Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07330 and EX31_RS07335 are separated by 162 nucleotides EX31_RS07335 and EX31_RS07340 are separated by 122 nucleotides EX31_RS07340 and EX31_RS07345 are separated by 307 nucleotides
EX31_RS07330: EX31_RS07330 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 269,003 to 269,866
_RS07330
EX31_RS07335: EX31_RS07335 - ribose-phosphate pyrophosphokinase, at 270,029 to 270,976
_RS07335
EX31_RS07340: EX31_RS07340 - stress-induced protein YchH, at 271,099 to 271,377
_RS07340
EX31_RS07345: EX31_RS07345 - aminoacyl-tRNA hydrolase, at 271,685 to 272,260
_RS07345
Position (kb) 270
271 Strain fitness (log2 ratio) -2
-1
0
1
2 at 270.981 kb on + strand at 271.095 kb on - strand at 271.160 kb on + strand, within EX31_RS07340 at 271.160 kb on + strand, within EX31_RS07340 at 271.234 kb on - strand, within EX31_RS07340 at 271.378 kb on - strand at 271.378 kb on - strand at 271.404 kb on + strand at 271.405 kb on - strand at 271.438 kb on - strand at 271.556 kb on - strand at 271.601 kb on + strand at 271.601 kb on + strand at 271.601 kb on + strand at 271.628 kb on - strand at 271.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 270,981 + -0.2 271,095 - -0.3 271,160 + EX31_RS07340 0.22 -0.9 271,160 + EX31_RS07340 0.22 +1.9 271,234 - EX31_RS07340 0.48 +2.3 271,378 - -0.5 271,378 - -1.9 271,404 + -1.9 271,405 - -1.3 271,438 - +0.7 271,556 - -0.9 271,601 + +0.7 271,601 + +0.7 271,601 + +2.5 271,628 - +0.3 271,632 + +0.7
Or see this region's nucleotide sequence