Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21415 and EX31_RS21420 are separated by 136 nucleotides EX31_RS21420 and EX31_RS21425 are separated by 134 nucleotides
EX31_RS21415: EX31_RS21415 - class II fructose-bisphosphate aldolase, at 23,329 to 24,408
_RS21415
EX31_RS21420: EX31_RS21420 - LacI family DNA-binding transcriptional regulator, at 24,545 to 25,627
_RS21420
EX31_RS21425: EX31_RS21425 - beta-galactosidase, at 25,762 to 28,860
_RS21425
Position (kb) 24
25
26 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 24.401 kb on + strand at 24.401 kb on + strand at 24.401 kb on + strand at 24.401 kb on + strand at 24.401 kb on + strand at 24.402 kb on - strand at 24.402 kb on - strand at 24.406 kb on + strand at 24.406 kb on + strand at 24.406 kb on + strand at 24.406 kb on + strand at 24.406 kb on + strand at 24.406 kb on + strand at 24.407 kb on - strand at 24.414 kb on - strand at 24.510 kb on - strand at 24.599 kb on + strand at 24.689 kb on + strand, within EX31_RS21420 at 24.734 kb on + strand, within EX31_RS21420 at 24.735 kb on - strand, within EX31_RS21420 at 24.768 kb on + strand, within EX31_RS21420 at 24.768 kb on + strand, within EX31_RS21420 at 24.769 kb on - strand, within EX31_RS21420 at 24.769 kb on - strand, within EX31_RS21420 at 24.820 kb on - strand, within EX31_RS21420 at 25.126 kb on - strand, within EX31_RS21420 at 25.137 kb on + strand, within EX31_RS21420 at 25.138 kb on - strand, within EX31_RS21420 at 25.138 kb on - strand, within EX31_RS21420 at 25.138 kb on - strand, within EX31_RS21420 at 25.142 kb on + strand, within EX31_RS21420 at 25.143 kb on - strand, within EX31_RS21420 at 25.180 kb on + strand, within EX31_RS21420 at 25.180 kb on + strand, within EX31_RS21420 at 25.180 kb on + strand, within EX31_RS21420 at 25.240 kb on - strand, within EX31_RS21420 at 25.309 kb on - strand, within EX31_RS21420 at 25.376 kb on - strand, within EX31_RS21420 at 25.464 kb on + strand, within EX31_RS21420 at 25.478 kb on - strand, within EX31_RS21420 at 25.510 kb on - strand, within EX31_RS21420 at 25.510 kb on - strand, within EX31_RS21420 at 25.510 kb on - strand, within EX31_RS21420 at 25.582 kb on - strand at 25.694 kb on + strand at 25.695 kb on - strand at 25.695 kb on - strand at 25.695 kb on - strand at 25.695 kb on - strand at 25.695 kb on - strand at 25.698 kb on + strand at 25.699 kb on - strand at 25.759 kb on - strand at 25.762 kb on - strand at 25.788 kb on - strand at 25.960 kb on + strand at 26.074 kb on + strand, within EX31_RS21425 at 26.077 kb on - strand, within EX31_RS21425 at 26.227 kb on + strand, within EX31_RS21425 at 26.227 kb on + strand, within EX31_RS21425 at 26.227 kb on + strand, within EX31_RS21425 at 26.228 kb on - strand, within EX31_RS21425 at 26.228 kb on - strand, within EX31_RS21425 at 26.231 kb on + strand, within EX31_RS21425 at 26.231 kb on + strand, within EX31_RS21425 at 26.231 kb on + strand, within EX31_RS21425 at 26.231 kb on + strand, within EX31_RS21425 at 26.232 kb on - strand, within EX31_RS21425 at 26.363 kb on - strand, within EX31_RS21425 at 26.379 kb on + strand, within EX31_RS21425 at 26.379 kb on + strand, within EX31_RS21425 at 26.380 kb on - strand, within EX31_RS21425 at 26.380 kb on - strand, within EX31_RS21425 at 26.380 kb on - strand, within EX31_RS21425 at 26.425 kb on - strand, within EX31_RS21425 at 26.425 kb on - strand, within EX31_RS21425 at 26.501 kb on + strand, within EX31_RS21425 at 26.502 kb on - strand, within EX31_RS21425 at 26.502 kb on - strand, within EX31_RS21425 at 26.502 kb on - strand, within EX31_RS21425 at 26.542 kb on + strand, within EX31_RS21425 at 26.542 kb on + strand, within EX31_RS21425 at 26.543 kb on - strand, within EX31_RS21425 at 26.561 kb on + strand, within EX31_RS21425
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 24,401 + -1.7 24,401 + -0.6 24,401 + -0.1 24,401 + +0.6 24,401 + -1.2 24,402 - +0.9 24,402 - +1.5 24,406 + -1.3 24,406 + +1.9 24,406 + -0.1 24,406 + -0.8 24,406 + -2.5 24,406 + -0.8 24,407 - -0.1 24,414 - -0.8 24,510 - +0.0 24,599 + +0.9 24,689 + EX31_RS21420 0.13 +0.9 24,734 + EX31_RS21420 0.17 -2.1 24,735 - EX31_RS21420 0.18 +0.4 24,768 + EX31_RS21420 0.21 +0.8 24,768 + EX31_RS21420 0.21 +0.3 24,769 - EX31_RS21420 0.21 +0.5 24,769 - EX31_RS21420 0.21 -0.1 24,820 - EX31_RS21420 0.25 -1.7 25,126 - EX31_RS21420 0.54 -0.7 25,137 + EX31_RS21420 0.55 +1.1 25,138 - EX31_RS21420 0.55 -0.3 25,138 - EX31_RS21420 0.55 +0.1 25,138 - EX31_RS21420 0.55 -1.4 25,142 + EX31_RS21420 0.55 +1.6 25,143 - EX31_RS21420 0.55 -0.6 25,180 + EX31_RS21420 0.59 -0.0 25,180 + EX31_RS21420 0.59 -0.2 25,180 + EX31_RS21420 0.59 -0.5 25,240 - EX31_RS21420 0.64 +0.7 25,309 - EX31_RS21420 0.71 -1.1 25,376 - EX31_RS21420 0.77 +0.6 25,464 + EX31_RS21420 0.85 +0.2 25,478 - EX31_RS21420 0.86 -0.3 25,510 - EX31_RS21420 0.89 +0.2 25,510 - EX31_RS21420 0.89 -1.1 25,510 - EX31_RS21420 0.89 +1.5 25,582 - -0.8 25,694 + +0.6 25,695 - -0.3 25,695 - -0.3 25,695 - -0.2 25,695 - -0.8 25,695 - +1.3 25,698 + -0.7 25,699 - +0.3 25,759 - +0.2 25,762 - +2.0 25,788 - -0.5 25,960 + -0.5 26,074 + EX31_RS21425 0.10 +0.9 26,077 - EX31_RS21425 0.10 -0.3 26,227 + EX31_RS21425 0.15 +0.7 26,227 + EX31_RS21425 0.15 +0.1 26,227 + EX31_RS21425 0.15 -0.5 26,228 - EX31_RS21425 0.15 -0.9 26,228 - EX31_RS21425 0.15 +0.6 26,231 + EX31_RS21425 0.15 -0.0 26,231 + EX31_RS21425 0.15 +0.2 26,231 + EX31_RS21425 0.15 -0.0 26,231 + EX31_RS21425 0.15 +2.3 26,232 - EX31_RS21425 0.15 -0.4 26,363 - EX31_RS21425 0.19 -1.7 26,379 + EX31_RS21425 0.20 +0.1 26,379 + EX31_RS21425 0.20 -0.8 26,380 - EX31_RS21425 0.20 -0.4 26,380 - EX31_RS21425 0.20 -0.1 26,380 - EX31_RS21425 0.20 -0.6 26,425 - EX31_RS21425 0.21 -1.7 26,425 - EX31_RS21425 0.21 +0.8 26,501 + EX31_RS21425 0.24 -0.8 26,502 - EX31_RS21425 0.24 -3.0 26,502 - EX31_RS21425 0.24 +0.5 26,502 - EX31_RS21425 0.24 +0.9 26,542 + EX31_RS21425 0.25 +0.9 26,542 + EX31_RS21425 0.25 -0.8 26,543 - EX31_RS21425 0.25 -0.4 26,561 + EX31_RS21425 0.26 +2.3
Or see this region's nucleotide sequence