Experiment: CDM1 without Biotin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13020 and BBR_RS13025 are separated by 351 nucleotides BBR_RS13025 and BBR_RS13030 are separated by 2 nucleotides
BBR_RS13020: BBR_RS13020 - DNA polymerase III subunit epsilon, at 693,711 to 695,165
_RS13020
BBR_RS13025: BBR_RS13025 - peptide chain release factor 2, at 695,517 to 696,641
_RS13025
BBR_RS13030: BBR_RS13030 - cell division ATP-binding protein FtsE, at 696,644 to 697,840
_RS13030
Position (kb)
695
696
697 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 694.577 kb on - strand, within BBR_RS13020 at 694.600 kb on + strand, within BBR_RS13020 at 694.603 kb on - strand, within BBR_RS13020 at 694.618 kb on + strand, within BBR_RS13020 at 694.626 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020 at 694.760 kb on + strand, within BBR_RS13020 at 694.765 kb on + strand, within BBR_RS13020 at 694.794 kb on - strand, within BBR_RS13020 at 694.796 kb on + strand, within BBR_RS13020 at 694.804 kb on - strand, within BBR_RS13020 at 694.819 kb on + strand, within BBR_RS13020 at 694.819 kb on - strand, within BBR_RS13020 at 694.827 kb on - strand, within BBR_RS13020 at 694.834 kb on + strand, within BBR_RS13020 at 694.892 kb on + strand, within BBR_RS13020 at 694.902 kb on - strand, within BBR_RS13020 at 694.904 kb on + strand, within BBR_RS13020 at 694.943 kb on - strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.952 kb on + strand, within BBR_RS13020 at 694.960 kb on - strand, within BBR_RS13020 at 694.969 kb on + strand, within BBR_RS13020 at 694.971 kb on + strand, within BBR_RS13020 at 694.977 kb on + strand, within BBR_RS13020 at 694.977 kb on - strand, within BBR_RS13020 at 694.978 kb on - strand, within BBR_RS13020 at 694.988 kb on + strand, within BBR_RS13020 at 694.988 kb on + strand, within BBR_RS13020 at 694.996 kb on - strand, within BBR_RS13020 at 694.996 kb on - strand, within BBR_RS13020 at 694.996 kb on - strand, within BBR_RS13020 at 695.026 kb on + strand at 695.094 kb on - strand at 695.113 kb on - strand at 695.156 kb on + strand at 695.156 kb on + strand at 695.166 kb on + strand at 695.171 kb on + strand at 695.191 kb on + strand at 695.201 kb on + strand at 695.201 kb on + strand at 695.201 kb on + strand at 695.201 kb on + strand at 695.266 kb on + strand at 695.279 kb on - strand at 695.393 kb on + strand at 695.404 kb on + strand at 695.428 kb on + strand at 695.458 kb on + strand at 696.612 kb on - strand at 696.792 kb on - strand, within BBR_RS13030 at 696.853 kb on - strand, within BBR_RS13030 at 696.885 kb on + strand, within BBR_RS13030 at 697.152 kb on + strand, within BBR_RS13030 at 697.165 kb on - strand, within BBR_RS13030 at 697.436 kb on + strand, within BBR_RS13030 at 697.539 kb on - strand, within BBR_RS13030 at 697.610 kb on + strand, within BBR_RS13030 at 697.618 kb on + strand, within BBR_RS13030
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Biotin remove 694,577 - BBR_RS13020 0.60 -2.6 694,600 + BBR_RS13020 0.61 +0.9 694,603 - BBR_RS13020 0.61 -0.2 694,618 + BBR_RS13020 0.62 -0.4 694,626 - BBR_RS13020 0.63 -0.4 694,653 - BBR_RS13020 0.65 -0.6 694,653 - BBR_RS13020 0.65 +0.1 694,653 - BBR_RS13020 0.65 -1.6 694,760 + BBR_RS13020 0.72 +0.0 694,765 + BBR_RS13020 0.72 -0.6 694,794 - BBR_RS13020 0.74 -1.0 694,796 + BBR_RS13020 0.75 -0.4 694,804 - BBR_RS13020 0.75 +0.3 694,819 + BBR_RS13020 0.76 -0.0 694,819 - BBR_RS13020 0.76 -0.0 694,827 - BBR_RS13020 0.77 -1.8 694,834 + BBR_RS13020 0.77 -0.0 694,892 + BBR_RS13020 0.81 +1.7 694,902 - BBR_RS13020 0.82 +0.3 694,904 + BBR_RS13020 0.82 +0.0 694,943 - BBR_RS13020 0.85 -0.7 694,952 + BBR_RS13020 0.85 +0.5 694,952 + BBR_RS13020 0.85 -2.2 694,952 + BBR_RS13020 0.85 +0.3 694,952 + BBR_RS13020 0.85 -0.4 694,952 + BBR_RS13020 0.85 -0.8 694,952 + BBR_RS13020 0.85 +0.1 694,952 + BBR_RS13020 0.85 -0.1 694,960 - BBR_RS13020 0.86 -1.0 694,969 + BBR_RS13020 0.86 +0.7 694,971 + BBR_RS13020 0.87 +0.9 694,977 + BBR_RS13020 0.87 +1.3 694,977 - BBR_RS13020 0.87 -0.8 694,978 - BBR_RS13020 0.87 -0.5 694,988 + BBR_RS13020 0.88 -0.7 694,988 + BBR_RS13020 0.88 -0.9 694,996 - BBR_RS13020 0.88 -0.1 694,996 - BBR_RS13020 0.88 +0.4 694,996 - BBR_RS13020 0.88 +0.7 695,026 + +1.2 695,094 - +0.5 695,113 - -1.2 695,156 + +0.2 695,156 + +0.9 695,166 + -0.5 695,171 + -1.5 695,191 + -1.4 695,201 + -0.7 695,201 + +0.1 695,201 + +0.2 695,201 + -0.5 695,266 + +0.5 695,279 - -0.3 695,393 + +0.2 695,404 + +0.6 695,428 + -1.9 695,458 + -0.4 696,612 - -1.9 696,792 - BBR_RS13030 0.12 -3.8 696,853 - BBR_RS13030 0.17 -3.9 696,885 + BBR_RS13030 0.20 -3.1 697,152 + BBR_RS13030 0.42 -2.2 697,165 - BBR_RS13030 0.44 -4.0 697,436 + BBR_RS13030 0.66 -1.9 697,539 - BBR_RS13030 0.75 -4.2 697,610 + BBR_RS13030 0.81 -3.5 697,618 + BBR_RS13030 0.81 -2.9
Or see this region's nucleotide sequence