Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03000 and EX31_RS03005 overlap by 11 nucleotides EX31_RS03005 and EX31_RS03010 are separated by 130 nucleotides EX31_RS03010 and EX31_RS03015 are separated by 98 nucleotides EX31_RS03015 and EX31_RS03020 overlap by 4 nucleotides
EX31_RS03000: EX31_RS03000 - hypothetical protein, at 83,492 to 83,746
_RS03000
EX31_RS03005: EX31_RS03005 - hypothetical protein, at 83,736 to 84,392
_RS03005
EX31_RS03010: EX31_RS03010 - helix-turn-helix domain-containing protein, at 84,523 to 85,071
_RS03010
EX31_RS03015: EX31_RS03015 - branched-chain amino acid ABC transporter permease, at 85,170 to 85,838
_RS03015
EX31_RS03020: EX31_RS03020 - AzlD domain-containing protein, at 85,835 to 86,158
_RS03020
Position (kb) 84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 83.524 kb on + strand, within EX31_RS03000 at 83.536 kb on + strand, within EX31_RS03000 at 83.609 kb on - strand, within EX31_RS03000 at 83.663 kb on - strand, within EX31_RS03000 at 83.669 kb on + strand, within EX31_RS03000 at 83.670 kb on - strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.680 kb on + strand, within EX31_RS03000 at 83.681 kb on - strand, within EX31_RS03000 at 83.681 kb on - strand, within EX31_RS03000 at 83.684 kb on + strand, within EX31_RS03000 at 83.684 kb on + strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.685 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.690 kb on - strand, within EX31_RS03000 at 83.701 kb on - strand, within EX31_RS03000 at 83.701 kb on - strand, within EX31_RS03000 at 83.726 kb on - strand at 83.726 kb on - strand at 83.738 kb on - strand at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.814 kb on + strand, within EX31_RS03005 at 83.815 kb on - strand, within EX31_RS03005 at 83.835 kb on + strand, within EX31_RS03005 at 83.835 kb on + strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.836 kb on - strand, within EX31_RS03005 at 83.857 kb on + strand, within EX31_RS03005 at 83.859 kb on + strand, within EX31_RS03005 at 83.860 kb on - strand, within EX31_RS03005 at 83.870 kb on - strand, within EX31_RS03005 at 83.894 kb on - strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.958 kb on + strand, within EX31_RS03005 at 83.959 kb on - strand, within EX31_RS03005 at 83.959 kb on - strand, within EX31_RS03005 at 84.031 kb on - strand, within EX31_RS03005 at 84.033 kb on + strand, within EX31_RS03005 at 84.045 kb on + strand, within EX31_RS03005 at 84.045 kb on + strand, within EX31_RS03005 at 84.060 kb on + strand, within EX31_RS03005 at 84.060 kb on + strand, within EX31_RS03005 at 84.093 kb on - strand, within EX31_RS03005 at 84.179 kb on + strand, within EX31_RS03005 at 84.180 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.226 kb on - strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.243 kb on + strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.244 kb on - strand, within EX31_RS03005 at 84.247 kb on + strand, within EX31_RS03005 at 84.247 kb on + strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.248 kb on - strand, within EX31_RS03005 at 84.274 kb on + strand, within EX31_RS03005 at 84.274 kb on + strand, within EX31_RS03005 at 84.275 kb on - strand, within EX31_RS03005 at 84.275 kb on - strand, within EX31_RS03005 at 84.328 kb on + strand at 84.330 kb on + strand at 84.330 kb on + strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.331 kb on - strand at 84.351 kb on - strand at 84.409 kb on + strand at 84.413 kb on - strand at 84.431 kb on - strand at 84.439 kb on - strand at 84.439 kb on - strand at 84.442 kb on - strand at 84.462 kb on + strand at 84.463 kb on - strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.467 kb on + strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.468 kb on - strand at 84.469 kb on + strand at 84.469 kb on + strand at 84.469 kb on + strand at 84.469 kb on + strand at 84.469 kb on + strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.470 kb on - strand at 84.471 kb on + strand at 84.471 kb on + strand at 84.471 kb on + strand at 84.471 kb on + strand at 84.471 kb on + strand at 84.471 kb on + strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.472 kb on - strand at 84.486 kb on - strand at 84.511 kb on + strand at 84.579 kb on + strand, within EX31_RS03010 at 84.580 kb on - strand, within EX31_RS03010 at 84.580 kb on - strand, within EX31_RS03010 at 84.599 kb on + strand, within EX31_RS03010 at 84.608 kb on + strand, within EX31_RS03010 at 84.608 kb on + strand, within EX31_RS03010 at 84.609 kb on - strand, within EX31_RS03010 at 84.609 kb on - strand, within EX31_RS03010 at 84.746 kb on + strand, within EX31_RS03010 at 84.747 kb on - strand, within EX31_RS03010 at 84.747 kb on - strand, within EX31_RS03010 at 84.750 kb on + strand, within EX31_RS03010 at 84.751 kb on - strand, within EX31_RS03010 at 84.751 kb on - strand, within EX31_RS03010 at 84.751 kb on - strand, within EX31_RS03010 at 84.785 kb on - strand, within EX31_RS03010 at 84.788 kb on - strand, within EX31_RS03010 at 84.795 kb on + strand, within EX31_RS03010 at 84.796 kb on - strand, within EX31_RS03010 at 84.796 kb on - strand, within EX31_RS03010 at 84.796 kb on - strand, within EX31_RS03010 at 84.797 kb on + strand, within EX31_RS03010 at 84.798 kb on - strand, within EX31_RS03010 at 84.855 kb on + strand, within EX31_RS03010 at 84.890 kb on - strand, within EX31_RS03010 at 85.051 kb on - strand at 85.056 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.072 kb on - strand at 85.072 kb on - strand at 85.169 kb on + strand at 85.169 kb on + strand at 85.170 kb on - strand at 85.211 kb on + strand at 85.211 kb on + strand at 85.211 kb on + strand at 85.348 kb on + strand, within EX31_RS03015 at 85.348 kb on + strand, within EX31_RS03015 at 85.349 kb on - strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.426 kb on + strand, within EX31_RS03015 at 85.427 kb on - strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.533 kb on - strand, within EX31_RS03015 at 85.558 kb on - strand, within EX31_RS03015 at 85.585 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.618 kb on - strand, within EX31_RS03015 at 85.618 kb on - strand, within EX31_RS03015 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.981 kb on - strand, within EX31_RS03020 at 85.999 kb on + strand, within EX31_RS03020
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 83,524 + EX31_RS03000 0.13 +2.6 83,536 + EX31_RS03000 0.17 -0.1 83,609 - EX31_RS03000 0.46 +0.4 83,663 - EX31_RS03000 0.67 -1.8 83,669 + EX31_RS03000 0.69 +0.3 83,670 - EX31_RS03000 0.70 +0.2 83,680 + EX31_RS03000 0.74 -0.7 83,680 + EX31_RS03000 0.74 -0.5 83,680 + EX31_RS03000 0.74 -1.4 83,681 - EX31_RS03000 0.74 +1.6 83,681 - EX31_RS03000 0.74 -0.5 83,684 + EX31_RS03000 0.75 +0.1 83,684 + EX31_RS03000 0.75 -0.8 83,685 - EX31_RS03000 0.76 +1.4 83,685 - EX31_RS03000 0.76 -1.8 83,685 - EX31_RS03000 0.76 +0.1 83,685 - EX31_RS03000 0.76 +0.1 83,690 - EX31_RS03000 0.78 -2.2 83,690 - EX31_RS03000 0.78 -0.7 83,690 - EX31_RS03000 0.78 +0.2 83,690 - EX31_RS03000 0.78 +0.8 83,690 - EX31_RS03000 0.78 -0.2 83,701 - EX31_RS03000 0.82 +1.2 83,701 - EX31_RS03000 0.82 +0.3 83,726 - -0.9 83,726 - -0.5 83,738 - +0.3 83,814 + EX31_RS03005 0.12 -0.1 83,814 + EX31_RS03005 0.12 +0.1 83,814 + EX31_RS03005 0.12 -1.9 83,814 + EX31_RS03005 0.12 -0.1 83,814 + EX31_RS03005 0.12 -2.3 83,815 - EX31_RS03005 0.12 -0.1 83,835 + EX31_RS03005 0.15 -1.3 83,835 + EX31_RS03005 0.15 +1.2 83,836 - EX31_RS03005 0.15 +0.1 83,836 - EX31_RS03005 0.15 -0.9 83,836 - EX31_RS03005 0.15 -0.4 83,836 - EX31_RS03005 0.15 -1.0 83,836 - EX31_RS03005 0.15 +0.2 83,857 + EX31_RS03005 0.18 +0.1 83,859 + EX31_RS03005 0.19 -0.3 83,860 - EX31_RS03005 0.19 -0.8 83,870 - EX31_RS03005 0.20 -0.9 83,894 - EX31_RS03005 0.24 +1.1 83,958 + EX31_RS03005 0.34 -0.6 83,958 + EX31_RS03005 0.34 +1.7 83,958 + EX31_RS03005 0.34 +1.5 83,958 + EX31_RS03005 0.34 -1.3 83,959 - EX31_RS03005 0.34 +2.6 83,959 - EX31_RS03005 0.34 -0.5 84,031 - EX31_RS03005 0.45 +1.1 84,033 + EX31_RS03005 0.45 +0.5 84,045 + EX31_RS03005 0.47 +0.8 84,045 + EX31_RS03005 0.47 +0.1 84,060 + EX31_RS03005 0.49 +0.3 84,060 + EX31_RS03005 0.49 +0.1 84,093 - EX31_RS03005 0.54 +0.7 84,179 + EX31_RS03005 0.67 +2.3 84,180 - EX31_RS03005 0.68 +0.4 84,226 - EX31_RS03005 0.75 -0.9 84,226 - EX31_RS03005 0.75 -1.3 84,226 - EX31_RS03005 0.75 +0.1 84,243 + EX31_RS03005 0.77 +0.4 84,243 + EX31_RS03005 0.77 +1.1 84,243 + EX31_RS03005 0.77 +2.2 84,243 + EX31_RS03005 0.77 +0.9 84,243 + EX31_RS03005 0.77 +1.5 84,243 + EX31_RS03005 0.77 -0.1 84,243 + EX31_RS03005 0.77 -0.5 84,243 + EX31_RS03005 0.77 -0.5 84,244 - EX31_RS03005 0.77 +0.8 84,244 - EX31_RS03005 0.77 -1.8 84,244 - EX31_RS03005 0.77 +0.9 84,244 - EX31_RS03005 0.77 +1.1 84,244 - EX31_RS03005 0.77 -0.3 84,244 - EX31_RS03005 0.77 +1.1 84,244 - EX31_RS03005 0.77 +1.1 84,244 - EX31_RS03005 0.77 +1.6 84,244 - EX31_RS03005 0.77 +0.1 84,244 - EX31_RS03005 0.77 +2.4 84,247 + EX31_RS03005 0.78 -0.2 84,247 + EX31_RS03005 0.78 +1.1 84,248 - EX31_RS03005 0.78 -0.6 84,248 - EX31_RS03005 0.78 +0.6 84,248 - EX31_RS03005 0.78 +0.6 84,248 - EX31_RS03005 0.78 -1.3 84,248 - EX31_RS03005 0.78 +1.4 84,274 + EX31_RS03005 0.82 -1.3 84,274 + EX31_RS03005 0.82 -0.5 84,275 - EX31_RS03005 0.82 +0.1 84,275 - EX31_RS03005 0.82 +0.9 84,328 + +0.3 84,330 + +2.4 84,330 + -0.3 84,331 - -0.6 84,331 - +0.4 84,331 - +0.9 84,331 - -0.2 84,331 - -1.2 84,331 - -2.1 84,351 - -1.3 84,409 + +0.7 84,413 - -1.1 84,431 - -0.0 84,439 - +0.3 84,439 - +0.4 84,442 - +4.2 84,462 + -0.8 84,463 - -1.8 84,467 + +1.6 84,467 + -0.2 84,467 + -0.3 84,467 + +0.2 84,467 + -1.3 84,467 + -0.9 84,467 + -0.3 84,467 + -0.1 84,467 + +1.1 84,467 + +0.9 84,468 - -0.2 84,468 - +0.5 84,468 - +0.6 84,468 - +0.1 84,468 - +1.4 84,468 - +1.1 84,468 - +0.4 84,468 - +1.1 84,468 - -1.3 84,468 - +0.5 84,469 + -0.3 84,469 + +1.7 84,469 + +0.3 84,469 + +0.8 84,469 + +0.7 84,470 - -0.6 84,470 - +0.7 84,470 - -0.4 84,470 - -0.4 84,470 - +0.6 84,470 - -0.2 84,470 - -1.2 84,471 + +0.8 84,471 + -0.5 84,471 + +1.5 84,471 + +1.1 84,471 + +1.1 84,471 + +1.1 84,472 - +0.4 84,472 - -0.4 84,472 - +0.4 84,472 - -1.6 84,472 - +0.8 84,472 - +1.3 84,472 - +1.4 84,472 - +0.2 84,472 - -0.5 84,486 - +0.1 84,511 + +1.1 84,579 + EX31_RS03010 0.10 -1.5 84,580 - EX31_RS03010 0.10 +0.1 84,580 - EX31_RS03010 0.10 -0.9 84,599 + EX31_RS03010 0.14 -1.4 84,608 + EX31_RS03010 0.15 -0.5 84,608 + EX31_RS03010 0.15 -1.0 84,609 - EX31_RS03010 0.16 -1.1 84,609 - EX31_RS03010 0.16 +0.8 84,746 + EX31_RS03010 0.41 -1.5 84,747 - EX31_RS03010 0.41 +1.1 84,747 - EX31_RS03010 0.41 -0.7 84,750 + EX31_RS03010 0.41 -1.8 84,751 - EX31_RS03010 0.42 -0.8 84,751 - EX31_RS03010 0.42 -1.5 84,751 - EX31_RS03010 0.42 -0.9 84,785 - EX31_RS03010 0.48 -1.8 84,788 - EX31_RS03010 0.48 -1.5 84,795 + EX31_RS03010 0.50 -1.4 84,796 - EX31_RS03010 0.50 +1.1 84,796 - EX31_RS03010 0.50 -1.1 84,796 - EX31_RS03010 0.50 -2.3 84,797 + EX31_RS03010 0.50 -1.3 84,798 - EX31_RS03010 0.50 -3.6 84,855 + EX31_RS03010 0.60 -1.1 84,890 - EX31_RS03010 0.67 -3.3 85,051 - -0.9 85,056 + -0.5 85,059 + -0.5 85,059 + -1.3 85,059 + +2.1 85,059 + +0.1 85,059 + -1.3 85,059 + -2.1 85,059 + -0.5 85,059 + -1.5 85,059 + -0.3 85,059 + -3.5 85,059 + -1.0 85,059 + -1.8 85,059 + -1.9 85,059 + -2.2 85,059 + -1.5 85,059 + +0.6 85,060 - -2.6 85,060 - -0.9 85,060 - +0.1 85,060 - -1.2 85,060 - +0.6 85,060 - -1.1 85,060 - -2.1 85,060 - -2.5 85,060 - +0.5 85,060 - -0.4 85,060 - +0.1 85,060 - -2.1 85,060 - -1.3 85,060 - -2.1 85,060 - -1.1 85,060 - +0.1 85,060 - -0.9 85,060 - +1.1 85,071 + -2.1 85,071 + -0.8 85,071 + -0.6 85,071 + -2.1 85,072 - +0.5 85,072 - -0.5 85,169 + -0.8 85,169 + +1.1 85,170 - +2.1 85,211 + -0.2 85,211 + +0.2 85,211 + -2.0 85,348 + EX31_RS03015 0.27 +0.2 85,348 + EX31_RS03015 0.27 +1.1 85,349 - EX31_RS03015 0.27 -0.9 85,352 + EX31_RS03015 0.27 -0.7 85,352 + EX31_RS03015 0.27 +3.1 85,352 + EX31_RS03015 0.27 +1.6 85,426 + EX31_RS03015 0.38 +1.5 85,427 - EX31_RS03015 0.38 +0.6 85,532 + EX31_RS03015 0.54 -1.9 85,532 + EX31_RS03015 0.54 +1.5 85,532 + EX31_RS03015 0.54 +0.5 85,532 + EX31_RS03015 0.54 +1.1 85,533 - EX31_RS03015 0.54 +0.7 85,558 - EX31_RS03015 0.58 +0.4 85,585 + EX31_RS03015 0.62 -0.6 85,617 + EX31_RS03015 0.67 +0.6 85,617 + EX31_RS03015 0.67 +1.2 85,617 + EX31_RS03015 0.67 +0.1 85,617 + EX31_RS03015 0.67 +0.6 85,618 - EX31_RS03015 0.67 -1.5 85,618 - EX31_RS03015 0.67 +0.5 85,980 + EX31_RS03020 0.45 +0.1 85,980 + EX31_RS03020 0.45 -0.0 85,980 + EX31_RS03020 0.45 +1.9 85,980 + EX31_RS03020 0.45 +1.1 85,980 + EX31_RS03020 0.45 +2.2 85,981 - EX31_RS03020 0.45 +0.5 85,999 + EX31_RS03020 0.51 +0.3
Or see this region's nucleotide sequence