Strain Fitness in Rahnella sp. WP5 around EX31_RS00010

Experiment: D-Mannitol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS25870 and EX31_RS00010 are separated by 67 nucleotidesEX31_RS00010 and EX31_RS00015 are separated by 4 nucleotidesEX31_RS00015 and EX31_RS00020 are separated by 187 nucleotides EX31_RS25870: EX31_RS25870 - hypothetical protein, at 381 to 524 _RS25870 EX31_RS00010: EX31_RS00010 - type II toxin-antitoxin system HipA family toxin, at 592 to 813 _RS00010 EX31_RS00015: EX31_RS00015 - helix-turn-helix transcriptional regulator, at 818 to 1,114 _RS00015 EX31_RS00020: EX31_RS00020 - GntR family transcriptional regulator, at 1,302 to 2,012 _RS00020 Position (kb) 0 1Strain fitness (log2 ratio) -2 -1 0 1 2at 0.037 kb on - strandat 0.070 kb on + strandat 0.093 kb on + strandat 0.203 kb on - strandat 0.238 kb on + strandat 0.292 kb on - strandat 0.325 kb on + strandat 0.383 kb on - strandat 0.531 kb on + strandat 0.542 kb on + strandat 0.543 kb on - strandat 0.663 kb on + strand, within EX31_RS00010at 0.723 kb on - strand, within EX31_RS00010at 0.727 kb on + strand, within EX31_RS00010at 0.771 kb on + strand, within EX31_RS00010at 0.785 kb on - strand, within EX31_RS00010at 0.813 kb on + strandat 0.814 kb on - strandat 0.814 kb on - strandat 0.845 kb on - strandat 0.845 kb on - strandat 0.849 kb on - strand, within EX31_RS00015at 0.874 kb on + strand, within EX31_RS00015at 0.874 kb on + strand, within EX31_RS00015at 0.874 kb on + strand, within EX31_RS00015at 0.874 kb on + strand, within EX31_RS00015at 0.875 kb on - strand, within EX31_RS00015at 0.875 kb on - strand, within EX31_RS00015at 0.931 kb on + strand, within EX31_RS00015at 0.931 kb on + strand, within EX31_RS00015at 0.931 kb on + strand, within EX31_RS00015at 0.932 kb on - strand, within EX31_RS00015at 0.932 kb on - strand, within EX31_RS00015at 0.932 kb on - strand, within EX31_RS00015at 0.965 kb on - strand, within EX31_RS00015at 0.990 kb on + strand, within EX31_RS00015at 0.991 kb on - strand, within EX31_RS00015at 0.991 kb on - strand, within EX31_RS00015at 0.991 kb on - strand, within EX31_RS00015at 0.991 kb on - strand, within EX31_RS00015at 0.991 kb on - strand, within EX31_RS00015at 1.085 kb on - strandat 1.085 kb on - strandat 1.085 kb on - strandat 1.101 kb on + strandat 1.102 kb on - strandat 1.102 kb on - strandat 1.115 kb on + strandat 1.130 kb on - strandat 1.130 kb on - strandat 1.142 kb on - strandat 1.150 kb on + strandat 1.150 kb on + strandat 1.159 kb on + strandat 1.159 kb on + strandat 1.160 kb on - strandat 1.172 kb on - strandat 1.319 kb on + strandat 1.319 kb on + strandat 1.494 kb on + strand, within EX31_RS00020at 1.494 kb on + strand, within EX31_RS00020at 1.494 kb on + strand, within EX31_RS00020at 1.647 kb on - strand, within EX31_RS00020at 1.690 kb on + strand, within EX31_RS00020at 1.690 kb on + strand, within EX31_RS00020at 1.770 kb on + strand, within EX31_RS00020at 1.770 kb on + strand, within EX31_RS00020at 1.771 kb on - strand, within EX31_RS00020

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannitol (C)
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37 - +0.4
70 + -0.4
93 + +0.3
203 - +0.0
238 + -0.5
292 - +2.4
325 + +1.1
383 - -1.0
531 + +0.4
542 + -0.1
543 - +0.1
663 + EX31_RS00010 0.32 +2.4
723 - EX31_RS00010 0.59 -0.5
727 + EX31_RS00010 0.61 -2.6
771 + EX31_RS00010 0.81 -1.5
785 - EX31_RS00010 0.87 -1.1
813 + -1.1
814 - -0.1
814 - +0.4
845 - -0.1
845 - +0.3
849 - EX31_RS00015 0.10 +0.9
874 + EX31_RS00015 0.19 +1.4
874 + EX31_RS00015 0.19 +2.4
874 + EX31_RS00015 0.19 -0.4
874 + EX31_RS00015 0.19 -2.1
875 - EX31_RS00015 0.19 +1.6
875 - EX31_RS00015 0.19 +2.2
931 + EX31_RS00015 0.38 -1.1
931 + EX31_RS00015 0.38 +1.2
931 + EX31_RS00015 0.38 +1.4
932 - EX31_RS00015 0.38 +0.9
932 - EX31_RS00015 0.38 +2.0
932 - EX31_RS00015 0.38 +0.5
965 - EX31_RS00015 0.49 +0.3
990 + EX31_RS00015 0.58 -0.1
991 - EX31_RS00015 0.58 -0.0
991 - EX31_RS00015 0.58 -0.7
991 - EX31_RS00015 0.58 -0.6
991 - EX31_RS00015 0.58 -1.0
991 - EX31_RS00015 0.58 -1.0
1,085 - -0.2
1,085 - +0.1
1,085 - -0.3
1,101 + -0.7
1,102 - -0.4
1,102 - -0.8
1,115 + +0.2
1,130 - -1.7
1,130 - +0.9
1,142 - +0.9
1,150 + -0.7
1,150 + -0.5
1,159 + +0.9
1,159 + +0.3
1,160 - -0.5
1,172 - -1.5
1,319 + +0.0
1,319 + -1.1
1,494 + EX31_RS00020 0.27 +1.9
1,494 + EX31_RS00020 0.27 -1.3
1,494 + EX31_RS00020 0.27 -0.3
1,647 - EX31_RS00020 0.49 +0.1
1,690 + EX31_RS00020 0.55 +0.0
1,690 + EX31_RS00020 0.55 +2.4
1,770 + EX31_RS00020 0.66 -0.7
1,770 + EX31_RS00020 0.66 -1.6
1,771 - EX31_RS00020 0.66 -0.3

Or see this region's nucleotide sequence