Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11555 and EX31_RS11560 are separated by 144 nucleotides EX31_RS11560 and EX31_RS11565 are separated by 168 nucleotides EX31_RS11565 and EX31_RS11570 are separated by 25 nucleotides
EX31_RS11555: EX31_RS11555 - methionine--tRNA ligase, at 249,784 to 251,814
_RS11555
EX31_RS11560: EX31_RS11560 - DUF1127 domain-containing protein, at 251,959 to 252,231
_RS11560
EX31_RS11565: EX31_RS11565 - PLP-dependent aminotransferase family protein, at 252,400 to 253,818
_RS11565
EX31_RS11570: EX31_RS11570 - LysR family transcriptional regulator, at 253,844 to 254,734
_RS11570
Position (kb)
252
253
254 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 251.447 kb on + strand, within EX31_RS11555 at 251.915 kb on - strand at 251.956 kb on + strand at 252.093 kb on + strand, within EX31_RS11560 at 252.093 kb on + strand, within EX31_RS11560 at 252.093 kb on + strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.094 kb on - strand, within EX31_RS11560 at 252.171 kb on + strand, within EX31_RS11560 at 252.223 kb on - strand at 252.223 kb on - strand at 252.268 kb on - strand at 252.441 kb on + strand at 252.458 kb on - strand at 252.494 kb on + strand at 252.660 kb on + strand, within EX31_RS11565 at 252.661 kb on - strand, within EX31_RS11565 at 252.693 kb on + strand, within EX31_RS11565 at 252.693 kb on + strand, within EX31_RS11565 at 252.694 kb on - strand, within EX31_RS11565 at 252.851 kb on + strand, within EX31_RS11565 at 252.894 kb on + strand, within EX31_RS11565 at 252.895 kb on - strand, within EX31_RS11565 at 253.131 kb on - strand, within EX31_RS11565 at 253.166 kb on - strand, within EX31_RS11565 at 253.166 kb on - strand, within EX31_RS11565 at 253.186 kb on - strand, within EX31_RS11565 at 253.246 kb on + strand, within EX31_RS11565 at 253.262 kb on - strand, within EX31_RS11565 at 253.287 kb on + strand, within EX31_RS11565 at 253.287 kb on + strand, within EX31_RS11565 at 253.287 kb on + strand, within EX31_RS11565 at 253.287 kb on + strand, within EX31_RS11565 at 253.288 kb on - strand, within EX31_RS11565 at 253.288 kb on - strand, within EX31_RS11565 at 253.288 kb on - strand, within EX31_RS11565 at 253.313 kb on + strand, within EX31_RS11565 at 253.411 kb on + strand, within EX31_RS11565 at 253.411 kb on + strand, within EX31_RS11565 at 253.411 kb on + strand, within EX31_RS11565 at 253.411 kb on + strand, within EX31_RS11565 at 253.412 kb on - strand, within EX31_RS11565 at 253.412 kb on - strand, within EX31_RS11565 at 253.412 kb on - strand, within EX31_RS11565 at 253.412 kb on - strand, within EX31_RS11565 at 253.480 kb on + strand, within EX31_RS11565 at 253.480 kb on + strand, within EX31_RS11565 at 253.480 kb on + strand, within EX31_RS11565 at 253.502 kb on + strand, within EX31_RS11565 at 253.513 kb on + strand, within EX31_RS11565 at 253.555 kb on + strand, within EX31_RS11565 at 253.667 kb on + strand, within EX31_RS11565 at 253.667 kb on + strand, within EX31_RS11565 at 253.667 kb on + strand, within EX31_RS11565 at 253.735 kb on + strand at 253.735 kb on + strand at 253.735 kb on + strand at 253.735 kb on + strand at 253.735 kb on + strand at 253.735 kb on + strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.841 kb on - strand at 253.852 kb on - strand at 253.852 kb on - strand at 253.852 kb on - strand at 253.997 kb on - strand, within EX31_RS11570 at 254.021 kb on - strand, within EX31_RS11570 at 254.052 kb on - strand, within EX31_RS11570 at 254.068 kb on + strand, within EX31_RS11570 at 254.069 kb on - strand, within EX31_RS11570 at 254.084 kb on - strand, within EX31_RS11570 at 254.286 kb on - strand, within EX31_RS11570 at 254.317 kb on - strand, within EX31_RS11570 at 254.526 kb on - strand, within EX31_RS11570 at 254.526 kb on - strand, within EX31_RS11570 at 254.597 kb on + strand, within EX31_RS11570 at 254.597 kb on + strand, within EX31_RS11570 at 254.597 kb on + strand, within EX31_RS11570 at 254.597 kb on + strand, within EX31_RS11570 at 254.597 kb on + strand, within EX31_RS11570 at 254.660 kb on + strand at 254.661 kb on - strand at 254.661 kb on - strand at 254.661 kb on - strand at 254.704 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 251,447 + EX31_RS11555 0.82 -1.9 251,915 - +1.1 251,956 + +0.5 252,093 + EX31_RS11560 0.49 -0.3 252,093 + EX31_RS11560 0.49 -1.3 252,093 + EX31_RS11560 0.49 -0.1 252,094 - EX31_RS11560 0.49 -0.5 252,094 - EX31_RS11560 0.49 -0.5 252,094 - EX31_RS11560 0.49 +0.5 252,094 - EX31_RS11560 0.49 +0.7 252,094 - EX31_RS11560 0.49 -0.3 252,094 - EX31_RS11560 0.49 -1.6 252,171 + EX31_RS11560 0.78 -0.3 252,223 - +1.4 252,223 - +0.9 252,268 - -1.1 252,441 + -1.1 252,458 - +3.0 252,494 + +0.7 252,660 + EX31_RS11565 0.18 +1.5 252,661 - EX31_RS11565 0.18 +0.7 252,693 + EX31_RS11565 0.21 -2.2 252,693 + EX31_RS11565 0.21 +1.7 252,694 - EX31_RS11565 0.21 +1.4 252,851 + EX31_RS11565 0.32 -0.6 252,894 + EX31_RS11565 0.35 +0.3 252,895 - EX31_RS11565 0.35 -0.9 253,131 - EX31_RS11565 0.52 +0.9 253,166 - EX31_RS11565 0.54 -0.3 253,166 - EX31_RS11565 0.54 -0.6 253,186 - EX31_RS11565 0.55 +0.5 253,246 + EX31_RS11565 0.60 +0.2 253,262 - EX31_RS11565 0.61 +1.1 253,287 + EX31_RS11565 0.63 +0.7 253,287 + EX31_RS11565 0.63 +2.5 253,287 + EX31_RS11565 0.63 +1.9 253,287 + EX31_RS11565 0.63 +0.9 253,288 - EX31_RS11565 0.63 -1.3 253,288 - EX31_RS11565 0.63 +0.1 253,288 - EX31_RS11565 0.63 +0.6 253,313 + EX31_RS11565 0.64 +2.3 253,411 + EX31_RS11565 0.71 +1.1 253,411 + EX31_RS11565 0.71 +0.7 253,411 + EX31_RS11565 0.71 -0.9 253,411 + EX31_RS11565 0.71 +0.4 253,412 - EX31_RS11565 0.71 -0.1 253,412 - EX31_RS11565 0.71 +0.1 253,412 - EX31_RS11565 0.71 +0.3 253,412 - EX31_RS11565 0.71 -0.5 253,480 + EX31_RS11565 0.76 -1.9 253,480 + EX31_RS11565 0.76 +0.9 253,480 + EX31_RS11565 0.76 +0.1 253,502 + EX31_RS11565 0.78 -1.6 253,513 + EX31_RS11565 0.78 -0.6 253,555 + EX31_RS11565 0.81 -0.6 253,667 + EX31_RS11565 0.89 -0.3 253,667 + EX31_RS11565 0.89 -2.3 253,667 + EX31_RS11565 0.89 +0.9 253,735 + +1.0 253,735 + +0.4 253,735 + +0.5 253,735 + +0.7 253,735 + -0.3 253,735 + -1.0 253,736 - -0.1 253,736 - -0.3 253,841 - -0.5 253,852 - +0.3 253,852 - +0.7 253,852 - +0.1 253,997 - EX31_RS11570 0.17 +0.8 254,021 - EX31_RS11570 0.20 -0.3 254,052 - EX31_RS11570 0.23 +0.7 254,068 + EX31_RS11570 0.25 +0.1 254,069 - EX31_RS11570 0.25 -0.1 254,084 - EX31_RS11570 0.27 +1.1 254,286 - EX31_RS11570 0.50 -0.9 254,317 - EX31_RS11570 0.53 +0.1 254,526 - EX31_RS11570 0.77 +1.7 254,526 - EX31_RS11570 0.77 -1.6 254,597 + EX31_RS11570 0.85 -1.9 254,597 + EX31_RS11570 0.85 +1.4 254,597 + EX31_RS11570 0.85 +0.4 254,597 + EX31_RS11570 0.85 -0.9 254,597 + EX31_RS11570 0.85 +0.7 254,660 + -0.3 254,661 - +1.1 254,661 - +0.5 254,661 - +0.1 254,704 + -2.1
Or see this region's nucleotide sequence