Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18105 and EX31_RS18110 are separated by 59 nucleotides EX31_RS18110 and EX31_RS18115 are separated by 11 nucleotides EX31_RS18115 and EX31_RS18120 overlap by 4 nucleotides
EX31_RS18105: EX31_RS18105 - sulfonate ABC transporter substrate-binding protein, at 182,524 to 183,498
_RS18105
EX31_RS18110: EX31_RS18110 - FMNH2-dependent alkanesulfonate monooxygenase, at 183,558 to 184,712
_RS18110
EX31_RS18115: EX31_RS18115 - aliphatic sulfonate ABC transporter permease SsuC, at 184,724 to 185,515
_RS18115
EX31_RS18120: EX31_RS18120 - aliphatic sulfonates ABC transporter ATP-binding protein, at 185,512 to 186,294
_RS18120
Position (kb) 183
184
185 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 182.633 kb on + strand, within EX31_RS18105 at 182.634 kb on - strand, within EX31_RS18105 at 182.634 kb on - strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.638 kb on - strand, within EX31_RS18105 at 182.638 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.713 kb on - strand, within EX31_RS18105 at 182.720 kb on + strand, within EX31_RS18105 at 182.720 kb on + strand, within EX31_RS18105 at 182.730 kb on - strand, within EX31_RS18105 at 182.778 kb on + strand, within EX31_RS18105 at 182.778 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.847 kb on + strand, within EX31_RS18105 at 183.032 kb on - strand, within EX31_RS18105 at 183.091 kb on - strand, within EX31_RS18105 at 183.181 kb on + strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.289 kb on - strand, within EX31_RS18105 at 183.341 kb on - strand, within EX31_RS18105 at 183.361 kb on - strand, within EX31_RS18105 at 183.425 kb on - strand at 183.435 kb on + strand at 183.436 kb on - strand at 183.436 kb on - strand at 183.436 kb on - strand at 183.459 kb on + strand at 183.459 kb on + strand at 183.459 kb on + strand at 183.460 kb on - strand at 183.485 kb on + strand at 183.485 kb on + strand at 183.486 kb on - strand at 183.486 kb on - strand at 183.486 kb on - strand at 183.493 kb on - strand at 183.571 kb on + strand at 183.583 kb on + strand at 183.583 kb on + strand at 183.584 kb on - strand at 183.584 kb on - strand at 183.588 kb on - strand at 183.606 kb on + strand at 183.607 kb on - strand at 183.682 kb on + strand, within EX31_RS18110 at 183.682 kb on + strand, within EX31_RS18110 at 183.700 kb on + strand, within EX31_RS18110 at 183.700 kb on + strand, within EX31_RS18110 at 183.803 kb on + strand, within EX31_RS18110 at 183.804 kb on - strand, within EX31_RS18110 at 183.872 kb on + strand, within EX31_RS18110 at 183.919 kb on + strand, within EX31_RS18110 at 183.920 kb on - strand, within EX31_RS18110 at 183.920 kb on - strand, within EX31_RS18110 at 183.932 kb on + strand, within EX31_RS18110 at 183.932 kb on + strand, within EX31_RS18110 at 183.942 kb on + strand, within EX31_RS18110 at 183.968 kb on + strand, within EX31_RS18110 at 184.080 kb on + strand, within EX31_RS18110 at 184.081 kb on - strand, within EX31_RS18110 at 184.138 kb on - strand, within EX31_RS18110 at 184.319 kb on + strand, within EX31_RS18110 at 184.319 kb on + strand, within EX31_RS18110 at 184.320 kb on - strand, within EX31_RS18110 at 184.447 kb on + strand, within EX31_RS18110 at 184.447 kb on + strand, within EX31_RS18110 at 184.502 kb on + strand, within EX31_RS18110 at 184.502 kb on + strand, within EX31_RS18110 at 184.502 kb on + strand, within EX31_RS18110 at 184.503 kb on - strand, within EX31_RS18110 at 184.503 kb on - strand, within EX31_RS18110 at 184.512 kb on + strand, within EX31_RS18110 at 184.512 kb on + strand, within EX31_RS18110 at 184.513 kb on - strand, within EX31_RS18110 at 184.532 kb on + strand, within EX31_RS18110 at 184.533 kb on - strand, within EX31_RS18110 at 184.538 kb on + strand, within EX31_RS18110 at 184.572 kb on + strand, within EX31_RS18110 at 184.631 kb on + strand at 184.632 kb on - strand at 184.916 kb on - strand, within EX31_RS18115 at 185.138 kb on + strand, within EX31_RS18115 at 185.150 kb on + strand, within EX31_RS18115 at 185.227 kb on + strand, within EX31_RS18115 at 185.238 kb on + strand, within EX31_RS18115 at 185.238 kb on + strand, within EX31_RS18115 at 185.238 kb on + strand, within EX31_RS18115 at 185.238 kb on + strand, within EX31_RS18115 at 185.239 kb on - strand, within EX31_RS18115 at 185.263 kb on + strand, within EX31_RS18115 at 185.263 kb on + strand, within EX31_RS18115 at 185.349 kb on - strand, within EX31_RS18115 at 185.412 kb on - strand, within EX31_RS18115 at 185.667 kb on + strand, within EX31_RS18120 at 185.667 kb on + strand, within EX31_RS18120
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 182,633 + EX31_RS18105 0.11 -2.6 182,634 - EX31_RS18105 0.11 -1.2 182,634 - EX31_RS18105 0.11 +1.8 182,637 + EX31_RS18105 0.12 -1.1 182,637 + EX31_RS18105 0.12 -0.5 182,637 + EX31_RS18105 0.12 -0.8 182,638 - EX31_RS18105 0.12 -1.5 182,638 - EX31_RS18105 0.12 -1.5 182,645 - EX31_RS18105 0.12 -0.2 182,645 - EX31_RS18105 0.12 -2.2 182,645 - EX31_RS18105 0.12 +0.4 182,713 - EX31_RS18105 0.19 -0.8 182,720 + EX31_RS18105 0.20 -2.1 182,720 + EX31_RS18105 0.20 -1.2 182,730 - EX31_RS18105 0.21 -3.1 182,778 + EX31_RS18105 0.26 +0.8 182,778 + EX31_RS18105 0.26 +0.5 182,845 + EX31_RS18105 0.33 -0.5 182,845 + EX31_RS18105 0.33 +0.2 182,845 + EX31_RS18105 0.33 -0.8 182,845 + EX31_RS18105 0.33 -2.0 182,845 + EX31_RS18105 0.33 -0.1 182,845 + EX31_RS18105 0.33 -1.6 182,845 + EX31_RS18105 0.33 -1.7 182,845 + EX31_RS18105 0.33 -1.4 182,845 + EX31_RS18105 0.33 -2.0 182,846 - EX31_RS18105 0.33 +0.8 182,846 - EX31_RS18105 0.33 +0.4 182,846 - EX31_RS18105 0.33 +0.2 182,846 - EX31_RS18105 0.33 +0.0 182,847 + EX31_RS18105 0.33 -2.4 183,032 - EX31_RS18105 0.52 -0.5 183,091 - EX31_RS18105 0.58 -2.2 183,181 + EX31_RS18105 0.67 -0.8 183,182 - EX31_RS18105 0.67 +0.8 183,182 - EX31_RS18105 0.67 -2.0 183,182 - EX31_RS18105 0.67 +3.1 183,182 - EX31_RS18105 0.67 +0.4 183,289 - EX31_RS18105 0.78 -0.2 183,341 - EX31_RS18105 0.84 -1.3 183,361 - EX31_RS18105 0.86 -2.2 183,425 - -0.1 183,435 + -1.6 183,436 - -1.5 183,436 - +0.8 183,436 - -2.2 183,459 + -0.2 183,459 + -0.2 183,459 + -1.1 183,460 - -1.8 183,485 + -0.9 183,485 + -0.1 183,486 - -0.5 183,486 - +1.2 183,486 - -0.8 183,493 - -0.5 183,571 + -0.5 183,583 + -1.8 183,583 + -2.0 183,584 - -2.6 183,584 - -0.2 183,588 - +0.2 183,606 + -0.5 183,607 - +0.8 183,682 + EX31_RS18110 0.11 -1.3 183,682 + EX31_RS18110 0.11 -0.6 183,700 + EX31_RS18110 0.12 -1.2 183,700 + EX31_RS18110 0.12 +0.0 183,803 + EX31_RS18110 0.21 +0.3 183,804 - EX31_RS18110 0.21 -1.8 183,872 + EX31_RS18110 0.27 +0.1 183,919 + EX31_RS18110 0.31 -1.8 183,920 - EX31_RS18110 0.31 -0.1 183,920 - EX31_RS18110 0.31 -1.5 183,932 + EX31_RS18110 0.32 -1.5 183,932 + EX31_RS18110 0.32 -0.9 183,942 + EX31_RS18110 0.33 -1.2 183,968 + EX31_RS18110 0.35 -2.8 184,080 + EX31_RS18110 0.45 -0.5 184,081 - EX31_RS18110 0.45 -0.8 184,138 - EX31_RS18110 0.50 +1.8 184,319 + EX31_RS18110 0.66 -2.5 184,319 + EX31_RS18110 0.66 +0.8 184,320 - EX31_RS18110 0.66 -1.5 184,447 + EX31_RS18110 0.77 -0.8 184,447 + EX31_RS18110 0.77 -2.8 184,502 + EX31_RS18110 0.82 +0.4 184,502 + EX31_RS18110 0.82 -0.4 184,502 + EX31_RS18110 0.82 -0.5 184,503 - EX31_RS18110 0.82 -2.2 184,503 - EX31_RS18110 0.82 -0.6 184,512 + EX31_RS18110 0.83 -0.8 184,512 + EX31_RS18110 0.83 -1.8 184,513 - EX31_RS18110 0.83 -0.8 184,532 + EX31_RS18110 0.84 -1.5 184,533 - EX31_RS18110 0.84 -1.2 184,538 + EX31_RS18110 0.85 -1.4 184,572 + EX31_RS18110 0.88 -1.8 184,631 + -0.2 184,632 - -0.6 184,916 - EX31_RS18115 0.24 -0.2 185,138 + EX31_RS18115 0.52 -0.8 185,150 + EX31_RS18115 0.54 -0.3 185,227 + EX31_RS18115 0.64 +0.8 185,238 + EX31_RS18115 0.65 -0.8 185,238 + EX31_RS18115 0.65 +0.2 185,238 + EX31_RS18115 0.65 -0.4 185,238 + EX31_RS18115 0.65 -2.0 185,239 - EX31_RS18115 0.65 -3.3 185,263 + EX31_RS18115 0.68 +0.7 185,263 + EX31_RS18115 0.68 -1.8 185,349 - EX31_RS18115 0.79 -0.8 185,412 - EX31_RS18115 0.87 -1.0 185,667 + EX31_RS18120 0.20 -2.2 185,667 + EX31_RS18120 0.20 -0.2
Or see this region's nucleotide sequence