Strain Fitness in Rahnella sp. WP5 around EX31_RS01615

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS01600 and EX31_RS01605 are separated by 7 nucleotidesEX31_RS01605 and EX31_RS01610 overlap by 4 nucleotidesEX31_RS01610 and EX31_RS01615 are separated by 8 nucleotidesEX31_RS01615 and EX31_RS01620 overlap by 8 nucleotidesEX31_RS01620 and EX31_RS01625 are separated by 85 nucleotides EX31_RS01600: EX31_RS01600 - tail fiber protein, at 357,406 to 358,143 _RS01600 EX31_RS01605: EX31_RS01605 - tail protein, at 358,151 to 358,555 _RS01605 EX31_RS01610: EX31_RS01610 - phage tail protein, at 358,552 to 359,103 _RS01610 EX31_RS01615: EX31_RS01615 - DNA breaking-rejoining protein, at 359,112 to 359,387 _RS01615 EX31_RS01620: EX31_RS01620 - DUF2190 family protein, at 359,380 to 359,706 _RS01620 EX31_RS01625: EX31_RS01625 - Clp protease ClpP, at 359,792 to 361,750 _RS01625 Position (kb) 359 360Strain fitness (log2 ratio) -2 -1 0 1 2at 358.144 kb on - strandat 358.158 kb on - strandat 358.161 kb on + strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.162 kb on - strandat 358.174 kb on - strandat 358.222 kb on - strand, within EX31_RS01605at 358.227 kb on + strand, within EX31_RS01605at 358.227 kb on + strand, within EX31_RS01605at 358.228 kb on - strand, within EX31_RS01605at 358.350 kb on - strand, within EX31_RS01605at 358.423 kb on - strand, within EX31_RS01605at 358.550 kb on + strandat 358.550 kb on + strandat 358.560 kb on + strandat 358.646 kb on + strand, within EX31_RS01610at 358.646 kb on + strand, within EX31_RS01610at 358.647 kb on - strand, within EX31_RS01610at 358.657 kb on - strand, within EX31_RS01610at 358.774 kb on - strand, within EX31_RS01610at 359.058 kb on + strandat 359.103 kb on + strandat 359.103 kb on + strandat 359.103 kb on + strandat 359.168 kb on - strand, within EX31_RS01615at 359.168 kb on - strand, within EX31_RS01615at 359.168 kb on - strand, within EX31_RS01615at 359.183 kb on - strand, within EX31_RS01615at 359.183 kb on - strand, within EX31_RS01615at 359.491 kb on - strand, within EX31_RS01620at 359.493 kb on - strand, within EX31_RS01620at 359.493 kb on - strand, within EX31_RS01620at 359.621 kb on - strand, within EX31_RS01620at 359.621 kb on - strand, within EX31_RS01620at 359.665 kb on - strand, within EX31_RS01620at 359.743 kb on + strandat 359.744 kb on - strandat 359.744 kb on - strandat 359.747 kb on + strandat 359.794 kb on - strandat 359.904 kb on + strandat 359.904 kb on + strandat 359.905 kb on - strandat 359.905 kb on - strandat 359.928 kb on + strandat 360.268 kb on + strand, within EX31_RS01625at 360.306 kb on + strand, within EX31_RS01625at 360.306 kb on + strand, within EX31_RS01625at 360.307 kb on - strand, within EX31_RS01625

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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358,144 - +1.0
358,158 - +0.7
358,161 + +0.7
358,162 - -1.6
358,162 - -0.6
358,162 - +1.0
358,162 - +0.5
358,162 - -2.2
358,162 - -0.3
358,162 - -0.0
358,174 - +0.4
358,222 - EX31_RS01605 0.18 -0.0
358,227 + EX31_RS01605 0.19 +0.4
358,227 + EX31_RS01605 0.19 -0.8
358,228 - EX31_RS01605 0.19 +0.4
358,350 - EX31_RS01605 0.49 +0.2
358,423 - EX31_RS01605 0.67 +0.7
358,550 + +1.0
358,550 + -0.6
358,560 + +1.4
358,646 + EX31_RS01610 0.17 -0.0
358,646 + EX31_RS01610 0.17 +0.6
358,647 - EX31_RS01610 0.17 -0.0
358,657 - EX31_RS01610 0.19 +0.6
358,774 - EX31_RS01610 0.40 +0.4
359,058 + -1.5
359,103 + +0.5
359,103 + +1.0
359,103 + -0.0
359,168 - EX31_RS01615 0.20 +1.0
359,168 - EX31_RS01615 0.20 -2.0
359,168 - EX31_RS01615 0.20 +0.1
359,183 - EX31_RS01615 0.26 +1.0
359,183 - EX31_RS01615 0.26 -1.7
359,491 - EX31_RS01620 0.34 +0.2
359,493 - EX31_RS01620 0.35 -1.4
359,493 - EX31_RS01620 0.35 +0.2
359,621 - EX31_RS01620 0.74 -0.2
359,621 - EX31_RS01620 0.74 +0.6
359,665 - EX31_RS01620 0.87 +0.8
359,743 + -0.1
359,744 - +1.2
359,744 - +1.8
359,747 + +0.4
359,794 - -0.1
359,904 + +0.8
359,904 + -0.6
359,905 - +2.6
359,905 - -1.4
359,928 + +0.6
360,268 + EX31_RS01625 0.24 -1.6
360,306 + EX31_RS01625 0.26 -2.4
360,306 + EX31_RS01625 0.26 +0.2
360,307 - EX31_RS01625 0.26 +0.3

Or see this region's nucleotide sequence