Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20130 and EX31_RS20135 are separated by 287 nucleotides EX31_RS20135 and EX31_RS20140 are separated by 114 nucleotides EX31_RS20140 and EX31_RS20145 are separated by 32 nucleotides
EX31_RS20130: EX31_RS20130 - ethanolamine permease, at 654,035 to 655,444
_RS20130
EX31_RS20135: EX31_RS20135 - nucleotidyltransferase family protein, at 655,732 to 656,283
_RS20135
EX31_RS20140: EX31_RS20140 - hypothetical protein, at 656,398 to 656,739
_RS20140
EX31_RS20145: EX31_RS20145 - LysR family transcriptional regulator, at 656,772 to 657,698
_RS20145
Position (kb) 656
657 Strain fitness (log2 ratio) -2
-1
0
1
2 at 655.408 kb on + strand at 655.408 kb on + strand at 655.408 kb on + strand at 655.408 kb on + strand at 655.408 kb on + strand at 655.408 kb on + strand at 655.408 kb on + strand at 655.409 kb on - strand at 655.409 kb on - strand at 655.409 kb on - strand at 655.409 kb on - strand at 655.409 kb on - strand at 655.409 kb on - strand at 655.444 kb on + strand at 655.444 kb on + strand at 655.444 kb on + strand at 655.444 kb on + strand at 655.444 kb on + strand at 655.444 kb on + strand at 655.466 kb on + strand at 655.467 kb on - strand at 655.509 kb on + strand at 655.509 kb on + strand at 655.509 kb on + strand at 655.509 kb on + strand at 655.509 kb on + strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.510 kb on - strand at 655.546 kb on - strand at 655.552 kb on + strand at 655.552 kb on + strand at 655.552 kb on + strand at 655.553 kb on - strand at 655.553 kb on - strand at 655.553 kb on - strand at 655.603 kb on + strand at 655.624 kb on - strand at 655.674 kb on - strand at 655.913 kb on - strand, within EX31_RS20135 at 655.913 kb on - strand, within EX31_RS20135 at 656.044 kb on + strand, within EX31_RS20135 at 656.045 kb on - strand, within EX31_RS20135 at 656.071 kb on + strand, within EX31_RS20135 at 656.072 kb on - strand, within EX31_RS20135 at 656.175 kb on + strand, within EX31_RS20135 at 656.175 kb on + strand, within EX31_RS20135 at 656.176 kb on - strand, within EX31_RS20135 at 656.290 kb on + strand at 656.290 kb on + strand at 656.290 kb on + strand at 656.291 kb on - strand at 656.294 kb on + strand at 656.294 kb on + strand at 656.294 kb on + strand at 656.295 kb on - strand at 656.306 kb on + strand at 656.306 kb on + strand at 656.319 kb on + strand at 656.320 kb on - strand at 656.320 kb on - strand at 656.320 kb on - strand at 656.343 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.345 kb on + strand at 656.346 kb on - strand at 656.346 kb on - strand at 656.346 kb on - strand at 656.346 kb on - strand at 656.346 kb on - strand at 656.350 kb on + strand at 656.350 kb on + strand at 656.350 kb on + strand at 656.350 kb on + strand at 656.351 kb on - strand at 656.351 kb on - strand at 656.360 kb on + strand at 656.361 kb on - strand at 656.374 kb on + strand at 656.381 kb on - strand at 656.381 kb on - strand at 656.407 kb on - strand at 656.429 kb on + strand at 656.485 kb on - strand, within EX31_RS20140 at 656.554 kb on - strand, within EX31_RS20140 at 656.701 kb on - strand, within EX31_RS20140 at 656.701 kb on - strand, within EX31_RS20140 at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.731 kb on + strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.732 kb on - strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.735 kb on + strand at 656.736 kb on - strand at 656.736 kb on - strand at 656.736 kb on - strand at 656.737 kb on + strand at 656.737 kb on + strand at 656.738 kb on - strand at 656.770 kb on + strand at 656.770 kb on + strand at 656.782 kb on + strand at 656.782 kb on + strand at 656.782 kb on + strand at 656.782 kb on + strand at 656.782 kb on + strand at 656.782 kb on + strand at 656.783 kb on - strand at 656.783 kb on - strand at 656.783 kb on - strand at 656.783 kb on - strand at 656.783 kb on - strand at 656.811 kb on + strand at 656.814 kb on + strand at 656.815 kb on - strand at 656.879 kb on + strand, within EX31_RS20145 at 657.012 kb on + strand, within EX31_RS20145 at 657.012 kb on + strand, within EX31_RS20145 at 657.012 kb on + strand, within EX31_RS20145 at 657.106 kb on + strand, within EX31_RS20145 at 657.293 kb on + strand, within EX31_RS20145 at 657.329 kb on + strand, within EX31_RS20145 at 657.329 kb on + strand, within EX31_RS20145 at 657.365 kb on + strand, within EX31_RS20145 at 657.366 kb on - strand, within EX31_RS20145 at 657.366 kb on - strand, within EX31_RS20145 at 657.366 kb on - strand, within EX31_RS20145 at 657.401 kb on + strand, within EX31_RS20145 at 657.425 kb on + strand, within EX31_RS20145 at 657.426 kb on - strand, within EX31_RS20145 at 657.426 kb on - strand, within EX31_RS20145 at 657.426 kb on - strand, within EX31_RS20145 at 657.558 kb on + strand, within EX31_RS20145 at 657.558 kb on + strand, within EX31_RS20145 at 657.559 kb on - strand, within EX31_RS20145 at 657.587 kb on + strand, within EX31_RS20145 at 657.588 kb on - strand, within EX31_RS20145 at 657.588 kb on - strand, within EX31_RS20145 at 657.588 kb on - strand, within EX31_RS20145 at 657.588 kb on - strand, within EX31_RS20145 at 657.613 kb on - strand at 657.613 kb on - strand at 657.613 kb on - strand at 657.613 kb on - strand at 657.649 kb on + strand at 657.650 kb on - strand at 657.669 kb on + strand at 657.725 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 655,408 + -0.6 655,408 + +1.6 655,408 + -0.3 655,408 + +0.9 655,408 + +0.7 655,408 + +0.5 655,408 + -1.4 655,409 - -0.3 655,409 - +0.6 655,409 - +0.6 655,409 - -2.1 655,409 - +0.5 655,409 - -0.2 655,444 + -0.3 655,444 + +0.7 655,444 + +0.9 655,444 + +0.1 655,444 + +1.4 655,444 + +0.9 655,466 + +0.8 655,467 - +0.5 655,509 + -0.1 655,509 + +0.8 655,509 + -0.1 655,509 + -1.1 655,509 + +0.7 655,510 - +0.1 655,510 - +0.1 655,510 - -0.9 655,510 - -1.3 655,510 - +0.5 655,510 - -0.3 655,510 - -0.7 655,546 - -0.4 655,552 + +0.9 655,552 + -0.4 655,552 + +0.1 655,553 - +0.9 655,553 - +0.4 655,553 - -0.4 655,603 + -0.5 655,624 - -0.3 655,674 - +1.5 655,913 - EX31_RS20135 0.33 -2.0 655,913 - EX31_RS20135 0.33 +0.4 656,044 + EX31_RS20135 0.57 +2.6 656,045 - EX31_RS20135 0.57 +0.0 656,071 + EX31_RS20135 0.61 -0.1 656,072 - EX31_RS20135 0.62 -0.4 656,175 + EX31_RS20135 0.80 -0.1 656,175 + EX31_RS20135 0.80 -0.6 656,176 - EX31_RS20135 0.80 -0.6 656,290 + +0.0 656,290 + +0.8 656,290 + +1.1 656,291 - +0.5 656,294 + +0.0 656,294 + -0.4 656,294 + -0.6 656,295 - -1.9 656,306 + -0.4 656,306 + +0.6 656,319 + +0.7 656,320 - +1.3 656,320 - +0.9 656,320 - +0.1 656,343 + -0.6 656,345 + +2.3 656,345 + +2.3 656,345 + -0.9 656,345 + +1.3 656,345 + -0.1 656,345 + +0.9 656,345 + -0.4 656,345 + -0.4 656,345 + +0.3 656,346 - +0.7 656,346 - +2.1 656,346 - +0.6 656,346 - -0.1 656,346 - +2.5 656,350 + -0.4 656,350 + +0.1 656,350 + -1.3 656,350 + +0.8 656,351 - -0.1 656,351 - -0.5 656,360 + +0.4 656,361 - -0.9 656,374 + -0.1 656,381 - +0.3 656,381 - -1.4 656,407 - +0.1 656,429 + +0.9 656,485 - EX31_RS20140 0.25 -2.0 656,554 - EX31_RS20140 0.46 +1.4 656,701 - EX31_RS20140 0.89 -1.6 656,701 - EX31_RS20140 0.89 +0.9 656,731 + -0.6 656,731 + +0.9 656,731 + -0.1 656,731 + +0.4 656,731 + -2.1 656,731 + +0.1 656,731 + +0.2 656,731 + +0.0 656,731 + -2.5 656,731 + -1.3 656,731 + +0.1 656,731 + +0.9 656,731 + +0.9 656,731 + +0.6 656,731 + -0.2 656,731 + +1.1 656,731 + +0.5 656,731 + +0.5 656,731 + -1.2 656,732 - -1.0 656,732 - +1.4 656,732 - +0.5 656,732 - +2.5 656,732 - +0.8 656,732 - +2.5 656,732 - +0.7 656,732 - -1.4 656,732 - -1.1 656,732 - +0.6 656,732 - +2.4 656,735 + -0.3 656,735 + -0.8 656,735 + +0.4 656,735 + +0.9 656,735 + +1.4 656,735 + -0.6 656,735 + -1.1 656,735 + -1.1 656,735 + +0.9 656,735 + -0.6 656,735 + +1.1 656,735 + +0.7 656,735 + -0.4 656,735 + -0.3 656,735 + +0.0 656,735 + +0.3 656,735 + +0.4 656,736 - -1.5 656,736 - -0.3 656,736 - -0.8 656,737 + +0.9 656,737 + +1.9 656,738 - -0.6 656,770 + +0.9 656,770 + +1.9 656,782 + +0.6 656,782 + +0.9 656,782 + -0.6 656,782 + +0.1 656,782 + +0.8 656,782 + +1.7 656,783 - +0.9 656,783 - +1.3 656,783 - +1.9 656,783 - -0.2 656,783 - -1.2 656,811 + +1.4 656,814 + -0.1 656,815 - +1.4 656,879 + EX31_RS20145 0.12 -0.6 657,012 + EX31_RS20145 0.26 +0.9 657,012 + EX31_RS20145 0.26 +0.9 657,012 + EX31_RS20145 0.26 +0.9 657,106 + EX31_RS20145 0.36 -0.3 657,293 + EX31_RS20145 0.56 -0.6 657,329 + EX31_RS20145 0.60 +0.3 657,329 + EX31_RS20145 0.60 +0.8 657,365 + EX31_RS20145 0.64 -0.3 657,366 - EX31_RS20145 0.64 -0.8 657,366 - EX31_RS20145 0.64 -0.6 657,366 - EX31_RS20145 0.64 +0.0 657,401 + EX31_RS20145 0.68 -0.1 657,425 + EX31_RS20145 0.70 +0.2 657,426 - EX31_RS20145 0.71 +1.4 657,426 - EX31_RS20145 0.71 +1.2 657,426 - EX31_RS20145 0.71 +0.2 657,558 + EX31_RS20145 0.85 -0.6 657,558 + EX31_RS20145 0.85 +1.1 657,559 - EX31_RS20145 0.85 -0.2 657,587 + EX31_RS20145 0.88 +0.7 657,588 - EX31_RS20145 0.88 -0.6 657,588 - EX31_RS20145 0.88 -0.3 657,588 - EX31_RS20145 0.88 +0.2 657,588 - EX31_RS20145 0.88 -1.6 657,613 - +1.5 657,613 - -1.3 657,613 - -0.3 657,613 - +1.8 657,649 + +0.5 657,650 - +0.5 657,669 + -2.4 657,725 - +0.1
Or see this region's nucleotide sequence