Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08995 and CA265_RS09000 are separated by 249 nucleotides CA265_RS09000 and CA265_RS09005 are separated by 11 nucleotides CA265_RS09005 and CA265_RS09010 are separated by 79 nucleotides
CA265_RS08995: CA265_RS08995 - TonB-dependent receptor, at 2,243,367 to 2,245,793
_RS08995
CA265_RS09000: CA265_RS09000 - hypothetical protein, at 2,246,043 to 2,246,228
_RS09000
CA265_RS09005: CA265_RS09005 - phosphoserine transaminase, at 2,246,240 to 2,247,313
_RS09005
CA265_RS09010: CA265_RS09010 - 3-phosphoglycerate dehydrogenase, at 2,247,393 to 2,248,340
_RS09010
Position (kb)
2246
2247
2248 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2245.290 kb on + strand, within CA265_RS08995 at 2245.338 kb on - strand, within CA265_RS08995 at 2245.450 kb on - strand, within CA265_RS08995 at 2245.451 kb on + strand, within CA265_RS08995 at 2245.462 kb on + strand, within CA265_RS08995 at 2245.493 kb on - strand, within CA265_RS08995 at 2245.545 kb on - strand, within CA265_RS08995 at 2245.552 kb on + strand at 2245.552 kb on + strand at 2245.556 kb on + strand at 2245.634 kb on + strand at 2245.636 kb on + strand at 2245.659 kb on - strand at 2245.687 kb on - strand at 2245.687 kb on - strand at 2245.706 kb on - strand at 2245.710 kb on - strand at 2245.710 kb on - strand at 2245.719 kb on + strand at 2245.719 kb on + strand at 2245.720 kb on - strand at 2245.731 kb on - strand at 2245.734 kb on + strand at 2245.893 kb on - strand at 2245.897 kb on - strand at 2245.950 kb on + strand at 2246.045 kb on - strand at 2246.169 kb on - strand, within CA265_RS09000 at 2246.189 kb on + strand, within CA265_RS09000 at 2246.231 kb on + strand at 2247.347 kb on - strand at 2247.386 kb on + strand at 2247.587 kb on - strand, within CA265_RS09010 at 2247.617 kb on + strand, within CA265_RS09010 at 2247.630 kb on + strand, within CA265_RS09010 at 2247.713 kb on - strand, within CA265_RS09010 at 2247.799 kb on - strand, within CA265_RS09010 at 2247.836 kb on + strand, within CA265_RS09010 at 2247.867 kb on - strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.917 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.030 kb on + strand, within CA265_RS09010 at 2248.100 kb on + strand, within CA265_RS09010 at 2248.101 kb on - strand, within CA265_RS09010 at 2248.156 kb on + strand, within CA265_RS09010 at 2248.259 kb on + strand at 2248.260 kb on - strand at 2248.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,245,290 + CA265_RS08995 0.79 -0.0 2,245,338 - CA265_RS08995 0.81 -0.9 2,245,450 - CA265_RS08995 0.86 -0.8 2,245,451 + CA265_RS08995 0.86 -0.4 2,245,462 + CA265_RS08995 0.86 +1.1 2,245,493 - CA265_RS08995 0.88 +0.1 2,245,545 - CA265_RS08995 0.90 -0.2 2,245,552 + +0.4 2,245,552 + -0.4 2,245,556 + +0.9 2,245,634 + -1.1 2,245,636 + +0.1 2,245,659 - -1.4 2,245,687 - -1.5 2,245,687 - -1.3 2,245,706 - -1.0 2,245,710 - -0.4 2,245,710 - -0.3 2,245,719 + +0.2 2,245,719 + -0.4 2,245,720 - -0.6 2,245,731 - -2.2 2,245,734 + +0.8 2,245,893 - -1.1 2,245,897 - -0.3 2,245,950 + -0.7 2,246,045 - +0.1 2,246,169 - CA265_RS09000 0.68 -4.4 2,246,189 + CA265_RS09000 0.78 -0.5 2,246,231 + -4.1 2,247,347 - -3.3 2,247,386 + -2.4 2,247,587 - CA265_RS09010 0.20 -2.7 2,247,617 + CA265_RS09010 0.24 -0.9 2,247,630 + CA265_RS09010 0.25 -0.6 2,247,713 - CA265_RS09010 0.34 -2.3 2,247,799 - CA265_RS09010 0.43 -3.4 2,247,836 + CA265_RS09010 0.47 -0.2 2,247,867 - CA265_RS09010 0.50 -2.1 2,247,916 + CA265_RS09010 0.55 -1.4 2,247,916 + CA265_RS09010 0.55 -3.5 2,247,916 + CA265_RS09010 0.55 -2.7 2,247,917 - CA265_RS09010 0.55 -0.9 2,248,008 - CA265_RS09010 0.65 -2.1 2,248,008 - CA265_RS09010 0.65 -1.2 2,248,030 + CA265_RS09010 0.67 -3.3 2,248,100 + CA265_RS09010 0.75 -4.2 2,248,101 - CA265_RS09010 0.75 -2.2 2,248,156 + CA265_RS09010 0.80 -1.2 2,248,259 + -1.8 2,248,260 - -3.6 2,248,260 - -2.7
Or see this region's nucleotide sequence