Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20175 and EX31_RS20180 are separated by 403 nucleotides EX31_RS20180 and EX31_RS20185 are separated by 50 nucleotides
EX31_RS20175: EX31_RS20175 - cysteine synthase A, at 663,437 to 664,402
_RS20175
EX31_RS20180: EX31_RS20180 - phosphocarrier protein Hpr, at 664,806 to 665,063
_RS20180
EX31_RS20185: EX31_RS20185 - phosphoenolpyruvate-protein phosphotransferase PtsI, at 665,114 to 666,841
_RS20185
Position (kb) 664
665
666 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 663.848 kb on + strand, within EX31_RS20175 at 663.848 kb on + strand, within EX31_RS20175 at 663.853 kb on + strand, within EX31_RS20175 at 663.853 kb on + strand, within EX31_RS20175 at 663.854 kb on - strand, within EX31_RS20175 at 663.854 kb on - strand, within EX31_RS20175 at 663.928 kb on + strand, within EX31_RS20175 at 663.928 kb on + strand, within EX31_RS20175 at 663.980 kb on - strand, within EX31_RS20175 at 663.980 kb on - strand, within EX31_RS20175 at 664.106 kb on - strand, within EX31_RS20175 at 664.132 kb on + strand, within EX31_RS20175 at 664.176 kb on + strand, within EX31_RS20175 at 664.374 kb on + strand at 664.375 kb on - strand at 664.400 kb on + strand at 664.400 kb on + strand at 664.400 kb on + strand at 664.414 kb on + strand at 664.415 kb on - strand at 664.471 kb on + strand at 664.471 kb on + strand at 664.471 kb on + strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.506 kb on + strand at 664.507 kb on - strand at 664.507 kb on - strand at 664.510 kb on - strand at 664.510 kb on - strand at 664.513 kb on + strand at 664.514 kb on - strand at 664.612 kb on + strand at 664.612 kb on + strand at 664.613 kb on - strand at 664.623 kb on + strand at 664.624 kb on - strand at 664.624 kb on - strand at 664.734 kb on - strand at 664.740 kb on + strand at 664.741 kb on - strand at 664.754 kb on + strand at 664.755 kb on - strand at 664.759 kb on + strand at 664.766 kb on + strand at 664.766 kb on + strand at 664.766 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.786 kb on - strand at 664.788 kb on + strand at 664.788 kb on + strand at 664.788 kb on + strand at 664.789 kb on - strand at 664.789 kb on - strand at 664.789 kb on - strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.829 kb on + strand at 664.838 kb on + strand, within EX31_RS20180 at 664.838 kb on + strand, within EX31_RS20180 at 664.937 kb on + strand, within EX31_RS20180 at 664.937 kb on + strand, within EX31_RS20180 at 665.038 kb on + strand at 665.061 kb on + strand at 665.061 kb on + strand at 665.065 kb on - strand at 665.065 kb on - strand at 665.078 kb on + strand at 665.078 kb on + strand at 665.078 kb on + strand at 665.081 kb on - strand at 665.107 kb on + strand at 665.107 kb on + strand at 665.107 kb on + strand at 665.113 kb on + strand at 665.382 kb on + strand, within EX31_RS20185 at 665.581 kb on + strand, within EX31_RS20185 at 665.650 kb on + strand, within EX31_RS20185 at 665.671 kb on + strand, within EX31_RS20185 at 665.672 kb on - strand, within EX31_RS20185 at 665.762 kb on + strand, within EX31_RS20185 at 665.762 kb on + strand, within EX31_RS20185 at 665.762 kb on + strand, within EX31_RS20185 at 665.763 kb on - strand, within EX31_RS20185 at 665.782 kb on + strand, within EX31_RS20185 at 665.795 kb on + strand, within EX31_RS20185 at 665.849 kb on + strand, within EX31_RS20185 at 665.849 kb on + strand, within EX31_RS20185 at 666.062 kb on + strand, within EX31_RS20185 at 666.062 kb on + strand, within EX31_RS20185
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 663,848 + EX31_RS20175 0.43 -4.0 663,848 + EX31_RS20175 0.43 -2.9 663,853 + EX31_RS20175 0.43 -2.2 663,853 + EX31_RS20175 0.43 -1.5 663,854 - EX31_RS20175 0.43 -3.6 663,854 - EX31_RS20175 0.43 -0.6 663,928 + EX31_RS20175 0.51 -2.6 663,928 + EX31_RS20175 0.51 -3.8 663,980 - EX31_RS20175 0.56 -1.6 663,980 - EX31_RS20175 0.56 -2.2 664,106 - EX31_RS20175 0.69 -2.8 664,132 + EX31_RS20175 0.72 -3.4 664,176 + EX31_RS20175 0.77 -2.6 664,374 + +0.2 664,375 - -2.5 664,400 + -1.2 664,400 + +0.0 664,400 + -0.2 664,414 + -0.1 664,415 - +0.5 664,471 + -1.0 664,471 + +1.5 664,471 + -1.6 664,472 - -1.0 664,472 - -2.5 664,472 - -0.6 664,472 - -0.8 664,506 + -2.6 664,507 - +0.1 664,507 - +0.5 664,510 - -0.0 664,510 - +0.2 664,513 + -1.4 664,514 - -0.9 664,612 + -0.3 664,612 + -1.4 664,613 - -2.3 664,623 + -0.3 664,624 - -2.1 664,624 - -0.9 664,734 - -1.6 664,740 + +0.4 664,741 - -1.6 664,754 + -1.1 664,755 - -2.5 664,759 + -3.0 664,766 + -2.9 664,766 + -0.6 664,766 + -2.9 664,780 + -2.0 664,780 + -0.6 664,780 + -2.6 664,780 + -2.1 664,780 + -3.4 664,780 + -1.1 664,780 + -1.9 664,780 + -2.8 664,780 + -3.0 664,780 + -2.6 664,781 - +0.9 664,781 - -1.4 664,781 - -0.6 664,781 - -0.6 664,785 + -2.1 664,785 + -3.6 664,785 + -1.8 664,785 + -3.1 664,786 - -1.1 664,788 + -2.9 664,788 + -2.5 664,788 + -3.1 664,789 - -1.6 664,789 - -0.6 664,789 - -2.4 664,823 + -1.9 664,823 + -1.6 664,823 + -1.6 664,823 + -3.4 664,826 + -0.1 664,826 + -3.7 664,826 + -2.5 664,826 + -1.6 664,829 + +0.9 664,838 + EX31_RS20180 0.12 -2.2 664,838 + EX31_RS20180 0.12 -2.9 664,937 + EX31_RS20180 0.51 -3.2 664,937 + EX31_RS20180 0.51 -1.2 665,038 + -0.6 665,061 + -0.8 665,061 + +0.1 665,065 - -1.6 665,065 - -0.6 665,078 + -0.1 665,078 + +0.4 665,078 + -0.4 665,081 - -2.9 665,107 + -1.1 665,107 + -2.5 665,107 + -0.4 665,113 + -2.1 665,382 + EX31_RS20185 0.16 -3.6 665,581 + EX31_RS20185 0.27 -3.0 665,650 + EX31_RS20185 0.31 -1.1 665,671 + EX31_RS20185 0.32 -2.6 665,672 - EX31_RS20185 0.32 -1.4 665,762 + EX31_RS20185 0.38 -2.5 665,762 + EX31_RS20185 0.38 -1.1 665,762 + EX31_RS20185 0.38 +0.9 665,763 - EX31_RS20185 0.38 -0.6 665,782 + EX31_RS20185 0.39 -2.2 665,795 + EX31_RS20185 0.39 -1.4 665,849 + EX31_RS20185 0.43 -1.4 665,849 + EX31_RS20185 0.43 -2.1 666,062 + EX31_RS20185 0.55 -2.6 666,062 + EX31_RS20185 0.55 +0.9
Or see this region's nucleotide sequence