Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18095 and EX31_RS18100 are separated by 315 nucleotides EX31_RS18100 and EX31_RS18105 are separated by 18 nucleotides
EX31_RS18095: EX31_RS18095 - quinone-dependent dihydroorotate dehydrogenase, at 180,598 to 181,608
_RS18095
EX31_RS18100: EX31_RS18100 - NADPH-dependent FMN reductase, at 181,924 to 182,505
_RS18100
EX31_RS18105: EX31_RS18105 - sulfonate ABC transporter substrate-binding protein, at 182,524 to 183,498
_RS18105
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.978 kb on - strand, within EX31_RS18095 at 180.978 kb on - strand, within EX31_RS18095 at 180.997 kb on + strand, within EX31_RS18095 at 180.998 kb on - strand, within EX31_RS18095 at 181.067 kb on - strand, within EX31_RS18095 at 181.105 kb on - strand, within EX31_RS18095 at 181.105 kb on - strand, within EX31_RS18095 at 181.123 kb on + strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.134 kb on + strand, within EX31_RS18095 at 181.135 kb on - strand, within EX31_RS18095 at 181.135 kb on - strand, within EX31_RS18095 at 181.145 kb on + strand, within EX31_RS18095 at 181.173 kb on + strand, within EX31_RS18095 at 181.173 kb on + strand, within EX31_RS18095 at 181.180 kb on - strand, within EX31_RS18095 at 181.192 kb on - strand, within EX31_RS18095 at 181.195 kb on - strand, within EX31_RS18095 at 181.233 kb on + strand, within EX31_RS18095 at 181.234 kb on - strand, within EX31_RS18095 at 181.310 kb on - strand, within EX31_RS18095 at 181.320 kb on + strand, within EX31_RS18095 at 181.320 kb on + strand, within EX31_RS18095 at 181.398 kb on + strand, within EX31_RS18095 at 181.399 kb on - strand, within EX31_RS18095 at 181.399 kb on - strand, within EX31_RS18095 at 181.447 kb on - strand, within EX31_RS18095 at 181.546 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.605 kb on - strand at 181.605 kb on - strand at 181.634 kb on - strand at 181.646 kb on - strand at 181.655 kb on + strand at 181.667 kb on - strand at 181.686 kb on + strand at 181.689 kb on - strand at 181.734 kb on + strand at 181.751 kb on + strand at 181.797 kb on + strand at 181.797 kb on + strand at 181.798 kb on - strand at 181.858 kb on + strand at 181.889 kb on + strand at 181.895 kb on + strand at 181.895 kb on + strand at 181.897 kb on + strand at 181.911 kb on - strand at 181.931 kb on + strand at 182.029 kb on - strand, within EX31_RS18100 at 182.033 kb on - strand, within EX31_RS18100 at 182.033 kb on - strand, within EX31_RS18100 at 182.037 kb on + strand, within EX31_RS18100 at 182.037 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.253 kb on + strand, within EX31_RS18100 at 182.253 kb on + strand, within EX31_RS18100 at 182.254 kb on - strand, within EX31_RS18100 at 182.262 kb on + strand, within EX31_RS18100 at 182.363 kb on - strand, within EX31_RS18100 at 182.509 kb on + strand at 182.519 kb on + strand at 182.519 kb on + strand at 182.520 kb on - strand at 182.520 kb on - strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.547 kb on - strand at 182.633 kb on + strand, within EX31_RS18105 at 182.634 kb on - strand, within EX31_RS18105 at 182.634 kb on - strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.637 kb on + strand, within EX31_RS18105 at 182.638 kb on - strand, within EX31_RS18105 at 182.638 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.645 kb on - strand, within EX31_RS18105 at 182.713 kb on - strand, within EX31_RS18105 at 182.720 kb on + strand, within EX31_RS18105 at 182.720 kb on + strand, within EX31_RS18105 at 182.730 kb on - strand, within EX31_RS18105 at 182.778 kb on + strand, within EX31_RS18105 at 182.778 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.845 kb on + strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.846 kb on - strand, within EX31_RS18105 at 182.847 kb on + strand, within EX31_RS18105 at 183.032 kb on - strand, within EX31_RS18105 at 183.091 kb on - strand, within EX31_RS18105 at 183.181 kb on + strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.182 kb on - strand, within EX31_RS18105 at 183.289 kb on - strand, within EX31_RS18105 at 183.341 kb on - strand, within EX31_RS18105 at 183.361 kb on - strand, within EX31_RS18105 at 183.425 kb on - strand at 183.435 kb on + strand at 183.436 kb on - strand at 183.436 kb on - strand at 183.436 kb on - strand at 183.459 kb on + strand at 183.459 kb on + strand at 183.459 kb on + strand at 183.460 kb on - strand at 183.485 kb on + strand at 183.485 kb on + strand at 183.486 kb on - strand at 183.486 kb on - strand at 183.486 kb on - strand at 183.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 180,978 - EX31_RS18095 0.38 -2.5 180,978 - EX31_RS18095 0.38 -2.6 180,997 + EX31_RS18095 0.39 -1.2 180,998 - EX31_RS18095 0.40 -1.2 181,067 - EX31_RS18095 0.46 -2.6 181,105 - EX31_RS18095 0.50 -0.2 181,105 - EX31_RS18095 0.50 -2.2 181,123 + EX31_RS18095 0.52 -1.2 181,124 - EX31_RS18095 0.52 -2.0 181,124 - EX31_RS18095 0.52 -2.5 181,124 - EX31_RS18095 0.52 -0.8 181,134 + EX31_RS18095 0.53 -2.5 181,135 - EX31_RS18095 0.53 -1.8 181,135 - EX31_RS18095 0.53 -2.0 181,145 + EX31_RS18095 0.54 +0.8 181,173 + EX31_RS18095 0.57 -2.2 181,173 + EX31_RS18095 0.57 -2.2 181,180 - EX31_RS18095 0.58 -1.2 181,192 - EX31_RS18095 0.59 -3.3 181,195 - EX31_RS18095 0.59 -2.2 181,233 + EX31_RS18095 0.63 -2.5 181,234 - EX31_RS18095 0.63 -2.4 181,310 - EX31_RS18095 0.70 -1.5 181,320 + EX31_RS18095 0.71 -0.2 181,320 + EX31_RS18095 0.71 -1.2 181,398 + EX31_RS18095 0.79 -3.2 181,399 - EX31_RS18095 0.79 -2.9 181,399 - EX31_RS18095 0.79 +0.8 181,447 - EX31_RS18095 0.84 -1.8 181,546 + +0.8 181,604 + -1.5 181,604 + -1.5 181,604 + -0.8 181,604 + +0.8 181,605 - -2.9 181,605 - -3.3 181,634 - -0.5 181,646 - -0.9 181,655 + -0.8 181,667 - -2.6 181,686 + -1.4 181,689 - +0.5 181,734 + +1.1 181,751 + +0.3 181,797 + -0.2 181,797 + -0.6 181,798 - +0.3 181,858 + -0.2 181,889 + -0.2 181,895 + +0.8 181,895 + -0.2 181,897 + -2.4 181,911 - -2.2 181,931 + -0.2 182,029 - EX31_RS18100 0.18 -0.9 182,033 - EX31_RS18100 0.19 -1.4 182,033 - EX31_RS18100 0.19 +0.2 182,037 + EX31_RS18100 0.19 +2.4 182,037 + EX31_RS18100 0.19 -1.1 182,152 + EX31_RS18100 0.39 -1.9 182,152 + EX31_RS18100 0.39 -0.2 182,152 + EX31_RS18100 0.39 +0.8 182,153 - EX31_RS18100 0.39 -0.2 182,153 - EX31_RS18100 0.39 +0.8 182,153 - EX31_RS18100 0.39 +1.2 182,153 - EX31_RS18100 0.39 -0.2 182,154 + EX31_RS18100 0.40 +0.8 182,154 + EX31_RS18100 0.40 -0.9 182,154 + EX31_RS18100 0.40 -0.2 182,154 + EX31_RS18100 0.40 -0.0 182,253 + EX31_RS18100 0.57 -0.6 182,253 + EX31_RS18100 0.57 -0.4 182,254 - EX31_RS18100 0.57 +0.8 182,262 + EX31_RS18100 0.58 +1.8 182,363 - EX31_RS18100 0.75 +0.1 182,509 + -0.2 182,519 + -1.5 182,519 + +1.1 182,520 - -0.2 182,520 - -0.8 182,543 + -0.1 182,543 + +0.1 182,543 + +3.1 182,543 + -0.6 182,547 - +1.8 182,633 + EX31_RS18105 0.11 -2.6 182,634 - EX31_RS18105 0.11 -1.2 182,634 - EX31_RS18105 0.11 +1.8 182,637 + EX31_RS18105 0.12 -1.1 182,637 + EX31_RS18105 0.12 -0.5 182,637 + EX31_RS18105 0.12 -0.8 182,638 - EX31_RS18105 0.12 -1.5 182,638 - EX31_RS18105 0.12 -1.5 182,645 - EX31_RS18105 0.12 -0.2 182,645 - EX31_RS18105 0.12 -2.2 182,645 - EX31_RS18105 0.12 +0.4 182,713 - EX31_RS18105 0.19 -0.8 182,720 + EX31_RS18105 0.20 -2.1 182,720 + EX31_RS18105 0.20 -1.2 182,730 - EX31_RS18105 0.21 -3.1 182,778 + EX31_RS18105 0.26 +0.8 182,778 + EX31_RS18105 0.26 +0.5 182,845 + EX31_RS18105 0.33 -0.5 182,845 + EX31_RS18105 0.33 +0.2 182,845 + EX31_RS18105 0.33 -0.8 182,845 + EX31_RS18105 0.33 -2.0 182,845 + EX31_RS18105 0.33 -0.1 182,845 + EX31_RS18105 0.33 -1.6 182,845 + EX31_RS18105 0.33 -1.7 182,845 + EX31_RS18105 0.33 -1.4 182,845 + EX31_RS18105 0.33 -2.0 182,846 - EX31_RS18105 0.33 +0.8 182,846 - EX31_RS18105 0.33 +0.4 182,846 - EX31_RS18105 0.33 +0.2 182,846 - EX31_RS18105 0.33 +0.0 182,847 + EX31_RS18105 0.33 -2.4 183,032 - EX31_RS18105 0.52 -0.5 183,091 - EX31_RS18105 0.58 -2.2 183,181 + EX31_RS18105 0.67 -0.8 183,182 - EX31_RS18105 0.67 +0.8 183,182 - EX31_RS18105 0.67 -2.0 183,182 - EX31_RS18105 0.67 +3.1 183,182 - EX31_RS18105 0.67 +0.4 183,289 - EX31_RS18105 0.78 -0.2 183,341 - EX31_RS18105 0.84 -1.3 183,361 - EX31_RS18105 0.86 -2.2 183,425 - -0.1 183,435 + -1.6 183,436 - -1.5 183,436 - +0.8 183,436 - -2.2 183,459 + -0.2 183,459 + -0.2 183,459 + -1.1 183,460 - -1.8 183,485 + -0.9 183,485 + -0.1 183,486 - -0.5 183,486 - +1.2 183,486 - -0.8 183,493 - -0.5
Or see this region's nucleotide sequence