Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00615 and EX31_RS24915 are separated by 112 nucleotides EX31_RS24915 and EX31_RS00625 are separated by 378 nucleotides EX31_RS00625 and EX31_RS00630 are separated by 250 nucleotides EX31_RS00630 and EX31_RS00635 are separated by 95 nucleotides EX31_RS00635 and EX31_RS25035 overlap by 32 nucleotides
EX31_RS00615: EX31_RS00615 - heme anaerobic degradation radical SAM methyltransferase ChuW/HutW, at 128,413 to 129,738
_RS00615
EX31_RS24915: EX31_RS24915 - hypothetical protein, at 129,851 to 130,225
_RS24915
EX31_RS00625: EX31_RS00625 - YgdI/YgdR family lipoprotein, at 130,604 to 130,822
_RS00625
EX31_RS00630: EX31_RS00630 - iron dicitrate ABC transporter ATP-binding protein, at 131,073 to 131,306
_RS00630
EX31_RS00635: EX31_RS00635 - hypothetical protein, at 131,402 to 131,620
_RS00635
EX31_RS25035: EX31_RS25035 - hypothetical protein, at 131,589 to 131,855
_RS25035
Position (kb) 130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 129.714 kb on - strand at 129.714 kb on - strand at 129.731 kb on - strand at 129.739 kb on - strand at 129.811 kb on + strand at 129.835 kb on - strand at 129.872 kb on - strand at 129.872 kb on - strand at 129.872 kb on - strand at 129.904 kb on - strand, within EX31_RS24915 at 129.922 kb on + strand, within EX31_RS24915 at 129.922 kb on + strand, within EX31_RS24915 at 129.922 kb on + strand, within EX31_RS24915 at 129.923 kb on - strand, within EX31_RS24915 at 129.923 kb on - strand, within EX31_RS24915 at 129.923 kb on - strand, within EX31_RS24915 at 129.923 kb on - strand, within EX31_RS24915 at 129.923 kb on - strand, within EX31_RS24915 at 129.929 kb on - strand, within EX31_RS24915 at 129.929 kb on - strand, within EX31_RS24915 at 129.929 kb on - strand, within EX31_RS24915 at 129.929 kb on - strand, within EX31_RS24915 at 129.965 kb on - strand, within EX31_RS24915 at 129.965 kb on - strand, within EX31_RS24915 at 129.975 kb on + strand, within EX31_RS24915 at 129.975 kb on + strand, within EX31_RS24915 at 129.976 kb on - strand, within EX31_RS24915 at 129.980 kb on - strand, within EX31_RS24915 at 130.000 kb on + strand, within EX31_RS24915 at 130.000 kb on + strand, within EX31_RS24915 at 130.001 kb on - strand, within EX31_RS24915 at 130.005 kb on - strand, within EX31_RS24915 at 130.022 kb on + strand, within EX31_RS24915 at 130.023 kb on - strand, within EX31_RS24915 at 130.117 kb on + strand, within EX31_RS24915 at 130.182 kb on - strand, within EX31_RS24915 at 130.201 kb on + strand at 130.202 kb on - strand at 130.202 kb on - strand at 130.284 kb on + strand at 130.284 kb on + strand at 130.285 kb on - strand at 130.286 kb on + strand at 130.287 kb on - strand at 130.377 kb on + strand at 130.383 kb on - strand at 130.419 kb on + strand at 130.475 kb on + strand at 130.509 kb on + strand at 130.509 kb on + strand at 130.509 kb on + strand at 130.542 kb on + strand at 130.543 kb on - strand at 130.617 kb on + strand at 130.623 kb on + strand at 130.673 kb on + strand, within EX31_RS00625 at 130.673 kb on + strand, within EX31_RS00625 at 130.706 kb on - strand, within EX31_RS00625 at 130.847 kb on + strand at 130.854 kb on - strand at 130.857 kb on - strand at 130.882 kb on + strand at 130.882 kb on + strand at 130.999 kb on - strand at 131.064 kb on + strand at 131.077 kb on + strand at 131.077 kb on + strand at 131.078 kb on - strand at 131.078 kb on - strand at 131.157 kb on + strand, within EX31_RS00630 at 131.227 kb on + strand, within EX31_RS00630 at 131.227 kb on + strand, within EX31_RS00630 at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.294 kb on + strand at 131.295 kb on - strand at 131.320 kb on - strand at 131.363 kb on + strand at 131.376 kb on + strand at 131.376 kb on + strand at 131.377 kb on - strand at 131.388 kb on - strand at 131.501 kb on - strand, within EX31_RS00635 at 131.544 kb on + strand, within EX31_RS00635 at 131.564 kb on + strand, within EX31_RS00635 at 131.575 kb on - strand, within EX31_RS00635 at 131.626 kb on + strand, within EX31_RS25035 at 131.643 kb on + strand, within EX31_RS25035 at 131.687 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.697 kb on + strand, within EX31_RS25035 at 131.698 kb on - strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.699 kb on + strand, within EX31_RS25035 at 131.771 kb on + strand, within EX31_RS25035 at 131.802 kb on + strand, within EX31_RS25035
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 129,714 - +0.9 129,714 - -1.1 129,731 - +0.7 129,739 - +0.0 129,811 + -0.4 129,835 - -0.5 129,872 - -0.5 129,872 - -0.7 129,872 - +1.2 129,904 - EX31_RS24915 0.14 +0.9 129,922 + EX31_RS24915 0.19 +1.7 129,922 + EX31_RS24915 0.19 +1.9 129,922 + EX31_RS24915 0.19 -1.1 129,923 - EX31_RS24915 0.19 -0.1 129,923 - EX31_RS24915 0.19 -0.1 129,923 - EX31_RS24915 0.19 -0.1 129,923 - EX31_RS24915 0.19 -1.4 129,923 - EX31_RS24915 0.19 +0.7 129,929 - EX31_RS24915 0.21 -0.5 129,929 - EX31_RS24915 0.21 +1.4 129,929 - EX31_RS24915 0.21 -0.7 129,929 - EX31_RS24915 0.21 -1.6 129,965 - EX31_RS24915 0.30 +1.7 129,965 - EX31_RS24915 0.30 +3.0 129,975 + EX31_RS24915 0.33 +0.3 129,975 + EX31_RS24915 0.33 -1.0 129,976 - EX31_RS24915 0.33 +0.9 129,980 - EX31_RS24915 0.34 +0.0 130,000 + EX31_RS24915 0.40 -0.7 130,000 + EX31_RS24915 0.40 +0.9 130,001 - EX31_RS24915 0.40 +0.1 130,005 - EX31_RS24915 0.41 -1.3 130,022 + EX31_RS24915 0.46 -0.7 130,023 - EX31_RS24915 0.46 +0.2 130,117 + EX31_RS24915 0.71 +0.9 130,182 - EX31_RS24915 0.88 +0.1 130,201 + +1.2 130,202 - +0.0 130,202 - +0.2 130,284 + -0.6 130,284 + +2.4 130,285 - +0.2 130,286 + +0.0 130,287 - +0.2 130,377 + +1.4 130,383 - +0.5 130,419 + -0.9 130,475 + +1.0 130,509 + -1.7 130,509 + +0.0 130,509 + +0.6 130,542 + +0.9 130,543 - +2.9 130,617 + -0.9 130,623 + -2.1 130,673 + EX31_RS00625 0.32 -2.1 130,673 + EX31_RS00625 0.32 -1.7 130,706 - EX31_RS00625 0.47 +2.4 130,847 + -0.3 130,854 - -2.1 130,857 - +0.9 130,882 + +0.3 130,882 + +0.3 130,999 - +0.1 131,064 + -0.7 131,077 + -1.0 131,077 + -0.3 131,078 - -0.1 131,078 - +2.0 131,157 + EX31_RS00630 0.36 +0.3 131,227 + EX31_RS00630 0.66 +0.9 131,227 + EX31_RS00630 0.66 -1.7 131,294 + +0.4 131,294 + -0.7 131,294 + -1.6 131,294 + +0.3 131,294 + -0.7 131,295 - -0.7 131,320 - +1.8 131,363 + -0.1 131,376 + +2.3 131,376 + +0.0 131,377 - -0.7 131,388 - -0.1 131,501 - EX31_RS00635 0.45 -2.8 131,544 + EX31_RS00635 0.65 -0.9 131,564 + EX31_RS00635 0.74 +1.1 131,575 - EX31_RS00635 0.79 +2.9 131,626 + EX31_RS25035 0.14 -0.7 131,643 + EX31_RS25035 0.20 +0.4 131,687 + EX31_RS25035 0.37 -0.2 131,697 + EX31_RS25035 0.40 -0.1 131,697 + EX31_RS25035 0.40 +0.5 131,697 + EX31_RS25035 0.40 +0.4 131,697 + EX31_RS25035 0.40 -0.1 131,697 + EX31_RS25035 0.40 -0.7 131,698 - EX31_RS25035 0.41 -0.1 131,699 + EX31_RS25035 0.41 +1.4 131,699 + EX31_RS25035 0.41 -0.7 131,699 + EX31_RS25035 0.41 +0.4 131,699 + EX31_RS25035 0.41 +1.7 131,699 + EX31_RS25035 0.41 -0.1 131,699 + EX31_RS25035 0.41 +0.9 131,771 + EX31_RS25035 0.68 -0.7 131,802 + EX31_RS25035 0.80 +2.3
Or see this region's nucleotide sequence