Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22685 and EX31_RS22690 are separated by 220 nucleotides EX31_RS22690 and EX31_RS22695 are separated by 36 nucleotides EX31_RS22695 and EX31_RS22700 are separated by 232 nucleotides EX31_RS22700 and EX31_RS22705 are separated by 81 nucleotides
EX31_RS22685: EX31_RS22685 - spermidine N1-acetyltransferase, at 297,412 to 297,987
_RS22685
EX31_RS22690: EX31_RS22690 - phosphoribosylglycinamide formyltransferase, at 298,208 to 298,846
_RS22690
EX31_RS22695: EX31_RS22695 - phosphoribosylformylglycinamidine cyclo-ligase, at 298,883 to 299,920
_RS22695
EX31_RS22700: EX31_RS22700 - uracil phosphoribosyltransferase, at 300,153 to 300,779
_RS22700
EX31_RS22705: EX31_RS22705 - uracil permease, at 300,861 to 302,153
_RS22705
Position (kb) 298
299
300 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 297.912 kb on + strand, within EX31_RS22685 at 297.913 kb on - strand, within EX31_RS22685 at 297.915 kb on + strand, within EX31_RS22685 at 297.915 kb on + strand, within EX31_RS22685 at 297.916 kb on - strand, within EX31_RS22685 at 297.916 kb on - strand, within EX31_RS22685 at 297.961 kb on + strand at 297.961 kb on + strand at 297.962 kb on - strand at 297.975 kb on + strand at 297.975 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.003 kb on + strand at 298.004 kb on - strand at 298.004 kb on - strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.005 kb on + strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.006 kb on - strand at 298.034 kb on + strand at 298.034 kb on + strand at 298.035 kb on - strand at 298.038 kb on + strand at 298.038 kb on + strand at 298.038 kb on + strand at 298.039 kb on - strand at 298.092 kb on + strand at 298.093 kb on - strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.115 kb on + strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.116 kb on - strand at 298.181 kb on - strand at 298.181 kb on - strand at 298.183 kb on - strand at 298.183 kb on - strand at 298.204 kb on + strand at 298.210 kb on - strand at 298.337 kb on + strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.338 kb on - strand, within EX31_RS22690 at 298.500 kb on + strand, within EX31_RS22690 at 298.573 kb on + strand, within EX31_RS22690 at 298.573 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.579 kb on + strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.580 kb on - strand, within EX31_RS22690 at 298.581 kb on + strand, within EX31_RS22690 at 298.582 kb on - strand, within EX31_RS22690 at 298.582 kb on - strand, within EX31_RS22690 at 298.592 kb on + strand, within EX31_RS22690 at 298.592 kb on + strand, within EX31_RS22690 at 298.593 kb on - strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.669 kb on + strand, within EX31_RS22690 at 298.670 kb on - strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.722 kb on + strand, within EX31_RS22690 at 298.735 kb on + strand, within EX31_RS22690 at 298.735 kb on + strand, within EX31_RS22690 at 298.736 kb on - strand, within EX31_RS22690 at 298.803 kb on - strand at 298.803 kb on - strand at 298.803 kb on - strand at 298.824 kb on - strand at 298.876 kb on - strand at 298.921 kb on + strand at 298.921 kb on + strand at 298.921 kb on + strand at 298.922 kb on - strand at 298.954 kb on + strand at 299.026 kb on + strand, within EX31_RS22695 at 299.026 kb on + strand, within EX31_RS22695 at 299.027 kb on - strand, within EX31_RS22695 at 299.027 kb on - strand, within EX31_RS22695 at 299.038 kb on - strand, within EX31_RS22695 at 299.038 kb on - strand, within EX31_RS22695 at 299.038 kb on - strand, within EX31_RS22695 at 299.038 kb on - strand, within EX31_RS22695 at 299.038 kb on - strand, within EX31_RS22695 at 299.141 kb on + strand, within EX31_RS22695 at 299.141 kb on + strand, within EX31_RS22695 at 299.141 kb on + strand, within EX31_RS22695 at 299.142 kb on - strand, within EX31_RS22695 at 299.340 kb on + strand, within EX31_RS22695 at 299.475 kb on + strand, within EX31_RS22695 at 299.475 kb on + strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.530 kb on + strand, within EX31_RS22695 at 299.530 kb on + strand, within EX31_RS22695 at 299.595 kb on + strand, within EX31_RS22695 at 299.596 kb on - strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.674 kb on + strand, within EX31_RS22695 at 299.675 kb on - strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.678 kb on - strand, within EX31_RS22695 at 299.831 kb on + strand at 299.832 kb on - strand at 299.834 kb on + strand at 299.834 kb on + strand at 299.834 kb on + strand at 299.835 kb on - strand at 299.835 kb on - strand at 299.835 kb on - strand at 299.852 kb on + strand at 299.852 kb on + strand at 299.853 kb on - strand at 299.853 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.977 kb on + strand at 299.978 kb on - strand at 299.978 kb on - strand at 300.008 kb on + strand at 300.025 kb on + strand at 300.026 kb on - strand at 300.034 kb on + strand at 300.034 kb on + strand at 300.034 kb on + strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.037 kb on - strand at 300.051 kb on - strand at 300.072 kb on - strand at 300.072 kb on - strand at 300.104 kb on + strand at 300.104 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.140 kb on - strand at 300.148 kb on - strand at 300.151 kb on + strand at 300.151 kb on + strand at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.274 kb on - strand, within EX31_RS22700 at 300.274 kb on - strand, within EX31_RS22700 at 300.292 kb on + strand, within EX31_RS22700 at 300.292 kb on + strand, within EX31_RS22700 at 300.293 kb on - strand, within EX31_RS22700 at 300.351 kb on - strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.371 kb on - strand, within EX31_RS22700 at 300.378 kb on - strand, within EX31_RS22700 at 300.413 kb on - strand, within EX31_RS22700 at 300.437 kb on + strand, within EX31_RS22700 at 300.490 kb on - strand, within EX31_RS22700 at 300.490 kb on - strand, within EX31_RS22700 at 300.510 kb on - strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.567 kb on - strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.687 kb on + strand, within EX31_RS22700 at 300.687 kb on + strand, within EX31_RS22700 at 300.688 kb on - strand, within EX31_RS22700 at 300.719 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.727 kb on - strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.771 kb on - strand at 300.777 kb on + strand at 300.777 kb on + strand at 300.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 297,912 + EX31_RS22685 0.87 -1.5 297,913 - EX31_RS22685 0.87 +0.4 297,915 + EX31_RS22685 0.87 -0.4 297,915 + EX31_RS22685 0.87 +0.1 297,916 - EX31_RS22685 0.88 -0.6 297,916 - EX31_RS22685 0.88 -0.1 297,961 + -0.4 297,961 + -0.3 297,962 - +0.1 297,975 + -0.1 297,975 + -0.3 298,003 + -0.9 298,003 + +0.6 298,003 + +0.7 298,003 + -0.1 298,004 - -0.6 298,004 - +1.0 298,005 + -0.1 298,005 + +0.8 298,005 + -0.2 298,005 + +0.1 298,005 + -0.7 298,005 + +1.1 298,005 + -0.4 298,006 - +0.9 298,006 - -0.1 298,006 - -0.3 298,006 - +0.0 298,006 - +1.7 298,006 - +0.6 298,006 - -0.5 298,006 - +0.3 298,034 + -1.7 298,034 + -2.0 298,035 - -0.3 298,038 + +1.1 298,038 + -0.9 298,038 + +0.3 298,039 - +0.7 298,092 + +0.9 298,093 - +1.5 298,115 + +0.7 298,115 + +1.1 298,115 + -0.3 298,115 + +2.9 298,116 - -0.4 298,116 - -0.1 298,116 - +1.3 298,116 - -1.5 298,116 - -0.1 298,181 - -1.9 298,181 - -0.4 298,183 - -0.6 298,183 - -0.4 298,204 + -0.7 298,210 - -0.9 298,337 + EX31_RS22690 0.20 -0.4 298,338 - EX31_RS22690 0.20 -0.4 298,338 - EX31_RS22690 0.20 -1.6 298,338 - EX31_RS22690 0.20 -1.1 298,338 - EX31_RS22690 0.20 -2.1 298,338 - EX31_RS22690 0.20 -1.1 298,500 + EX31_RS22690 0.46 -2.1 298,573 + EX31_RS22690 0.57 -1.4 298,573 + EX31_RS22690 0.57 -3.0 298,579 + EX31_RS22690 0.58 -1.9 298,579 + EX31_RS22690 0.58 -0.9 298,579 + EX31_RS22690 0.58 +0.6 298,579 + EX31_RS22690 0.58 -0.1 298,579 + EX31_RS22690 0.58 -1.7 298,579 + EX31_RS22690 0.58 -2.9 298,579 + EX31_RS22690 0.58 -0.1 298,579 + EX31_RS22690 0.58 +0.9 298,579 + EX31_RS22690 0.58 -2.3 298,579 + EX31_RS22690 0.58 -0.7 298,579 + EX31_RS22690 0.58 -1.1 298,579 + EX31_RS22690 0.58 -0.9 298,579 + EX31_RS22690 0.58 -2.6 298,579 + EX31_RS22690 0.58 -2.6 298,579 + EX31_RS22690 0.58 -0.7 298,579 + EX31_RS22690 0.58 +0.9 298,580 - EX31_RS22690 0.58 -1.7 298,580 - EX31_RS22690 0.58 -1.2 298,580 - EX31_RS22690 0.58 -2.7 298,580 - EX31_RS22690 0.58 -1.0 298,580 - EX31_RS22690 0.58 -2.4 298,580 - EX31_RS22690 0.58 -2.1 298,580 - EX31_RS22690 0.58 -3.0 298,580 - EX31_RS22690 0.58 -2.9 298,580 - EX31_RS22690 0.58 -3.0 298,580 - EX31_RS22690 0.58 -0.7 298,580 - EX31_RS22690 0.58 -0.1 298,580 - EX31_RS22690 0.58 -0.6 298,580 - EX31_RS22690 0.58 -2.4 298,580 - EX31_RS22690 0.58 -1.9 298,580 - EX31_RS22690 0.58 -0.7 298,581 + EX31_RS22690 0.58 -0.1 298,582 - EX31_RS22690 0.59 -1.1 298,582 - EX31_RS22690 0.59 -0.4 298,592 + EX31_RS22690 0.60 -1.4 298,592 + EX31_RS22690 0.60 +0.3 298,593 - EX31_RS22690 0.60 -1.4 298,669 + EX31_RS22690 0.72 -2.4 298,669 + EX31_RS22690 0.72 -0.8 298,669 + EX31_RS22690 0.72 -2.7 298,670 - EX31_RS22690 0.72 +0.0 298,722 + EX31_RS22690 0.80 +0.2 298,722 + EX31_RS22690 0.80 -0.1 298,722 + EX31_RS22690 0.80 +0.9 298,735 + EX31_RS22690 0.82 -2.6 298,735 + EX31_RS22690 0.82 -1.7 298,736 - EX31_RS22690 0.83 -2.2 298,803 - -2.1 298,803 - -2.4 298,803 - -1.9 298,824 - -2.9 298,876 - -0.8 298,921 + -0.7 298,921 + -1.3 298,921 + -0.1 298,922 - +0.5 298,954 + -0.5 299,026 + EX31_RS22695 0.14 -0.1 299,026 + EX31_RS22695 0.14 -2.4 299,027 - EX31_RS22695 0.14 -2.4 299,027 - EX31_RS22695 0.14 -2.4 299,038 - EX31_RS22695 0.15 -2.9 299,038 - EX31_RS22695 0.15 -3.3 299,038 - EX31_RS22695 0.15 -2.9 299,038 - EX31_RS22695 0.15 -1.7 299,038 - EX31_RS22695 0.15 -3.7 299,141 + EX31_RS22695 0.25 -0.7 299,141 + EX31_RS22695 0.25 -2.1 299,141 + EX31_RS22695 0.25 -2.3 299,142 - EX31_RS22695 0.25 -3.2 299,340 + EX31_RS22695 0.44 -2.7 299,475 + EX31_RS22695 0.57 -1.8 299,475 + EX31_RS22695 0.57 -1.1 299,476 - EX31_RS22695 0.57 -3.1 299,476 - EX31_RS22695 0.57 -2.4 299,476 - EX31_RS22695 0.57 -3.5 299,476 - EX31_RS22695 0.57 -3.4 299,530 + EX31_RS22695 0.62 -1.7 299,530 + EX31_RS22695 0.62 -3.8 299,595 + EX31_RS22695 0.69 -1.4 299,596 - EX31_RS22695 0.69 -2.9 299,598 + EX31_RS22695 0.69 -1.9 299,598 + EX31_RS22695 0.69 -1.7 299,598 + EX31_RS22695 0.69 -2.9 299,674 + EX31_RS22695 0.76 -0.9 299,675 - EX31_RS22695 0.76 -2.9 299,677 + EX31_RS22695 0.76 -0.7 299,677 + EX31_RS22695 0.76 +0.9 299,677 + EX31_RS22695 0.76 -1.9 299,678 - EX31_RS22695 0.77 -2.8 299,831 + -2.7 299,832 - -2.4 299,834 + -2.3 299,834 + -2.8 299,834 + -0.7 299,835 - -2.7 299,835 - -3.1 299,835 - -3.9 299,852 + -0.1 299,852 + -3.0 299,853 - -1.7 299,853 - +0.9 299,880 - -3.5 299,880 - -2.9 299,880 - -4.0 299,880 - -1.4 299,880 - -2.2 299,880 - -2.6 299,895 + -3.9 299,895 + -3.8 299,895 + -3.5 299,895 + -2.3 299,896 - -2.6 299,896 - -3.1 299,896 - -2.6 299,896 - -3.7 299,977 + -1.1 299,978 - +0.9 299,978 - -0.4 300,008 + +0.9 300,025 + -0.2 300,026 - +0.9 300,034 + +0.1 300,034 + +0.3 300,034 + -1.2 300,035 - -0.2 300,035 - +0.9 300,035 - +0.1 300,035 - +1.9 300,037 - +1.0 300,051 - +1.4 300,072 - +1.5 300,072 - +0.9 300,104 + -1.3 300,104 + -1.3 300,139 + -0.8 300,139 + -0.4 300,139 + +1.1 300,139 + -0.8 300,139 + +0.1 300,139 + -2.2 300,139 + +2.7 300,140 - +1.9 300,148 - +0.6 300,151 + +0.4 300,151 + -1.1 300,273 + EX31_RS22700 0.19 +0.3 300,273 + EX31_RS22700 0.19 +1.0 300,273 + EX31_RS22700 0.19 +0.5 300,273 + EX31_RS22700 0.19 -0.1 300,274 - EX31_RS22700 0.19 -0.1 300,274 - EX31_RS22700 0.19 -0.7 300,292 + EX31_RS22700 0.22 +0.5 300,292 + EX31_RS22700 0.22 -1.5 300,293 - EX31_RS22700 0.22 -0.3 300,351 - EX31_RS22700 0.32 -0.5 300,370 + EX31_RS22700 0.35 -0.1 300,370 + EX31_RS22700 0.35 +1.1 300,370 + EX31_RS22700 0.35 -1.8 300,371 - EX31_RS22700 0.35 +0.9 300,378 - EX31_RS22700 0.36 +0.5 300,413 - EX31_RS22700 0.41 +2.5 300,437 + EX31_RS22700 0.45 -1.1 300,490 - EX31_RS22700 0.54 -1.4 300,490 - EX31_RS22700 0.54 +1.9 300,510 - EX31_RS22700 0.57 -0.1 300,566 + EX31_RS22700 0.66 -0.9 300,566 + EX31_RS22700 0.66 -1.1 300,566 + EX31_RS22700 0.66 -0.6 300,566 + EX31_RS22700 0.66 -0.6 300,567 - EX31_RS22700 0.66 -1.2 300,614 + EX31_RS22700 0.74 +1.4 300,614 + EX31_RS22700 0.74 +0.4 300,614 + EX31_RS22700 0.74 -0.2 300,687 + EX31_RS22700 0.85 -0.1 300,687 + EX31_RS22700 0.85 -0.7 300,688 - EX31_RS22700 0.85 -1.1 300,719 + -3.0 300,726 + +0.7 300,726 + +0.5 300,726 + -1.7 300,726 + +0.7 300,726 + +1.9 300,726 + +0.7 300,726 + +0.7 300,726 + -0.7 300,726 + -3.4 300,727 - -0.4 300,763 + +1.6 300,763 + -0.1 300,763 + +1.1 300,763 + +0.1 300,763 + +1.4 300,763 + +1.2 300,771 - +1.5 300,777 + -0.9 300,777 + -0.2 300,777 + +1.1
Or see this region's nucleotide sequence