Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11465 and EX31_RS11470 are separated by 23 nucleotides EX31_RS11470 and EX31_RS11475 are separated by 118 nucleotides EX31_RS11475 and EX31_RS11480 are separated by 503 nucleotides
EX31_RS11465: EX31_RS11465 - imidazole glycerol phosphate synthase subunit HisF, at 226,861 to 227,634
_RS11465
EX31_RS11470: EX31_RS11470 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 227,658 to 228,272
_RS11470
EX31_RS11475: EX31_RS11475 - NADP-dependent phosphogluconate dehydrogenase, at 228,391 to 229,800
_RS11475
EX31_RS11480: EX31_RS11480 - ABC transporter permease, at 230,304 to 231,122
_RS11480
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 227.391 kb on + strand, within EX31_RS11465 at 227.391 kb on + strand, within EX31_RS11465 at 227.574 kb on + strand at 227.574 kb on + strand at 227.599 kb on + strand at 227.600 kb on - strand at 227.647 kb on + strand at 227.647 kb on + strand at 227.647 kb on + strand at 227.648 kb on - strand at 227.763 kb on + strand, within EX31_RS11470 at 227.763 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.766 kb on - strand, within EX31_RS11470 at 227.810 kb on - strand, within EX31_RS11470 at 228.057 kb on + strand, within EX31_RS11470 at 228.059 kb on + strand, within EX31_RS11470 at 228.270 kb on + strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.392 kb on + strand at 228.393 kb on - strand at 228.393 kb on - strand at 228.416 kb on - strand at 228.416 kb on - strand at 228.441 kb on - strand at 228.441 kb on - strand at 228.901 kb on - strand, within EX31_RS11475 at 229.063 kb on - strand, within EX31_RS11475 at 229.063 kb on - strand, within EX31_RS11475 at 229.930 kb on + strand at 229.931 kb on - strand at 229.947 kb on + strand at 229.948 kb on - strand at 229.948 kb on - strand at 229.948 kb on - strand at 229.948 kb on - strand at 229.948 kb on - strand at 230.003 kb on + strand at 230.003 kb on + strand at 230.003 kb on + strand at 230.004 kb on - strand at 230.086 kb on + strand at 230.103 kb on - strand at 230.113 kb on + strand at 230.113 kb on + strand at 230.114 kb on - strand at 230.114 kb on - strand at 230.114 kb on - strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.115 kb on + strand at 230.116 kb on - strand at 230.116 kb on - strand at 230.116 kb on - strand at 230.116 kb on - strand at 230.116 kb on - strand at 230.122 kb on - strand at 230.150 kb on + strand at 230.157 kb on - strand at 230.178 kb on + strand at 230.178 kb on + strand at 230.179 kb on - strand at 230.179 kb on - strand at 230.179 kb on - strand at 230.179 kb on - strand at 230.179 kb on - strand at 230.187 kb on + strand at 230.187 kb on + strand at 230.187 kb on + strand at 230.188 kb on - strand at 230.194 kb on + strand at 230.212 kb on - strand at 230.242 kb on + strand at 230.242 kb on + strand at 230.245 kb on + strand at 230.245 kb on + strand at 230.245 kb on + strand at 230.246 kb on - strand at 230.255 kb on + strand at 230.257 kb on + strand at 230.257 kb on + strand at 230.257 kb on + strand at 230.257 kb on + strand at 230.257 kb on + strand at 230.258 kb on - strand at 230.258 kb on - strand at 230.260 kb on + strand at 230.260 kb on + strand at 230.260 kb on + strand at 230.260 kb on + strand at 230.261 kb on - strand at 230.261 kb on - strand at 230.261 kb on - strand at 230.261 kb on - strand at 230.268 kb on + strand at 230.268 kb on + strand at 230.268 kb on + strand at 230.280 kb on + strand at 230.280 kb on + strand at 230.287 kb on + strand at 230.287 kb on + strand at 230.288 kb on - strand at 230.294 kb on + strand at 230.294 kb on + strand at 230.294 kb on + strand at 230.295 kb on - strand at 230.350 kb on + strand at 230.366 kb on - strand at 230.372 kb on + strand at 230.405 kb on + strand, within EX31_RS11480 at 230.425 kb on + strand, within EX31_RS11480 at 230.426 kb on - strand, within EX31_RS11480 at 230.449 kb on + strand, within EX31_RS11480 at 230.453 kb on + strand, within EX31_RS11480 at 230.453 kb on + strand, within EX31_RS11480 at 230.468 kb on + strand, within EX31_RS11480 at 230.468 kb on + strand, within EX31_RS11480 at 230.488 kb on + strand, within EX31_RS11480 at 230.488 kb on + strand, within EX31_RS11480 at 230.492 kb on + strand, within EX31_RS11480 at 230.492 kb on + strand, within EX31_RS11480 at 230.540 kb on + strand, within EX31_RS11480 at 230.540 kb on + strand, within EX31_RS11480 at 230.540 kb on + strand, within EX31_RS11480 at 230.540 kb on + strand, within EX31_RS11480 at 230.542 kb on + strand, within EX31_RS11480 at 230.542 kb on + strand, within EX31_RS11480 at 230.621 kb on + strand, within EX31_RS11480 at 230.621 kb on + strand, within EX31_RS11480 at 230.621 kb on + strand, within EX31_RS11480 at 230.755 kb on + strand, within EX31_RS11480 at 230.791 kb on + strand, within EX31_RS11480 at 230.791 kb on + strand, within EX31_RS11480
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 227,391 + EX31_RS11465 0.68 -1.8 227,391 + EX31_RS11465 0.68 -3.4 227,574 + -1.8 227,574 + +0.8 227,599 + -2.2 227,600 - -1.5 227,647 + -3.4 227,647 + -0.8 227,647 + -2.0 227,648 - +0.8 227,763 + EX31_RS11470 0.17 -1.4 227,763 + EX31_RS11470 0.17 -0.8 227,765 + EX31_RS11470 0.17 -1.8 227,765 + EX31_RS11470 0.17 -2.2 227,765 + EX31_RS11470 0.17 -2.6 227,766 - EX31_RS11470 0.18 -1.5 227,810 - EX31_RS11470 0.25 +0.8 228,057 + EX31_RS11470 0.65 -1.2 228,059 + EX31_RS11470 0.65 -3.4 228,270 + +0.7 228,371 - -0.5 228,371 - -1.2 228,371 - -1.8 228,371 - -0.1 228,392 + +2.4 228,393 - -1.0 228,393 - -0.6 228,416 - -0.8 228,416 - -1.5 228,441 - -0.2 228,441 - -0.8 228,901 - EX31_RS11475 0.36 -0.2 229,063 - EX31_RS11475 0.48 -1.2 229,063 - EX31_RS11475 0.48 -1.8 229,930 + +0.2 229,931 - +0.8 229,947 + +0.1 229,948 - +0.3 229,948 - +0.4 229,948 - +0.6 229,948 - -1.1 229,948 - +1.8 230,003 + -0.8 230,003 + -0.2 230,003 + +0.8 230,004 - -1.5 230,086 + -1.8 230,103 - -1.4 230,113 + -0.8 230,113 + -0.3 230,114 - -1.1 230,114 - -0.1 230,114 - -0.8 230,115 + -0.3 230,115 + +0.5 230,115 + +0.8 230,115 + +1.4 230,115 + -1.2 230,115 + -2.2 230,115 + -0.2 230,115 + +0.1 230,115 + -0.2 230,115 + -0.5 230,115 + +0.4 230,116 - -2.0 230,116 - +0.8 230,116 - -0.8 230,116 - +0.8 230,116 - -0.4 230,122 - +0.2 230,150 + -0.6 230,157 - -1.3 230,178 + -1.2 230,178 + +0.1 230,179 - -0.2 230,179 - +0.4 230,179 - +0.5 230,179 - -0.3 230,179 - -0.5 230,187 + +0.8 230,187 + +1.1 230,187 + -1.8 230,188 - -0.1 230,194 + -0.9 230,212 - +2.4 230,242 + -0.2 230,242 + -1.6 230,245 + +0.7 230,245 + +0.1 230,245 + -0.6 230,246 - -1.2 230,255 + -0.8 230,257 + -1.4 230,257 + -1.8 230,257 + -0.1 230,257 + -2.5 230,257 + -0.5 230,258 - -2.0 230,258 - -0.2 230,260 + -1.5 230,260 + -1.9 230,260 + +0.0 230,260 + +0.4 230,261 - -1.2 230,261 - -1.7 230,261 - -0.4 230,261 - +2.0 230,268 + +0.4 230,268 + +0.0 230,268 + -0.9 230,280 + +0.1 230,280 + +0.8 230,287 + +1.0 230,287 + -0.8 230,288 - -0.5 230,294 + -1.0 230,294 + -0.3 230,294 + +1.8 230,295 - -0.2 230,350 + +2.0 230,366 - -1.5 230,372 + +2.1 230,405 + EX31_RS11480 0.12 +0.7 230,425 + EX31_RS11480 0.15 +0.8 230,426 - EX31_RS11480 0.15 -0.2 230,449 + EX31_RS11480 0.18 +1.5 230,453 + EX31_RS11480 0.18 +0.8 230,453 + EX31_RS11480 0.18 +0.4 230,468 + EX31_RS11480 0.20 +0.8 230,468 + EX31_RS11480 0.20 -1.7 230,488 + EX31_RS11480 0.22 -1.5 230,488 + EX31_RS11480 0.22 +0.0 230,492 + EX31_RS11480 0.23 +1.1 230,492 + EX31_RS11480 0.23 -0.8 230,540 + EX31_RS11480 0.29 +0.7 230,540 + EX31_RS11480 0.29 -0.4 230,540 + EX31_RS11480 0.29 -0.2 230,540 + EX31_RS11480 0.29 -0.5 230,542 + EX31_RS11480 0.29 +0.8 230,542 + EX31_RS11480 0.29 +1.4 230,621 + EX31_RS11480 0.39 +0.6 230,621 + EX31_RS11480 0.39 -0.2 230,621 + EX31_RS11480 0.39 +0.2 230,755 + EX31_RS11480 0.55 -0.9 230,791 + EX31_RS11480 0.59 +0.8 230,791 + EX31_RS11480 0.59 +1.6
Or see this region's nucleotide sequence