Experiment: CDM1 without Biotin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15555 and BBR_RS15560 are separated by 157 nucleotides BBR_RS15560 and BBR_RS15565 are separated by 500 nucleotides
BBR_RS15555: BBR_RS15555 - HAD family hydrolase, at 1,262,892 to 1,263,743
_RS15555
BBR_RS15560: BBR_RS15560 - aminotransferase class V-fold PLP-dependent enzyme, at 1,263,901 to 1,265,397
_RS15560
BBR_RS15565: BBR_RS15565 - hypothetical protein, at 1,265,898 to 1,267,082
_RS15565
Position (kb)
1263
1264
1265
1266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1262.902 kb on - strand at 1262.963 kb on - strand at 1263.007 kb on - strand, within BBR_RS15555 at 1263.135 kb on - strand, within BBR_RS15555 at 1263.140 kb on - strand, within BBR_RS15555 at 1263.187 kb on + strand, within BBR_RS15555 at 1263.195 kb on - strand, within BBR_RS15555 at 1263.241 kb on + strand, within BBR_RS15555 at 1263.515 kb on + strand, within BBR_RS15555 at 1263.653 kb on - strand, within BBR_RS15555 at 1263.663 kb on - strand at 1263.683 kb on + strand at 1263.891 kb on + strand at 1263.913 kb on + strand at 1263.941 kb on + strand at 1264.141 kb on + strand, within BBR_RS15560 at 1264.208 kb on - strand, within BBR_RS15560 at 1264.259 kb on + strand, within BBR_RS15560 at 1264.259 kb on + strand, within BBR_RS15560 at 1264.264 kb on + strand, within BBR_RS15560 at 1264.489 kb on + strand, within BBR_RS15560 at 1264.500 kb on + strand, within BBR_RS15560 at 1264.509 kb on + strand, within BBR_RS15560 at 1264.560 kb on + strand, within BBR_RS15560 at 1264.604 kb on + strand, within BBR_RS15560 at 1264.869 kb on + strand, within BBR_RS15560 at 1264.883 kb on + strand, within BBR_RS15560 at 1264.891 kb on - strand, within BBR_RS15560 at 1264.891 kb on - strand, within BBR_RS15560 at 1264.965 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.055 kb on + strand, within BBR_RS15560 at 1265.072 kb on + strand, within BBR_RS15560 at 1265.078 kb on - strand, within BBR_RS15560 at 1265.123 kb on - strand, within BBR_RS15560 at 1265.340 kb on + strand at 1265.340 kb on + strand at 1265.431 kb on + strand at 1265.473 kb on + strand at 1265.473 kb on + strand at 1265.531 kb on - strand at 1265.534 kb on + strand at 1265.546 kb on + strand at 1265.659 kb on - strand at 1265.807 kb on - strand at 1265.812 kb on + strand at 1265.815 kb on - strand at 1265.821 kb on + strand at 1265.896 kb on + strand at 1265.896 kb on + strand at 1265.927 kb on - strand at 1265.927 kb on - strand at 1265.939 kb on + strand at 1265.944 kb on + strand at 1265.944 kb on + strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.971 kb on + strand at 1266.024 kb on + strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.231 kb on - strand, within BBR_RS15565 at 1266.291 kb on - strand, within BBR_RS15565 at 1266.338 kb on - strand, within BBR_RS15565 at 1266.376 kb on + strand, within BBR_RS15565
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Biotin remove 1,262,902 - -0.2 1,262,963 - +0.8 1,263,007 - BBR_RS15555 0.13 -2.1 1,263,135 - BBR_RS15555 0.29 -1.0 1,263,140 - BBR_RS15555 0.29 +2.1 1,263,187 + BBR_RS15555 0.35 -1.3 1,263,195 - BBR_RS15555 0.36 -0.8 1,263,241 + BBR_RS15555 0.41 -1.1 1,263,515 + BBR_RS15555 0.73 -0.5 1,263,653 - BBR_RS15555 0.89 -2.7 1,263,663 - -0.9 1,263,683 + +1.4 1,263,891 + +1.0 1,263,913 + +0.7 1,263,941 + +0.1 1,264,141 + BBR_RS15560 0.16 +0.7 1,264,208 - BBR_RS15560 0.21 -2.4 1,264,259 + BBR_RS15560 0.24 +1.2 1,264,259 + BBR_RS15560 0.24 +0.9 1,264,264 + BBR_RS15560 0.24 -0.4 1,264,489 + BBR_RS15560 0.39 +0.3 1,264,500 + BBR_RS15560 0.40 +1.5 1,264,509 + BBR_RS15560 0.41 +1.3 1,264,560 + BBR_RS15560 0.44 +1.1 1,264,604 + BBR_RS15560 0.47 +0.6 1,264,869 + BBR_RS15560 0.65 +1.1 1,264,883 + BBR_RS15560 0.66 -0.3 1,264,891 - BBR_RS15560 0.66 -1.9 1,264,891 - BBR_RS15560 0.66 -1.0 1,264,965 + BBR_RS15560 0.71 +1.8 1,265,009 + BBR_RS15560 0.74 +1.1 1,265,009 + BBR_RS15560 0.74 +1.0 1,265,055 + BBR_RS15560 0.77 +0.3 1,265,072 + BBR_RS15560 0.78 +0.9 1,265,078 - BBR_RS15560 0.79 -2.1 1,265,123 - BBR_RS15560 0.82 -1.1 1,265,340 + +1.2 1,265,340 + +0.3 1,265,431 + -1.3 1,265,473 + +0.1 1,265,473 + -0.1 1,265,531 - +0.4 1,265,534 + -1.0 1,265,546 + -0.4 1,265,659 - +0.5 1,265,807 - -3.0 1,265,812 + -1.0 1,265,815 - -0.9 1,265,821 + -1.0 1,265,896 + -2.5 1,265,896 + -2.3 1,265,927 - +0.2 1,265,927 - +0.6 1,265,939 + +0.2 1,265,944 + +0.6 1,265,944 + +0.2 1,265,955 - +1.0 1,265,955 - -0.2 1,265,955 - +0.4 1,265,955 - +0.3 1,265,971 + +2.6 1,266,024 + BBR_RS15565 0.11 +0.9 1,266,043 - BBR_RS15565 0.12 +0.2 1,266,043 - BBR_RS15565 0.12 -0.9 1,266,110 + BBR_RS15565 0.18 -0.4 1,266,110 + BBR_RS15565 0.18 -0.1 1,266,145 - BBR_RS15565 0.21 +0.2 1,266,145 - BBR_RS15565 0.21 +2.0 1,266,178 + BBR_RS15565 0.24 +0.4 1,266,178 + BBR_RS15565 0.24 -0.5 1,266,231 - BBR_RS15565 0.28 +0.9 1,266,291 - BBR_RS15565 0.33 -0.2 1,266,338 - BBR_RS15565 0.37 -0.4 1,266,376 + BBR_RS15565 0.40 -0.1
Or see this region's nucleotide sequence