Experiment: L-Tryptophan (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02780 and RR42_RS02785 are separated by 20 nucleotides RR42_RS02785 and RR42_RS02790 are separated by 158 nucleotides
RR42_RS02780: RR42_RS02780 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 619,423 to 620,472
_RS02780
RR42_RS02785: RR42_RS02785 - dimethyladenosine transferase, at 620,493 to 621,314
_RS02785
RR42_RS02790: RR42_RS02790 - tryptophanyl-tRNA synthetase, at 621,473 to 622,495
_RS02790
Position (kb) 620
621
622 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 620.459 kb on + strand at 620.488 kb on + strand at 620.488 kb on + strand at 620.489 kb on - strand at 620.489 kb on - strand at 620.492 kb on + strand at 620.492 kb on + strand at 620.506 kb on + strand at 620.985 kb on + strand, within RR42_RS02785 at 620.985 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.185 kb on + strand, within RR42_RS02785 at 621.186 kb on - strand, within RR42_RS02785 at 621.274 kb on - strand at 621.308 kb on + strand at 621.309 kb on - strand at 621.458 kb on - strand at 621.554 kb on + strand at 621.555 kb on - strand at 621.555 kb on - strand at 621.605 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 621.779 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.160 kb on + strand, within RR42_RS02790 at 622.222 kb on + strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.223 kb on - strand, within RR42_RS02790 at 622.227 kb on - strand, within RR42_RS02790
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (C) remove 620,459 + -0.1 620,488 + -0.6 620,488 + -0.0 620,489 - -3.7 620,489 - -0.9 620,492 + +1.3 620,492 + -0.2 620,506 + -2.1 620,985 + RR42_RS02785 0.60 -1.1 620,985 + RR42_RS02785 0.60 -0.5 621,185 + RR42_RS02785 0.84 -0.1 621,185 + RR42_RS02785 0.84 -1.2 621,186 - RR42_RS02785 0.84 -2.5 621,274 - +0.4 621,308 + +0.2 621,309 - -0.1 621,458 - +1.0 621,554 + -1.3 621,555 - +0.2 621,555 - +0.6 621,605 + RR42_RS02790 0.13 +1.7 621,779 + RR42_RS02790 0.30 -0.1 621,779 + RR42_RS02790 0.30 +0.4 621,779 + RR42_RS02790 0.30 +0.2 621,779 + RR42_RS02790 0.30 +0.2 622,160 + RR42_RS02790 0.67 -0.3 622,160 + RR42_RS02790 0.67 +0.3 622,222 + RR42_RS02790 0.73 +0.5 622,223 - RR42_RS02790 0.73 -0.0 622,223 - RR42_RS02790 0.73 -1.0 622,223 - RR42_RS02790 0.73 -2.5 622,227 - RR42_RS02790 0.74 +0.2
Or see this region's nucleotide sequence