5 669,313 + EX31_RS20200 0.77 -0.6 669,313 + EX31_RS20200 0.77 +1.9 669,314 - EX31_RS20200 0.77 +0.4 669,314 - EX31_RS20200 0.77 +0.9 669,314 - EX31_RS20200 0.77 -0.3 669,314 - EX31_RS20200 0.77 +0.5 669,314 - EX31_RS20200 0.77 +0.3 669,314 - EX31_RS20200 0.77 -0.7 669,334 + EX31_RS20200 0.80 -2.1 669,334 + EX31_RS20200 0.80 +0.1 669,336 + EX31_RS20200 0.80 -0.1 669,415 - -0.6 669,415 - +0.8 669,415 - -0.2 669,415 - +0.2 669,415 - -1.1 669,434 + -0.1 669,435 - -2.6 669,435 - +0.9 669,512 + +0.6 669,513 - -0.8 669,513 - +0.1 669,513 - -0.2 669,524 + +0.7 669,524 + +0.5 669,524 + -0.4 669,525 - +0.0 669,525 - +1.2 669,525 - +0.7 669,525 - -0.2 669,538 - +0.8 669,538 - -0.5 669,565 + +0.9 669,566 - +0.4 669,566 - +0.9 669,567 + +1.5 669,567 + -0.1 669,567 + -2.4 669,568 - +1.1 669,568 - -0.7 669,568 - +0.6 669,568 - -0.1 669,568 - -0.6 669,568 - +0.8 669,568 - +0.1 669,600 + -1.6 669,601 - -0.1 669,601 - -2.3 669,601 - -1.4 669,601 - -1.5 669,622 + +0.5 669,623 - -2.4 669,623 - -1.2 669,666 - EX31_RS20205 0.11 -0.6 669,710 - EX31_RS20205 0.16 -0.6 669,710 - EX31_RS20205 0.16 -0.4 669,710 - EX31_RS20205 0.16 -1.4 669,710 - EX31_RS20205 0.16 -0.9 669,710 - EX31_RS20205 0.16 -0.6 669,710 - EX31_RS20205 0.16 -0.1 669,710 - EX31_RS20205 0.16 -2.4 669,810 + EX31_RS20205 0.27 -0.8 669,811 - EX31_RS20205 0.27 -1.5 669,811 - EX31_RS20205 0.27 -0.8 669,811 - EX31_RS20205 0.27 -0.3 670,074 + EX31_RS20205 0.57 -2.2 670,141 - EX31_RS20205 0.64 -0.1 670,141 - EX31_RS20205 0.64 -0.9 670,174 + EX31_RS20205 0.68 -2.4 670,175 - EX31_RS20205 0.68 +0.1 670,196 - EX31_RS20205 0.71 -0.3 670,196 - EX31_RS20205 0.71 +0.9 670,316 - EX31_RS20205 0.84 -1.6 670,355 - EX31_RS20205 0.88 -1.6 670,355 - EX31_RS20205 0.88 -0.4 670,409 + -3.6 670,409 + -0.1 670,409 + -3.6 670,409 + -0.6 670,410 - -2.9 670,410 - -0.1 670,410 - -0.7 670,427 - -1.3 670,437 + -0.3 670,437 + +0.9 670,437 + -0.1 670,437 + -0.9 670,438 - -0.3 670,451 - -1.9 670,480 + +0.4 670,481 - +0.2 670,483 - +0.1 670,503 + -0.1 670,504 - +0.5 670,602 + +0.2 670,621 + +0.1 670,622 - +0.0 670,622 - -1.1 670,622 - +1.2 670,622 - -0.2 670,622 - +0.3 670,622 - -0.5 670,622 - -0.6 670,648 + -0.6 670,649 - +0.3 670,649 - +0.8 670,677 - -0.4 670,743 - EX31_RS20210 0.16 -3.1 670,743 - EX31_RS20210 0.16 -0.1 670,897 + EX31_RS20210 0.30 +0.1 670,898 - EX31_RS20210 0.30 -0.6 670,898 - EX31_RS20210 0.30 -1.2 670,910 - EX31_RS20210 0.31 +1.8 670,944 - EX31_RS20210 0.34 +1.3 670,947 - EX31_RS20210 0.34 -0.5 671,028 - EX31_RS20210 0.42 -0.8 671,028 - EX31_RS20210 0.42 +1.3 671,028 - EX31_RS20210 0.42 +1.2 671,150 + EX31_RS20210 0.53 -0.1 671,403 - EX31_RS20210 0.76 +0.4 671,419 + EX31_RS20210 0.77 -0.6 671,420 - EX31_RS20210 0.77 -1.5 671,420 - EX31_RS20210 0.77 -1.4
Or see this region's nucleotide sequence