Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04205 and EX31_RS04210 are separated by 66 nucleotides EX31_RS04210 and EX31_RS04215 are separated by 144 nucleotides
EX31_RS04205: EX31_RS04205 - hypothetical protein, at 365,843 to 366,574
_RS04205
EX31_RS04210: EX31_RS04210 - DAK2 domain-containing protein, at 366,641 to 368,293
_RS04210
EX31_RS04215: EX31_RS04215 - L-serine ammonia-lyase, at 368,438 to 369,805
_RS04215
Position (kb) 366
367
368
369 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 365.684 kb on + strand at 365.685 kb on - strand at 365.709 kb on + strand at 365.709 kb on + strand at 365.709 kb on + strand at 365.709 kb on + strand at 365.709 kb on + strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.710 kb on - strand at 365.711 kb on + strand at 365.711 kb on + strand at 365.712 kb on - strand at 365.776 kb on + strand at 365.777 kb on - strand at 365.779 kb on - strand at 365.787 kb on + strand at 365.802 kb on + strand at 365.802 kb on + strand at 365.802 kb on + strand at 365.803 kb on - strand at 365.803 kb on - strand at 365.803 kb on - strand at 365.803 kb on - strand at 365.803 kb on - strand at 365.803 kb on - strand at 365.825 kb on + strand at 365.836 kb on + strand at 365.842 kb on + strand at 365.849 kb on - strand at 365.852 kb on - strand at 365.868 kb on + strand at 365.868 kb on + strand at 365.875 kb on - strand at 365.993 kb on + strand, within EX31_RS04205 at 366.161 kb on + strand, within EX31_RS04205 at 366.161 kb on + strand, within EX31_RS04205 at 366.162 kb on - strand, within EX31_RS04205 at 366.162 kb on - strand, within EX31_RS04205 at 366.162 kb on - strand, within EX31_RS04205 at 366.162 kb on - strand, within EX31_RS04205 at 366.163 kb on + strand, within EX31_RS04205 at 366.164 kb on - strand, within EX31_RS04205 at 366.232 kb on + strand, within EX31_RS04205 at 366.259 kb on - strand, within EX31_RS04205 at 366.259 kb on - strand, within EX31_RS04205 at 366.259 kb on - strand, within EX31_RS04205 at 366.262 kb on - strand, within EX31_RS04205 at 366.279 kb on + strand, within EX31_RS04205 at 366.279 kb on + strand, within EX31_RS04205 at 366.280 kb on - strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.283 kb on + strand, within EX31_RS04205 at 366.284 kb on - strand, within EX31_RS04205 at 366.284 kb on - strand, within EX31_RS04205 at 366.284 kb on - strand, within EX31_RS04205 at 366.284 kb on - strand, within EX31_RS04205 at 366.284 kb on - strand, within EX31_RS04205 at 366.356 kb on + strand, within EX31_RS04205 at 366.357 kb on - strand, within EX31_RS04205 at 366.357 kb on - strand, within EX31_RS04205 at 366.385 kb on + strand, within EX31_RS04205 at 366.394 kb on + strand, within EX31_RS04205 at 366.395 kb on - strand, within EX31_RS04205 at 366.431 kb on + strand, within EX31_RS04205 at 366.431 kb on + strand, within EX31_RS04205 at 366.487 kb on + strand, within EX31_RS04205 at 366.491 kb on + strand, within EX31_RS04205 at 366.491 kb on + strand, within EX31_RS04205 at 366.492 kb on - strand, within EX31_RS04205 at 366.492 kb on - strand, within EX31_RS04205 at 366.532 kb on + strand at 366.532 kb on + strand at 366.532 kb on + strand at 366.542 kb on + strand at 366.568 kb on + strand at 366.568 kb on + strand at 366.568 kb on + strand at 366.569 kb on - strand at 366.726 kb on + strand at 366.727 kb on - strand at 366.727 kb on - strand at 366.727 kb on - strand at 366.889 kb on - strand, within EX31_RS04210 at 366.889 kb on - strand, within EX31_RS04210 at 366.889 kb on - strand, within EX31_RS04210 at 366.955 kb on + strand, within EX31_RS04210 at 366.955 kb on + strand, within EX31_RS04210 at 366.956 kb on - strand, within EX31_RS04210 at 366.956 kb on - strand, within EX31_RS04210 at 366.956 kb on - strand, within EX31_RS04210 at 367.054 kb on + strand, within EX31_RS04210 at 367.054 kb on + strand, within EX31_RS04210 at 367.055 kb on - strand, within EX31_RS04210 at 367.250 kb on - strand, within EX31_RS04210 at 367.391 kb on - strand, within EX31_RS04210 at 367.573 kb on + strand, within EX31_RS04210 at 367.574 kb on - strand, within EX31_RS04210 at 367.633 kb on + strand, within EX31_RS04210 at 367.633 kb on + strand, within EX31_RS04210 at 367.633 kb on + strand, within EX31_RS04210 at 367.634 kb on - strand, within EX31_RS04210 at 367.638 kb on - strand, within EX31_RS04210 at 367.638 kb on - strand, within EX31_RS04210 at 367.729 kb on + strand, within EX31_RS04210 at 367.730 kb on - strand, within EX31_RS04210 at 367.786 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.816 kb on + strand, within EX31_RS04210 at 367.817 kb on - strand, within EX31_RS04210 at 367.817 kb on - strand, within EX31_RS04210 at 367.817 kb on - strand, within EX31_RS04210 at 367.903 kb on + strand, within EX31_RS04210 at 367.903 kb on + strand, within EX31_RS04210 at 367.903 kb on + strand, within EX31_RS04210 at 367.904 kb on - strand, within EX31_RS04210 at 367.905 kb on + strand, within EX31_RS04210 at 367.905 kb on + strand, within EX31_RS04210 at 367.905 kb on + strand, within EX31_RS04210 at 367.906 kb on - strand, within EX31_RS04210 at 367.906 kb on - strand, within EX31_RS04210 at 367.906 kb on - strand, within EX31_RS04210 at 367.906 kb on - strand, within EX31_RS04210 at 367.910 kb on + strand, within EX31_RS04210 at 367.910 kb on + strand, within EX31_RS04210 at 367.911 kb on - strand, within EX31_RS04210 at 367.962 kb on + strand, within EX31_RS04210 at 367.963 kb on - strand, within EX31_RS04210 at 368.153 kb on - strand at 368.245 kb on + strand at 368.245 kb on + strand at 368.246 kb on - strand at 368.246 kb on - strand at 368.254 kb on + strand at 368.278 kb on + strand at 368.287 kb on + strand at 368.288 kb on - strand at 368.310 kb on + strand at 368.340 kb on + strand at 368.341 kb on - strand at 368.341 kb on - strand at 368.341 kb on - strand at 368.341 kb on - strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.349 kb on + strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.350 kb on - strand at 368.353 kb on + strand at 368.354 kb on - strand at 368.354 kb on - strand at 368.365 kb on + strand at 368.365 kb on + strand at 368.365 kb on + strand at 368.366 kb on - strand at 368.380 kb on + strand at 368.380 kb on + strand at 368.381 kb on - strand at 368.416 kb on + strand at 368.520 kb on + strand at 368.520 kb on + strand at 368.520 kb on + strand at 368.521 kb on - strand at 368.521 kb on - strand at 368.539 kb on + strand at 368.573 kb on + strand at 368.573 kb on + strand at 368.574 kb on - strand at 368.581 kb on + strand, within EX31_RS04215 at 368.708 kb on - strand, within EX31_RS04215 at 368.761 kb on + strand, within EX31_RS04215 at 368.761 kb on + strand, within EX31_RS04215 at 368.762 kb on - strand, within EX31_RS04215 at 368.762 kb on - strand, within EX31_RS04215 at 368.762 kb on - strand, within EX31_RS04215 at 368.836 kb on + strand, within EX31_RS04215 at 368.837 kb on - strand, within EX31_RS04215 at 368.907 kb on + strand, within EX31_RS04215 at 368.907 kb on + strand, within EX31_RS04215 at 368.907 kb on + strand, within EX31_RS04215 at 368.907 kb on + strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.908 kb on - strand, within EX31_RS04215 at 368.910 kb on + strand, within EX31_RS04215 at 368.910 kb on + strand, within EX31_RS04215 at 368.911 kb on - strand, within EX31_RS04215 at 368.911 kb on - strand, within EX31_RS04215 at 368.911 kb on - strand, within EX31_RS04215 at 368.911 kb on - strand, within EX31_RS04215 at 368.911 kb on - strand, within EX31_RS04215 at 369.006 kb on + strand, within EX31_RS04215 at 369.006 kb on + strand, within EX31_RS04215 at 369.006 kb on + strand, within EX31_RS04215 at 369.006 kb on + strand, within EX31_RS04215 at 369.006 kb on + strand, within EX31_RS04215 at 369.007 kb on - strand, within EX31_RS04215 at 369.007 kb on - strand, within EX31_RS04215 at 369.262 kb on + strand, within EX31_RS04215 at 369.262 kb on + strand, within EX31_RS04215
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 365,684 + +0.3 365,685 - -0.2 365,709 + -0.5 365,709 + -1.1 365,709 + -0.1 365,709 + -1.0 365,709 + -1.8 365,710 - +1.6 365,710 - +0.1 365,710 - +0.1 365,710 - +1.4 365,710 - +0.6 365,710 - -0.4 365,710 - -1.7 365,710 - +0.7 365,710 - -0.8 365,710 - -2.5 365,710 - -0.7 365,710 - -0.0 365,710 - +1.6 365,711 + +0.3 365,711 + +0.1 365,712 - -0.5 365,776 + +1.4 365,777 - -2.2 365,779 - +0.1 365,787 + +1.7 365,802 + -2.1 365,802 + +1.1 365,802 + -1.3 365,803 - -1.1 365,803 - +1.8 365,803 - -2.4 365,803 - +0.9 365,803 - +0.3 365,803 - +0.5 365,825 + +0.1 365,836 + -0.6 365,842 + -0.3 365,849 - +0.8 365,852 - +1.8 365,868 + +0.0 365,868 + +0.3 365,875 - -0.6 365,993 + EX31_RS04205 0.20 -1.2 366,161 + EX31_RS04205 0.43 -0.6 366,161 + EX31_RS04205 0.43 -0.3 366,162 - EX31_RS04205 0.44 -0.5 366,162 - EX31_RS04205 0.44 +1.2 366,162 - EX31_RS04205 0.44 -0.1 366,162 - EX31_RS04205 0.44 -0.2 366,163 + EX31_RS04205 0.44 +0.8 366,164 - EX31_RS04205 0.44 +0.4 366,232 + EX31_RS04205 0.53 -0.9 366,259 - EX31_RS04205 0.57 +0.6 366,259 - EX31_RS04205 0.57 -2.4 366,259 - EX31_RS04205 0.57 -0.1 366,262 - EX31_RS04205 0.57 -0.1 366,279 + EX31_RS04205 0.60 -0.4 366,279 + EX31_RS04205 0.60 -1.9 366,280 - EX31_RS04205 0.60 -0.2 366,283 + EX31_RS04205 0.60 -0.0 366,283 + EX31_RS04205 0.60 +0.5 366,283 + EX31_RS04205 0.60 -0.9 366,283 + EX31_RS04205 0.60 -0.7 366,283 + EX31_RS04205 0.60 -2.4 366,283 + EX31_RS04205 0.60 -0.8 366,283 + EX31_RS04205 0.60 +2.0 366,283 + EX31_RS04205 0.60 -1.3 366,284 - EX31_RS04205 0.60 -1.8 366,284 - EX31_RS04205 0.60 +0.2 366,284 - EX31_RS04205 0.60 -2.2 366,284 - EX31_RS04205 0.60 +0.0 366,284 - EX31_RS04205 0.60 -1.6 366,356 + EX31_RS04205 0.70 -0.8 366,357 - EX31_RS04205 0.70 +2.3 366,357 - EX31_RS04205 0.70 +1.0 366,385 + EX31_RS04205 0.74 -0.2 366,394 + EX31_RS04205 0.75 +1.7 366,395 - EX31_RS04205 0.75 -0.0 366,431 + EX31_RS04205 0.80 +1.6 366,431 + EX31_RS04205 0.80 -2.4 366,487 + EX31_RS04205 0.88 -0.1 366,491 + EX31_RS04205 0.89 -0.1 366,491 + EX31_RS04205 0.89 +1.1 366,492 - EX31_RS04205 0.89 -0.6 366,492 - EX31_RS04205 0.89 -0.8 366,532 + -1.0 366,532 + +0.2 366,532 + +0.4 366,542 + -0.5 366,568 + +0.2 366,568 + +1.1 366,568 + +0.4 366,569 - +0.1 366,726 + -0.9 366,727 - -2.3 366,727 - -0.1 366,727 - +1.1 366,889 - EX31_RS04210 0.15 +3.1 366,889 - EX31_RS04210 0.15 -0.2 366,889 - EX31_RS04210 0.15 -0.4 366,955 + EX31_RS04210 0.19 +0.1 366,955 + EX31_RS04210 0.19 +0.1 366,956 - EX31_RS04210 0.19 -0.7 366,956 - EX31_RS04210 0.19 -1.2 366,956 - EX31_RS04210 0.19 -0.2 367,054 + EX31_RS04210 0.25 -2.5 367,054 + EX31_RS04210 0.25 -0.2 367,055 - EX31_RS04210 0.25 +0.2 367,250 - EX31_RS04210 0.37 +0.3 367,391 - EX31_RS04210 0.45 +0.4 367,573 + EX31_RS04210 0.56 -1.1 367,574 - EX31_RS04210 0.56 +0.2 367,633 + EX31_RS04210 0.60 +0.1 367,633 + EX31_RS04210 0.60 -1.0 367,633 + EX31_RS04210 0.60 -0.3 367,634 - EX31_RS04210 0.60 +0.3 367,638 - EX31_RS04210 0.60 -0.5 367,638 - EX31_RS04210 0.60 +0.3 367,729 + EX31_RS04210 0.66 -0.4 367,730 - EX31_RS04210 0.66 -0.1 367,786 + EX31_RS04210 0.69 +2.4 367,816 + EX31_RS04210 0.71 +0.8 367,816 + EX31_RS04210 0.71 -0.2 367,816 + EX31_RS04210 0.71 +0.3 367,816 + EX31_RS04210 0.71 +0.1 367,816 + EX31_RS04210 0.71 +0.8 367,816 + EX31_RS04210 0.71 -2.1 367,816 + EX31_RS04210 0.71 -1.6 367,816 + EX31_RS04210 0.71 -0.8 367,817 - EX31_RS04210 0.71 +1.0 367,817 - EX31_RS04210 0.71 +0.4 367,817 - EX31_RS04210 0.71 -0.1 367,903 + EX31_RS04210 0.76 +0.1 367,903 + EX31_RS04210 0.76 +0.1 367,903 + EX31_RS04210 0.76 -1.6 367,904 - EX31_RS04210 0.76 -0.5 367,905 + EX31_RS04210 0.76 +0.4 367,905 + EX31_RS04210 0.76 -1.1 367,905 + EX31_RS04210 0.76 +0.9 367,906 - EX31_RS04210 0.77 -1.1 367,906 - EX31_RS04210 0.77 -1.5 367,906 - EX31_RS04210 0.77 +0.7 367,906 - EX31_RS04210 0.77 +0.1 367,910 + EX31_RS04210 0.77 +1.4 367,910 + EX31_RS04210 0.77 +1.2 367,911 - EX31_RS04210 0.77 -1.5 367,962 + EX31_RS04210 0.80 +1.3 367,963 - EX31_RS04210 0.80 +0.5 368,153 - +0.4 368,245 + +0.8 368,245 + -0.6 368,246 - -0.9 368,246 - -0.2 368,254 + -0.5 368,278 + -0.2 368,287 + -0.4 368,288 - -1.1 368,310 + +2.1 368,340 + -1.1 368,341 - +0.3 368,341 - +1.2 368,341 - +1.1 368,341 - +0.5 368,349 + -1.5 368,349 + -0.9 368,349 + +1.4 368,349 + +0.2 368,349 + -0.2 368,349 + -1.1 368,349 + -0.5 368,349 + +0.7 368,349 + +0.2 368,349 + -0.1 368,349 + -0.1 368,349 + -0.1 368,349 + -0.1 368,349 + -0.8 368,349 + -1.2 368,349 + -0.2 368,349 + +0.4 368,349 + +0.1 368,349 + -0.8 368,349 + -0.5 368,349 + +0.2 368,349 + +1.7 368,349 + +0.0 368,349 + -0.9 368,349 + +0.2 368,349 + -1.0 368,349 + -0.0 368,349 + -1.1 368,349 + -1.5 368,349 + -1.7 368,350 - +0.4 368,350 - -1.4 368,350 - +0.6 368,350 - -1.5 368,350 - +1.1 368,350 - -1.7 368,350 - -0.2 368,350 - +0.8 368,350 - -1.1 368,350 - -0.6 368,350 - +0.8 368,350 - -0.6 368,350 - +0.3 368,350 - +0.3 368,350 - +0.3 368,350 - +0.1 368,350 - +0.2 368,350 - +0.0 368,350 - +0.0 368,350 - -1.4 368,350 - +1.1 368,350 - -0.4 368,350 - +0.1 368,350 - -1.9 368,353 + -0.8 368,354 - -0.9 368,354 - -0.9 368,365 + +0.9 368,365 + -0.5 368,365 + +0.5 368,366 - -0.5 368,380 + +0.3 368,380 + -0.0 368,381 - -0.1 368,416 + -0.4 368,520 + +0.2 368,520 + -0.6 368,520 + +0.8 368,521 - +0.1 368,521 - +0.7 368,539 + +1.5 368,573 + +0.3 368,573 + +1.1 368,574 - -1.4 368,581 + EX31_RS04215 0.10 +0.5 368,708 - EX31_RS04215 0.20 +0.9 368,761 + EX31_RS04215 0.24 -0.0 368,761 + EX31_RS04215 0.24 +0.0 368,762 - EX31_RS04215 0.24 -0.9 368,762 - EX31_RS04215 0.24 +1.1 368,762 - EX31_RS04215 0.24 +0.4 368,836 + EX31_RS04215 0.29 -0.9 368,837 - EX31_RS04215 0.29 -0.5 368,907 + EX31_RS04215 0.34 +1.9 368,907 + EX31_RS04215 0.34 -0.5 368,907 + EX31_RS04215 0.34 -0.9 368,907 + EX31_RS04215 0.34 -1.2 368,908 - EX31_RS04215 0.34 +0.5 368,908 - EX31_RS04215 0.34 +0.0 368,908 - EX31_RS04215 0.34 +0.3 368,908 - EX31_RS04215 0.34 -0.4 368,908 - EX31_RS04215 0.34 +1.1 368,908 - EX31_RS04215 0.34 -0.6 368,910 + EX31_RS04215 0.35 -0.7 368,910 + EX31_RS04215 0.35 +0.6 368,911 - EX31_RS04215 0.35 -1.2 368,911 - EX31_RS04215 0.35 -0.2 368,911 - EX31_RS04215 0.35 +3.0 368,911 - EX31_RS04215 0.35 -1.5 368,911 - EX31_RS04215 0.35 +0.1 369,006 + EX31_RS04215 0.42 +0.3 369,006 + EX31_RS04215 0.42 +1.1 369,006 + EX31_RS04215 0.42 +0.3 369,006 + EX31_RS04215 0.42 -2.0 369,006 + EX31_RS04215 0.42 -0.9 369,007 - EX31_RS04215 0.42 +0.4 369,007 - EX31_RS04215 0.42 +0.4 369,262 + EX31_RS04215 0.60 +0.8 369,262 + EX31_RS04215 0.60 +0.2
Or see this region's nucleotide sequence